BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	622,359	T→C	6.8%	D974D (GAT→GAC)	sdrE →	Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	622,359	0	T	C	6.8%	1063.7 / 23.1	429	D974D (GAT→GAC)	sdrE	Serine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand): ref base T (201/183); new base C (23/11); total (231/198)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 475 total aligned reads displayed.

GATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTC  >  USA300TCH1516_ALE/622219‑622499

 acttCACACTTGACAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACtc‑agatt                                                                                                                                             <  1:719232/139‑1 (MQ=255)

    tCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACtc‑agattcag                                                                                                                                          >  1:2971708/1‑141 (MQ=255)

    tCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACtc‑agattcag                                                                                                                                          >  1:3686658/1‑141 (MQ=255)

      acacTTGATAACGGATACTTCGAATGACATACAACAGACAGCGATTCAGACTCAGATAGTGACTCAGACATCGACTCTGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCCGACAGCGACtc‑agactccgat                                                                                                                                        >  2:1823542/1‑141 (MQ=25)

      acacTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAGTAAGACACCG‑AT‑ACAGATTCAGACAGCGACta‑cgattcagat                                                                                                                                        >  2:43822/1‑141 (MQ=37)

      acacTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TAAGATTCAGACAGCGACtc‑agattcagat                                                                                                                                        >  1:127247/1‑141 (MQ=255)

         cTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGACGCAGACAGc                                                                                                                                     >  2:1705202/1‑141 (MQ=255)

          ttGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCg                                                                                                                                    >  1:515497/1‑141 (MQ=255)

           tGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGa                                                                                                                                   >  1:979534/1‑141 (MQ=255)

           tGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGa                                                                                                                                   >  2:2948882/1‑141 (MQ=255)

              tAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTAAATAAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTc                                                                                                                                <  1:3222094/141‑1 (MQ=255)

                ccGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAg                                                                                                                              <  1:1941657/140‑1 (MQ=255)

                aCGGATATCTGGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAg                                                                                                                              <  2:281682/141‑1 (MQ=255)

                aCGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAg                                                                                                                              <  2:2218091/141‑1 (MQ=255)

                   gATACTTCGAAGAAGAGACAGCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCG‑AT‑TCAGACTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGact                                                                                                                           >  2:2717938/1‑139 (MQ=255)

                   gATACTCCGAAGAAGATACATCAGACGGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGAtt                                                                                                                           <  1:1135168/141‑1 (MQ=255)

                    aTACTACGAAGAAGATACATCAGACAGCGATGCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTc                                                                                                                          <  2:3719327/141‑1 (MQ=255)

                     tACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCg                                                                                                                         >  1:993798/1‑141 (MQ=255)

                     tACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGACTCg                                                                                                                         >  1:2785651/1‑141 (MQ=255)

                      aCTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCgg                                                                                                                        <  1:4167280/141‑1 (MQ=255)

                         tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACGCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACa                                                                                                                     <  1:2001317/141‑1 (MQ=255)

                          cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATCGCGACTGCGATTCGGACAg                                                                                                                    >  2:3211636/1‑141 (MQ=255)

                          cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                    <  1:2644226/141‑1 (MQ=255)

                           gaagaaGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTcagccagc                                                                                                                   >  2:2317296/1‑141 (MQ=255)

                           gaagaaGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTAAGACAGc                                                                                                                   >  2:1246462/1‑141 (MQ=255)

                             agaagaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                                 <  1:3116354/141‑1 (MQ=255)

                              gaagaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                                                                                <  1:4220133/141‑1 (MQ=255)

                                agaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                                                              >  1:3296989/1‑141 (MQ=255)

                                 gaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                                                             >  1:327018/1‑141 (MQ=255)

                                  aTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                                                            >  1:3740604/1‑141 (MQ=255)

                                  aTACATCAGACAGCGATTCAGACTCAGATAGAGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCAGACAGGGATACaa                                                                                                            >  2:3833229/1‑140 (MQ=18)

                                       tCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                                                                       <  1:2426018/141‑1 (MQ=255)

                                       tCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                                                                       <  1:2948518/141‑1 (MQ=255)

                                       acAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                                                                       <  2:1627341/140‑1 (MQ=255)

                                        cAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                                                                      <  1:1608267/141‑1 (MQ=255)

                                          gACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGAG‑AT‑TCAGATTCAGACAGCGACTC‑CGACTCAGATAGCGACACAGACTCAGACAGCGATCCAGCCTCACAt                                                                                                    >  2:581008/1‑141 (MQ=17)

                                            cAGCGATTCAGACTCAGAGAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg                                                                                                  <  1:1098601/141‑1 (MQ=14)

                                              gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                                                                >  1:2318035/1‑141 (MQ=255)

                                                   tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca                                                                                           >  1:4413215/1‑141 (MQ=255)

                                                   tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca                                                                                           <  1:2146700/141‑1 (MQ=255)

                                                   tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca                                                                                           <  1:3555055/141‑1 (MQ=255)

                                                   tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtca                                                                                           <  1:2174497/141‑1 (MQ=255)

                                                   tCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGAATCAGACTCAGACAGTGCATATAAAATTTA‑A‑AGCGAGTA‑AGATTCAGATAGCGACTAAGAATCAGCCAGAGACGCAGACTCAGATAGCGCCtaa                                                                                           >  2:2170797/1‑139 (MQ=12)

                                                     aGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                         <  1:1402273/141‑1 (MQ=255)

                                                      gACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat                                                                                        >  1:2869095/1‑141 (MQ=255)

                                                        cTCAGATAGTGACTCAGACAGCAACTCAGACTCAAACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattc                                                                                      <  2:4178493/141‑1 (MQ=255)

                                                          cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TTAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagcttccg                                                                                    >  1:3366338/1‑139 (MQ=255)

                                                          cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCTGATAGCGACtcagattcag                                                                                    >  1:2474768/1‑141 (MQ=255)

                                                          cAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcag                                                                                    >  1:3516899/1‑141 (MQ=255)

                                                           aGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AC‑TCAGACTCAGACAGCGACTC‑AGACGCAGAAAGCGACACAGACACTGAAACCAATTCACACTCGAACAGCGACtcagacgcgga                                                                                   >  2:2094970/1‑135 (MQ=2)

                                                           aGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtgagatacagt                                                                                   >  2:2998839/1‑140 (MQ=17)

                                                           aGATAGTGACTCAGACAGCGACTCAGACACAGACTTCTTCTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                   <  1:1876068/141‑1 (MQ=17)

                                                            gataaggacacagacagcgacgcagactcagaaAGCGACTCAGACTCAGACAGTG‑AG‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                                  <  2:3137921‑M2/108‑1 (MQ=255)

                                                            gATAGTGACTCACACAGCGAATCAGACTGAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcagat                                                                                  <  2:3616590/141‑1 (MQ=255)

                                                              tAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  1:4064668/141‑1 (MQ=255)

                                                              tAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                >  1:564033/1‑141 (MQ=255)

                                                              tAGTGACTCAGACAGCGACTCAGACGCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  1:1038638/141‑1 (MQ=255)

                                                               aGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGc                                                                               <  1:2220612/141‑1 (MQ=255)

                                                                gTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGACTCAGACAGCGACTC‑AGACTCAGATAGCGACTCAGACACGGACAGCGACGCAGACCCAGATAGCGACtccgatacagaaacaa                                                                              >  2:3366775/1‑136 (MQ=14)

                                                                    ctcagacagcgactcagactcagacagcgactcagaCTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGACAGCgctgc                                                                          >  2:3417693/1‑137 (MQ=255)

                                                                      cagacagcgactcagactcagacagcgactcagactCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcag                                                                        <  1:1895788/141‑1 (MQ=255)

                                                                         acagcgactcagacttagataatcacgcacactcagACAGCG‑AT‑GCAGATTCAGACAGCGACTC‑AGACGCAGATAGCGACTCAGATACAGACAGCGATCCAGACTCAGATAGCGACTAAGATGCAGCTAGCGAGccagacg                                                                     >  2:2033718/1‑140 (MQ=2)

                                                                         acagcgactcagactcagacagcgactcagactcagACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                                 >  1:1714071/1‑97 (MQ=255)

                                                                                    gactcagacagcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGAc                                                          <  1:3860855/141‑1 (MQ=255)

                                                                                        cagacagcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcag                                                      >  1:1416767/1‑141 (MQ=255)

                                                                                         agacagcgactaagacgcagacagGG‑AT‑TCAAAGTCAGAAAGCGACTT‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcaga                                                     <  2:788965/141‑1 (MQ=14)

                                                                                          gacagcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagat                                                    >  1:4007331/1‑141 (MQ=255)

                                                                                             agcgactcaggctcagacagTG‑AT‑TCAGATTCAGAAAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca                                                 <  2:260016/141‑1 (MQ=255)

                                                                                             agcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcggattca                                                 >  1:4304159/1‑141 (MQ=255)

                                                                                             agcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca                                                 >  1:1581432/1‑141 (MQ=255)

                                                                                             agcgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattca                                                 <  1:1792255/141‑1 (MQ=255)

                                                                                               cgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGCCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattcaga                                               <  1:4366122/141‑1 (MQ=255)

                                                                                               cgactcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGATTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattcaga                                               >  1:635038/1‑141 (MQ=255)

                                                                                                  ctcagactcagaaagGG‑AT‑TCAGATTCAGACAGCGGCCC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAg                                            <  1:2384891/141‑1 (MQ=14)

                                                                                                   tcagactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                           >  1:120970/1‑141 (MQ=255)

                                                                                                     ggactcatgcagGC‑AT‑TTAGATACAGACACGGAATG‑AGATTGAGTTAGCGATTCAGATTCGTACATCGATTCAGACTCATATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                         <  2:3266522/140‑1 (MQ=14)

                                                                                                     agactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGCATCAGAGAGCGACTCAGATACGGACAGCGACTCAGACTCAGATAGCGACTCAGACACAGCGAGCGAGTCAGACTCAGAGAACGACTCACATTGAGAGAGCGa                                         >  2:4306925/1‑141 (MQ=11)

                                                                                                      gactcagacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                        <  1:1537461/141‑1 (MQ=255)

                                                                                                          gagacagTG‑AT‑TCAGATTCAGACAGAG‑CTCTATATTCAGATAGCTACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                    <  2:3301046/140‑1 (MQ=17)

                                                                                                            gacagTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCTGATTTGGGTAGCCACGCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                  >  1:2085220/1‑141 (MQ=17)

                                                                                                               agTG‑AA‑TCAGATTCAGACAGAGACTC‑AGATTCAGACAGCGAAGAAGATTAAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                               <  2:1333984/141‑1 (MQ=14)

                                                                                                                 tG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                             <  1:2265207/141‑1 (MQ=255)

                                                                                                                  g‑at‑tcagattcaGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGaa                            >  1:493566/1‑140 (MQ=255)

                                                                                                                  g‑at‑tcagattcaGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAc                            <  1:3821125/141‑1 (MQ=255)

                                                                                                                  g‑at‑tcagattcaGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTAAGATAGCGATTCGGACGCAgta                            >  2:145552/1‑139 (MQ=255)

                                                                                                                     t‑tcagattcagaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg                          <  1:1814119/141‑1 (MQ=255)

                                                                                                                       tcagattcagaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACACCGACAGCGACTCAGATTCAGATAGCCATTCCGACTCTGACacc                         >  2:1510957/1‑139 (MQ=14)

                                                                                                                          gtatcagaCAGCGACTC‑AGACTCAGATAGCGACTGAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                      <  2:4007331/138‑1 (MQ=255)

                                                                                                                            ttcagaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                    >  1:2538723/1‑141 (MQ=255)

                                                                                                                               agaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACtcag                                                                                          >  2:1802752/1‑68 (MQ=255)

                                                                                                                                gaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                >  1:3161119/1‑141 (MQ=255)

                                                                                                                                gaCAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                <  1:76320/141‑1 (MQ=255)

                                                                                                                                  cAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc              <  1:1496880/141‑1 (MQ=255)

                                                                                                                                   ggCGACTC‑AGATTCAGATAGCGAATCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa             <  1:3641807/140‑1 (MQ=255)

                                                                                                                                   cgcgcCTC‑AGACGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa             <  1:4130779/140‑1 (MQ=255)

                                                                                                                                   aGCGACTC‑AGATTCAGAGAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa             <  1:670974/141‑1 (MQ=255)

                                                                                                                                     cGACTC‑CGATCCAGATATCTCCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa           <  1:918329/141‑1 (MQ=14)

                                                                                                                                      gACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAt          <  1:2709427/141‑1 (MQ=255)

                                                                                                                                            agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga     >  1:3467630/1‑141 (MQ=255)

                                                                                                                                              attcagatAGCGACTCAGCTTCGGACAGGGACACAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACAGAGACCGCGACTGAGATAGAGACAGCGAAGCGGACTGAGACAGCGACAGAGACACAGATAGCgact   >  2:2474761/1‑141 (MQ=9)

GATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTG‑AT‑TCAGATTCAGACAGCGACTC‑AGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTC  >  USA300TCH1516_ALE/622219‑622499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`