BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,930	C→T	6.2%	S752S (TCG→TCA)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,930	0	C	T	6.2%	991.3 / 6.9	439	S752S (TCG→TCA)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base C (172/210); new base T (18/26); total (197/242)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 447 total aligned reads displayed.

TGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGC  >  USA300TCH1516_ALE/2774798‑2775063

 gggtctgagtcGCTATCGGAGTGTGAGTCGCTGTCTGAATCTGAATCACTGTCTGACTCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGa                                                                                                                                     <  2:563485/132‑1 (MQ=17)

  agtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTTAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGaa                                                                                                                                    <  1:1180932/134‑1 (MQ=255)

    tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑Ctgagtctgagtc                                                                                                                                  >  2:2075411/1‑134 (MQ=255)

    tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATc                                                                                                                                  <  2:1207238/134‑1 (MQ=255)

     ctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTATCTGAATCTGAATCGCTGT‑ATGAGTCCGAAtag                                                                                                                                 >  2:906163/1‑132 (MQ=255)

       gagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCt                                                                                                                               <  1:2771295/134‑1 (MQ=255)

          tcGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTatc                                                                                                                            <  2:2322224/134‑1 (MQ=255)

            gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTAT‑CTGCGTCCGACTCGCTgtctg                                                                                                                          >  2:644763/1‑134 (MQ=14)

             cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTatctga                                                                                                                         >  1:722112/1‑134 (MQ=255)

                tcggtgTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTatctgaatc                                                                                                                      <  2:516845/129‑1 (MQ=255)

                  cGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGACTCGCTagcagaatctg                                                                                                                    >  1:516237/1‑134 (MQ=255)

                  cGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTatctgaatctg                                                                                                                    >  1:1593618/1‑134 (MQ=255)

                   gAGTTTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCTAAGCGCTATGTGAAGCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTatctgaatctga                                                                                                                   <  2:873691/134‑1 (MQ=17)

                   caGTGTGAGTCGGTTTCTGAATCCTAATCACTGTCTGAATCCGAATCGCTATCTGAACCTTAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTatctgaatctga                                                                                                                   <  2:1073857/133‑1 (MQ=17)

                     gTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGACTCTGAGt                                                                                                                 >  2:2453088/1‑134 (MQ=255)

                       cTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCg                                                                                                               <  2:1380846/134‑1 (MQ=255)

                          aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                                                                                            <  1:2292700/134‑1 (MQ=255)

                          aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                                                                                            >  1:432919/1‑134 (MQ=255)

                           gTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgt                                                                                                           >  1:467979/1‑134 (MQ=255)

                           ctCGCTGTCTGAAGCTGAATCTCTGTCTGAATCCGAGTCGCTATCTGAATCTGAAGCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgt                                                                                                           <  1:594342/133‑1 (MQ=255)

                            tCGCTGTCTGAATCTGAATCACTGTCTGAATCTGAATCGCTATCTGAATCTGAAGCGCTATCCGAGTCGGAGTCGCTGTCTGCATCTGAATCGCTGT‑ATGAGTCTGAATCGCAATATGATTCTGCGTCGccttc                                                                                                          >  2:1699080/1‑130 (MQ=14)

                            tCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtc                                                                                                          >  1:2141957/1‑134 (MQ=255)

                             ctcTGTCTGAATCTGAATCACTGTCTGAATGTGAATCGCTATCTGATTCTGAATCGCTATCAGAGTCGGAGTCGCTGGCTGAAGCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                                                                                                         <  1:993692/132‑1 (MQ=17)

                             cGCTGTCTGAATCTGAATCATTATCTGAATCCGAATCTCTATCTGTTTCTGTATCGCTTTTCGAGTTTGTGTCGCTTTGTGAGGGTGGGTCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                                                                                                         <  1:1435608/134‑1 (MQ=11)

                              gCTGTCTGAATCTGACTCACTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                                                                        <  2:393295/134‑1 (MQ=11)

                               cTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑ATGAGTAAGAATCGCTATCTGAATCTGAGTCGCTgtctga                                                                                                       >  1:140210/1‑134 (MQ=255)

                                 gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTgtctgagt                                                                                                     <  1:17585/134‑1 (MQ=255)

                                 gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAAACGCAATCTGAATCTGAATCGCTgtctgagt                                                                                                     >  2:34692/1‑134 (MQ=255)

                                  tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                    >  1:2057247/1‑134 (MQ=255)

                                    tgaatctgaatcACTGTCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                  <  1:1194067/134‑1 (MQ=255)

                                    tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                  >  1:2397392/1‑134 (MQ=255)

                                     gagtctgaatcGCTGTCTGATTCTGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctga                                                                                                 <  2:2420779/131‑1 (MQ=14)

                                        tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                                                                                              >  1:2580307/1‑134 (MQ=255)

                                         ctgaatcACTGTCTGAATCCAAATGGCTATCTGAATCTGAATAATAAGCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCg                                                                                             <  1:2090567/134‑1 (MQ=17)

                                           gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTCAGTCCGAATCGCTATCTGAATATAAGTCGCTCTCCGCATCTGAATCGCt                                                                                           >  2:420371/1‑134 (MQ=17)

                                              tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATGCGAGTCTGAGTCGCTGTATGAGTCTGAATCACCTT‑CTGAGTCGCCGTCGCTTTCTAAATCTGAGTCGCTGTCTGAGTCGGAATCGCGatc                                                                                        >  2:2039777/1‑134 (MQ=14)

                                              tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                        >  1:1563157/1‑134 (MQ=255)

                                                 cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATTCGTGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                     <  1:1005241/134‑1 (MQ=255)

                                                   ttCTGAGTCGGAATCGCGATGGCTATCTGAATCGCTATCTGAGTCTGAGTAGCTGTCGGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                   <  2:1858119/133‑1 (MQ=11)

                                                   gTCTGAATCCTAATCGCTATCTGAATCTGAATTTCTATCCTATTCTGAGTCTCTATCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                   <  1:2017703/134‑1 (MQ=14)

                                                   gTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTTGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                   <  1:801537/134‑1 (MQ=255)

                                                   atCTGATTCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                   <  2:2243834/133‑1 (MQ=255)

                                                    tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGACTCCGACTCGCTATCAGAATCTGAGTCGCTCTCTGAGCCTGAATCACTgtctgaatc                                                                                  >  2:1112396/1‑134 (MQ=14)

                                                     cTGAATCCGAATCGCTATCTGAATCTGAACAGTCCTGCGATTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct                                                                                 <  2:1287758/134‑1 (MQ=14)

                                                      tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATACTTGTGCAGCGGCC‑AATCACTATCTGAATCTGAGGCGCTGTCTGAGTCTGAATCACTatctgaatctg                                                                                >  1:667820/1‑134 (MQ=17)

                                                        aaTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAACCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                              <  1:1532029/134‑1 (MQ=255)

                                                          tcggattCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                            <  2:2836224/128‑1 (MQ=255)

                                                          tCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                            <  1:1449871/134‑1 (MQ=255)

                                                          tCCGAATCGCAATATGAGTCGGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                            <  1:1794759/134‑1 (MQ=255)

                                                           ccGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCCCTGT‑CTGAGTCCGAATAGATATCTGAATCTGAGTCGCTATCTGAATCTGAATCGCTctctgcttctgaaaca                                                                           >  1:377803/1‑130 (MQ=14)

                                                            cGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAATAGCTATATGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                                                          >  1:2313190/1‑134 (MQ=255)

                                                              aaTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑TTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTa                                                                        <  1:1699294/134‑1 (MQ=255)

                                                               aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGCATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAt                                                                       >  1:2264290/1‑134 (MQ=255)

                                                               aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCCAGTTTCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAt                                                                       >  1:2851663/1‑134 (MQ=255)

                                                                 cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTAACTCCGGATCGCTATCTGAATCTGATTAGCTGTCTGAGTCTGAATCGCTATCTGCATATGAGTCGCTTtct                                                                     >  1:1320305/1‑134 (MQ=14)

                                                                  gCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGT‑CTGAGTGCGAATCGCTATATGGATCTGAGGCGCTGTCTGAGTCTGAATCGCTATCTGCATCTGAGTCGCTAtctc                                                                    >  2:2640340/1‑133 (MQ=14)

                                                                   cTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                            >  1:1482270/1‑125 (MQ=255)

                                                                      tctgaatctgacTCGTTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAAGCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                <  2:2394586/134‑1 (MQ=255)

                                                                            tctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTTCGAATTGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                          <  1:1011530/134‑1 (MQ=255)

                                                                              tgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                        >  1:457535/1‑134 (MQ=255)

                                                                               gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGGATCGCTATCTGAATCTGAGTCGCTAtctgagactgattcgct                                                       >  2:1652975/1‑134 (MQ=255)

                                                                                aatcGCTATCCGAGTCTGAGTCTCTGTCTGAATCTGAATCGCTGT‑CTGAGTCTGAATCTCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctg                                                      <  1:1870234/134‑1 (MQ=255)

                                                                                aatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTAtctgagtccgagtcgcta                                                      >  2:1969434/1‑133 (MQ=255)

                                                                                  tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGTATCGCTGT‑CTGAGTTCTTATCGCTATCTGAATCTGTGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc                                                    <  1:821661/134‑1 (MQ=14)

                                                                                    gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtcgg                                                  >  1:768213/1‑132 (MQ=255)

                                                                                         ccGAGTCTGAGTCGCTGTCTGACTCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGGTATCTGAATCGGAGTCGCTAtctgagtctgagtcgctgtctgagtct                                             <  1:267920/134‑1 (MQ=255)

                                                                                           gAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctga                                           >  2:1345895/1‑134 (MQ=255)

                                                                                            attCTGTGTCGTTGTCTGAATCTGAATCGCTGT‑CTTAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                          <  1:1267330/132‑1 (MQ=255)

                                                                                            aGTCTGAGTCGCTGTCTGAATCTGAATCGTTGT‑CTGAGTTCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgag                                          <  1:536002/134‑1 (MQ=255)

                                                                                              tCTGAGTCGTTGTCTGAATCTGAATCTCTGT‑CTGAGTCTGAATCTCTATCTGAATCTTAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                        <  1:1680103/134‑1 (MQ=14)

                                                                                               cggAGTAGCTGGCTTAATCTTGTGCGCTAC‑CTGGGTCTGAAGCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGATGCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcg                                       <  2:2535413/132‑1 (MQ=14)

                                                                                                 gAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                     <  1:1149681/134‑1 (MQ=255)

                                                                                                  aGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCTGAATCGCTATCTGAATCTGAGTCGTTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgctg                                    <  1:2616309/134‑1 (MQ=255)

                                                                                                   gTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATctgagtctgagtcgctgtctgagtctgagtcgctgt                                   <  2:2580307/134‑1 (MQ=255)

                                                                                                     cgttatctgaatctgagTCGCTAT‑TTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                                 <  2:1237893/129‑1 (MQ=11)

                                                                                                     cGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                                 <  1:2453088/134‑1 (MQ=255)

                                                                                                      gCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGagtctgagtcgctgtctgagtctgagtcgctgtctg                                <  1:2492699/134‑1 (MQ=255)

                                                                                                         gTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTctgagtcgctgtctgagtctgagtcgctgtctgagt                             >  1:939452/1‑134 (MQ=255)

                                                                                                          tctgaatctgaaTCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctttctgattctgagtcgatgtctgagtc                            >  1:1979396/1‑134 (MQ=255)

                                                                                                           ctgaatctgaatCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCCGTATCTTAGTCTgagtcactgtctgagtctgaggcgctgtctgagtct                           >  2:805661/1‑134 (MQ=14)

                                                                                                            tgtcgctggatcGCTGT‑CTTAGTCCGAGTCGCTATCTGAATCTGAGTCGCTTTCTGAGTCTGAGTCGCTTTCTGATTCTGAGTCGCTATCTGAGTCTGagtcgctgtctgagtctgagtcgctgtctgagtctg                          <  2:1485961/129‑1 (MQ=14)

                                                                                                             gagtctgaatcGCTGT‑CTGAGTCCGAGTCGCTTTCTGAATCTGAGTCGCTGTCTGATTCTGAGTCGCTGCCTGAGTCTGAGTCGCTATCTGAGTCTGAgtcgctatccgagtctgaatcgctgtctgagtctga                         <  2:1579760/131‑1 (MQ=11)

                                                                                                               atctgaatcGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAt                       <  2:2307921/134‑1 (MQ=255)

                                                                                                                  tgactcGTTGT‑CTGAGGCGGAATCGCTATCTGAATCTGAGTCGCTGTCTGATTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGc                    <  2:2713047/134‑1 (MQ=14)

                                                                                                                  tgaatcGCTGT‑CTGAGTCCGAATCGCTTTCTGAATCTGAGTCGCTGTTTGATTCTGAATCGCTATCTTAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGc                    <  1:1088888/134‑1 (MQ=255)

                                                                                                                  tgaatcGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGc                    <  1:1511506/134‑1 (MQ=255)

                                                                                                                  tgaatcGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGc                    <  1:1646242/134‑1 (MQ=255)

                                                                                                                  tgaatcACCTT‑CTGAGTCCGTATCGCTATCTGTATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGGCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGc                    <  1:335564/134‑1 (MQ=17)

                                                                                                                  tgaagcGCTGT‑CTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGGCTGAGTCGCTGTCTGAGTCTGAATCGc                    <  1:1246519/134‑1 (MQ=255)

                                                                                                                   gattcGCTGT‑CTGAGTCCGGATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                   <  2:958051/131‑1 (MQ=255)

                                                                                                                   gaatcGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaaacact                   >  1:2539235/1‑129 (MQ=255)

                                                                                                                   gaatcGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                   <  1:874426/134‑1 (MQ=255)

                                                                                                                      tcGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                <  1:2787319/134‑1 (MQ=255)

                                                                                                                       cGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGCCTGAATCGATatct               >  2:2132827/1‑134 (MQ=255)

                                                                                                                         cTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga             >  1:1905639/1‑134 (MQ=255)

                                                                                                                           gt‑ctgagtctgaCTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTGGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat           <  2:2871064/134‑1 (MQ=14)

                                                                                                                            t‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc          <  1:1633603/134‑1 (MQ=255)

                                                                                                                                 aGTCCGGATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg      <  1:1296343/134‑1 (MQ=255)

                                                                                                                                   tCCTAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGACTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc    <  2:467979/134‑1 (MQ=255)

                                                                                                                                   tCCGAAGCGCTATCTGAATCTGTTTCGTTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGGGTCTGAATCGCTATCTGAATCTGAGTc    <  2:709461/134‑1 (MQ=14)

TGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGT‑CTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGC  >  USA300TCH1516_ALE/2774798‑2775063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`