breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000731 | 81 | N→G | 100% | intergenic (–/‑151) | – / → repA | –/replication protein A |
JC | CP000731 | 6,552 | +28 bp | 100% | intergenic (+1069/‑419) | cadX → / → USA300HOU_pUSA300HOUMR0010 | cadmium efflux regulator/MarR family transcriptional regulator |
RA | CP000731 | 25,663 | Δ1 bp | 100% | coding (316/333 nt) | USA300HOU_pUSA300HOUMR0031 → | hypothetical protein |
RA | USA300TCH1516_ALE | 110,651 | C→T | 11.6% | D170D (GAC→GAT) | USA300HOU_RS00515 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 110,660 | T→A | 16.5% | I173I (ATT→ATA) | USA300HOU_RS00515 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 111,512 | A→G | 36.0% | S194S (TCA→TCG) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 111,516 | C→A | 37.7% | Q196K (CAA→AAA) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 111,560 | T→C | 9.9% | R210R (CGT→CGC) | USA300HOU_RS00520 → | putative lipoprotein |
RA | USA300TCH1516_ALE | 512,643 | A→G | 48.1% | intergenic (+711/‑6052) | recR → / → speA_2 | Recombination protein RecR/Arginine decarboxylase |
RA | USA300TCH1516_ALE | 589,875 | C→A | 8.0% | P31T (CCT→ACT) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
RA | USA300TCH1516_ALE | 1,138,882 | T→C | 6.5% | K203K (AAA→AAG) | isdA ← | Iron‑regulated surface determinant protein A |
RA | USA300TCH1516_ALE | 1,416,458 | A→T | 100% | I216N (ATT→AAT) | oppD_4 ← | Oligopeptide transport ATP‑binding protein OppD |
RA | USA300TCH1516_ALE | 1,513,930 | A→T | 100% | L413F (TTA→TTT) | USA300HOU_RS07375 → | hypothetical protein |
RA | USA300TCH1516_ALE | 1,646,189 | G→C | 100% | A155G (GCA→GGA) | ypdF ← | Aminopeptidase YpdF |
RA | USA300TCH1516_ALE | 1,744,607 | T→C | 100% | D22G (GAT→GGT) | apt ← | Adenine phosphoribosyltransferase |
RA | USA300TCH1516_ALE | 2,010,363 | C→T | 100% | C146Y (TGT→TAT) | USA300HOU_RS10125 ← | Putative multidrug export ATP‑binding/permease protein |
RA | USA300TCH1516_ALE | 2,028,191 | C→A | 72.7% | A152S (GCA→TCA) | USA300HOU_RS10230 ← | hypothetical protein |
RA | USA300TCH1516_ALE | 2,279,705 | G→A | 100% | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
RA | USA300TCH1516_ALE | 2,532,298 | A→T | 100% | T15T (ACA→ACT) | femA_3 → | Aminoacyltransferase FemA |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | USA300TCH1516_ALE | 2176683–2181135 | 2181135 | 1–4453 | 14 [13] | [13] 14 | ilvA/USA300TCH1516_02069 | L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | USA300TCH1516_ALE | 512620 = | 45 (1.110) | 33 (1.250) +45 bp |
26/170 | NT | 51.6% | intergenic (+688/‑6075) | recR/speA_2 | Recombination protein RecR/Arginine decarboxylase |
? | USA300TCH1516_ALE | 2176447 = | 50 (1.230) | intergenic (+209/+5086) | ilvA/USA300TCH1516_02069 | L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly | |||||
* | ? | USA300TCH1516_ALE | = 800928 | 44 (1.090) | 6 (0.160) | 6/246 | NT | 12.4% | intergenic (+62/‑289) | hisC_1/USA300HOU_RS03915 | Histidinol‑phosphate aminotransferase/Putative 5'(3')‑deoxyribonucleotidase |
? | USA300TCH1516_ALE | 801948 = | 43 (1.120) | intergenic (+189/+435) | USA300HOU_RS03915/USA300HOU_RS03920 | Putative 5'(3')‑deoxyribonucleotidase/Putative lipid kinase | |||||
* | ? | USA300TCH1516_ALE | 895785 = | 46 (1.140) | 4 (0.110) | 4/226 | NT | 11.3% | coding (806/822 nt) | metQ_2 | Methionine‑binding lipoprotein MetQ |
? | USA300TCH1516_ALE | = 909740 | 23 (0.650) | intergenic (‑663/‑371) | USA300HOU_RS04500/csbD_1 | hypothetical protein/Stress response protein CsbD | |||||
* | ? | USA300TCH1516_ALE | 2085296 = | 37 (0.910) | 6 (0.170) | 5/232 | NT | 17.4% | coding (173/201 nt) | hlb_1 | Phospholipase C |
? | USA300TCH1516_ALE | = 2128343 | 24 (0.660) | coding (1/825 nt) | hlb_2 | Phospholipase C |