Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,279,705 G→A 100% A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,279,7050GA99.6% 97.5 / ‑6.9 38A943V (GCA→GTA) ebh_3Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (20/17);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279568‑2279841
                                                                                                                                         |                                                                                                                                        
ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                       >  1:262451/1‑141 (MQ=255)
    gCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttgt                                                                                                                                   >  2:163200/1‑141 (MQ=255)
     ccGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGACTTGTTATTAGCTTGGGGGTTATCATTTGCATCATCCGTTGCTAATGTTACTgttgtt                                                                                                                                  >  2:239864/1‑141 (MQ=255)
     ccGATAGTAGTTGATAGTACTGCTGGTTTACTAGTTACATCGTTATCCATTGACGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt                                                                                                                                  <  1:54072/141‑1 (MQ=255)
       gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGGTGATGTTACTGTTGTTTc                                                                                                                                <  1:295941/141‑1 (MQ=255)
           ttaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCt                                                                                                                            <  2:63443/141‑1 (MQ=255)
            taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTCTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTGAcccg                                                                                                                           >  2:166426/1‑139 (MQ=255)
               ttGTTTCTACGTCTGGTTTACTAGTTGCATTGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg                                                                                                                        <  2:179550/141‑1 (MQ=255)
                   ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                    >  1:273780/1‑141 (MQ=255)
                      tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                                 >  2:245719/1‑141 (MQ=255)
                        cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc                                                                                                               >  1:20947/1‑141 (MQ=255)
                         ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt                                                                                                              <  1:29601/141‑1 (MQ=255)
                          tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGTCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGATTTTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTGCACCTGTTGTCGCATCACtt                                                                                                             >  1:261906/1‑140 (MQ=255)
                           cTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat                                                                                                            >  2:82717/1‑141 (MQ=255)
                             ggTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatta                                                                                                          <  2:242031/141‑1 (MQ=255)
                                             cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                          >  1:139255/1‑141 (MQ=255)
                                                   tcATTTTTGGTCTTTTTGTTGTTGTTTTTACACTAGAATTGTTATTATCTTGCTGTTTATCATTTGCATCATCAGTTGCTTATGTTACTGTTGTTTCTGTTGTTGTCGCATCACTATTATTTGGTGTTGTCGGAGAAgtgt                                                                                    <  2:247946/140‑1 (MQ=255)
                                                              cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                         >  1:181563/1‑141 (MQ=255)
                                                                        attcATTTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTGTCGACTCTTGCCGCATCACTATTATTTGGTGTTGTCGGTGCAGCGGTGGTTTAGCCATTAGCTgtcg                                                               <  2:38689/138‑1 (MQ=255)
                                                                             tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa                                                          <  2:262451/141‑1 (MQ=255)
                                                                                acacTAGAATTGTTATTCGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTCATTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCCTTAGCTGTCGTCTCAGAt                                                       >  2:79669/1‑141 (MQ=255)
                                                                                   cTAGAATTGTTATTAGCTTGCGGTTTCCCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAAGAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACg                                                    >  2:68148/1‑141 (MQ=255)
                                                                                     aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCAGAGAAGCGTCTGCTTTTCCCTTAGCTGTCGTCTCAGATACGtt                                                  >  2:90522/1‑141 (MQ=255)
                                                                                      gAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTAAGATACGTTa                                                 >  1:200329/1‑141 (MQ=255)
                                                                                             tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                          >  1:65285/1‑141 (MQ=255)
                                                                                                 aGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGt                                      <  1:163200/141‑1 (MQ=255)
                                                                                                  gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGGTGTCCGAGAAGCGTCTGTTTTGCCTTTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTa                                     <  1:180614/141‑1 (MQ=255)
                                                                                                     tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt                                  >  1:309111/1‑141 (MQ=255)
                                                                                                       gggTTTATTCGTTGTATCATCAGTTGGTGATGTTATTGTTGTTTCACCTGTTGCCGTGTCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  2:31569/140‑1 (MQ=255)
                                                                                                       ctgTTTATCATTTGCATCCTCAGTTGCGGATGTTACTGTTGTTTCACCGGTTGCCGCATCACTATTATTTGGTGTGGGCGGAGATGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  1:23943/139‑1 (MQ=255)
                                                                                                       cGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGGCGGAGACGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  2:261906/141‑1 (MQ=255)
                                                                                                         gTTTATGTTTTACAGCACCAGTTGCTGTTGGTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                              <  1:70560/141‑1 (MQ=255)
                                                                                                             aTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGCTTGTCTAGTATTTTCTGgtgt                          >  2:34401/1‑141 (MQ=255)
                                                                                                                    gCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTa                   <  1:245719/141‑1 (MQ=255)
                                                                                                                          tcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGTTGTCCCGTATTTTCTGGTGTTGCATTAGCAttt             >  2:241630/1‑141 (MQ=255)
                                                                                                                                  tGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:16836/141‑1 (MQ=255)
                                                                                                                                    aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATGTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg   <  1:271969/141‑1 (MQ=255)
                                                                                                                                     tGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGGTTTGCCATTAGCTGTCGTCTCAGATACGTGAGGTTGTCCAGTATTTTCTGGTGTTGGATTAGCATTTGAATTTGCTGt  >  1:164954/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  USA300TCH1516_ALE/2279568‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: