New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 800928	44 (1.090)	6 (0.160)	6/246	NT	12.4%	intergenic (+62/‑289)	hisC_1/USA300HOU_RS03915	Histidinol‑phosphate aminotransferase/Putative 5'(3')‑deoxyribonucleotidase
	?	USA300TCH1516_ALE	801948 =	43 (1.120)					intergenic (+189/+435)	USA300HOU_RS03915/USA300HOU_RS03920	Putative 5'(3')‑deoxyribonucleotidase/Putative lipid kinase

TGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTTGTAGA  >  USA300TCH1516_ALE/800788‑801062                                                                                                                                             |                                                                                                                                       tGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTATATCAAACTTTAAATACGAATAGTAAGTGGGAAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAAC                                                                                                                                        >  2:191478‑M1/1‑141 (MQ=255)    tgtTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACttt                                                                                                                                     >  2:338180/1‑140 (MQ=255)    tgtTAGAATTACAATTGATTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTg                                                                                                                                     <  2:240127/141‑1 (MQ=255)        aGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCACACCCCAACTTGCAtt                                                                                                                                 >  2:287166/1‑141 (MQ=255)          aaTTAGAATTGGTTTTAAAGAACAAAATGATAAAATGGTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACataaagaaa                                                                                                                               <  2:67169‑M1/141‑10 (MQ=255)             tACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTg                                                                                                                            <  2:413230/141‑1 (MQ=255)             tACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTg                                                                                                                            <  2:417371/141‑1 (MQ=255)               cAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACataaagaaatactt                                                                                                                          <  1:435006‑M1/141‑15 (MQ=255)                  ttGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGaa                                                                                                                       >  1:35042/1‑141 (MQ=255)                    ggTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTAGTCCAACCCCAACTAACATGATCTGTAGAAAt                                                                                                                     >  1:423846/1‑141 (MQ=37)                           aaGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAAt                                                                                                              >  1:379993/1‑141 (MQ=255)                             gAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATcc                                                                                                            <  1:291333/141‑1 (MQ=255)                              aaCAAAATGATAAAAGGGTAGAAGTTTTATCAAACTTTAAAGACGAAGAGTGAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATATATTTCGTCGTCCCACCCCGACTTGCATTGTCTGTAGAAATTGGGAATCCa                                                                                                           <  2:204558/141‑1 (MQ=21)                                 aaaaTGATAAAAGGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCaaat                                                                                                        <  2:208195/141‑3 (MQ=255)                                  aaaTGATAAAATGTTAGAAGTTTTATCAAACTTTAAATAAGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAAttt                                                                                                       <  1:257715/141‑1 (MQ=255)                                    aTGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTATAAATGGGGAATCCAATTtcc                                                                                                     >  2:320954/1‑140 (MQ=255)                                    aTGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCACTTtct                                                                                                     >  2:186649/1‑141 (MQ=255)                                     tGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTtctc                                                                                                    <  1:482849/141‑1 (MQ=255)                                              gTTAGGAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTggg                                                                                           <  2:230403/141‑1 (MQ=255)                                               ttAGAAGTTTTAGCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACataaagaaatactttttctttagaaattagtatttcttatgcatga                                                                                          <  2:39092‑M1/141‑47 (MQ=255)                                                 aGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGcc                                                                                        >  1:354083/1‑141 (MQ=255)                                                   aaGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGcccc                                                                                      >  1:327396/1‑141 (MQ=255)                                                       tttATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCccgccg                                                                                  >  2:431165/1‑141 (MQ=255)                                                         tagcAAAGTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGc                                                                                <  2:74255/138‑1 (MQ=255)                                                          aTCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTATATGTAGAAATTGGGAATCCACTTTCTCTTCGTTGGGGCCCCGCCggaa                                                                               >  2:263243/1‑139 (MQ=255)                                                          aTCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAAGGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACCTGCCTTGTCTGTAGACATTGGGAATCCAATTTCTCTTTGTTGGTTCCccacccccc                                                                               >  1:221230/1‑137 (MQ=17)                                                              aaCTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGCGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGt                                                                           <  2:394512/141‑1 (MQ=255)                                                                   tAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACataaagaaatactttttctttagaaattagtatttcttatgcatgagtgtaactcatgcattcata                                                                      <  2:320833‑M1/141‑67 (MQ=255)                                                                     aaTACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACataaagaaatactttttctttagaaattagtatttcttatgcatgagtgtaactcatgcattcatatt                                                                    <  1:291994‑M1/141‑69 (MQ=255)                                                                        aCGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACataaagaaatactttttctttagaaattagtatttcttatgcatgagtgtaactcatgcattcatattttt                                                                 <  1:267074‑M1/141‑72 (MQ=255)                                                                        aCGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAAt                                                                 <  1:397113/141‑1 (MQ=255)                                                                         cGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAAtt                                                                <  1:450923/141‑1 (MQ=255)                                                                               gTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGaaaaa                                                          >  1:300228/1‑141 (MQ=255)                                                                               gTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGaaaaa                                                          >  2:216123/1‑141 (MQ=255)                                                                                  aGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGc                                                       >  1:388638/1‑141 (MQ=255)                                                                                        gAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTa                                                 >  2:121088/1‑141 (MQ=38)                                                                                            gggACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGCAACTGGGTCGTTTTTTTTTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAg                                             >  1:365047/1‑141 (MQ=14)                                                                                            gggACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCTAATTTTTCTTTGTTGGGGCCCCGCCAACAAAGATGACTAGAATTGAAAAAAGCTTGTTACAAg                                             >  1:105168/1‑141 (MQ=14)                                                                                                cAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCa                                         >  2:303867/1‑141 (MQ=40)                                                                                                cAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCa                                         <  2:327396/141‑1 (MQ=39)                                                                                                       gATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGt                                  <  1:415078/141‑1 (MQ=40)                                                                                                          aCTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCa                               <  1:121088/141‑1 (MQ=38)                                                                                                            tttCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGt                             <  1:431165/141‑1 (MQ=37)                                                                                                             ttCGCAAAATTTATTTCGTCGTCCCACCTCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTc                            <  2:20522/141‑1 (MQ=25)                                                                                                                gCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAac                         <  1:225212/141‑1 (MQ=34)                                                                                                                gCAAAATTTATTTCGTCGTCCCACACCTTCTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAac                         <  2:325733/141‑1 (MQ=255)                                                                                                                   aaaTTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAactac                      <  2:341009/141‑1 (MQ=32)                                                                                                                       tgatttCCTCGTCCCCCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGcc                  <  1:438343/139‑1 (MQ=12)                                                                                                                           ttCGTCGTCCCACTCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAata              <  2:97250/141‑1 (MQ=25)                                                                                                                            tcgtccTCCCGTCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAatat             <  1:90855/141‑1 (MQ=255)                                                                                                                               tcgtcCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAAc          <  1:372410/141‑1 (MQ=35)                                                                                                                                 gtcCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACtt        <  2:354083/141‑1 (MQ=35)                                                                                                                                       ccccAACCTGCATTGTCTGCTGATATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTTGTaga  <  2:221946/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       TGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTTGTAGA  >  USA300TCH1516_ALE/800788‑801062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.