BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	111,560	T→C	7.1%	R210R (CGT→CGC)	USA300HOU_RS00520 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	111,560	0	T	C	7.1%	472.7 / 15.7	172	R210R (CGT→CGC)	USA300HOU_RS00520	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base T (95/62); new base C (5/7); total (103/69)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

TTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTA  >  USA300TCH1516_ALE/111427‑111691

tttttGCGCAATTTTTATATTTTATATTTTTTATTTATTATTAAGATGGAGATATATCACATAACCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGt                                                                                                                                     <  1:741825/134‑1 (MQ=14)

 ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTa                                                                                                                                    <  1:1647427/134‑1 (MQ=255)

   ttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaa                                                                                                                                  >  1:283321/1‑134 (MQ=255)

    tgtacAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                                                                                                 <  2:2711115/130‑1 (MQ=14)

      cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGTAGATATATCATATAATCCAGAGGTGCCTAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                                                               <  1:786746/134‑1 (MQ=255)

      cgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATCATCACAAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTACAACACTTCCGCAAACGa                                                                                                                               >  2:2501094/1‑134 (MQ=17)

       gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGat                                                                                                                              >  2:492182/1‑134 (MQ=255)

       gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGat                                                                                                                              >  1:2133911/1‑134 (MQ=255)

          atatAGCAATTTTAAAGATTTAATGAATTGTTATAACTAAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATg                                                                                                                           <  1:1957346/134‑1 (MQ=18)

          atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATg                                                                                                                           >  1:2612494/1‑134 (MQ=255)

           tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATATTTCAGAGGTGCCGAGTTATTCGGATAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                                                                          <  1:250825/134‑1 (MQ=14)

           tataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATTATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                                                                          <  1:1000925/134‑1 (MQ=255)

           tataGCAAGTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGa                                                                                                                          <  2:1898899/134‑1 (MQ=255)

                cAATTTTAAAGATTTAATGAATTATAAAGATGGATATATATCATATAATCCAGAGTTGCCGAGTTATTCATCTTAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAc                                                                                                                     <  1:405075/134‑1 (MQ=255)

                cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                                                                                                     <  1:1883497/134‑1 (MQ=11)

                     ttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTACAACAATTACGCAAAAGATATGATATAccgacg                                                                                                                <  2:493594/134‑5 (MQ=11)

                           aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                                                          <  1:1849754/134‑1 (MQ=255)

                            tttAATGAATTATAAAGATGGAGATATATCATATAATCCATAAATTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaaa                                                                                                         >  2:624353/1‑133 (MQ=255)

                                aTGAATTATAAAGATTGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATTTATTAACTAATTATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                                                                     <  1:52226/134‑1 (MQ=255)

                                  gAAGTATATAGAGGTGGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCGCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAATAGATATGACATACCAACAAATAAAGCGCCGa                                                                                                   <  2:586221/134‑1 (MQ=14)

                                   aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGTTTTTGAAGTAAAACAAATACGTAAAAGATATGACATACCAACAAATAAAGCGCCGaa                                                                                                  >  1:662317/1‑134 (MQ=17)

                                    aTTATAAATATTGATTTATATTATATAATTTAGAGGTGCTTATTTATTTAGCTTAATATCAATTAATTAATGATGATTATAATTTAAAACAATTACGTAAAAGATATTACATACCAACAAATAAAGCGCCGAAg                                                                                                 <  2:2270875/134‑1 (MQ=14)

                                    aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATAACATACCAACAAATAAAACGCCGAAg                                                                                                 >  1:645875/1‑134 (MQ=255)

                                         aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                                                                            >  1:2340801/1‑134 (MQ=255)

                                         aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgtt                                                                                            <  2:172794/134‑1 (MQ=255)

                                           aGATGGAGATATATCATATTATCCAGAGGTTCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAGATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                                                          <  1:83068/134‑1 (MQ=255)

                                              tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                                                                       >  1:480260/1‑134 (MQ=255)

                                               ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAATCAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaa                                                                                      <  1:1264058/134‑1 (MQ=255)

                                               ggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAGCAATTACGAAAAAGAAATGACATACCAACAAATAAAGCGCAGAAGATGTTGTTGaa                                                                                      >  1:398207/1‑134 (MQ=14)

                                                  gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAATAATGATGATTCTAATGTAAAACAATTACGTAAAAAATAAGACATACTAACCAATCAAGCGCCGAAACTGTTGTTGCAAgt                                                                                   >  2:1217724/1‑133 (MQ=17)

                                                   atatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTACCTAATGATGATTACAATGTAAAACAATTACGTAAAAGATATTACATCCCAACAAATACAGCGCCGAAGCAGTTGTTCAAAgct                                                                                  >  2:2807684/1‑132 (MQ=14)

                                                    tatatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTa                                                                                 >  2:1081164/1‑134 (MQ=255)

                                                     atatCATATAATCCAGCGGTGCTGAGGTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                >  2:1045489/1‑134 (MQ=255)

                                                     atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                <  2:324350/134‑1 (MQ=255)

                                                     atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                                                                >  1:1650909/1‑134 (MQ=255)

                                                        tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATCCCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAga                                                                             >  2:2377460/1‑133 (MQ=255)

                                                          atatAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGa                                                                           <  1:1857819/134‑1 (MQ=255)

                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATAGTAAACAATAAAGCGCCGAAGCTGTTGTTAAAAGGAAAAGGGAAtata                                                                      >  2:2161146/1‑134 (MQ=17)

                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                                                      >  1:1291648/1‑134 (MQ=255)

                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTa                                                                      >  1:175892/1‑134 (MQ=255)

                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAGGGAATTTa                                                                      >  1:1319238/1‑134 (MQ=255)

                                                               aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAAAGACATACAAACAAATAAAGCGCCGAAGCAGTTGTTGAAAGGTACAGGGAATTTa                                                                      >  1:909338/1‑134 (MQ=255)

                                                                tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAACTATTATTGAAAGGTACAGGGAAATTaa                                                                     >  1:1990668/1‑134 (MQ=255)

                                                                 ccAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTaaa                                                                    >  2:2206938/1‑134 (MQ=255)

                                                                 ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCAAAGCTGTTGATGAAAGGTACAGGGAATTTaaa                                                                    >  1:2046139/1‑134 (MQ=255)

                                                                 ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAAAAAATAAAGCGCCGAAGCTGTTGTTAAAAGGTACAGGGAATTTaaa                                                                    >  2:2337979/1‑134 (MQ=255)

                                                                   agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAg                                                                  >  2:2526673/1‑134 (MQ=255)

                                                                   agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAg                                                                  >  2:641057/1‑134 (MQ=255)

                                                                    gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCCGTTGTTCAAAGGTACAGGGACTTTAAAAgg                                                                 >  2:1260065/1‑134 (MQ=255)

                                                                     agGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                                                                >  1:115099/1‑134 (MQ=255)

                                                                      ggTGCCGAGTTATTCAGCTCAATATCCATTAACTAATGATGATTATAATGTCAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCAGAAGCAGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                                                               >  1:1405331/1‑134 (MQ=255)

                                                                      ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAGGGAATTTAAAAGGtt                                                               >  1:2391947/1‑134 (MQ=255)

                                                                       gTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCAAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtc                                                              >  2:1377998/1‑134 (MQ=255)

                                                                        tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                             <  1:1028161/134‑1 (MQ=255)

                                                                        tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                             <  1:838782/134‑1 (MQ=255)

                                                                        tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                                                             <  1:1951818/134‑1 (MQ=255)

                                                                         gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                                                            <  1:2041097/134‑1 (MQ=255)

                                                                         gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                                                            <  2:2435819/134‑1 (MQ=255)

                                                                         gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAAAAAAGAGACGAAAATATTAATAAAAGATACAGGGAATGTAAAAGGTtgat                                                            >  1:2304902/1‑134 (MQ=14)

                                                                         gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATAGGACATACTCACCAACAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                                                            >  2:1301461/1‑134 (MQ=17)

                                                                            gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg                                                         >  2:1801614/1‑134 (MQ=255)

                                                                             aGTTATTCAGCTCCATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAGCAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATGTAAAAGGATCATCAGt                                                        >  2:340784/1‑134 (MQ=255)

                                                                                 aatCAGATCAATATGAATTAACCAATGATGATTATAATATAAAACAATTACGTAAAAGATATGACATACGAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGAGAATTTAAAAGGTTCATCAGTTGGa                                                    <  2:1965385/132‑1 (MQ=14)

                                                                                   tCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACCAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata                                                  >  1:1529318/1‑134 (MQ=255)

                                                                                     aGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata                                                >  2:57010/1‑134 (MQ=255)

                                                                                           atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa                                          <  1:1589969/134‑1 (MQ=255)

                                                                                           atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa                                          <  2:258872/134‑1 (MQ=255)

                                                                                           atatCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaaa                                          <  2:2386424/134‑1 (MQ=255)

                                                                                           agatCAATTAACTAATGAAGATTATAATTTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTACAGGGATTTTCAAATGTTCATCAGTTGGATATaaaaaaa                                          <  2:1437780/132‑1 (MQ=14)

                                                                                            tatCAATTAACTAATGATGATTATAATGTAAAATAATTACGTAAAAGATATGACATACCAATAAATAAAGCGCCGAAGCTGTTGTTGAAAGGGACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAt                                         <  1:703468/134‑1 (MQ=255)

                                                                                             atCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAAtt                                        <  2:2133911/134‑1 (MQ=255)

                                                                                              tCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTg                                       >  2:1847009/1‑134 (MQ=255)

                                                                                               cAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa                                      >  2:1945182/1‑134 (MQ=255)

                                                                                               cAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGa                                      >  1:1771331/1‑134 (MQ=255)

                                                                                                aaTTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGAGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGaa                                     >  2:1489040/1‑134 (MQ=255)

                                                                                                   tAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAAttt                                  <  2:1553680/134‑1 (MQ=255)

                                                                                                     aCTAATGATGATTATAATGTAAAATAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                <  1:2817077/134‑1 (MQ=255)

                                                                                                     aCTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTCATAATTAATGGGATTGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTAc                                <  2:1174329/134‑1 (MQ=14)

                                                                                                      cTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGTTACAGGGAATTTAAAAAGTTCATCAGTTGAATATAAAAAAATTGAATTTACt                               >  1:488488/1‑134 (MQ=255)

                                                                                                      cTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGATGGATATAAAAAAATTGAATTTACt                               >  1:1112108/1‑134 (MQ=255)

                                                                                                             tgatTATAATGTAAAACAATTACGTAAAAGATATGACATACAAACAAATAAAACGCCGAAGCTGTTGTAGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACtttttagg                        >  2:1554480/1‑131 (MQ=255)

                                                                                                               atTATAATGTAAAACAATTACGTAAAAGATATGACATATCAACAAATAAAGTGTTGAAGTTGTTGTTGAAAGGTACAGGGAATTGAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                      <  1:1113645/134‑1 (MQ=17)

                                                                                                               atTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                      <  1:60135/134‑1 (MQ=255)

                                                                                                               atTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTagag                      <  1:1843771/134‑1 (MQ=255)

                                                                                                                 tataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGaa                    >  2:1689805/1‑134 (MQ=255)

                                                                                                                 tataATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATATACTTTTTTAGAGaa                    >  1:2544923/1‑134 (MQ=255)

                                                                                                                    aaTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaa                 >  1:95585/1‑134 (MQ=255)

                                                                                                                     aTGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaa                >  2:779657/1‑134 (MQ=255)

                                                                                                                      tGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGATTAATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaa               >  1:2221219/1‑134 (MQ=255)

                                                                                                                       gTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAAAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATaaaaa              >  1:67483/1‑134 (MQ=255)

                                                                                                                           aaCAATTAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGaa          <  2:1589604/134‑1 (MQ=255)

                                                                                                                           aaCAAGTACGTAAAATATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAACAATGaa          <  2:2525693/134‑1 (MQ=255)

                                                                                                                               aTTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGATACAGGGAATTTAAAAGGTTCATCAGATGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAata      >  2:887271/1‑134 (MQ=255)

                                                                                                                                ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAatat     <  1:767371/134‑1 (MQ=255)

                                                                                                                                ttACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGGTGAATATAAAAAAATTGAATTTACTTTTTTAGAGAATGAAAATGAAAatat     >  2:1387646/1‑134 (MQ=255)

                                                                                                                                 tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAATCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt    >  1:1226283/1‑134 (MQ=255)

                                                                                                                                 tACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTAATGAAAGATACAGGGAATATAAAAAGATCATAAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAtt    >  1:1203690/1‑134 (MQ=18)

                                                                                                                                  aCGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAAATGCTTTTCAAAGCTACAGGGAATATAAAAGGTTCATCAGTATGATATAAAAAAATTAACTTTACATTTTTAGAGAAtaaaaataaaaaatttt   >  2:1229221/1‑129 (MQ=17)

                                                                                                                                   cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGTGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta  <  1:1078575/134‑1 (MQ=255)

                                                                                                                                   cGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATAttta  >  2:1132191/1‑134 (MQ=255)

TTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAAATTGAATTTACTTTTTTAGAGAATAAAAATGAAAATATTTA  >  USA300TCH1516_ALE/111427‑111691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`