Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 614,094 T→C 5.5% S802S (AGT→AGC sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE614,0940TC 5.5% 969.3 / 6.6 427S802S (AGT→AGCsdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base T (184/192);  new base C (24/7);  total (222/205)
Fisher's exact test for biased strand distribution p-value = 2.47e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 431 total aligned reads displayed.

TCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGA  >  USA300TCH1516_ALE/613954‑614234
                                                                                                                                            |                                                                                                                                            
taagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTAGGATAGCGACTCAGACTAAGATAGCAACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGt                                                                                                                                              <  1:616814/139‑1 (MQ=255)
gaagattcagaAAGCGACGGAGACTGAGACAGCGATTACGACTCGGATAGCGACCCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGt                                                                                                                                              <  2:3190361/139‑1 (MQ=17)
 cagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                                                                                                                             >  1:3960752/1‑141 (MQ=255)
 cagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                                                                                                                             >  1:4238335/1‑141 (MQ=255)
  agattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGCACAACCTAGCGAGTCAGATTCAGATAGCGATTCGGACTCAGACAGCga                                                                                                                                            >  1:4157261/1‑141 (MQ=17)
  agattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTga                                                                                                                                            >  1:3731543/1‑141 (MQ=255)
        agaCATGGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGTCTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcaga                                                                                                                                      <  2:2926063/141‑1 (MQ=255)
        agaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcaga                                                                                                                                      <  1:3685850/141‑1 (MQ=255)
         gaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagat                                                                                                                                     <  1:2493263/141‑1 (MQ=255)
           cAGCGACTTAGACTCAGACAGCGATTCAGACTCTGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                                                                                                                                   <  2:1660426/141‑1 (MQ=255)
           cAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGAATCAGATAGCCACTCCGACTCAGACAAAgattcagacac                                                                                                                                   >  2:499042/1‑138 (MQ=17)
            aGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTGAGAGAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattca                                                                                                                                  >  1:4364705/1‑141 (MQ=255)
            aGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACCCAGTGTCAAATATCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattca                                                                                                                                  >  1:3222295/1‑141 (MQ=18)
            aGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATGCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattca                                                                                                                                  >  1:1375571/1‑141 (MQ=255)
               cactcagacgcagACAGCGATTCAGACTCGGATAACGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCAACTCCGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGAc                                                                                                                               <  1:1997047/140‑1 (MQ=255)
                 ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTc                                                                                                                             >  1:2028599/1‑141 (MQ=255)
                 ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTc                                                                                                                             <  1:946701/141‑1 (MQ=255)
                    agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACTCAGa                                                                                                                          >  1:3096870/1‑141 (MQ=255)
                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGAt                                                                                                                         >  1:3319852/1‑141 (MQ=255)
                       tgcagacAGCGATTCAGAGTCGGATAGCGCCTGAGACTCGGATACAGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAg                                                                                                                       <  2:1066516/139‑1 (MQ=17)
                       ctcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAg                                                                                                                       >  1:1116349/1‑141 (MQ=255)
                       ctcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAg                                                                                                                       <  1:375564/141‑1 (MQ=255)
                          agacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCGGACTCAGATAGCGa                                                                                                                    <  1:3580826/141‑1 (MQ=255)
                           gagatagATTAAGACGGGGACAGCGACGCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                                                                                                   <  2:2791574/135‑1 (MQ=17)
                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                                                                                                   >  1:296279/1‑141 (MQ=255)
                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATACGGACCCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                                                                                                   >  2:4001803/1‑141 (MQ=255)
                            acAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACt                                                                                                                  <  1:1493550/141‑1 (MQ=255)
                                 gATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGAt                                                                                                             <  1:3816326/141‑1 (MQ=255)
                                    tCAGACTCGGATAGCGTCGCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCt                                                                                                          >  1:4397487/1‑141 (MQ=255)
                                    tCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCt                                                                                                          >  1:3410119/1‑141 (MQ=255)
                                    tCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCCGACTCAGACAGCGACTCAGATTCAGACaccgatccagactcagacgcc                                                                                                          >  2:3010037‑M1/1‑120 (MQ=255)
                                      aGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGa                                                                                                        <  1:725384/141‑1 (MQ=255)
                                         cTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAg                                                                                                     <  1:2332293/141‑1 (MQ=255)
                                          tCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGATTCAGAGAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTAAGATTCAGACTCAGATACAGACTCAGATACTGACaca                                                                                                    >  2:749872/1‑139 (MQ=17)
                                           cGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCg                                                                                                   >  1:1807208/1‑141 (MQ=255)
                                           cGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCg                                                                                                   >  1:529837/1‑141 (MQ=255)
                                             gATAGCGGCGCAGACTCAGATATCGACTGAGATTGGGATTGCGACTCAGAACGACGCACCGACGAAGATGAAGATAGCGATATTGATACAGACAGTGACGAAGATGCAGAATCAGATAGCGACTCAGATTCTGACAGCGAt                                                                                                 <  2:1224109/141‑1 (MQ=255)
                                                 gCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcag                                                                                             <  1:1869745/141‑1 (MQ=255)
                                                   gactcagactcaGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATCCGGACTCAGACAATGATTCAGAATCAGACTCAGATAGCGACTCAGAATATGACAACGACacagaa                                                                                           >  2:2046719/1‑140 (MQ=18)
                                                   gactcagactcaGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCCGCTTATTTCGCAGAGTCAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGAGATtcagac                                                                                           >  2:2002690/1‑141 (MQ=17)
                                                   gactcagactaaGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagac                                                                                           <  1:4007772/141‑1 (MQ=255)
                                                      tcagactcagaTAGCGACTCAAATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactca                                                                                        <  1:1222526/141‑1 (MQ=255)
                                                       cagactcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactcag                                                                                       >  1:3364509/1‑141 (MQ=255)
                                                           ctcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAg                                                                                   <  1:4069929/141‑1 (MQ=255)
                                                            tcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGc                                                                                  >  1:573397/1‑141 (MQ=255)
                                                             cagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCg                                                                                 <  1:3632616/141‑1 (MQ=255)
                                                              agaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGCCTCTCACGGTGGTTTGGAGTCAGATTCAGACAGCGATTCAGACTCAGACAGCga                                                                                >  1:1008499/1‑141 (MQ=17)
                                                                 tAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGGTTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCGGATTCTGACAGCGATTCAAACTCAGACAGCgactc                                                                             >  1:747970/1‑141 (MQ=255)
                                                                   gtcaCTCAGATTCGGATAGCGATTCAGACTTAGAGAGAGATTCAGATTCAGATCGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcag                                                                           <  2:3680562/138‑1 (MQ=21)
                                                                   aggACTAAGAGTCTGATAGCGAGGCAGACGCAGATAGCGATGCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGAAGCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcag                                                                           <  2:2588677/139‑1 (MQ=21)
                                                                    cGATTCCGATTGCGACAGCGATTCAGACTGAGATAGCGATTCAGATTAAGATAGCGATTCGGACTCAGACTGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcaga                                                                          <  2:1854310/141‑1 (MQ=18)
                                                                    cGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACACAGATTTAGACTCAGGCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcagc                                                                          >  1:392361/1‑140 (MQ=21)
                                                                       cTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcagactc                                                                       <  1:1557204/141‑1 (MQ=255)
                                                                       cTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcagactc                                                                       >  1:2553055/1‑141 (MQ=255)
                                                                       cTCAGATTCGGACAGCGAATCAGATTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcagactc                                                                       <  1:1654172/141‑1 (MQ=255)
                                                                        tCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGCCTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGACTCAGACTCAGCCAGCgactcagcggaa                                                                      >  1:2048387/1‑136 (MQ=255)
                                                                        tCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGGGGTTCAGTCTGATACAGATATCGCGACTCAGATTCTGATAGCGAATAAGACTCCGACAACgactcagaatca                                                                      >  2:944224/1‑141 (MQ=17)
                                                                         cAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGagtcagactcag                                                                     >  1:2107269/1‑141 (MQ=255)
                                                                          aGATTGGGATTGAGACTCAGACTGAGAGAGTGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGActcagactcaga                                                                    <  2:4119606/141‑1 (MQ=21)
                                                                          aGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTCAGACACAGATTCAGACTCAGATACTGACACCCATTCAGACCCCGACACCaactcacacaccga                                                                    >  2:3411338/1‑136 (MQ=14)
                                                                            atgcGGATAGCGAATCAGACGCAGATACCGATTCAGATGCAGATTGCGATTCGGATTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACa                                                                  <  2:3955988/138‑1 (MQ=21)
                                                                             ttCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAg                                                                 <  1:348127/141‑1 (MQ=255)
                                                                               cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGAGAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTg                                                               >  1:2603850/1‑141 (MQ=255)
                                                                                 gATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgat                                                             >  1:3283561/1‑141 (MQ=255)
                                                                                    aGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattca                                                          >  1:1443930/1‑141 (MQ=255)
                                                                                          ggagacgcagaGAGAGATGCAGATTCAGATTGCGATTCGGACTCAGACTGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattcagattca                                                    <  2:140102/139‑1 (MQ=21)
                                                                                              actcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACa                                                >  1:2169997/1‑141 (MQ=255)
                                                                                                tcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGc                                              <  1:2916705/141‑1 (MQ=255)
                                                                                                gcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGc                                              <  1:2840795/140‑1 (MQ=255)
                                                                                                 cagaTAGCGATTCAGCTTCGGATAGGGATTCGGGCTCAGACAGAGATTCAGGTTTAGACTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCACACAGTGATTCAGATTCAGACAGCg                                             <  2:4357881/141‑1 (MQ=18)
                                                                                                  agaTAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATACTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGAGATCCAGACACAGACAGcca                                            >  2:3722291/1‑139 (MQ=18)
                                                                                                   gagatgtattcagattgtgACTGCGATACGGAGTAAGACAGTGAATCAGATGCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGAc                                           <  2:2384665/134‑1 (MQ=17)
                                                                                                    attgtgattctgatagaGATAGAGAATTGGACACAGATTGTGATTGAGATTGAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACt                                          <  2:294506/136‑1 (MQ=14)
                                                                                                     tAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtc                                         <  1:3869492/141‑1 (MQ=255)
                                                                                                       gctattcagttttagTTAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcag                                       <  2:3597139/138‑1 (MQ=18)
                                                                                                        cGATTCAGATTCAGATAGCGATTCGGACTAAGACAGTGATTCAGATTCAGACTCAGATAACGACTCAGATTATGATAGCGATTTAGACTTAGACAGCGACTCAGATTCAGATAGTGAAACAGATTCAGAAAGTGATttaga                                      >  1:803774/1‑141 (MQ=18)
                                                                                                         gattcagattcaGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCACACTCAGACAGCGACTCAGACACAGACAGTGATTCAAACTCAGACAACGCCtcacaa                                     >  2:600862/1‑138 (MQ=21)
                                                                                                         acgccagatccagagGACGATGCTGACTCAGGCAGAGATTCAGATGCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                     <  2:824079/137‑1 (MQ=17)
                                                                                                            tcagattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTCACTCCGCTGCACTCAGCGACtcagcttca                                  >  1:2760821/1‑141 (MQ=21)
                                                                                                            atagattcagatAGAGAGTGAGTCTCACAATGTGTTACAGTTTCAGACTCAGAGAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattca                                  <  2:4238335/139‑1 (MQ=14)
                                                                                                               gattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGCATCCGATATTGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcagat                               >  1:3029383/1‑141 (MQ=21)
                                                                                                               gattcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcagat                               <  1:681047/141‑1 (MQ=255)
                                                                                                                attcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGAGTCCGATAGCGACTCAGATTCAGACAACGATACAGACTCAAACAGCAACACAGACGCAGAAAGTGATTCAGATACAGATAGCGAAtcagcttcacaca                              >  2:3314443/1‑137 (MQ=18)
                                                                                                                attcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGAGAGTCGGGTTGGGACCGCGATTGAGACGCAGACAGCGACTCAGAGTCAGACAGTGATTAAGATTCAGACAACGACTCAGCTtcagata                              >  2:2189412/1‑141 (MQ=21)
                                                                                                                 tacagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatag                             <  1:3164923/139‑1 (MQ=255)
                                                                                                                   cagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcg                           >  1:2242375/1‑141 (MQ=255)
                                                                                                                     gatAGCGATGCAGACTCAGATAGTGATTCAGAGTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                         <  2:2503233/141‑1 (MQ=255)
                                                                                                                      atAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGAGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGCCTCAGACTCAGACAGTGAGCCAGATTCAGCCAGCGACTTACATtcacaatgcgact                        >  2:1488416/1‑141 (MQ=18)
                                                                                                                       ttgCGATTTGGCCTCATACAGCGATCCAGATTCAGACTCAGATATCGATGCAGATTCGGCTACCGACGCAGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGATTCAGACAGCGCCTCAGATtcagatagcgactc                       <  2:3865354/139‑1 (MQ=14)
                                                                                                                         gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTGAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatatttactcag                     >  2:3493461/1‑141 (MQ=255)
                                                                                                                         gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTGAGACAGCGACTCAGATtcagatagcgactcag                     >  1:140519/1‑141 (MQ=255)
                                                                                                                         gCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATACAGACTCAGACACCGACTCAGCCTCAGACAGTGAGTCACATTCAGCCAGAGACTCAGATccagataccgaaacag                     >  2:4199806/1‑136 (MQ=18)
                                                                                                                          cGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGTCTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcaga                    >  1:4298340/1‑141 (MQ=255)
                                                                                                                              tCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactca                >  1:1201131/1‑141 (MQ=255)
                                                                                                                                 gACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcggctcaggctcagaa             >  1:875698/1‑140 (MQ=255)
                                                                                                                                   cTCAGACAGTGATACAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatag           <  1:3529545/141‑1 (MQ=255)
                                                                                                                                         cAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactc     <  1:2702010/141‑1 (MQ=255)
                                                                                                                                         cAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTAAGACTCAGACAGCGACTCCGACGCAGACAGTGATTCAGATGCAGACAGCGACTGAGGTtcagatagcgactcagactcagatagcgactt     >  1:1939871/1‑140 (MQ=17)
                                                                                                                                         cAGTGATTCAGATTCAGACTCAGAGAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactc     <  1:1763586/141‑1 (MQ=255)
                                                                                                                                            tGATTCAGAGTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcaga  <  1:2656976/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGA  >  USA300TCH1516_ALE/613954‑614234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: