BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,651	C→T	8.3%	D170D (GAC→GAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,651	0	C	T	8.3%	1216.0 / 56.5	449	D170D (GAC→GAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (154/249); new base T (18/23); total (175/274)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 450 total aligned reads displayed.

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110511‑110790

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑CATCTGACATTTTATAAAGAAGga                                                                                                                                          >  2:4143/1‑141 (MQ=17)

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGga                                                                                                                                          <  1:4362043/141‑1 (MQ=255)

   aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAAACGga                                                                                                                                          >  2:2731497/1‑141 (MQ=255)

    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGgaa                                                                                                                                         >  2:3223493/1‑140 (MQ=255)

     atatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGgaga                                                                                                                                        >  2:3670078/1‑141 (MQ=255)

             ttAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGCAACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCat                                                                                                                                >  2:4206091/1‑141 (MQ=255)

                aaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCAtata                                                                                                                             >  1:489736/1‑141 (MQ=255)

                 aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAATTTTTATAAAGACGGAGATATTTCATATaa                                                                                                                            >  2:706819/1‑141 (MQ=255)

                 aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                            >  1:1346147/1‑141 (MQ=255)

                   tGGTCGAGAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATc                                                                                                                          <  1:2029662/141‑1 (MQ=255)

                       cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                      <  1:3873328/141‑1 (MQ=255)

                         ataataAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAgg                                                                                                                    >  2:569200/1‑141 (MQ=255)

                          taataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGt                                                                                                                   >  1:4139374/1‑141 (MQ=255)

                          taataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGt                                                                                                                   <  2:3975063/141‑1 (MQ=255)

                              aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                               >  1:4268169/1‑141 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              <  1:1611408/141‑1 (MQ=255)

                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                              <  1:3595623/141‑1 (MQ=255)

                                  tCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGtt                                                                                                           <  1:1427510/141‑1 (MQ=255)

                                    aTTCCAACAAAAGAAATTAAAGCTGGATAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                         <  1:596880/141‑1 (MQ=255)

                                    aTTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAt                                                                                                         <  1:2359646/141‑1 (MQ=255)

                                      tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTc                                                                                                       >  1:782846/1‑141 (MQ=255)

                                        caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCTATCTGGTGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCCTACAATCCAGAGGTGCCGAGTTATTCgg                                                                                                     <  2:2209488/141‑1 (MQ=17)

                                         aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                    >  1:1180894/1‑141 (MQ=255)

                                          acaaAAGAAATTAAAGATGAAAAAATAAAAAACGAAATCGAAAACTTTAAGTTCTTTGTTCAATATAGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGTAGATATTTAATATAATCAAGATGTGTCGATTTATACGGCt                                                                                                   >  2:895769/1‑141 (MQ=17)

                                          acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                   <  1:4069826/141‑1 (MQ=255)

                                          acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGAAGAGTTATTCgggt                                                                                                   >  1:1683403/1‑139 (MQ=255)

                                           aaaaaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTa                                                                                                  <  2:3303825/140‑1 (MQ=255)

                                             gatctaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGACTaaa                                                                                                >  2:3947815/6‑141 (MQ=255)

                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  1:544114/141‑1 (MQ=255)

                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                               <  1:3765996/141‑1 (MQ=255)

                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGATGCAGAGTTATTCAGCTAAat                                                                                               >  2:509623/1‑141 (MQ=255)

                                               aGAAATTAAAGATTAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                              <  2:2566883/141‑1 (MQ=255)

                                                  aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                           >  1:3718441/1‑141 (MQ=255)

                                                   aatAAAGATGAAAAAATAAAACAAGAACACGCAAACTTTACGCTCCTTGCTCCCTATGGCGGCTTACAA‑AGTCTGGAAAAATATAAAGACGGAGATATTTCATATCCACAAGCGAATCCGAGTTATTCGGCTAAATATCaa                                                                                          <  2:3362160/139‑1 (MQ=12)

                                                   aTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAAGTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                          <  2:2432740/141‑1 (MQ=255)

                                                   aTTAAAGATGAAAAAATAAAAAAAGAAATAGAAACAATTAAGTACTATGTTCAATATGGCGACATTAAA‑AAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATGCAAAGGTAAAGAGTTATTCAGATTAATATGaa                                                                                          >  2:3603166/1‑141 (MQ=17)

                                                     tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                        >  1:2963365/1‑141 (MQ=255)

                                                       aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                      <  2:1849937/141‑1 (MQ=255)

                                                       aaGATGAAAAAATAAAAAAAACAATGGAAAACGTTAAGGTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                      >  1:861410/1‑141 (MQ=18)

                                                        aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                     <  1:2181308/141‑1 (MQ=255)

                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                    <  1:4017496/141‑1 (MQ=255)

                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                    <  1:4083055/141‑1 (MQ=255)

                                                         gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGATCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTAGGCGAAATATTAATTCAca                                                                                    >  2:4372312/1‑140 (MQ=17)

                                                         gATAAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATTGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                    <  2:1197394/141‑1 (MQ=255)

                                                          aagAAAAAATAAAAAAAAAAATCGAAAAATTTAAGTTTTATGAGCAATATACCAATTCCAAAGAATTTATCATAAT‑TAAAGATAGAGATATATCATATAATCCAAAGGCATTTATTTATTCGGCTAAATATCAATTAACTa                                                                                   <  2:1148773/139‑1 (MQ=2)

                                                          aTGAAAAAAAAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTGCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTa                                                                                   <  2:667509/141‑1 (MQ=255)

                                                           taaaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                  <  2:805087/139‑1 (MQ=255)

                                                           tGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                  <  2:1051202/141‑1 (MQ=255)

                                                           agAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                  <  2:456521/140‑1 (MQ=255)

                                                            gAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                 <  1:2793602/141‑1 (MQ=255)

                                                             aaaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTATGATCAATATGGCGACTTTAAA‑AATTTTAAAAATTATAAAGACGGAGATATTTCATATAATCTAAAGATACCGAGTTATTCGGATAAATATCAATTAACTAatg                                                                                >  2:3805588/1‑141 (MQ=17)

                                                              aaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAATTATCAATTAACTAatga                                                                               >  1:2890404/1‑141 (MQ=255)

                                                              aaaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                               >  1:686178/1‑141 (MQ=255)

                                                               aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                              <  2:153019/141‑1 (MQ=255)

                                                                  aTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                           >  2:2886245/1‑141 (MQ=255)

                                                                   tAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                          >  1:2406030/1‑141 (MQ=255)

                                                                            aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                 <  1:2651244/141‑1 (MQ=255)

                                                                                     aCTTTAAGTTCTTTGTTCAATATGGCGACATGAAA‑AATTGGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                        <  1:610189/141‑1 (MQ=255)

                                                                                       tttAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  2:350478/141‑1 (MQ=255)

                                                                                       tttAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  1:82224/141‑1 (MQ=255)

                                                                                       ttgaaGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                      <  1:2286121/138‑1 (MQ=255)

                                                                                        ttAAGTTCTTTGTTCAATATGTCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                     <  2:180369/141‑1 (MQ=255)

                                                                                        ttAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                     <  1:96100/141‑1 (MQ=255)

                                                                                        tgaaGTTCTGTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCa                                                     <  1:2072603/139‑1 (MQ=255)

                                                                                         tAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                    <  2:4060817/141‑1 (MQ=255)

                                                                                         tAAGTTCTTTGTTAAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                    <  2:1386543/141‑1 (MQ=255)

                                                                                               cTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGATGAATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                              >  1:2088881/1‑141 (MQ=255)

                                                                                                 ttGTTCAATATGGCGACTTTAAA‑ACTTTGAAAAATTATAACGACGGAAATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatg                                            <  1:3670078/141‑1 (MQ=255)

                                                                                                 ttGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATGATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatg                                            <  1:2581873/141‑1 (MQ=255)

                                                                                                  tGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatga                                           <  1:1781664/141‑1 (MQ=255)

                                                                                                      cAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATCAATAAAAAGTTACATTGTTACGCAAAAGATATGAtata                                       <  1:4198801/141‑1 (MQ=18)

                                                                                                       aaTATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAc                                      >  1:1844806/1‑141 (MQ=255)

                                                                                                            ggCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                 >  2:1955967/1‑141 (MQ=255)

                                                                                                                  tttAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGATATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                           >  2:3051114/1‑141 (MQ=255)

                                                                                                                     aaa‑aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                        >  1:311495/1‑141 (MQ=255)

                                                                                                                       a‑aaGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                      <  1:2501399/141‑1 (MQ=255)

                                                                                                                       a‑aaGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                      <  1:3487654/141‑1 (MQ=255)

                                                                                                                         aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                     >  2:171812/1‑141 (MQ=255)

                                                                                                                         aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                     >  1:1509427/1‑141 (MQ=255)

                                                                                                                            ttaattaaTTATAAATATGTATATATATTATATAATTTAGATGTGTTTATTTATTCGGATAAATATTAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                  <  2:2041610/135‑1 (MQ=14)

                                                                                                                            ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                  >  1:1978685/1‑141 (MQ=255)

                                                                                                                              gAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                >  1:1870927/1‑141 (MQ=255)

                                                                                                                               atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               <  1:3936328/138‑1 (MQ=255)

                                                                                                                               atgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa               >  2:389297/4‑141 (MQ=255)

                                                                                                                                 gaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAGAAATTTGATATACCGATGAATAAAGCTCCAAAattatt             >  1:1119304/2‑141 (MQ=14)

                                                                                                                                 aaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGAAAAGCTCCAAAGTTAtt             >  2:1293327/1‑141 (MQ=255)

                                                                                                                                  aaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGGTTATAATGTAAAACAATTACGCAAAAGATATGATATACCGAAGAGAAAAGATCCAAAGTTATTg            >  2:3169784/1‑141 (MQ=14)

                                                                                                                                   aTTATAAAGATGGAGATATCTAAAATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  2:3294811/141‑1 (MQ=18)

                                                                                                                                   aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTGATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  1:1011969/141‑1 (MQ=255)

                                                                                                                                   aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt           <  1:3413700/141‑1 (MQ=255)

                                                                                                                                    ttATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt          <  2:4286180/141‑1 (MQ=255)

                                                                                                                                    ttATAAAGACGGAGATATTTCATATAATACAGATGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGtt          <  2:1771458/141‑1 (MQ=255)

                                                                                                                                    atataAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGATACATATCCCTTAACTACTGATCATTATAATGTAAAACAATTACGAAAAATACATGCTATACCGCAGGGTACCGCTCCAAAGTTATTGtt          <  2:2946672/140‑1 (MQ=14)

                                                                                                                                      ataAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa        <  1:10509/141‑1 (MQ=255)

                                                                                                                                       taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  1:3218627/141‑1 (MQ=255)

                                                                                                                                       taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       >  2:1588785/1‑141 (MQ=255)

                                                                                                                                       taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  1:1952454/141‑1 (MQ=255)

                                                                                                                                       taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa       <  1:1300990/141‑1 (MQ=255)

                                                                                                                                        aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa      <  1:3524585/141‑1 (MQ=255)

                                                                                                                                           gACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATAAATTTAGTAATTGTGATTATGATATAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt   <  2:2002885/141‑1 (MQ=21)

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAA‑AATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTT  >  USA300TCH1516_ALE/110511‑110790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`