BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,660	T→A	11.0%	I173I (ATT→ATA)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,660	0	T	A	11.0%	589.7 / 45.8	228	I173I (ATT→ATA)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (73/128); new base A (10/16); total (84/144)
Fisher's exact test for biased strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

GTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCA  >  USA300TCH1516_ALE/110532‑110792

 aagATAATAAAATCATTAAAAAAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCatat                                                                                                                                <  2:659481/132‑1 (MQ=255)

     taataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATc                                                                                                                            <  1:2360582/134‑1 (MQ=255)

           aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                      <  1:1797853/134‑1 (MQ=255)

            aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagaact                                                                                                                     >  2:1502025/1‑131 (MQ=255)

            aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGt                                                                                                                     <  2:1151224/134‑1 (MQ=255)

            aTCATACCAAAAAAAAAAATTAAATACTAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGt                                                                                                                     <  2:289907/134‑1 (MQ=17)

               aTTTCAACAAAAGAAATTAAAGATTAAATAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                  <  2:95528/134‑1 (MQ=255)

                 tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGATATCTAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                <  1:1934977/134‑1 (MQ=255)

                 tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAACCTTTAAGTTCTTTTTTAAATATGGAGAATTTAAAAATTAGAAAAATAATAAAGACGGAGATATTTAATATAATACAGAGGTGCCGa                                                                                                                >  1:1049646/1‑134 (MQ=17)

                 tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAATTCTATATACAATATAGCAACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                >  1:54644/1‑134 (MQ=17)

                 acaaaaaaaaTAAATCAAATATGAATAAAATTTAATATACATCGTAAACTGTAAGTTCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTAGAAACGCGGATATATTTCATATAATTCAGAGGTGCCGa                                                                                                                <  2:199344/128‑1 (MQ=255)

                  caaagtaaaGAAATTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAATATGGATATATTTCATATAATCCAGAGGTGCCGAg                                                                                                               <  2:2006477/128‑1 (MQ=17)

                   caacaaCACAAATCAAAGATGTAAAAAGCAAAAAAGAAATCAAAAAATTTAAGTTCTCTATTCAATATCGCGATATAAAAAATAAAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                              <  2:914852/134‑1 (MQ=17)

                   caacaaAAGAAATTAAAGTTGACTTATTTTAAATAGTTTTCGTAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                              <  1:392302/134‑1 (MQ=17)

                   caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGaat                                                                                                              <  2:2830036/134‑3 (MQ=255)

                    aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACATTAAGTTCTTTATTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACTGAGATATTTCATATAATCCAGAGATGCAGAttt                                                                                                             >  2:2509666/1‑134 (MQ=17)

                          aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                       <  1:888661/134‑1 (MQ=255)

                            aaaaTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                     <  2:207914/134‑1 (MQ=255)

                            aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                     >  1:2789522/1‑134 (MQ=255)

                            aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGAGACTTTAAAAATTTGAAAAAGTATAAAGACAGAGATATTTAATATTATCCAGAGTTGCCGAATTATTCGGCt                                                                                                     >  2:834092/1‑134 (MQ=14)

                            aaaTTAAAGATAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                     <  2:2855089/134‑1 (MQ=255)

                                tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATTGTGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                 <  1:136509/134‑1 (MQ=255)

                                      tGAAAAAATAAAAAAATAAATCGAAAACTTTAAGTTCTTTGTTCAATTTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAt                                                                                           <  1:869425/134‑1 (MQ=255)

                                          aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                       <  2:1561021/134‑1 (MQ=255)

                                          aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                       >  1:2280194/1‑134 (MQ=255)

                                          aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTATTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTCGGCTTAATCTCAATTTAc                                                                                       >  2:587579/1‑134 (MQ=17)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAATAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                      <  2:1018706/134‑1 (MQ=255)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                      <  1:918114/134‑1 (MQ=255)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                      <  1:1548575/134‑1 (MQ=255)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                      >  1:2077241/1‑134 (MQ=255)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATACAGAGGTGTCGAGTGATTCGGCTAAATATCAATTAACt                                                                                      <  1:2730481/134‑1 (MQ=255)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTACCGAGTTATTCGGCTAATTATCAATTAACt                                                                                      >  2:2542284/1‑134 (MQ=255)

                                           aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTATGTTCAATATGGCGAATTTAAAAATTTAAAAAATTATAAAGACGGAGATATTTAATATAATACAGAGGAGAAGAGATATTCGCCTAAATATCAATTaaat                                                                                      >  2:483630/1‑132 (MQ=17)

                                            gataataTAAGAAATCTAATACCTTAAGTTCTTTGTTCAATTTGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                     <  2:2087968/133‑1 (MQ=17)

                                              tAAAAAAAGAAATCGAAAACATTAAGTTCTTTGTTCAACCTGGCGTCTATAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                   <  2:2784249/134‑1 (MQ=17)

                                               aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAAGTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                  <  2:192654/134‑1 (MQ=255)

                                               aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                  >  1:2614028/1‑134 (MQ=255)

                                                    aaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                             >  1:2205619/1‑134 (MQ=255)

                                                       aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                          <  1:636958/134‑1 (MQ=255)

                                                        aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                         <  1:1589/134‑1 (MQ=255)

                                                          tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAAt                                                                       <  2:1658271/134‑1 (MQ=255)

                                                             aaaaCATTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTTAAAAATTATAAAGACGGAGATATTTCATATAATCCATAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTa                                                                    <  1:953843/134‑1 (MQ=255)

                                                              aaattgtAAGTGCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                   <  2:1710164/128‑1 (MQ=255)

                                                              aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTTATATATTTCATAGCTGCTGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                   <  2:1751008/134‑1 (MQ=17)

                                                              aaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                   <  2:1382072/134‑1 (MQ=255)

                                                              aaaCTTTAAGTTATTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                   <  2:2145328/134‑1 (MQ=255)

                                                                     aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                            >  1:707787/1‑134 (MQ=255)

                                                                      aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGTAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATCATTATAATGTAAAACAATTa                                                           >  2:2463771/1‑134 (MQ=255)

                                                                        ttCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                         >  2:1207351/1‑134 (MQ=255)

                                                                         tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                        <  1:1446682/134‑1 (MQ=255)

                                                                         tCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                        <  2:1849702/134‑1 (MQ=255)

                                                                          cTTTGTTCAATATGGCGACTTTAAAAATTTGAACAATTATAAAGACGGAGATATTTCATATAATAAAGAGGTACCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                       >  1:722311/1‑134 (MQ=255)

                                                                          cTTTGTTCAATATGGCGACTTTAAAAATTCGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                       <  1:1673688/134‑1 (MQ=255)

                                                                           tttGTGTAATTTGACGACTTTAAAAATTTGAAAAATTATAAAGACTGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                      <  2:1681300/134‑1 (MQ=17)

                                                                            ttGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAAACGGAGATATTTCATATAAACCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                     <  1:215458/134‑1 (MQ=255)

                                                                            ttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                     <  1:1532852/134‑1 (MQ=255)

                                                                              gTTCAATATGGCGACATTAAAAATTAGAAAAATTATAAAGACGGAGATATTTCATATAATCCAAAGTTTCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAg                                                   >  2:879917/1‑134 (MQ=17)

                                                                                tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                 <  1:2428551/134‑1 (MQ=255)

                                                                                tCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATAGAATCCAGAGGTGCTGAGCTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                 <  1:1649244/134‑1 (MQ=255)

                                                                                 cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                                >  1:1019734/1‑134 (MQ=255)

                                                                                 cAATATGGCGACTCTATAAAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                                >  2:1766407/1‑134 (MQ=255)

                                                                                   atatGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatg                                              >  2:689315/1‑134 (MQ=255)

                                                                                      tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATTCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                           <  1:497581/134‑1 (MQ=255)

                                                                                      tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                           <  2:1802979/134‑1 (MQ=255)

                                                                                      tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                           <  2:374596/134‑1 (MQ=255)

                                                                                      tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                           <  2:314632/134‑1 (MQ=255)

                                                                                        gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                         >  1:2696154/1‑134 (MQ=255)

                                                                                         ctaCTTTAAAAATTTGAAAAATTATAAAGACCGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATACTGTAAGTCAAAGCTTCAAAAgatatgatattc                                        <  2:632259/132‑3 (MQ=17)

                                                                                            tttgaaaaaTTTGAATAATTATAAAGACGTAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                     <  2:440423/130‑1 (MQ=255)

                                                                                            cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcga                                     <  1:2440509/134‑1 (MQ=255)

                                                                                              ttaaaaattttaaaaattATAAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                   <  1:2050959/134‑1 (MQ=255)

                                                                                                 aaaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATAAACTAATGATGATTATCATGTAAAACAATTACGCAAAAGATATGATATACAGACGAGt                                >  1:2228390/1‑134 (MQ=255)

                                                                                                  aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCTACGAGTa                               >  2:1100854/1‑134 (MQ=255)

                                                                                                  aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                               >  1:2026740/1‑134 (MQ=255)

                                                                                                   aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGGTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAGAACAATTACGCAAAAGATATGATATACCTACGAGTaa                              >  2:930029/1‑134 (MQ=255)

                                                                                                   aaTTTGAAAAATTATAAAGACGGAGATATGTCATATAATCGAGAGGTGCCGAGATATTCGGCTAAATATCAATTAAATAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATCTCCGAACGAGtca                              >  2:2348577/1‑132 (MQ=17)

                                                                                                     tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                            <  1:2508000/134‑1 (MQ=255)

                                                                                                      ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                           <  1:1113060/134‑1 (MQ=255)

                                                                                                       tGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                          <  2:54644/134‑1 (MQ=255)

                                                                                                         attaaTTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                        <  2:859573/131‑1 (MQ=255)

                                                                                                         atgaaTTATAAAGATGGAGATATATCATATAATCCATATGTGCCGATTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                        <  1:579498/131‑1 (MQ=14)

                                                                                                         aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                        <  1:29659/134‑1 (MQ=255)

                                                                                                         aaaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                        >  2:1114422/1‑134 (MQ=255)

                                                                                                            aaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                     <  1:2607642/134‑1 (MQ=255)

                                                                                                                 taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                <  2:7259/134‑1 (MQ=255)

                                                                                                                 taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGATAAATATCAATTCACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                >  2:2498081/1‑134 (MQ=17)

                                                                                                                    aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt             <  1:985836/134‑1 (MQ=255)

                                                                                                                    aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt             <  1:1502025/134‑1 (MQ=255)

                                                                                                                     gACGGAGATATTTCATATAATCCAGAGCTGCCGAGTTATTCGGCTAAATATCAATTATCTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt            <  1:1572948/134‑1 (MQ=255)

                                                                                                                      atggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCTGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTa           <  1:1939634/132‑1 (MQ=255)

                                                                                                                       tggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCAACGAGAAAAGCACCAAAGTTATTGTTaa          >  1:1210880/2‑134 (MQ=14)

                                                                                                                          agaTATATCATATAATCCAGAGGTGGCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACGATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg       <  2:1354866/134‑1 (MQ=255)

                                                                                                                           gaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg      <  2:2274120/134‑1 (MQ=255)

                                                                                                                           gaTATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAATTTTTATGATAAAAAGATGATATACAGACAAGTAAAGCTCAAAAGTTATTGTTAAAAgg      >  1:1348166/1‑134 (MQ=17)

                                                                                                                            atatatCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt     >  1:416949/1‑134 (MQ=255)

                                                                                                                            atatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATTATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt     <  1:1280807/134‑1 (MQ=255)

                                                                                                                            atatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt     >  2:2245839/1‑134 (MQ=255)

                                                                                                                            atatTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt     <  2:1247352/134‑1 (MQ=255)

                                                                                                                              atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAACAACGAGTAAAGCTCCAAAATTATACTAAAAAggata   >  1:2036763/1‑131 (MQ=17)

                                                                                                                               tttCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGAAGATTATAATGTAACACGATGACGTAAAAGATATGATATACCGACGAATAACGCTCCAAAGTTATTGTTAAAAGGTTCa  >  2:334539/1‑134 (MQ=17)

GTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCA  >  USA300TCH1516_ALE/110532‑110792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`