Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,464 G→A 9.5% S1009S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4640GA 9.5% 1086.0 / 25.2 483S1009S (TCG→TCAsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (211/197);  new base A (25/32);  total (238/245)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 495 total aligned reads displayed.

TCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622324‑622604
                                                                                                                                            |                                                                                                                                               
tcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCCGATAGCGACTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                                                                                                 >  1:3005158/1‑141 (MQ=255)
  agacagTGATGCAGATGCAGACAGCGACTCAGATCAAGAAAGCGACCCCGATTCCGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                               <  2:4048841/141‑1 (MQ=14)
     cagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                            >  1:1602361/1‑141 (MQ=255)
           tgctgagtcagaCAGCGACTGAGAGTCAGAGAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg                                                                                                                                      <  2:1888501/134‑1 (MQ=11)
           gtcagattcagatAGCGAGTCAGATGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAg                                                                                                                                      <  1:89894/140‑1 (MQ=255)
               gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                                  <  1:2957102/141‑1 (MQ=255)
               gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACAGCGATAGCGACTCAGACACAGATAGCCATACAGATCCAGAGAGCGac                                                                                                                                  >  2:1692702/1‑140 (MQ=11)
                     gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGAc                                                                                                                            <  1:76320/141‑1 (MQ=255)
                        cgcgcCTCAGACGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCa                                                                                                                         <  1:4130779/140‑1 (MQ=255)
                          cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                       >  1:547727/1‑141 (MQ=255)
                              tgagagtcagagATCGACGCATAGTCAGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACACAGATTAAGCTATCGATGCTGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                   <  2:4307288/139‑1 (MQ=11)
                              tcagattcagatAGCGACTCAGATTCGGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAGTCAGACGCAGATAGc                                                                                                                   >  1:3914951/1‑141 (MQ=255)
                               cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                  >  1:2461625/1‑141 (MQ=255)
                                agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCga                                                                                                                 >  1:3836181/1‑141 (MQ=255)
                                 gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                >  1:111600/1‑141 (MQ=255)
                                  attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTAAGAATGAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                                                                               >  1:1886855/1‑141 (MQ=255)
                                     cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCgactccg                                                                                                            >  2:2441724/1‑139 (MQ=255)
                                      agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCACACTCAGACCGCGACCCAGACACAAATAGCcaccccaa                                                                                                           >  2:499654/1‑136 (MQ=11)
                                         tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                        >  2:2161721/1‑141 (MQ=255)
                                         tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                        <  1:3143181/141‑1 (MQ=255)
                                          aGCGCCTCAGACTCGCACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATACAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCCGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                                                       <  2:3266215/141‑1 (MQ=14)
                                           gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGATTCAGACAGAGAGACAGATACAGATAGCGactaagactcag                                                                                                      >  2:2661414/1‑141 (MQ=14)
                                              aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAATCAGATAGCGATTCGGACTCAGACAGCGATTCAGAACCAGATAGCGAcccagactcggaga                                                                                                   >  2:3998246/1‑139 (MQ=14)
                                              aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACACAGACTCAGAGCGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGCGTCAGCGTCGGACCGCGAAACAGACTCAGATCGCGACGCAGACGCAGACa                                                                                                   >  2:1267876/1‑141 (MQ=11)
                                                    aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                             >  1:1198508/1‑141 (MQ=255)
                                                    aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                             >  1:284029/1‑141 (MQ=255)
                                                    aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGTGACTCCGACTCAGACAGCGACt                                                                                             >  1:4418438/1‑141 (MQ=255)
                                                    aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTACGACCGCGACCCAGACACAGATAGCGAATCAGACTCCGACACCGACt                                                                                             >  2:2952111/1‑141 (MQ=14)
                                                         gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAAAGAGACTCCGACACAGATAGCGATTCGGACTCAGACAGCGATGCAGACTCAGACACCGACTCCGCCTCAGCCAGCGAAtcagaa                                                                                        >  2:3843638/1‑140 (MQ=11)
                                                           cAGCGATTCAGACTCAGATAGCGCCGCAGAGTCAGATAGCGATTCAGACTCAGACAGCCACTGAGAATCAGGTAGCGATCCGGACTCATACAGCGAATAAGAATCAGATAGCGACTCAGACTCAGACAGCGACacggattc                                                                                      >  2:2238818/1‑141 (MQ=14)
                                                             gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                    >  1:3457092/1‑141 (MQ=255)
                                                             gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGAGTCGGAGTGAGACAGCGATTCAGACTCAGATAGCGACTCAGCCTTAGACAGAGACtctgagtcag                                                                                    >  2:1792186/1‑141 (MQ=14)
                                                             gCGATTCAGAATCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                               >  2:1591651/1‑82 (MQ=25)
                                                              cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagagtcaga                                                                                   >  1:2509471/1‑141 (MQ=255)
                                                              cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAAACTCAGATAGCGACTCAGACTCAGACAGCGACtcagaaacaga                                                                                   >  2:1420190/1‑141 (MQ=14)
                                                                  tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑c                                                                            >  1:3092945/1‑141 (MQ=255)
                                                                  tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑c                                                                            >  1:3808708/1‑141 (MQ=255)
                                                                  tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑c                                                                            >  1:1453189/1‑141 (MQ=255)
                                                                     gACGCAGACAGCGACGCAGATTCAGATTGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGAt                                                                         <  2:3764997/141‑1 (MQ=11)
                                                                        tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCg                                                                      >  1:3311339/1‑141 (MQ=255)
                                                                         cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCgg                                                                     >  1:3195815/1‑141 (MQ=255)
                                                                         aaGAAAGCGACTCAGATTGAGATAGCGATTCAGATGCGGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCgg                                                                     <  2:2587388/140‑1 (MQ=14)
                                                                           gacaGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGAc                                                                   <  1:1526635/138‑1 (MQ=255)
                                                                              aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCa                                                                <  1:1246462/141‑1 (MQ=255)
                                                                               gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACCGCAACAGCGACCCAAAATCAGATAG‑‑‑CGACACGGACtgca                                                               >  2:1525178/1‑138 (MQ=14)
                                                                                tgacgctgattcagagAGCGATGAAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCAGACTCAGACAGCGAGTCAGACTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGa                                                              <  2:419230/134‑1 (MQ=11)
                                                                                cGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGa                                                              <  2:2085220/141‑1 (MQ=255)
                                                                                 gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGAt                                                             >  1:2764728/1‑141 (MQ=255)
                                                                                 gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGAt                                                             >  1:2555820/1‑141 (MQ=255)
                                                                                  aCTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGAATGCAATTTTAACCCAGAGAGCTATTCAGAGTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATa                                                            <  2:947305/141‑1 (MQ=14)
                                                                                   cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAg                                                           >  1:3704948/1‑141 (MQ=255)
                                                                                       gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGAc                                                       <  1:1400815/141‑1 (MQ=255)
                                                                                       gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGAc                                                       >  1:2027402/1‑141 (MQ=255)
                                                                                       gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAG‑‑‑CGATTCAGACCCAGGAAGCGAc                                                       >  2:701145/1‑141 (MQ=14)
                                                                                       gattcagatAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACCCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGAc                                                       <  1:3534230/141‑1 (MQ=255)
                                                                                       gattcagatAGCGACTCAGATTCAGACAGCGACGCAGAATCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGAc                                                       <  2:3686658/141‑1 (MQ=14)
                                                                                        attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACt                                                      >  1:4004071/1‑141 (MQ=255)
                                                                                        attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAAAGCAATACAGACTCAGAGAGAGACTCAGACTCAGACAGCGACTCAGATTCAGACAG‑‑‑CGACTCAGACTCAGACACCGAca                                                      >  2:1353968/1‑140 (MQ=14)
                                                                                        attcagaaAGCGATTAAGACTCAGCAAACGATTCAGCTTCAGATTGCGATTCGGACTCAGACAGCGATTCCGACTCAGATAGCGACTCAGACTCAGACAGTGCTTTAGTTTCTCTTAG‑‑‑CGATTCGGACTCAGATAGCGACt                                                      <  2:3163081/141‑1 (MQ=14)
                                                                                         ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTc                                                     <  1:660844/141‑1 (MQ=255)
                                                                                         ttcagatAGCGATTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTc                                                     <  1:707354/141‑1 (MQ=255)
                                                                                          tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCa                                                    >  1:292596/1‑141 (MQ=255)
                                                                                           cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAg                                                   >  1:1717025/1‑141 (MQ=255)
                                                                                           cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAg                                                   <  1:2668294/141‑1 (MQ=255)
                                                                                            agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGa                                                  >  1:2714979/1‑141 (MQ=255)
                                                                                                aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCa                                              >  1:4245777/1‑141 (MQ=255)
                                                                                                 gCGATTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAg                                             <  1:3169491/141‑1 (MQ=255)
                                                                                                  cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGGTTTAGACGCAAACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGa                                            >  1:1023317/1‑141 (MQ=17)
                                                                                                  cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGa                                            >  1:2339138/1‑141 (MQ=255)
                                                                                                  cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGa                                            <  1:1294516/141‑1 (MQ=255)
                                                                                                   gactcagactcagacAGCGACTCATATTCAGATAGCGAGTCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                           <  2:3542064/138‑1 (MQ=255)
                                                                                                     ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                         <  1:3394905/141‑1 (MQ=255)
                                                                                                     ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                         <  1:937355/141‑1 (MQ=255)
                                                                                                     ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                         <  2:1663358/141‑1 (MQ=255)
                                                                                                         gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGGTAGCGACTCAGATTCAGACAGCGAt                                     >  1:273354/1‑141 (MQ=255)
                                                                                                          actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAG‑‑‑CGACTCCGACTCAGACAACGACACAGACACAGACAACGact                                    >  2:2842765/1‑139 (MQ=14)
                                                                                                           ctcagacAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                   <  2:120970/141‑1 (MQ=255)
                                                                                                            ccagacAGCGTCGCACACTCAGATGGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCTGACACCGTCAGCGACGCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                  <  1:1475595/140‑1 (MQ=17)
                                                                                                                 cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                             >  1:2439886/1‑141 (MQ=255)
                                                                                                                 cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                             <  1:2133483/141‑1 (MQ=255)
                                                                                                                     gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAG‑‑‑CGACTCAGACTCAGACAGCGACTCAGACTCAGACAGAGGATTAGACTCAGaa                         >  2:3028899/1‑140 (MQ=14)
                                                                                                                       ctgagattcagatAGTGATGATGATTAAGACAGGGAGTCTGTGGAAGATAGTGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       <  2:2785793/138‑1 (MQ=14)
                                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       >  1:3634120/1‑141 (MQ=255)
                                                                                                                        gcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc                      <  1:3564403/140‑1 (MQ=255)
                                                                                                                         cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                     <  1:516714/141‑1 (MQ=255)
                                                                                                                         cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCg                     >  1:2259078/1‑141 (MQ=255)
                                                                                                                         cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGAATC‑‑ATTAGATTGGTTCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                     >  1:4375733/1‑141 (MQ=17)
                                                                                                                           gattcagatAGCGATTCGGACTCATACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCATATAG‑‑‑CGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                   <  1:1789797/141‑1 (MQ=255)
                                                                                                                           gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                   <  1:581008/141‑1 (MQ=255)
                                                                                                                           gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGACTCGGACTCAGACAGCGACTCAGATTCAGACCGCAACTCACACTCAGAGCGCgac                   >  2:610886/1‑141 (MQ=14)
                                                                                                                             ttcagacAGCGATTCGGACCCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                 <  1:2170797/141‑1 (MQ=255)
                                                                                                                                agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga              >  1:1642786/1‑141 (MQ=255)
                                                                                                                                 gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac             <  1:2418474/141‑1 (MQ=255)
                                                                                                                                   tAGCTATTCTGACTCAGATAGTGTCGCGGCCTCAGATAGCGACTCAGAGTCAGACAGCCACTCAGATTCAGATAG‑‑‑CGATTCGGTCTCAGATAGCGACTCAGAATCAGACAGCGATTCAGACTCAGATAGCgactcagactc           <  2:1133370/141‑1 (MQ=14)
                                                                                                                                    aGCGATTCGGACTCAGACAACTATTCAGACACAGATTGAGAGTAAGACTCAGACAGCGACCCATATACAAACAT‑‑‑CAATTCTCACTCATATAGCAACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca          >  2:764813/1‑141 (MQ=12)
                                                                                                                                     gCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGAATCAGACTCAGATAGCGACTCACATTCAGACAGAGATACAGACTGAGAAAGCgacgcagactgca         >  2:340713/1‑138 (MQ=17)
                                                                                                                                     gCGATTCGGACTCAGACAGCGATGCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCAACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag         >  1:3053429/1‑141 (MQ=255)
                                                                                                                                       gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac       <  1:3341178/141‑1 (MQ=255)
                                                                                                                                        aTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCCGACTCAGATACCGACgccggctccgacc      >  2:1062008/1‑140 (MQ=14)
                                                                                                                                           cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg   >  1:1086273/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                               
TCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAG‑‑‑CGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622324‑622604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: