Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,666 T→C 5.3% S1256S (AGT→AGC)  sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6660TC 5.3% 1023.7 / 3.6 433S1256S (AGT→AGC) sdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (230/157);  new base C (20/11);  total (257/176)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 440 total aligned reads displayed.

TCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGA  >  USA300TCH1516_ALE/618526‑618806
                                                                                                                                            |                                                                                                                                            
 gagactcagagagcgacgcagactcagaGACGGACACAGACTAGGAGAGCGACTCAGATTCGGATAGTGATTGAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                                                                                                                             <  2:843640/140‑1 (MQ=14)
   gactcagacagcgactcagactcagaTAGTGCATCACACCCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAATCAGCTTCAGATAGCTACTCGTGCTCAAATAGCGTCTAAAATGCAAATAATGAt                                                                                                                                           >  2:2021282/1‑141 (MQ=9)
   gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                                                                                                           >  1:2930472/1‑141 (MQ=255)
    actcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                                                                          >  1:1556275/1‑141 (MQ=255)
      tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGCCAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATGCa                                                                                                                                        >  2:2175793/1‑141 (MQ=255)
        agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                                                                      >  1:2589131/1‑141 (MQ=255)
        agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAGACAGTTAGTGGTTCAgg                                                                                                                                      >  1:1658683/1‑140 (MQ=255)
         gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCGGACAGCGATTCCGACTCAGACAGCGACTCAGATTCAGATCGCGATACAGACTCCCATCGCGACTCAGACTCACATAGCGACTCAGAc                                                                                                                                     >  2:1636390/1‑141 (MQ=14)
           cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                                                   >  1:789298/1‑141 (MQ=255)
           cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                                                   <  1:4218066/141‑1 (MQ=255)
           cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                                                   <  1:1541035/141‑1 (MQ=255)
           cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAGTCGGACTCAGATAGCGACTCAGATGCGGATAGTGAATAGGACTc                                                                                                                                   >  1:1584266/1‑141 (MQ=17)
            agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATGGTGATTCAGACTCa                                                                                                                                  >  1:3983684/1‑141 (MQ=255)
            agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                                                                  >  1:2403047/1‑141 (MQ=255)
            agcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                                                                  >  1:480438/1‑141 (MQ=255)
             gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGAACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATACTGATTCAGACTCAg                                                                                                                                 >  1:2456417/1‑141 (MQ=255)
              cgactcagactcataTAGTTAGTCAGATTCAGATAGAATCTCAGATTCAGACAGGGAATCAAACTAAAATAGTGACTCAGATTCAGATAGCGATTCGGACCCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                                                <  2:1583174/141‑1 (MQ=17)
              cgactcagactcagaTAGTGACTCAGATTCAGCTCGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAATCAGACGCAGATAGCGAATCAGATTCACATAGAGAAAGAGACTCaaa                                                                                                                                >  2:4359485/1‑139 (MQ=14)
              cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                                                >  1:2988786/1‑141 (MQ=255)
               gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAAAGCGACTCAGACGAAGATAGCGCGTCAGACGCAGAt                                                                                                                               >  2:702402/1‑141 (MQ=14)
                   cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACACAGACTCAGATAGCGATTCAGACTCAGATacag                                                                                                                           >  2:1744004/1‑138 (MQ=14)
                              aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTGAGATCGTGATTCAGCCTCAGATAGCGCGTCAGATTcc                                                                                                                >  1:1608749/1‑140 (MQ=14)
                              aGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                                                >  1:3300813/1‑141 (MQ=255)
                                   cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGATAGTGAATCAGACTCAGATCGCTACTCAGAACCAGACAg                                                                                                           >  2:3540457/1‑141 (MQ=14)
                                    tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGAGATTCAGAGGGTGATAGCGACTCAGATTCAAACAGc                                                                                                          >  2:2688974/1‑141 (MQ=17)
                                        attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAcc                                                                                                      >  1:817343/1‑140 (MQ=255)
                                        attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                                                      >  1:2373564/1‑141 (MQ=255)
                                        attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                                                      >  1:3538555/1‑141 (MQ=255)
                                        attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGAAAGCGAcc                                                                                                      >  2:1501139/1‑140 (MQ=255)
                                        attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTAGGACTCAGATAGCGACTCAGATACAAATAGTGATTCACACTCAGATAGCGCCCCAGAATCACCCAGAGAcg                                                                                                      >  2:367154/1‑140 (MQ=14)
                                             gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAt                                                                                                 >  1:3729527/1‑141 (MQ=255)
                                                aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCg                                                                                              >  1:848665/1‑141 (MQ=255)
                                                aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCACACAGCGACTCAGATTCg                                                                                              >  1:1135654/1‑141 (MQ=255)
                                                aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTTCGATTCAGACAACGAAACAGATTCg                                                                                              >  2:3734169/1‑141 (MQ=14)
                                                 gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCgg                                                                                             >  1:1821317/1‑141 (MQ=255)
                                                    aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATa                                                                                          >  1:339208/1‑141 (MQ=255)
                                                      tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                                                        >  1:4084724/1‑141 (MQ=255)
                                                          aTTCGGACAGCGATTCCGATTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACt                                                                                    <  2:3979152/141‑1 (MQ=255)
                                                          aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAGTCAGACAGCGACTCAGAATCAGATAGCGatc                                                                                    >  2:1176180/1‑139 (MQ=255)
                                                               gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCAATTCGGACTCAGATAGCGACTCAGATTGAGACAGTGATTCAGAAACAGTGTGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                                                                               >  1:2783597/1‑141 (MQ=17)
                                                                  tccGATTCAGTATCCGATAGTGATTCAGTTTCCGACAGTCATTCTGCCTCAGATACCGACTCGGACTCAGATCGTGTTGCTGACCCAGATAGTGATTCAGATTCAGACAGCGACTCTGATTCGGATAGCGACTCAGATTCa                                                                            <  2:3151175/139‑1 (MQ=9)
                                                                  aGCGCTACAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                                                            <  1:4131986/141‑1 (MQ=255)
                                                                  aGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                                                            <  1:1372644/141‑1 (MQ=255)
                                                                    cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGa                                                                          >  1:2536382/1‑141 (MQ=255)
                                                                     gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGAc                                                                         <  1:4258173/141‑1 (MQ=255)
                                                                       ttCCGACTCAGATAGCGCCTCAGATCCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAg                                                                       <  1:894970/141‑1 (MQ=255)
                                                                       ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAg                                                                       >  1:2208707/1‑141 (MQ=255)
                                                                        tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc                                                                      <  1:3643292/141‑1 (MQ=255)
                                                                        tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc                                                                      >  1:55917/1‑141 (MQ=255)
                                                                            aCTCAGATGGAGACGCAGATTCAGATAGAGATTCCGACTCAGATAGAGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACt                                                                  <  2:3047775/141‑1 (MQ=17)
                                                                             cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                                                                 <  1:909961/141‑1 (MQ=255)
                                                                                aGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                              >  1:3080738/1‑141 (MQ=255)
                                                                                    aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCg                                                          >  1:3243476/1‑141 (MQ=255)
                                                                                    aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCg                                                          >  1:4288904/1‑141 (MQ=255)
                                                                                      ccaCTCAGATTCAGATAGCCATTCCGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                                                        <  1:2138262/139‑1 (MQ=255)
                                                                                          tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                                                    >  1:1039245/1‑141 (MQ=255)
                                                                                             gattcagatAGCGAGTCGGACTACGACAGCGACTCAGAGTCATATAGGGATACAGGCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                                                 <  2:865869/141‑1 (MQ=17)
                                                                                             gactcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGAt                                                 <  1:1423271/138‑1 (MQ=255)
                                                                                               cacagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                               <  2:2420655/139‑1 (MQ=255)
                                                                                                     taggcaTTCGGACTTAGATAGAGATTGAGATTCAGCTAGTGACTCATACTCAGATAGCGATTCAGATTCAGACAGAGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                         <  2:2914695/136‑1 (MQ=17)
                                                                                                     tAGGGATTCAGACTCAGATATGGACTTAGATTCAGATAGTGATTCAGGCTCAGAGAGTGACTTAGATTTAGATTTGGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                         <  1:4380366/141‑1 (MQ=17)
                                                                                                     tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTc                                         >  2:1884858/1‑141 (MQ=255)
                                                                                                     tAGCGATTCGGACGCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                         <  1:3911908/141‑1 (MQ=255)
                                                                                                      aGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCa                                        <  1:3493910/141‑1 (MQ=255)
                                                                                                         gATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGAt                                     <  1:3831724/141‑1 (MQ=255)
                                                                                                           ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                                   >  2:279840/1‑141 (MQ=255)
                                                                                                           ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCTGATAGTGATTCAGAACCAGATaa                                   >  2:385971/1‑140 (MQ=14)
                                                                                                            tccgACTCAGATGTCGACTCAGATTCAGATAGTGATTCAGACTCTGTTAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGc                                  <  2:496101/138‑1 (MQ=18)
                                                                                                            tCGGACTAAGATAGCGACTAAGATTCAGATAGTGATACAGACTCAGATAGCGACTCAGAAACAGAAAGCGACTCAGATTCAGATAGCGACACAGAATCAGACAGAGACTCAGACCCAGACAGAGACACAGACCGAGAAAGc                                  >  2:4210436/1‑141 (MQ=2)
                                                                                                             cGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCg                                 >  1:3459230/1‑141 (MQ=255)
                                                                                                                aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgact                              >  1:2637273/1‑141 (MQ=255)
                                                                                                                aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAGTGAGATAgagagt                              >  2:1409388/1‑139 (MQ=17)
                                                                                                                 cTCAGATAGTGCTTCTGATTCAGATAGCGAGTCAGACTCAGATAGCGCCTCAGACTCAGATAGCGACTCAGACTCCGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                             <  2:2530490/141‑1 (MQ=14)
                                                                                                                 cTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                             >  1:3140867/1‑141 (MQ=255)
                                                                                                                 cTCAGATAGCGACTCAGAGTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                             <  1:192563/141‑1 (MQ=255)
                                                                                                                  tCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACACCCACTCACACTCGGATAGTGACTCCGACTCAACTAACgactcc                            >  2:766477/1‑140 (MQ=17)
                                                                                                                    aGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACACAGACTCGGATAGTGATACAGACACAAATAGAaactcaga                          >  2:3291985/1‑141 (MQ=17)
                                                                                                                       tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                       >  2:3213656/1‑141 (MQ=255)
                                                                                                                       tAGCGACTCAGATGCAGCTAGTGACTCAGACTCATATAGCGACTCAGATTCACACACCGCCTCCGATTCTGATAGCGACTCCGATTCTTACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                       <  2:4073174/141‑1 (MQ=18)
                                                                                                                        aGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactca                      >  1:4400164/1‑141 (MQ=255)
                                                                                                                        aGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTAAGACTCAGATAGCgaatcagaccca                      >  2:1348102/1‑141 (MQ=255)
                                                                                                                         gCGACTCAGACTCAGATAGGTGCTCAGATTCAGATAGCGACTCAGAGTCCGACAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGactcagactcag                     <  2:597357/141‑1 (MQ=14)
                                                                                                                          cGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGCCTCACATACcgactccgactccca                    >  2:1000660/1‑138 (MQ=17)
                                                                                                                             cTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTGCGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGTGACTCAGACTCAGATAGCGACTCAGAATTAGATAg                 >  2:1806361/1‑141 (MQ=14)
                                                                                                                             cTCAGATTCACATACTGACTCAGCCTCAGACAGCGACTCTGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAg                 <  2:2968886/141‑1 (MQ=18)
                                                                                                                              tcagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGc                <  1:28416/141‑1 (MQ=255)
                                                                                                                              tcagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGACAGCGACGCAGACTCGGATAGCGATTCAGACTCACCTAGCGAagccgactcagacaac                >  2:3401935/1‑137 (MQ=17)
                                                                                                                                 gattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCCGACTCAGATAGGGAGCCAGACTCAGATAGCgat             >  1:3796498/1‑141 (MQ=255)
                                                                                                                                  attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGAGTCAGACTCAGATAGCgatt            >  1:1190936/1‑141 (MQ=255)
                                                                                                                                    tcagatAGTGATTCAGCCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattca          <  1:2876138/141‑1 (MQ=255)
                                                                                                                                    tcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattca          <  1:2672713/141‑1 (MQ=255)
                                                                                                                                     cagatAGTGATTCCGGCTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcag         <  1:2755364/141‑1 (MQ=255)
                                                                                                                                       gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGActcagactcaga                                                                          >  2:2311476/1‑74 (MQ=25)
                                                                                                                                       gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGa                                                                          <  1:2311476/74‑1 (MQ=255)
                                                                                                                                       gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagat       >  1:3973582/1‑141 (MQ=255)
                                                                                                                                       gatAGCGATTCTGACTCAGATAGCGACTCAGTTTAAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGATAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagat       <  2:1095173/141‑1 (MQ=17)
                                                                                                                                         tactGACTCAGACGCAGAGAGCGACTCAGATTCAGACAGCGTCTCAGATGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattc     <  2:4132331/138‑1 (MQ=14)
                                                                                                                                         tAGGGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACCAAGCTCGTGCATCAGACTCAGATAGCgattcagattc     <  1:3052057/141‑1 (MQ=18)
                                                                                                                                          aGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattca    <  1:702402/141‑1 (MQ=255)
                                                                                                                                            tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAttcagattcaga  >  1:1292070/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGA  >  USA300TCH1516_ALE/618526‑618806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑