Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,636 G→A 6.8% S1246S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6360GA 6.8% 1030.1 / 7.2 433S1246S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (211/162);  new base A (30/14);  total (246/187)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 437 total aligned reads displayed.

AGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGA  >  USA300TCH1516_ALE/618498‑618776
                                                                                                                                           |                                                                                                                                            
 gATAGCGACTCACATCAAAGAAGCTACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                                           <  1:590586/141‑1 (MQ=17)
       gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                                     >  1:2968886/1‑141 (MQ=255)
        aCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATa                                                                                                                                    <  1:1486222/141‑1 (MQ=255)
         cgcAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                                   <  1:4113054/139‑1 (MQ=255)
         cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                                   >  1:3968393/1‑141 (MQ=255)
         cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAATCAGACCAGGACAGCGATTGAGACTCGGAAAGCGACTCAGATACAAATAGCGATACGGGCTAAGAtta                                                                                                                                   >  2:3335864/1‑139 (MQ=14)
           cAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                                 <  1:3799148/141‑1 (MQ=255)
            aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAATCAGACTTCGAGAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGa                                                                                                                                >  2:525232/1‑141 (MQ=14)
             gATTCAGCGCGCGAAGCAGACACAGACAGCGACACAGAATCAGATAGAGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                               <  2:2401498/141‑1 (MQ=14)
             gATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGAGAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                               <  1:11835/141‑1 (MQ=255)
              aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCgccc                                                                                                                              >  1:1412986/1‑138 (MQ=255)
               ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGTTTCAGAGAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                             <  1:3453699/141‑1 (MQ=255)
                tcagacagcgactcagactcagacagcgactcagacTCAGATAGTGACTCAGATTCAGATAGCGACTGAGATTCGCACAGCGAGTCAGACTCAGAAAGCGACTCAGATTCAGATAGCGAGTCGGAACCAGATAGCGACtca                                                                                                                            >  2:867571/1‑141 (MQ=14)
                tcagacagcgactcagactcagacagcgactcagacTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtca                                                                                                                            <  1:3020759/141‑1 (MQ=255)
                 tggacaacgagtcagcctcacacagcgactcagactcaGATAGTGACTGAGATTCAGATAGCGACTCAGATTCGGACAGAGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcag                                                                                                                           <  2:4246873/139‑1 (MQ=17)
                 cagacagcgactcagactcagacagcgactcagactCAGATAGTGACTCAGATTCAGAAAGCGCCTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGCCTCCGAATCAGATAGCGACgcag                                                                                                                           >  2:11223/1‑141 (MQ=14)
                  agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATCACGACGCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                                                                                                          >  1:4159392/1‑141 (MQ=255)
                    caagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagagt                                                                                                                        >  1:1931176/3‑139 (MQ=255)
                        cgacttcgacgcagatagcgacttagcctctgaCAGTGACTCAGCGTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                                                                    <  2:3072499/141‑1 (MQ=14)
                        cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                                                                    >  1:3876663/1‑141 (MQ=255)
                           ctccgactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                 <  1:4014839/141‑1 (MQ=255)
                           ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                 >  1:2008335/1‑141 (MQ=255)
                              aaactcagactgcgattcagattcagaTAGTGATTCAGATTCAGATAGCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                                              <  1:1145315/139‑1 (MQ=14)
                               gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                                                                             >  1:2930472/1‑141 (MQ=255)
                               gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                                                                             <  1:2371801/141‑1 (MQ=255)
                               gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGATAGTGATTCGGAGTCAGATAGCGACTCAGAGTCAGAGCGTGag                                                                                                             >  2:2233185/1‑140 (MQ=17)
                               gactcagacaccgactcagactcagaTAGTGACTCAGATTCAGATAGTGACTCAGATTCCGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                                                                             <  2:640794/141‑1 (MQ=255)
                                actcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                                            >  1:1556867/1‑141 (MQ=255)
                                    agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                                        >  1:3080225/1‑141 (MQ=255)
                                     gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGCTTCAGATAGTGATTCAGAc                                                                                                       >  1:2465006/1‑141 (MQ=255)
                                       cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                     <  1:4218066/141‑1 (MQ=255)
                                       cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                     <  1:3670590/141‑1 (MQ=255)
                                        agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                                    >  1:480438/1‑141 (MQ=255)
                                        agcgactcagactcagaTAGAGACTCAGACTCAGATAGCGACTCACACTCAGACAGCGATTCAGACTCAGATAGCGACACAGAAGCAGATAGCGCCTCGGACTCAGATAGCGACTCAGATGCAGACAGGAATTAAGACGCa                                                                                                    >  2:4095424/1‑141 (MQ=11)
                                         gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAg                                                                                                   >  1:678864/1‑141 (MQ=255)
                                          cgactcagactcataTAGTTAGTCAGATTCAGATAGAATCTCAGATTCAGACAGGGAATCAAACTAAAATAGTGACTCAGATTCAGATAGCGATTCGGACCCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                  <  2:1583174/141‑1 (MQ=17)
                                          cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                  <  1:1548507/141‑1 (MQ=255)
                                              tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGc                                                                                              >  1:724088/1‑141 (MQ=255)
                                              tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACTCAGATTCAAATAGTGATTCACACTAAGATaaa                                                                                              >  2:3284172/1‑139 (MQ=14)
                                               cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGGTAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg                                                                                             >  1:2598506/1‑141 (MQ=255)
                                                  actcagaTAGTGAGTGAGATTCAGACAGGGACTCAGAGTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACt                                                                                          <  2:342546/141‑1 (MQ=14)
                                                   tgcagaTACTGACGCAGATTCAGACGGCGAGTGAGATTCGGAGAGCGATTCAGTGTTGTATGTGTACTCGGACTCAGAGAGCGACACGGACTCAGATAGCGGCTCAGAGTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                                         <  2:1373564/139‑1 (MQ=9)
                                                   ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                                         >  1:3480570/1‑141 (MQ=255)
                                                   ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                                         <  1:888333/141‑1 (MQ=255)
                                                        aTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACGCAGacc                                                                                    >  2:396501/1‑139 (MQ=11)
                                                         tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTc                                                                                   <  1:4249858/141‑1 (MQ=255)
                                                         tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATACAGAAAGTGATTCAGACAGAGAAAGCGACTCAAAtac                                                                                   >  2:2806689/1‑139 (MQ=14)
                                                         tAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGAGATTCAGACTGAGAAAGCGACTCAGATTCAGAGAGCGACTCGGACTCAGCTAGCGGATTAGAATGAGATAGCGATTCACACCCCGCCAGCGACTCAGaagc                                                                                   >  2:4175406/1‑138 (MQ=2)
                                                          aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATACAGATAGTGATTCAGACTCAGATAGCgactcagactca                                                                                  >  2:855297/1‑141 (MQ=255)
                                                               cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGAGATTCAGACTCAGGTAGCGACTCAGATTCAGaaaa                                                                             >  2:790929/1‑138 (MQ=14)
                                                                  agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                          <  2:320847/141‑1 (MQ=255)
                                                                  agattcagatAGCCATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                          <  2:1227644/141‑1 (MQ=255)
                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAcc                                                                        >  1:817343/1‑140 (MQ=255)
                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                        >  1:3538555/1‑141 (MQ=255)
                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATATCGACTAGGAGTCAGATAGCGACTCAGGTTCAGATCGCGAGTGAGAATCAGATCTCGACTCAAGTCCAGAcctcgaag                                                                        >  2:2119843/1‑134 (MQ=2)
                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCACACTCAGATAGCGCCACAGATTCACACCGCGAcc                                                                        >  2:2547596/1‑140 (MQ=17)
                                                                    attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTAGGACTCAGATAGCGACTCAGATACAAATAGTGATTCACACTCAGATAGCGCCCCAGAATCACCCAGAGAcg                                                                        >  2:367154/1‑140 (MQ=14)
                                                                       cagatAGCGACTCAGATTCGGACAGCGATTCAGACTAAGGACCGGCGCCAGAGTCAGGTTGTGGGAAACACCCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                     >  1:4393139/1‑141 (MQ=9)
                                                                        agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                                    >  2:1914502/1‑141 (MQ=255)
                                                                           tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTc                                                                 >  1:4073174/1‑141 (MQ=255)
                                                                            aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                >  1:848665/1‑141 (MQ=255)
                                                                            aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                <  1:524733/141‑1 (MQ=255)
                                                                             gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCgg                                                               >  1:2885559/1‑141 (MQ=255)
                                                                             gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCgg                                                               >  1:1821317/1‑141 (MQ=255)
                                                                              cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGa                                                              >  1:3734397/1‑141 (MQ=255)
                                                                               gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAt                                                             <  1:2547323/141‑1 (MQ=255)
                                                                               gACTCAGACGCAGACAGCGATCTAGATTCTGATAGCGACTCAGATTCAGATAGCGATTCTGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGAt                                                             <  2:1691419/141‑1 (MQ=14)
                                                                                aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATa                                                            >  1:339208/1‑141 (MQ=255)
                                                                                 cTCAGATTCGGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                                           <  1:1595948/141‑1 (MQ=255)
                                                                                           gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                                                 <  1:1614513/141‑1 (MQ=255)
                                                                                           gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCAATTCGGACTCAGATAGCGACTCAGATTGAGACAGTGATTCAGAAACAGTGTGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                                                 >  1:2783597/1‑141 (MQ=17)
                                                                                              tccGATTCAGTATCCGATAGTGATTCAGTTTCCGACAGTCATTCTGCCTCAGATACCGACTCGGACTCAGATCGTGTTGCTGACCCAGATAGTGATTCAGATTCAGACAGCGACTCTGATTCGGATAGCGACTCAGATTCa                                              <  2:3151175/139‑1 (MQ=9)
                                                                                              aGCGATTCAGGTGCAGAAAGCGACTCAGATTCAGATAGAGACTCAAACTGAGATGGTTACTCAGAGTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                              <  2:727628/141‑1 (MQ=17)
                                                                                              aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                              <  1:1372644/141‑1 (MQ=255)
                                                                                                   ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAATCAGACACAGCTAGCGACCCAGATACAGataa                                         >  2:4375258/1‑138 (MQ=14)
                                                                                                      aGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa                                      <  1:2204653/141‑1 (MQ=255)
                                                                                                         cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                                   <  1:909961/141‑1 (MQ=255)
                                                                                                            agagagCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                                <  1:2433068/141‑1 (MQ=255)
                                                                                                            aGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                                >  1:1301988/1‑141 (MQ=255)
                                                                                                             gATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGAc                               >  1:223599/1‑141 (MQ=255)
                                                                                                             gATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCAACTCACACTCACATAGCGACCCAGATCCAGAAAGCCACGCacac                               >  2:2748680/1‑141 (MQ=14)
                                                                                                              aTAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATACACCTAGCGACCCAGATTCAGATAGCGACACACACt                              >  2:3556035/1‑141 (MQ=11)
                                                                                                               tAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc                             >  1:2750644/1‑141 (MQ=255)
                                                                                                                aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCg                            >  1:3300323/1‑141 (MQ=255)
                                                                                                                aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCg                            >  1:4288904/1‑141 (MQ=255)
                                                                                                                   gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAt                         >  1:2839210/1‑141 (MQ=255)
                                                                                                                       tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                      >  1:1039245/1‑141 (MQ=255)
                                                                                                                       tcagattcagatAGCGATTCGGACTCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                      <  1:2687674/141‑1 (MQ=255)
                                                                                                                          gaatcagagAGCGACTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                   <  1:981043/138‑1 (MQ=255)
                                                                                                                            ctcagatAGCTATCCAGACTCAAAGAGCGACCCACAATCGGAAAGTGAGCCAGACTCGGATAGCGACTCAGACACAGACAGCGACCCAGATTCGGATAGCGACTCAGATTCAGACAGAGCCTCAGACTCGGATAGTGATTc                 <  2:1612328/140‑1 (MQ=9)
                                                                                                                             tcagatAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCa                >  1:3177225/1‑141 (MQ=255)
                                                                                                                                gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAACGACTCAGACTCGGATAGACATCCAGAc             >  2:2975116/1‑141 (MQ=17)
                                                                                                                                  tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGCCACCGGCAACGGGTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc           >  1:3211500/1‑141 (MQ=17)
                                                                                                                                  tAGCGATTCGGACGCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc           <  1:3911908/141‑1 (MQ=255)
                                                                                                                                   ggCGAGTCGGACTCAGAGAGCGACTCAGATGCCCATCGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCTGATTCTGAGAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCa          <  2:2930472/140‑1 (MQ=17)
                                                                                                                                   aGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCa          <  1:3493910/141‑1 (MQ=255)
                                                                                                                                   aGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAAAGTCACGGACACTCAGACAGCGACTCAGACTCGGATAGACATTCAGACTCa          >  2:1782748/1‑141 (MQ=17)
                                                                                                                                        ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAg     >  2:3829888/1‑141 (MQ=255)
                                                                                                                                        ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCTGATAGTGATTCAGAACCAGATaa     >  2:385971/1‑140 (MQ=14)
                                                                                                                                           ggACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGCga  >  2:1492333/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGA  >  USA300TCH1516_ALE/618498‑618776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: