| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,532,298 | 0 | A | T | 100.0% | 691.8 / ‑6.5 | 227 | T15T (ACA→ACT) | femA_3 | Aminoacyltransferase FemA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (87/138); minor base G (0/2); total (87/140) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 228 total aligned reads displayed.
TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAC > USA300TCH1516_ALE/2532167‑2532426 | gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa > 2:1318900/1‑134 (MQ=255) gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTGACTACaaa > 2:811852/1‑134 (MQ=255) gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGAGTTTAATACaaa > 1:2768986/1‑134 (MQ=255) tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTTAATAATTATAGTTCAATTCAGTGATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 1:263368/134‑1 (MQ=255) tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg < 1:515950/134‑1 (MQ=255) aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCa < 2:2289938/134‑1 (MQ=255) tCAGTTTTTGATAAAATAAACAAAGAGCAGTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAt < 2:1877561/134‑1 (MQ=255) tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCTGAGAGGGATCCGATATACTGGCCATCAGTACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAt < 2:2048225/134‑1 (MQ=255) cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGAATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAACAtt > 1:1645821/1‑134 (MQ=255) ttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc > 1:226389/1‑134 (MQ=255) ttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTAAATACAGAGAGGGATGGGATAAACTGAATTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTAGTACAAAGCATTTTTc > 2:1170997/1‑134 (MQ=255) tttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGATGGATTGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc‑a < 1:2406088/134‑1 (MQ=255) tttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGTATATAATGAATTTTGTAACGTTGACTGCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc‑a < 1:635/134‑1 (MQ=255) gATAAAATAAAAAAATAGCATTATTAATATATTCAAAAATTATAGTTCAATTCAGTTAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACAt < 1:1240575/134‑1 (MQ=255) gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTA‑ACAt < 2:2101355/134‑1 (MQ=255) aaaaaaTAGCATAATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATCAGATTGCTACAAAGCATATTTC‑ACATTACACACaa > 2:2737106/1‑134 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAAt > 1:177036/1‑134 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAAt < 1:2237776/134‑1 (MQ=255) aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACATTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAAt > 1:847087/1‑134 (MQ=255) aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGc < 2:168478/134‑1 (MQ=255) attattAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTc < 1:1673036/134‑1 (MQ=255) attattAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTc < 2:968842/134‑1 (MQ=255) attaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCAtt < 2:1265854/134‑1 (MQ=255) tgataaATTCAAGAATTATAGTTCAATTCAGAGAAGGATGGGATAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTAc < 2:658994/132‑1 (MQ=255) aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACa < 2:1060740/134‑1 (MQ=255) ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACaa > 1:2611174/1‑134 (MQ=255) taaATTCATAAATTATAGTTTAATTCATAGATGGATTGGATAAAATGAATTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAAt < 1:478018/134‑1 (MQ=255) taaATTCAAAAATTATAGTTTAATTAATAGAGGGATGGGGTACTACAAACGTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAAt < 1:2837588/134‑1 (MQ=255) aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGTGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCa < 1:2727134/134‑1 (MQ=255) aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCAt < 2:2601529/134‑1 (MQ=255) aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCAt < 2:2790232/134‑1 (MQ=255) ttCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATa < 1:2550252/134‑1 (MQ=255) tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGTGTTTAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATag < 1:41504/134‑1 (MQ=255) tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCATTTCTTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATag > 1:2105084/1‑134 (MQ=255) aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGt > 2:919617/1‑134 (MQ=255) aaaaTTATAGTTCAATTCAGAGAGGGATGCGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGt > 2:1598023/1‑134 (MQ=255) aaaaTTATAGTTCAAAACAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGt > 2:2710013/1‑134 (MQ=255) aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGa < 2:1222667/134‑1 (MQ=255) tataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAtt < 1:947068/134‑1 (MQ=255) ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAttt > 2:2433917/1‑134 (MQ=255) gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTTCAGGGGACAACAGACGAGTAATATTTTTTTGGTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa < 1:396211/134‑1 (MQ=255) aTTCAGAGAGGGATGGGATAAATTGAACTTTATAACTTTGACTTCGGATTAGTTCAATTCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg < 1:2150836/134‑1 (MQ=255) aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg < 2:1431940/134‑1 (MQ=255) aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg < 1:1379211/134‑1 (MQ=255) ttCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGa > 2:1295532/1‑134 (MQ=255) ttCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGa > 1:1817357/1‑134 (MQ=255) cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg < 1:2496620/134‑1 (MQ=255) gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAACAGCCGAtgtg > 2:609911/1‑134 (MQ=255) gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAAAGTTGCTTTACAAGGcactgtt > 2:2861902/1‑128 (MQ=255) agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCTGATGAGTTGAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc < 1:1004123/134‑1 (MQ=255) ggatgggatAAAATGAACTTTGTAACGTTTACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt < 1:358562/134‑1 (MQ=255) ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATTAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt < 1:2356300/134‑1 (MQ=255) gatgagatgaAATGAACTTTGTAATGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGAGATGTGCAtttt < 2:258317/134‑1 (MQ=255) ggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTCCATCTTATAg > 2:1704980/1‑134 (MQ=255) gatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCAGTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg < 1:2137818/134‑1 (MQ=255) gatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAAGGCGTTTACTACAAAGCATTTTTCAACATGA‑ATACGATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg < 2:1752593/134‑1 (MQ=255) atAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATCGAGTTGATTTAAAAGGCGATGTGCATCTTTTCggg > 2:2665659/1‑134 (MQ=255) tAAAATGAACTTTGTAACGTTGACTTCTGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgggg < 1:1112431/134‑1 (MQ=255) tAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACACAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAATGCGATGTGCATCTTGTAcggg > 2:1919486/1‑134 (MQ=255) aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt < 2:2563029/134‑1 (MQ=255) aaaTGAACTTTGTAACGGTGACTTCGGATGAGTTCAATGCGTTTAATACAAAGCATTTTTC‑ACATTACACAGAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt < 2:438512/134‑1 (MQ=255) aaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGATGATATAAAAAGCGATGTGCATCTTGTAGGGGTTa > 1:1093631/1‑134 (MQ=255) aTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAAAGTTGATTTAAAAGGCAATGTACATGTTGTAGGGATTaa > 2:2618104/1‑134 (MQ=255) tatcTTTGTATCGTTTTCTTCGGATTAGTTCAATGCTTTTACTACAAAGCATCTTTA‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg < 1:923829/131‑1 (MQ=255) gAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg < 2:1017446/134‑1 (MQ=255) aCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAt > 2:2681632/1‑134 (MQ=255) cTTTGTAACGTTGACTTCTGATGAGTTCAATTTGTTTACGACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg < 1:2624028/134‑1 (MQ=255) ttGTAACGTTGACTTCGTATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAc < 2:555751/134‑1 (MQ=255) tGTAACGTTGACTTCGGATGAGTTCAATGTGTTTACTATAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACa < 1:1787779/134‑1 (MQ=255) gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTCGAGGCGATAAATATGATaa < 2:631748/134‑1 (MQ=255) gTAACGTTGACTTCGAATGAGTTTAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATTAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa < 2:387400/134‑1 (MQ=255) tAACGTTGACTTCGGATGATTTCAATGCGTTTACTACAAAGCATTTTTC‑ATATTACACACAATCAACTATTCATTACAATCATACAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt < 2:473591/134‑1 (MQ=255) tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTTATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt < 1:2304047/134‑1 (MQ=255) aCGTTGACTTCGGATGAGTTCAATGCGTGTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAAAGTTGATTTAAAAGGCGATGTGCATATTGTAGGGGTTAAAGATGACAATgg > 1:590001/1‑134 (MQ=255) cGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGt < 2:1186670/134‑1 (MQ=255) gTTGACTTTGGATGAGGTAAATGCGTTTACTACAAAGCAATTTTA‑ACATTACAGACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAAGTGCATTTTTTAGGGGTTAAAGATGACAATGGTc < 2:2806054/134‑1 (MQ=255) ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa > 1:1901971/1‑134 (MQ=255) ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGAATCTTGTAGGAGTTAAAGATGACAATCGTCa > 2:2082487/1‑134 (MQ=255) aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑CCCTTCCCCACTCACAGCCATTCTTCACAATCATAGAGTTGATTTAAAAGGCGATTTGCATATTGTAGGAAATAAAGATGACAATGGTCAAgg > 1:675866/1‑133 (MQ=255) cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTATAGGGGTTAAAGATGACAATGGTCAAgca > 2:746237/1‑132 (MQ=255) cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATGCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg < 1:2782537/134‑1 (MQ=255) ttCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGa > 2:881834/1‑134 (MQ=255) ggATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg < 1:1515033/134‑1 (MQ=255) gATTAGTTCAATGCATTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc < 1:2468384/134‑1 (MQ=255) gATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGTGATTCTGTACTGTTATCGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGCTTAAAGATGACAATAGTCAAGTGATTGc > 2:2173856/1‑134 (MQ=255) gATGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc < 1:207896/134‑1 (MQ=255) aTGAGTTCAATGCGTTTACTACAAAGCATTTTTT‑ACATTACACACAATCAGCTATTCATTTCAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTACGGGTTAAAGATGACAATGGTCAAGTGATTGCa < 2:2538048/134‑1 (MQ=255) tGAGTTCAATGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGACGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAg > 2:2316128/1‑134 (MQ=255) ctaTGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGGTAAAGATGACAATGGTCAAGTGATTGCAGGATGCt < 1:736155/132‑1 (MQ=255) aTGCGTTTACTACAATTCATTTTTC‑ACATTACACACAATCAGTTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa < 1:2710013/134‑1 (MQ=255) aTGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGTCATCGTTGACAGGCATAGAGTTGAATCAAAAGACGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa > 1:1970942/1‑134 (MQ=255) aTGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa < 1:1587312/134‑1 (MQ=255) aTGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa > 1:2660998/1‑134 (MQ=255) aTGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa < 1:919617/134‑1 (MQ=255) aTGCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGAGGTTAAAGATGACAATGGTCAAGTGATTGAAGGATGCTTa > 2:915286/1‑134 (MQ=255) gCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt < 1:1420317/134‑1 (MQ=255) gCGTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCAAAGAGTTGATTTAAAAAACGATGTGCATATTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt > 1:69076/1‑134 (MQ=255) gTTTACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa > 2:1495407/1‑134 (MQ=255) tttACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGATTAAAGATGACAATGATCAAGTGATTGCAGGATGCTTATTCAc > 2:1950933/1‑134 (MQ=255) tttACTACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGATGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTAACGTGGTTGCAGGATGCTTATTGAc > 1:1465281/1‑134 (MQ=255) | TTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTC‑ACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAC > USA300TCH1516_ALE/2532167‑2532426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |