Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,536 G→A 6.7% S1033S (TCG→TCA sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5360GA 6.7% 1203.5 / 6.4 499S1033S (TCG→TCAsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (221/204);  new base A (35/16);  total (264/235)
Fisher's exact test for biased strand distribution p-value = 2.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

Only 100 of 508 total aligned reads displayed.

TCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGA  >  USA300TCH1516_ALE/622396‑622676
                                                                                                                                             |                                                                                                                                            
  aGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                            >  1:3127136/1‑141 (MQ=255)
   gATAGCGACTCAGATAACGAAATCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                                           <  1:2759488/141‑1 (MQ=14)
      aGCGACTCAGATTCAGATAGCGATTCAGACTTTTTTAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGAGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCGGACGCa                                                                                                                                        >  2:2024423/1‑141 (MQ=14)
      aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGAGATTCAGACTCAGATAGCCACTCCGACTCAGCCAGCGACTCCAACTCAGACAGCGACTCAGAccaa                                                                                                                                        >  2:2711045/1‑138 (MQ=11)
       gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGACACAGAGAGCGAATCAGACGCaa                                                                                                                                       >  2:739445/1‑140 (MQ=14)
         gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                                     >  1:4188065/1‑141 (MQ=255)
         gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCACATTCAGATAGCGATTCGGAcacgaat                                                                                                                                     >  1:404536/1‑135 (MQ=255)
          acccagagtcagatAGCGACTAAGACGAAGAAAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACCGAGACTCAGAGTCAGATAGCGATTCGGACTCAGATa                                                                                                                                    <  2:2457945/138‑1 (MQ=14)
           cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGagag                                                                                                                                   >  1:2434733/1‑141 (MQ=255)
           cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                                   >  1:1133370/1‑141 (MQ=255)
           cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                                   <  1:26849/141‑1 (MQ=255)
            tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACGCAGACAGCGACTCAGATTCAGCTACCGGCTCGGACGGAGATAga                                                                                                                                  >  1:1797771/1‑140 (MQ=14)
               gattcagatAGCGATGCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACCCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                               <  1:3534230/141‑1 (MQ=255)
                attcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt                                                                                                                              >  1:2459520/1‑141 (MQ=255)
                 ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                                                                             <  1:1114979/141‑1 (MQ=255)
                 ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                                                                             <  1:660844/141‑1 (MQ=255)
                  tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                                                                            >  1:292596/1‑141 (MQ=255)
                       tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTc                                                                                                                       >  1:1767367/1‑141 (MQ=255)
                       tAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTAAGACTCAGAAAGCCACTCAGACTc                                                                                                                       >  2:3704497/1‑141 (MQ=14)
                       tAGCGATTCAGACTCAGACAGCGACTCACATTCAGATAGCGATTCAGACTCAAACAGCGATTCAGACCCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATCGCGACTCGCACCCAGATAGCGCCTCaaacac                                                                                                                       >  2:642332/1‑136 (MQ=14)
                          cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGGTTTAGACGCAAACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                                    >  1:1023317/1‑141 (MQ=17)
                          cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTGAGATAGCGATTCGGAGTCAGATAGCGACTCAGATTCAGa                                                                                                                    >  1:2557990/1‑141 (MQ=255)
                          cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                                                                                    >  1:2339138/1‑141 (MQ=255)
                           gactcagactcagacAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                                                                                                   <  1:3836468/138‑1 (MQ=255)
                           gATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGTACTCAGATAGCGACTCAGATTCAGAc                                                                                                                   <  1:1636542/141‑1 (MQ=255)
                           gATTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGAGATTCAGACTCAGATAGCGACTCAGacaccgac                                                                                                                   >  2:974818/1‑134 (MQ=14)
                             ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                                 <  1:937355/141‑1 (MQ=255)
                             ttCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAg                                                                                                                 >  1:53455/1‑141 (MQ=255)
                               cagactcagactGCTTTTCAGATACGGATCGCGATTCAGAGACAGACAGCGTGTCAGAGTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCg                                                                                                               <  2:1261137/141‑1 (MQ=11)
                               cagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAGCGATTCAGACTCAGATAGAGACCCAAATTCAGACaaga                                                                                                               >  2:256686/1‑138 (MQ=14)
                                  actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                            >  1:2822068/1‑141 (MQ=255)
                                  actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGacc                                                                                                            >  2:1424724/1‑139 (MQ=255)
                                  acgcagatATCGACTCGGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                            <  2:4239930/138‑1 (MQ=14)
                                   ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                           <  1:2789816/141‑1 (MQ=255)
                                   ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                                                                           <  1:1427920/141‑1 (MQ=255)
                                    tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATCGCGACTCAGACTCAGACAGCGAATCa                                                                                                          >  2:3683151/1‑141 (MQ=255)
                                      agacAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                                                                                        <  1:3958229/141‑1 (MQ=255)
                                       gacAGCGACTAAGATTCAGATAGCGACTCTGACTCAGACTGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                                       <  2:1595477/141‑1 (MQ=17)
                                             gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGAGGATTAGACTCAGaa                                                                                                 >  2:3028899/1‑140 (MQ=14)
                                             gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGAtagcgactcagactcagatagcgactcagactcagaa                                                                                                 >  2:3183104/1‑140 (MQ=14)
                                               cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                               >  1:3634120/1‑141 (MQ=255)
                                               agcagattcagagAGAGATGCAGACTCAGCAAGCGAGTCAGATTCAGAAAGCGACTGAGACTCAGACGGCGATTCAGATTCAGCTAGCGATTCGGACTCAGATAGCGAGTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                               <  2:1673512/139‑1 (MQ=14)
                                                tcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATCGc                                                                                              >  1:1396954/1‑141 (MQ=255)
                                                gcagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGc                                                                                              <  1:3564403/140‑1 (MQ=255)
                                                 cagattcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                                             <  1:4411416/141‑1 (MQ=255)
                                                 cagattcagatAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCg                                                                                             <  1:1462589/141‑1 (MQ=255)
                                                     ttcagatAGCGATTCGGACTCAGACAGCGATGCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                         <  1:2258629/141‑1 (MQ=255)
                                                     ttcagacAGCGATTCGGACCCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                         <  1:2170797/141‑1 (MQ=255)
                                                       cagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                       >  1:1223369/1‑141 (MQ=255)
                                                          atAGCGCTACGTGCTCAGACAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATACAGATAGCGATTCCGAACCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagact                                                                                    <  2:1539372/141‑1 (MQ=14)
                                                           aaGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGCCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                   <  1:3320651/140‑1 (MQ=255)
                                                             gCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                 >  1:3090446/1‑141 (MQ=255)
                                                              cGATGCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                                <  1:2000153/141‑1 (MQ=255)
                                                               gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGAATCAGATTCAGATAGCGACTCGGACTCCGATAGCCACGCAGACCAAGACTACGAGTCAGACACGGGACGCgactcagacagcgag                                                                               >  2:3803654/1‑135 (MQ=9)
                                                               gATTCGGACGCAGACAGCGATTGAGACTCAGATAGCGCCTCGGACACAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                               <  1:3011381/141‑1 (MQ=18)
                                                                 ttCGGACGCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACCGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                             <  1:168344/141‑1 (MQ=255)
                                                                 ttCCGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                                                                             <  1:1607570/141‑1 (MQ=255)
                                                                 atCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAACGACTCAGACTCAGATAGCGACCCAGACTCACACAGCGACTCAGACCCAGATAGCAACTCAGACCCCGACAg                                                                             >  2:278948/2‑141 (MQ=14)
                                                                    ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                          <  1:3381640/141‑1 (MQ=255)
                                                                        tCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGCATGGACATCCAGTGCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattca                                                                      <  1:2078805/141‑1 (MQ=14)
                                                                             cAGCGATTCAGACTCAGATTGCTACTCATACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCCATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagaatc                                                                 >  2:3861211/1‑141 (MQ=17)
                                                                              aGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGAGAATCAGACTCAGATAGCGACTCAGACACAGACAATgagtcagacacc                                                                >  2:2855109/1‑137 (MQ=14)
                                                                                gcaTTGAGACTCAGAGTGCGACTGAGACTCAGACAGTACCTCAGACTCAGATGGCGATTCGGACTCAGACAGAGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga                                                              <  2:3476831/139‑1 (MQ=14)
                                                                                   ttCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAg                                                           >  1:3253339/1‑141 (MQ=255)
                                                                                       gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgac                                                       >  1:708162/1‑141 (MQ=255)
                                                                                       gACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgac                                                       <  1:3728313/141‑1 (MQ=255)
                                                                                        tgccAGATGGCCACTCAGCCTCAGACAGGGAGTCAGATTCAGCTAGAGATTCCGACTCAGATAGCGACTCAGCCTCAGACAGCGAGTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgact                                                      <  2:176699/138‑1 (MQ=14)
                                                                                         tcgcgATACCGAATCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                     <  2:4004071/137‑1 (MQ=255)
                                                                                         cTCAGACATCGAAGCAGAGTCAGACAGCGACTAAGATGCAGAAAGCGATTCGGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                     <  2:2935017/141‑1 (MQ=14)
                                                                                          tCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactca                                                    <  1:85228/141‑1 (MQ=255)
                                                                                           cacaTAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcag                                                   <  1:2477037/138‑1 (MQ=255)
                                                                                            aGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTAAGACAGTGATTCAGCTTCAGACAGAgactcaaa                                                  >  2:1036766/1‑139 (MQ=255)
                                                                                                   gactcagactcaGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGAt                                           >  1:295991/1‑141 (MQ=255)
                                                                                                          actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCCGATAGCGACTCAGACTCAGACCGTGATTCAGATCCAGACAACGACACACACACAAATaaacaca                                    >  2:4140412/1‑135 (MQ=17)
                                                                                                            tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGAAAGCAACTCAGACTCAGATAGAGACTCa                                  >  2:1181336/1‑141 (MQ=255)
                                                                                                            tcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACACAGACAGCGACCCAGACTCAGATAGCGACTcc                                  >  2:2152299/1‑140 (MQ=255)
                                                                                                               gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAt                               <  1:2397433/141‑1 (MQ=255)
                                                                                                               gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAACGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAt                               >  1:1514372/1‑141 (MQ=255)
                                                                                                                 cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                             >  1:1853409/1‑141 (MQ=255)
                                                                                                                   gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                           >  1:2615866/1‑141 (MQ=255)
                                                                                                                   gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                           >  1:1718460/1‑141 (MQ=255)
                                                                                                                   gCGAATCAGATGCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCgg                           <  1:2889716/141‑1 (MQ=255)
                                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAg                       >  1:3785325/1‑141 (MQ=255)
                                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATACAGACAGCGACTCCGACTCAGCTAGCGACACAGACTCCGACAg                       >  2:3518597/1‑141 (MQ=17)
                                                                                                                          agagtcagatACCGATTGAGACTGTGATAGCGAATGAGACTCTGACAGCGAATCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCCGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGa                    <  2:1855671/141‑1 (MQ=14)
                                                                                                                             attcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAgagaga                  >  2:1235140/1‑139 (MQ=255)
                                                                                                                             attcagaaAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACt                  <  1:1527740/141‑1 (MQ=255)
                                                                                                                                cagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAg               <  1:343729/141‑1 (MQ=255)
                                                                                                                                 agatAGCGAGTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGa              <  1:2717938/141‑1 (MQ=255)
                                                                                                                                  gatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGCTTCGGACAGCGACTCacac             >  2:3578741/1‑141 (MQ=255)
                                                                                                                                   atAGCGACACCGATTAAGACAGCGCCTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGAct            <  2:436364/141‑1 (MQ=17)
                                                                                                                                    tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc           <  1:1267876/141‑1 (MQ=255)
                                                                                                                                    gaGCGAGTCGGACTCAGATAGCGTCTCTGAGTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc           <  2:53455/140‑1 (MQ=255)
                                                                                                                                     aGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActct          <  1:3169898/141‑1 (MQ=255)
                                                                                                                                     aGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActct          <  1:38824/141‑1 (MQ=255)
                                                                                                                                      gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg         <  1:1339919/141‑1 (MQ=255)
                                                                                                                                       cGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGa        <  2:2336009/141‑1 (MQ=255)
                                                                                                                                          ttCTGACTCAGATGGCGGCTCCGACTCCGACAGAGATTCAGACTCAGATAGCGACTCAGACTCAGACAGAGATTCAGACGCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg     <  2:111600/141‑1 (MQ=17)
                                                                                                                                          ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAGAGCGACTCAGACTCAGACAGTGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg     <  1:4306925/141‑1 (MQ=255)
                                                                                                                                            cGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAACGACTCAGATACGGAAAACAACTCAGAATATGATAggg   >  1:575037/1‑139 (MQ=14)
                                                                                                                                             |                                                                                                                                            
TCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCGA  >  USA300TCH1516_ALE/622396‑622676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: