Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,714 G→A 6.4% S1272S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,7140GA 6.4% 1093.9 / 9.1 437S1272S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (188/196);  new base A (22/20);  total (214/223)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 453 total aligned reads displayed.

AGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGA  >  USA300TCH1516_ALE/618574‑618854
                                                                                                                                            |                                                                                                                                              
aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTGAGATTCg                                                                                                                                                >  2:3709533/1‑141 (MQ=255)
aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCACACAGCGACTCAGATTCg                                                                                                                                                >  1:1135654/1‑141 (MQ=255)
 gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCgg                                                                                                                                               >  1:1821317/1‑141 (MQ=255)
     ctgaGAATCGGACAGCGATTCATGCTCAGATTTCTATTTATTTTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACGCAGATAGAGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAg                                                                                                                                           <  1:68323/138‑1 (MQ=17)
      tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                                                                                                          >  1:3073176/1‑141 (MQ=255)
       cAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCg                                                                                                                                         <  1:2882134/141‑1 (MQ=255)
            tCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCa                                                                                                                                    <  1:525232/141‑1 (MQ=255)
               gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAt                                                                                                                                 <  1:1614513/141‑1 (MQ=255)
                        tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATGGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGc                                                                                                                        >  1:2570408/1‑141 (MQ=255)
                          aGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa                                                                                                                      <  1:2204653/141‑1 (MQ=255)
                            aCTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACt                                                                                                                    <  1:1571969/141‑1 (MQ=255)
                              tCAGATAGCGACTCAGATTCAGATAGCGATTCAGATTCTGGTGGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCa                                                                                                                  <  1:1206228/141‑1 (MQ=17)
                               cAGATTGCGACGCTGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                                                                 <  2:3981069/141‑1 (MQ=14)
                               cAGATAGGGATTCAGATTCAGATAGCGATTGCGATTCAGATAGCTACTCCGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                                                                 <  2:2206628/141‑1 (MQ=17)
                               cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                                                                 <  1:4403419/141‑1 (MQ=255)
                               cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                                                                 <  1:3839319/141‑1 (MQ=255)
                                   tAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc                                                                                                             <  1:3723873/141‑1 (MQ=255)
                                    aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATGCGGCTACCGACTCAGAGTCACACAGCGAACCAGACTcc                                                                                                            >  2:1408577/1‑140 (MQ=17)
                                    aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCTGACGCCGACCCAGCATCAGACACCGAcacaccctcc                                                                                                            >  2:93265/1‑135 (MQ=11)
                                      agagtctgatgcagatAGCGACTCGGACTCAGATACCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGa                                                                                                          <  2:3093767/134‑1 (MQ=17)
                                          tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                                                                                                      >  1:1039245/1‑141 (MQ=255)
                                            agattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGACTCAGACTCAGATAGCAACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCTGATTCAGACAGCGACTCAGACTCGGATAGTGa                                                                                                   <  2:3903976/141‑1 (MQ=255)
                                               ttcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGCTAGCGTCTCAGAGTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                <  1:856971/141‑1 (MQ=255)
                                               cacagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTc                                                                                                <  2:2420655/139‑1 (MQ=255)
                                                 cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTCAGTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAg                                                                                             <  1:2380601/141‑1 (MQ=255)
                                                  agatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACACAGACTCAGACAGCGCCACAGACTCGGATAGTGATGCaca                                                                                             >  2:2873302/1‑139 (MQ=17)
                                                   gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAACGACTCAGACTCGGATAGACATCCAGAc                                                                                           >  2:2975116/1‑141 (MQ=17)
                                                     tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGCCACCGGCAACGGGTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTc                                                                                         >  1:3211500/1‑141 (MQ=17)
                                                     tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGAcgc                                                                                         >  1:2434217/1‑139 (MQ=255)
                                                      aGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAAAGTCACGGACACTCAGACAGCGACTCAGACTCGGATAGACATTCAGACTCa                                                                                        >  2:1782748/1‑141 (MQ=17)
                                                        cGAGTCGGACTCAGATAGCGACTCAGATTCAGACAGTGATTCAGACGCAGATAGCGACTCAGATTCAGACCGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGCCTCGGATAGTGATTCAGACTCAGa                                                                                      <  1:908238/141‑1 (MQ=18)
                                                         gagtCGGACTCTGATTGTAAGACAGATTCAGATAGGGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGAt                                                                                     <  2:3277777/138‑1 (MQ=17)
                                                         gATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGAt                                                                                     <  1:81573/141‑1 (MQ=255)
                                                           ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                                                                                   >  1:1515805/1‑141 (MQ=255)
                                                           ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg                                                                                   >  1:2996464/1‑141 (MQ=255)
                                                           ttCGGACTCAGATAGCGACTCAGAATCAGATAGTGATTCAGACTCAGATAGCGACTCAGATCCAGAAAGCGACTCATACTCAGATAGAGAAACAGAATCAGAAAGCGCCTCACACACAAATAGTGACTCAGACTAACATAg                                                                                   >  2:3318010/1‑141 (MQ=9)
                                                            ttggccTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGc                                                                                  <  1:2688974/136‑1 (MQ=255)
                                                             cGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCg                                                                                 >  1:3459230/1‑141 (MQ=255)
                                                             cGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACACAGACCCGCATAGTCATTCACACTCAGATAGCg                                                                                 >  2:2942017/1‑141 (MQ=17)
                                                                aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgact                                                                              >  1:2670079/1‑141 (MQ=255)
                                                                aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATACAGACACAGATAGCgaca                                                                              >  2:1152722/1‑140 (MQ=255)
                                                                aCTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGACAGCGACACAGACTCGAATTGACATTCATACTCAGATAGCcact                                                                              >  2:1083095/1‑141 (MQ=17)
                                                                 cacAGTTAGCGACTCAGATTCAGATAGCCAGTCATATACAGATAGCGACTCAGAGACAGACAGCCACTCAGAGTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                                                                             <  2:506144/139‑1 (MQ=14)
                                                                 cTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                                                                             >  1:3140867/1‑141 (MQ=255)
                                                                 cTCAGATAGCGACTCAGAGTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactc                                                                             <  1:192563/141‑1 (MQ=255)
                                                                  tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactca                                                                            >  2:4080308/1‑141 (MQ=255)
                                                                  tCAGACAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACACCCACTCACACTCGGATAGTGACTCCGACTCAACTAACgactcc                                                                            >  2:766477/1‑140 (MQ=17)
                                                                   cAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcag                                                                           >  1:4315733/1‑141 (MQ=255)
                                                                   cAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGATTCAGACAGCAACCCAGACTCGGCTAGTGACTCACACCCAGATAGCgactcag                                                                           >  2:268557/1‑141 (MQ=17)
                                                                       tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                                                                       >  2:3213656/1‑141 (MQ=255)
                                                                       tAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                                                                       <  1:3811707/141‑1 (MQ=255)
                                                                       gaGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactc                                                                       <  1:4282150/140‑1 (MQ=255)
                                                                        aGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCGGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactca                                                                      >  1:3038560/1‑141 (MQ=255)
                                                                        aGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCgactcagactca                                                                      >  1:4104760/1‑141 (MQ=255)
                                                                              tcagattcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGc                                                                <  1:28416/141‑1 (MQ=255)
                                                                                  attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGAGTCAGACTCAGATAGCgatt                                                            >  1:1190936/1‑141 (MQ=255)
                                                                                  attcagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgatt                                                            >  1:4017865/1‑141 (MQ=255)
                                                                                     cagatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgcctcag                                                         >  1:2046403/1‑141 (MQ=255)
                                                                                       gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTTAGATAGCGACTCAGATTCAGACAGCGACCCAGACTCGGATAGTGATTCAGACCCAGATAGAGACACAAACTCAGATAGCgaaccacat                                                       >  2:1858632/1‑134 (MQ=17)
                                                                                       gatAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGa                                                                                                                            <  1:2311476/74‑1 (MQ=255)
                                                                                         tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattc                                                     <  1:139219/141‑1 (MQ=255)
                                                                                         tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGCTAGCgaatcagatac                                                     >  2:1564250/1‑139 (MQ=11)
                                                                                          aGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCgattcagattca                                                    >  1:1658303/1‑141 (MQ=255)
                                                                                            tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCACATAGCgattcagactcaaa                                                  >  2:634604/1‑139 (MQ=255)
                                                                                             gATTTAGATTGAGATAGTGATTAAGATCTTGACAGCGACTCAGATTCTGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATtcagattcagat                                                 <  2:350214/141‑1 (MQ=17)
                                                                                             gATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGGTGGCGCCTCAGATTCAGACAGCGACTCAGATTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATtcagattcagat                                                 <  1:4175406/141‑1 (MQ=255)
                                                                                               ttCATACTGATATAGCGACTCAGAATCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                               <  2:3769664/141‑1 (MQ=14)
                                                                                               ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                               <  1:1326444/141‑1 (MQ=255)
                                                                                                tgtgACTCTGATAGTGACTCAGATTCAGACAGCGAGTCAGATTCGGATAGCGACACAGATTCAGAAAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGc                                              <  2:3591738/138‑1 (MQ=17)
                                                                                                tCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGc                                              >  1:2605679/1‑141 (MQ=255)
                                                                                                 cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATGGCGACTCACACACAGCTACCGATTCACATACAGATacca                                             >  2:28324/1‑138 (MQ=18)
                                                                                                 cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATCGCGACTCAGACTCAGATCGAGACTCAGACACACCTAGCGGCTCAGATTCAGATcgcg                                             >  2:1300804/1‑141 (MQ=14)
                                                                                                  aGACTCAGATAGCGATGTAGAGTCATACAGCGACTCAGAGTCTGATTGCCACCCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCga                                            <  2:809572/141‑1 (MQ=11)
                                                                                                  aGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCga                                            >  1:1090501/1‑141 (MQ=255)
                                                                                                   gACTCACATAGCGACTCAGATTAGAACAACGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgac                                           <  1:3407414/141‑1 (MQ=18)
                                                                                                    aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTGAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGGTAGCGATTCAGATTCAGATAGCgact                                          >  1:1476450/1‑141 (MQ=255)
                                                                                                    aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCACACACAGATAGCGAATCAGGTACAGACAGCgcct                                          >  2:1500691/1‑141 (MQ=11)
                                                                                                      ccAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactca                                        <  1:4242965/140‑1 (MQ=255)
                                                                                                       gaGATGGCGTCTGAGAGTCAGATAGCGACTCAGATTCGGATAGCGTCTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcag                                       <  2:339208/140‑1 (MQ=17)
                                                                                                       cAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcag                                       >  2:1636718/1‑141 (MQ=255)
                                                                                                          aTAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGa                                                                                                                                        <  1:2767159/43‑1 (MQ=255)
                                                                                                           tAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCgactcagacac                                   >  2:4188391/1‑139 (MQ=255)
                                                                                                              ggcctcagactcagaCAGCGAATCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGActcagactcaga                                <  2:2335870/138‑1 (MQ=255)
                                                                                                                aCTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACa                              <  1:3496560/141‑1 (MQ=255)
                                                                                                                    aGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGa                          <  1:4383989/141‑1 (MQ=255)
                                                                                                                     gATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAt                         <  1:3969431/141‑1 (MQ=255)
                                                                                                                      aTTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGAtt                        >  1:3609367/1‑141 (MQ=255)
                                                                                                                        tCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCACACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtca                      >  1:2820319/1‑141 (MQ=255)
                                                                                                                         cAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcag                     >  1:955691/1‑141 (MQ=255)
                                                                                                                         cAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcag                     >  1:2746611/1‑141 (MQ=255)
                                                                                                                           gACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATtcagac                   >  1:2527678/1‑141 (MQ=255)
                                                                                                                              aGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGCTAGCGACTCAGACTCAGACAGCGATtcagactca                >  1:3736731/1‑141 (MQ=255)
                                                                                                                                   cTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAg           >  1:3370263/1‑141 (MQ=255)
                                                                                                                                    tcagattcagatAGAGACTCACACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGAGAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGc          <  2:4391769/141‑1 (MQ=11)
                                                                                                                                     cAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTc                                                                                                             >  1:32912/1‑43 (MQ=255)
                                                                                                                                      aGATTCAGACAGCGACTCAGAGTCAGAGAGCGACTCAGACTCAGATAGCGATTCAGAGTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCga        <  2:253448/141‑1 (MQ=11)
                                                                                                                                       gATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGCCTACACGACGCACAT‑‑CGACTAAGATTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgac       <  2:4288904/141‑1 (MQ=17)
                                                                                                                                           cGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAt                                                                                                         <  1:2135588/41‑1 (MQ=255)
                                                                                                                                           cGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCgactcag   <  1:2929138/141‑1 (MQ=255)
                                                                                                                                           cGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATACAGATTCAATGATATCTTTTTACTCAAACAGGGATTAAGACTCAGACAGAgactcag   >  2:1984622/1‑141 (MQ=17)
                                                                                                                                            |                                                                                                                                              
AGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGA  >  USA300TCH1516_ALE/618574‑618854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: