Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,479 T→C 5.1% D1014D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4790TC 5.1% 1146.1 / 13.1 487D1014D (GAT→GACsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (214/221);  new base C (26/9);  total (248/239)
Fisher's exact test for biased strand distribution p-value = 4.64e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 500 total aligned reads displayed.

GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAG  >  USA300TCH1516_ALE/622339‑622619
                                                                                                                                            |                                                                                                                                            
gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                                              <  1:2957102/141‑1 (MQ=255)
gagacagaCAGCGCATCAGATTCAGATAGCGATTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                                              <  2:1168435/137‑1 (MQ=14)
 attcagaGAGAGACGCAGAGTCTGAGAGCGACTCAGATTAGGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAtt                                                                                                                                             <  2:217322/141‑1 (MQ=14)
  ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                            <  1:1130299/141‑1 (MQ=255)
  ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                            <  1:458589/141‑1 (MQ=255)
        atgCGACTGAGATTCAGATAGTGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTc                                                                                                                                      <  2:231289/139‑1 (MQ=255)
           cGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGa                                                                                                                                   >  1:3177071/1‑141 (MQ=255)
               tgagagtcagagATCGACGCATAGTCAGACAGCGATGCAGACTCAGATAGCGACTCAGATTCAGAGAGCGATTCAGACTCAGACAGCGACACAGATTAAGCTATCGATGCTGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                               <  2:4307288/139‑1 (MQ=11)
               tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                               >  1:3571761/1‑141 (MQ=255)
                  gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                            >  1:2529326/1‑141 (MQ=255)
                   attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTAAGAATGAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                                                                                           >  1:1886855/1‑141 (MQ=255)
                     acagatATCGACACAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactca                                                                                                                         <  1:120505/140‑1 (MQ=255)
                      cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCgactccg                                                                                                                        >  2:2441724/1‑139 (MQ=255)
                       agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCACACTCAGACCGCGACCCAGACACAAATAGCcaccccaa                                                                                                                       >  2:499654/1‑136 (MQ=11)
                          tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                                    <  1:3143181/141‑1 (MQ=255)
                          tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                                    >  1:1467859/1‑141 (MQ=255)
                           agagACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                                                                   >  1:2342310/4‑141 (MQ=255)
                           aGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                                                                                                                   <  1:686730/141‑1 (MQ=255)
                                     aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACccagccagtgtgt                                                                                                         >  1:799756/1‑136 (MQ=255)
                                     aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                                         >  1:284029/1‑141 (MQ=255)
                                     aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTGAGAGAGCGATTCAGACACAGACCGCGACGCGGAGTGCGGTCGAGATACAAACTCAAACAGCGACTCAGACCCAGATAGCGACTACGACGCAAACAGCGAcc                                                                                                         >  2:3140633/1‑140 (MQ=9)
                                     aTTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTACGACCGCGACCCAGACACAGATAGCGAATCAGACTCCGACACCGACt                                                                                                         >  2:2952111/1‑141 (MQ=14)
                                          gatggCGAGTCGGACGGAGATGTCGACTAGGAGTAAGATAGCGATTAAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                                    <  2:1364442/137‑1 (MQ=14)
                                          gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGAGTCAGATTCAGATAGCGATTCGGACTGAGACAGCGATTCAGACTCAGACAGGGTCTCCGACTCAGACCGCGACtcagaa                                                                                                    >  2:1710009/1‑140 (MQ=14)
                                          gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACACAGATAGCGACCCACCCTCAGAAAACAACtcaaac                                                                                                    >  2:2743970/1‑138 (MQ=14)
                                               cgaataaGACGCAGATAGCGACTCAGATTAAGAGAGCGATTCAGACTCAGACAGCGACTAAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                                                                                               <  2:2091536/135‑1 (MQ=14)
                                                  ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagatgg                                                                                            <  1:1114354/141‑3 (MQ=255)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                           >  1:3808708/1‑141 (MQ=255)
                                                   tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGc                                                                                           >  1:3092945/1‑141 (MQ=255)
                                                        cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                                      <  1:3459337/141‑1 (MQ=255)
                                                         tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                                     <  1:55364/141‑1 (MQ=255)
                                                         tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                                     >  1:3311339/1‑141 (MQ=255)
                                                          cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACACAGATTCAGATAGCGACTcca                                                                                    >  2:3997777/1‑139 (MQ=14)
                                                          cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGACTCGGACTCAGACAGCGCTTCAGCATCAAATAGCTACTCGGACTCAGACAGCGACTCAGACTCAGACAGCGAtacaa                                                                                    >  2:2653081/1‑137 (MQ=14)
                                                               aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACCCAGAAAGCGACTCAGAATCAGACAGCGACTCAGACTCAGATAGCGACTCGGACGCa                                                                               >  2:2001101/1‑141 (MQ=14)
                                                                  gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                            >  1:2764728/1‑141 (MQ=255)
                                                                   aCTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGAATGCAATTTTAACCCAGAGAGCTATTCAGAGTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATa                                                                           <  2:947305/141‑1 (MQ=14)
                                                                    cttagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                          <  2:403908/138‑1 (MQ=255)
                                                                    cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                          >  1:2010901/1‑141 (MQ=255)
                                                                    cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                          >  1:3704948/1‑141 (MQ=255)
                                                                     tcagattgagatGGCGAGGTGGACGCAGACAGAGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                         <  2:1116078/141‑1 (MQ=17)
                                                                       agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                       <  1:600276/141‑1 (MQ=255)
                                                                        gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACCCAGGAAGCGAc                                                                      >  2:701145/1‑141 (MQ=14)
                                                                         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                                     >  1:994159/1‑141 (MQ=255)
                                                                         attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                                     >  1:4004071/1‑141 (MQ=255)
                                                                           tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                   >  1:292596/1‑141 (MQ=255)
                                                                            cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                  <  1:2998839/141‑1 (MQ=255)
                                                                            cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                  <  1:2668294/141‑1 (MQ=255)
                                                                            cagacAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                  <  1:722446/141‑1 (MQ=255)
                                                                             agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGa                                                                 >  1:2714979/1‑141 (MQ=255)
                                                                              gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAt                                                                >  1:1394136/1‑141 (MQ=255)
                                                                                tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTAAGACTCAGAAAGCCACTCAGACTc                                                              >  2:3704497/1‑141 (MQ=14)
                                                                                  gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGATAGCGACTCAGATTCAg                                                            >  1:2467270/1‑141 (MQ=255)
                                                                                  gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACTCAGAAAGCGACTCAGATTCaa                                                            >  2:3516787/1‑140 (MQ=255)
                                                                                   cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGGTTTAGACGCAAACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                           >  1:1023317/1‑141 (MQ=17)
                                                                                   cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                           <  1:1294516/141‑1 (MQ=255)
                                                                                    gATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGATAGCGACTCAGATTCAGAc                                                          <  1:1636542/141‑1 (MQ=255)
                                                                                      ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                        >  1:53455/1‑141 (MQ=255)
                                                                                      ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                        <  1:3394905/141‑1 (MQ=255)
                                                                                      ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                        <  2:1663358/141‑1 (MQ=255)
                                                                                      ttCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                        <  1:928691/141‑1 (MQ=255)
                                                                                       tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGc                                                       <  1:4122053/141‑1 (MQ=255)
                                                                                        cagactcagactGCTTTTCAGATACGGATCGCGATTCAGAGACAGACAGCGTGTCAGAGTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCg                                                      <  2:1261137/141‑1 (MQ=11)
                                                                                        cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCg                                                      >  1:2418990/1‑141 (MQ=255)
                                                                                        cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAGCGATTCAGACTCAGATAGAGACCCAAATTCAGACaaga                                                      >  2:256686/1‑138 (MQ=14)
                                                                                          gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGGTAGCGACTCAGATTCAGACAGCGAt                                                    >  1:273354/1‑141 (MQ=255)
                                                                                          gactcagaaAGCGACAGCGACTCAGAGTGCGACTGTGACTCAGACAGAGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                    <  2:330300/141‑1 (MQ=11)
                                                                                           actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGacc                                                   >  2:1424724/1‑139 (MQ=255)
                                                                                           actcagacAGCGACTCAGAGTCAGATGGTGTCTTAGAGTCAGAGAGCGATTCAGACTGAGATAGCGAGTGAGACGCGGAGGGCGACTCAGAATGAGATAGTGACGCAGAGTGAGATAGCGACTCAGAGTCAGACTGCGagt                                                   >  2:1822861/1‑139 (MQ=2)
                                                                                            ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                  <  1:1427920/141‑1 (MQ=255)
                                                                                               agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                               >  1:3538084/1‑141 (MQ=255)
                                                                                               agacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                               >  1:3935691/1‑141 (MQ=255)
                                                                                               agacAGCGACTCAGAGTCAGATAGCGAGTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                               <  1:1724915/141‑1 (MQ=255)
                                                                                                gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                              <  1:4189566/141‑1 (MQ=255)
                                                                                                  cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGCCAGCGATTCAGACTc                                            >  1:2444971/1‑141 (MQ=255)
                                                                                                  cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                            <  1:2133483/141‑1 (MQ=255)
                                                                                                  cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCACATTCAGAAAGAGACGCCGACTc                                            >  2:3655174/1‑141 (MQ=11)
                                                                                                      gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGAtagcgactcagactcagatagcgactcagactcagaa                                        >  2:3183104/1‑140 (MQ=14)
                                                                                                        cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                      >  1:3634120/1‑141 (MQ=255)
                                                                                                         tcagattcagatAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACCCAGACAGCGACTCAGACTCAGACAga                                     >  2:1546915/1‑140 (MQ=11)
                                                                                                          cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCg                                    >  1:2259078/1‑141 (MQ=255)
                                                                                                            gattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGACTCAGATTCAGACCGCAACTCACACTCAGAGCGCgac                                  >  2:610886/1‑141 (MQ=14)
                                                                                                              ttcagacAGCGATTCGGACCCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                <  1:2170797/141‑1 (MQ=255)
                                                                                                                  gatAGCGAGTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                            <  1:4363478/141‑1 (MQ=255)
                                                                                                                     aGCGATTCGGACTCAGACAACTATTCAGACACAGATTGAGAGTAAGACTCAGACAGCGACCCATATACAAACATCAATTCTCACTCATATAGCAACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactca                         >  2:764813/1‑141 (MQ=12)
                                                                                                                      gCGATTCGGACTCAGACAGCGATGCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCAACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                        >  1:3053429/1‑141 (MQ=255)
                                                                                                                        gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                      <  1:3341178/141‑1 (MQ=255)
                                                                                                                        gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAATCAGATTCAGATAGCGACTCGGACTCCGATAGCCACGCAGACCAAGACTACGAGTCAGACACGGGACGCgactcagacagcgag                      >  2:3803654/1‑135 (MQ=9)
                                                                                                                          atCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAACGACTCAGACTCAGATAGCGACCCAGACTCACACAGCGACTCAGACCCAGATAGCAACTCAGACCCCGACAg                    >  2:278948/2‑141 (MQ=14)
                                                                                                                             gtaCTCATGCAGCGATTGAGATGCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGTTACTCAGACTCAGACAGTga                 <  1:1789988/139‑1 (MQ=17)
                                                                                                                              gACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgat                <  1:1599161/141‑1 (MQ=255)
                                                                                                                                  cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag            >  1:1702131/1‑141 (MQ=255)
                                                                                                                                  cAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcag            >  2:4180859/1‑141 (MQ=255)
                                                                                                                                    gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat          <  1:3843638/141‑1 (MQ=255)
                                                                                                                                    caCAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAGAACGCGCCTCAGACTCAGACAGTgattcagat          <  1:1254549/140‑1 (MQ=17)
                                                                                                                                      cAGCGATTCAGACTCAGATTGCTACTCATACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCCATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagaatc        >  2:3861211/1‑141 (MQ=17)
                                                                                                                                       aGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACCGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagatgca       >  1:2558531/1‑141 (MQ=255)
                                                                                                                                       aGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGAGAATCAGACTCAGATAGCGACTCAGACACAGACAATgagtcagacacc       >  2:2855109/1‑137 (MQ=14)
                                                                                                                                          gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAc    <  1:1509810/141‑1 (MQ=255)
                                                                                                                                            ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAg  >  1:3253339/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAG  >  USA300TCH1516_ALE/622339‑622619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: