Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,387 T→C 6.0% D1163D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,3870TC 6.0% 1046.5 / 6.4 414D1163D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (192/177);  new base C (25/9);  total (220/194)
Fisher's exact test for biased strand distribution p-value = 1.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 442 total aligned reads displayed.

GGATACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTC  >  USA300TCH1516_ALE/618247‑618527
                                                                                                                                            |                                                                                                                                            
ggATACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCgat                                                                                                                                              <  1:2377376/141‑1 (MQ=255)
ggATACTACGAAGAACAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCgat                                                                                                                                              <  1:3546710/141‑1 (MQ=255)
ggATACGACGAAGAAGAAACATCGGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCgat                                                                                                                                              <  2:1101868/141‑1 (MQ=255)
gaaTACGACGAAGAAGAAACATCAGATAGCGACTCAGATGCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGAGAGCGATTCAGATTCAGATAGCgat                                                                                                                                              <  2:1828084/139‑1 (MQ=255)
  aTACTACGAATAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCgattc                                                                                                                                            <  1:3264020/141‑1 (MQ=255)
   tactaaGAAGGAGGAACATCAGAGAGGGTCTAGGATGCTGACAGGGGCTCAGATTGAGACAGAGAGTCAGACTCAGATAGGGATTCAGACTCGGATAGCGATTAAGCTACAGCGTATGTACCAGATGCAGATAGCgattca                                                                                                                                           <  2:327368/141‑1 (MQ=255)
    actacGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAg                                                                                                                                          >  1:105661‑M2/1‑140 (MQ=255)
       acGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAgatt                                                                                                                                       <  1:3847839‑M2/141‑5 (MQ=255)
       acGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAATCAGAATCAGATAGCGACTCAgatt                                                                                                                                       <  2:3484620‑M2/141‑5 (MQ=255)
         gaagaagaaACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAgattca                                                                                                                                     >  1:547456‑M2/1‑135 (MQ=255)
          aagaagaaACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAgattcag                                                                                                                                    >  1:3987813‑M2/1‑134 (MQ=255)
            gaagaaACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAgattcagac                                                                                                                                  >  2:3091883‑M2/1‑132 (MQ=255)
               gaaACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGATTCAGACTCAAACAGCGCCTCAGATTCAGATAGCGATTCAGATTcatacaga                                                                                                                               >  2:4394233/1‑141 (MQ=14)
               gaaACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTcagacaga                                                                                                                               >  1:1960622/1‑141 (MQ=255)
               gaaACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTcagacaga                                                                                                                               <  1:1451/141‑1 (MQ=255)
                aaaCATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAgagtcagacagcg                                                                                                                              <  2:3670590‑M2/141‑14 (MQ=255)
                   caacAGATAGCGACTCAGATTCTGACAGCGACTAAGATTCAGACAGAGACTCAGACGCAGATAGTGATACAGACTCGGATAGAGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACt                                                                                                                           <  1:1818392/138‑1 (MQ=18)
                    aTCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGAGACTc                                                                                                                          >  2:11835/1‑141 (MQ=11)
                     tCAGATATCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCa                                                                                                                         <  2:2572968/141‑1 (MQ=255)
                     tCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCa                                                                                                                         <  1:2669536/141‑1 (MQ=255)
                       aGATAGCGACTCAGATTCGGGCAGCGACTCAGATTCAGGCAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGa                                                                                                                       <  1:963761/141‑1 (MQ=255)
                        gATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGATAGAGACTCAGAt                                                                                                                      >  2:2264117/1‑141 (MQ=255)
                           aGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGt                                                                                                                   <  2:1035441/141‑1 (MQ=255)
                            gCGTCTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTg                                                                                                                  <  1:2502774/141‑1 (MQ=255)
                             cGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGa                                                                                                                 >  1:2774217/1‑141 (MQ=255)
                             cGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATACTGa                                                                                                                 >  1:4225778/1‑141 (MQ=255)
                                cTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGACTCAGAGAGAGAATCACATTGTGagac                                                                                                              >  2:3615634/1‑138 (MQ=14)
                                   aGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGa                                                                                                           >  1:3072499/1‑141 (MQ=255)
                                    gATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGAc                                                                                                          <  1:2698763/141‑1 (MQ=255)
                                       tCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCa                                                                                                       >  1:915175/1‑141 (MQ=255)
                                             aGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagc                                                                                                 >  1:301203/1‑141 (MQ=255)
                                              gCGACTCACATTCAGACAGAGACTCAGACCCAGATAGTGATTCAGACTCGGATAGCGATTCAGGTTCAGCCAGCGATTCAGAGTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcg                                                                                                <  1:2301011/141‑1 (MQ=21)
                                               ggACTTAGATTCAGACAGAGAGTCAGACTTAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcga                                                                                               <  2:3557407/140‑1 (MQ=255)
                                                gACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCCCTGCCTGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgac                                                                                              >  1:1022017/1‑141 (MQ=21)
                                                  cTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGCTAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATACAGACTCAGATagcgactc                                                                                            >  1:1373564/1‑141 (MQ=255)
                                                  cTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactc                                                                                            >  1:256780/1‑141 (MQ=255)
                                                     aGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGCTTCAGATTCCGCTAGCGATTCAGATTCAGACAGAGACTCCGCTAGTGATTCAGACTCAGATagcgactcaca                                                                                         >  1:1604211/1‑139 (MQ=21)
                                                      aaTTTAGACAGAGACTCAGAATCAAATATGGATTCAGACTAAGATAGAGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCTATTCAGATTCAGACAGAGACTCAGATAGTGATTCCGCCCAATAGagcgagtcagat                                                                                        <  2:554725/140‑1 (MQ=17)
                                                       aTTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagatt                                                                                       >  1:3127445/1‑141 (MQ=255)
                                                        cccagacagaTACTCAGCCTCAGATAGTGATTCAGACTCGGATAGCGATGCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGGGACTCAGATAGTGATTCAGACTCAGATagcgactcagattc                                                                                      <  1:1733669/139‑1 (MQ=255)
                                                            gacagaGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagac                                                                                  <  1:4121486/141‑1 (MQ=255)
                                                              cagaGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacag                                                                                <  1:3329330/141‑1 (MQ=255)
                                                               agagagTCAGACTCAGATAGTGATTCAGACTCAGATAGAGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACATAGACTCATATAGTGATTCAGACTCAGATagcgactcagattcagacagc                                                                               <  2:2339246/141‑1 (MQ=21)
                                                               agagACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagc                                                                               >  1:2741082/1‑141 (MQ=255)
                                                                gagaCTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacatcg                                                                              >  1:342546/1‑141 (MQ=255)
                                                                 agagtcagacttAGATAAGGATTCAGACCCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcga                                                                             <  2:4211662/141‑1 (MQ=21)
                                                                 agactcagactcAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagactcagacagcga                                                                             >  1:3349759/1‑141 (MQ=255)
                                                                   tctgattctcagaTAGTAATTCAGCCTCGGATAGCGATGCAGATGCAGACAGCGATTCAGATTCAGAGAGCGATTCAGAGGCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgcctcagattcagacagcgact                                                                           <  1:1245323/134‑1 (MQ=21)
                                                                    ctcagactcagaTAGTGATTCAGACTCGGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGAGACTCAGATAGTGATACAGACTCAGATagcgactgagattcagacagcgagcc                                                                          >  2:1458380/1‑138 (MQ=18)
                                                                     tcagactcagaTAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGACTACGGCCGGGACAGAGGCTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactca                                                                         >  1:3228994/1‑141 (MQ=18)
                                                                       agacgcagaTAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactcaga                                                                       <  1:2615849/141‑1 (MQ=255)
                                                                         actcagaTAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactcagctt                                                                     >  1:222160/1‑141 (MQ=255)
                                                                          ctcagaTAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactcagattc                                                                    <  1:2909166/141‑1 (MQ=255)
                                                                          atcagaTAGGGATTATAAGTCGGATAGAGATACAGATTCAGACAGAGATTCAGATTCAGATAGCGATTCAAATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactcagattc                                                                    <  2:2155871/140‑1 (MQ=18)
                                                                            gagaTAGGGATTCAGACTCGGATAGTGATTCAGATTCAGACAGAAATTCAGATGCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactcagattcag                                                                  <  2:774613/140‑1 (MQ=21)
                                                                            cagaTAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactccgattcag                                                                  >  1:185851/1‑141 (MQ=255)
                                                                             agaTAGAGATTAAGAAGGTGATAGAGATTGCGAATGAGACAGAGAATCAGAGACAGATAGTGATTCAGATTCAGATAGAGACTCAGATAGTGATTCAGACTCAGATagcgactcagattcagacagcgactcagattcaga                                                                 <  2:4139870/141‑1 (MQ=17)
                                                                              gaTAGTGATTCAGACTCGGATAGCGCCGCAGATGCAGACAGCGATTCAGATTCAGATAGCGATTCAGAGTCAGACAAATTCACAGATATTGATGCAGACTCAGATagcgacacagactcagacaccgacacagactcagac                                                                >  2:81573/1‑141 (MQ=18)
                                                                               aTAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATACAGATTCAGACTCAGATAgcgactgagattcagacagcgacacagatacagaca                                                               >  2:1296659/1‑141 (MQ=21)
                                                                                tAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGcgactcagattcagacagcgactcagattcagacag                                                              <  1:1997008/141‑1 (MQ=255)
                                                                                 aGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCgactcagattcagacagcgactcagattcagacagc                                                             <  1:3601604/141‑1 (MQ=255)
                                                                                 aGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCgactcagattcagacagcgactcagattcagacagc                                                             <  1:3940400/141‑1 (MQ=255)
                                                                                    aaTTCAGCCGCGGATGACGATTCAGATTCACACGGCGATCTAGATTTAGATAGCGATTCAGATTCAGACAGAGAATCAGATAGTGATGCAGACTCAGATAGCGACtcagattcagacagcgactcagattcagacagcgac                                                          <  2:1906585/140‑1 (MQ=18)
                                                                                      ttCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCagattcagacagcgactcagattcagacagcgactc                                                        >  1:4282851/1‑141 (MQ=255)
                                                                                      ttCAGACTCGGATAGCGATTCAGATTCAGACAGCGATACAGATTCAGATAGCGACTCAGATTCAGATAGAGACTCAGACACAGATTTAGACTCAGATTGCGACacagattcagacaccgaccgcgactcagacagcgacac                                                        >  2:2941711/1‑139 (MQ=17)
                                                                                      ttCAGACGCGGATAGTGGTTCGGATTCTGACGGAGATTTAAATGGAGATAGTGATTTAGATTCAGACAGAGACTGAGAGAGGGATGCAAACTCAGATAGCGACTCagattcagacagcgactcagattcagacagcgactc                                                        <  2:3841642/141‑1 (MQ=16)
                                                                                      tgcacaCTCCGATAGCGATTCAGATTCAAAAAGACACTCTGATGCTGATAGTGATTCAAATTCAGACAAAGAATAAGATGGTGATTAAGCATCAGATAGCGACTCagattcagacagcgactcagattcagacagcgactc                                                        <  2:4096682/136‑1 (MQ=12)
                                                                                        cAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCagattcagacagcgactcagattcagacagcgactccg                                                      >  1:1332429/1‑139 (MQ=255)
                                                                                         aGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTAAGAttcaggcagcgactcagattcagacagcgactcaga                                                     >  1:3000230/1‑141 (MQ=255)
                                                                                           aCTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCgactcagact                                                   >  1:1164136/1‑141 (MQ=255)
                                                                                            cTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCgactcagactc                                                  >  1:2853190/1‑141 (MQ=255)
                                                                                            cTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACACATTTTCAGACAGCgactcatactc                                                  >  1:801612/1‑141 (MQ=255)
                                                                                             tCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACCCAGAGAGTGATTCAGACTCAGATAGCGACTCAGAGTCAGCCCGCGCCCCAGCTTCAGACAGCgacccagcctca                                                 >  1:1096405/1‑141 (MQ=18)
                                                                                              cGGATAGCGATTCAGATTCAGACATCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCATTCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGactcagactcag                                                <  1:844782/141‑1 (MQ=255)
                                                                                               ggATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGActcagactcaga                                               >  2:3963221/1‑141 (MQ=255)
                                                                                                  tAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAg                                            <  1:295614/141‑1 (MQ=255)
                                                                                                  tAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAg                                            <  1:4009300/141‑1 (MQ=255)
                                                                                                   ggCGATTAAGAAGCAGACGGAGAATTAGATGCAGATAGCGATTTAGATTCTGATAGAGGATCAGATAGCGATGCAGAATCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGt                                           <  2:339541/140‑1 (MQ=17)
                                                                                                   aGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAga                                           >  1:3705164/1‑140 (MQ=255)
                                                                                                       attcatattcagATTTTGATTCAGATTTAGATGGTGATTTAGATTCTGATAGTGAATCAGATATTGATTCAGAATCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGAtt                                       <  2:2228211/141‑1 (MQ=17)
                                                                                                            gaagcagaCAGCGATTCAGAATCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGAc                                  <  1:4052954/137‑1 (MQ=255)
                                                                                                               tcagaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGAGACCCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCa                               >  1:1691419/1‑141 (MQ=255)
                                                                                                               ggagaCAGCGGTGTAGAGGCAGATAGAGAGTTAGAATCAGATAGAGAATCAGATAGTGATGCAGAATCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCa                               <  2:2379925/139‑1 (MQ=17)
                                                                                                                cagaCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAGAGCCACACAGACTCAGAGAGTGACACAGACTCAg                              >  2:3231310/1‑141 (MQ=18)
                                                                                                                 agaCAGAGGTTCATATTCACATAACGATTCAGAGTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGa                             <  2:1581/141‑1 (MQ=18)
                                                                                                                 agaCAGAGAAACAGATGACGATAGTGATTCAGAATTAGACAATGCATCATACAGAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGa                             <  2:2535368/141‑1 (MQ=14)
                                                                                                                   aCAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAATGAACCAGACTCAggtt                           >  2:4235624/1‑138 (MQ=18)
                                                                                                                      acGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCg                        <  1:3186977/140‑1 (MQ=255)
                                                                                                                          ttcagattcagaTAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTc                    >  1:2341178/1‑141 (MQ=255)
                                                                                                                          ttcagattcagaTAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTc                    >  1:679236/1‑141 (MQ=255)
                                                                                                                            cagattcagatAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAg                  <  1:492154/141‑1 (MQ=255)
                                                                                                                                   agatAGCGATTCAGAATCAGACAGTGAATAAGAAAGGGAAGAAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcaga           <  2:2027014/141‑1 (MQ=17)
                                                                                                                                    gatATCGATTCAGCTTCAGACAGAGACTCAGATAGTGATTCAGAGTCAGATAGCCACTCTCATTTATACAGCGTCTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagac          <  1:4373180/141‑1 (MQ=17)
                                                                                                                                    gatAGCGATTCAGACTCAGATAGAGACTCAGATAGTGACTCAGACTCAGATACCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGGCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagac          <  2:2815096/141‑1 (MQ=17)
                                                                                                                                     atAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagaca         >  1:2420655/1‑141 (MQ=255)
                                                                                                                                        gCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCACATTGAGACTCAGACTCAGAGAGCGACTCAGACtcagccaaga      >  2:2204653/1‑138 (MQ=14)
                                                                                                                                         cGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcga     <  1:2126341/141‑1 (MQ=255)
                                                                                                                                          gattcagatcaaGATAGAGATTCAGATAGTGATTCAGATTCAGATAGAGACTCAGATTCAGATAGTGACTCAGATTCAGACTGTGACTCAGACTCAGATAGTGATTCAGACTCATATAGCGACTCAGATtcagacagcgac    <  2:2736588/141‑1 (MQ=17)
                                                                                                                                           attcagattcagACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTGAGCTTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATTGTGGTTCAGAGTCGTATAGCGACTAAGAAacagacagcgact   >  2:3911908/1‑141 (MQ=17)
                                                                                                                                            ttcagattcagaCAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATtcagacagcgactg  >  1:727628/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GGATACTACGAAGAAGAAACATCAGATAGCGACTCAGATTCGGACAGCGACTCAGATTCAGACAGAGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTC  >  USA300TCH1516_ALE/618247‑618527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: