BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,028,124	G→A	69.7%	P174L (CCG→CTG)	USA300HOU_RS10230 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,028,124	0	G	A	69.7%	462.3 / 273.6	359	P174L (CCG→CTG)	USA300HOU_RS10230	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (51/57); new base A (109/140); total (161/198)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 360 total aligned reads displayed.

CGTAGATATTAACTGTATAGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATA  >  USA300TCH1516_ALE/2027984‑2028263

    gATATTAACTGTATATTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCaa                                                                                                                                         <  2:183207/141‑1 (MQ=255)

     atatTAACTGTATAGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCCTTTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTACTTAACCAGCCat                                                                                                                                        >  2:2696614/1‑141 (MQ=255)

          aaCTGTATAGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAAc                                                                                                                                   >  2:1925360/1‑141 (MQ=255)

           aCTGTATAGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAAcc                                                                                                                                  <  1:3034504/141‑1 (MQ=255)

             tGTATAGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTg                                                                                                                                <  2:1173998/141‑1 (MQ=255)

             ggTATAGCTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTg                                                                                                                                <  1:1412591/140‑1 (MQ=255)

                  aGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACtt                                                                                                                           <  1:3526509/141‑1 (MQ=255)

                  aGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACtt                                                                                                                           >  1:46329/1‑141 (MQ=255)

                     tATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTAc                                                                                                                        <  1:2534741/141‑1 (MQ=255)

                      aTCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTAcc                                                                                                                       <  1:2146690/141‑1 (MQ=255)

                      aTCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTAcc                                                                                                                       >  1:3467037/1‑141 (MQ=255)

                       tCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCt                                                                                                                      <  1:3134126/141‑1 (MQ=255)

                        ccGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTAGTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTa                                                                                                                     <  2:447445/141‑1 (MQ=255)

                        ccGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTa                                                                                                                     <  1:597629/141‑1 (MQ=255)

                         cGGTTTCATCATTTCTTCAATATGAATATCGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTaa                                                                                                                    <  1:2349126/141‑1 (MQ=255)

                          ggTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTCACAGGCAATATAATCTGCACTTTACCTcaa                                                                                                                   >  1:3909717/1‑141 (MQ=255)

                           gTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTaaaa                                                                                                                  <  2:3880499/141‑1 (MQ=255)

                            tttCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTACTTAACCAGCAATATAACCTGCACTTTACCTAAAAt                                                                                                                 >  2:1214869/1‑141 (MQ=255)

                            tttCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTACAAt                                                                                                                 >  2:2768431/1‑141 (MQ=255)

                             ttCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTACACTTTACATAAAAtg                                                                                                                >  1:1644573/1‑141 (MQ=255)

                               catcatTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAAtgtg                                                                                                              >  1:2110505/1‑141 (MQ=255)

                               catcatTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAAtgtg                                                                                                              <  2:545902/141‑1 (MQ=255)

                                 tcatTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTAACGTCAATCTCTCTACAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTc                                                                                                            <  1:1025961/141‑1 (MQ=255)

                                   atTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTa                                                                                                          <  2:2070880/141‑1 (MQ=255)

                                   atTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTa                                                                                                          <  2:1157135/141‑1 (MQ=255)

                                   atTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTa                                                                                                          <  1:1990895/141‑1 (MQ=255)

                                    tttCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTaa                                                                                                         <  1:1446170/141‑1 (MQ=255)

                                      tcgtcAATATGGATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTaaca                                                                                                       <  2:1321348/138‑1 (MQ=255)

                                       tgtcAGTAGGAATATTGTTAGTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTaacaa                                                                                                      <  1:4336411/139‑1 (MQ=255)

                                         tcAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAacaaca                                                                                                    >  2:3262636/1‑141 (MQ=255)

                                           aaTATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAATCAGCTACATGTAAATTAATATTGTAATTACACACCAAGATAACCTGCACTTTACCTAAAATGTGTCTAACAACAAt                                                                                                  >  2:3121146/1‑141 (MQ=255)

                                             tatGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATgg                                                                                                >  1:2630523/1‑141 (MQ=255)

                                                 aaTATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCCGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAAGAACGCtatt                                                                                            >  2:1508219/1‑141 (MQ=255)

                                                       gTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTc                                                                                      >  2:3424121/1‑141 (MQ=255)

                                                        ttATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTcc                                                                                     >  1:4188643/1‑141 (MQ=255)

                                                         tATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGTTATTATTTTCCt                                                                                    >  2:3590852/1‑141 (MQ=255)

                                                         tATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATTTGTCTAACAACAATGGTCTTATTTTCCt                                                                                    >  2:2395563/1‑141 (MQ=255)

                                                         tATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCt                                                                                    <  1:3683199/141‑1 (MQ=255)

                                                         tATTTTCAACTTTATATGATTTTTCACTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCATCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCt                                                                                    >  1:859691/1‑141 (MQ=255)

                                                           ttttCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTa                                                                                  <  1:64750/141‑1 (MQ=255)

                                                            tttCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTaa                                                                                 <  2:3545488/141‑1 (MQ=255)

                                                             ttCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAat                                                                                <  1:1156455/141‑1 (MQ=255)

                                                              tCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAata                                                                               <  2:1999064/141‑1 (MQ=255)

                                                                aaCTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATAtt                                                                             >  2:3938685/1‑141 (MQ=255)

                                                                aaCTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATAtt                                                                             <  1:2955981/141‑1 (MQ=255)

                                                                 aCTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATAttt                                                                            >  1:4240888/1‑141 (MQ=255)

                                                                  cTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTg                                                                           <  2:1761618/141‑1 (MQ=255)

                                                                    ttATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTGTAACAACAATGGTATTATTTTACTTAATATTTGCa                                                                         >  1:1521209/1‑141 (MQ=255)

                                                                      atatGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGc                                                                       <  2:514537/141‑1 (MQ=255)

                                                                          gATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCtttt                                                                   <  1:1236756/141‑1 (MQ=255)

                                                                          gATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTACACTTTACTTAAAATGTGTCTACCAACAATGGTATTATTTTCCTTAATATTTGCAGCtttt                                                                   >  2:724509/1‑141 (MQ=255)

                                                                           aTTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTg                                                                  >  1:787772/1‑141 (MQ=255)

                                                                           aTTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTg                                                                  >  1:3257069/1‑141 (MQ=255)

                                                                                 cattcaCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGt                                                            >  2:3740723/1‑141 (MQ=255)

                                                                                  attcaCGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTc                                                           <  1:133090/141‑1 (MQ=255)

                                                                                           aGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATTTAg                                                  >  2:3314600/1‑141 (MQ=255)

                                                                                           aGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAg                                                  >  1:677795/1‑141 (MQ=255)

                                                                                            gTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAgg                                                 <  1:1076072/141‑1 (MQ=255)

                                                                                              aattCAATAAAAAGCAGCTATATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTc                                               <  2:3992714/139‑1 (MQ=255)

                                                                                               cTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACATGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCg                                              >  1:2246576/1‑141 (MQ=255)

                                                                                                ttCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTctc                                             >  2:632478/1‑139 (MQ=255)

                                                                                                ttCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGc                                             >  1:897369/1‑141 (MQ=255)

                                                                                                 tCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAACATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAATTCAATATGTCGGTCGcc                                            >  2:3818161/1‑141 (MQ=255)

                                                                                                  ccATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCa                                           <  2:1495695/141‑1 (MQ=255)

                                                                                                     tAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTCAGTCGCCAAtt                                        >  2:2626155/1‑141 (MQ=255)

                                                                                                         aaGCCGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCAt                                    <  1:1907503/141‑1 (MQ=255)

                                                                                                         aaGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAATCCTCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCAt                                    <  2:939873/141‑1 (MQ=255)

                                                                                                         aaGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCAt                                    >  1:3799239/1‑141 (MQ=255)

                                                                                                         aaGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCAt                                    >  1:3489573/1‑141 (MQ=255)

                                                                                                          agcagcGACGTGTAAATTAATATTGTAATTAACCAGCAATATAATCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTACTATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATg                                   <  2:1363641/141‑1 (MQ=255)

                                                                                                            cagcTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGtt                                 <  2:3467037/141‑1 (MQ=255)

                                                                                                             agcTACATCTAAATTAATATACTACTTAACCATCACTATCACCGGCGCTTGACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTg                                <  1:3430844/141‑1 (MQ=255)

                                                                                                              gcTACATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGa                               <  1:3302002/141‑1 (MQ=255)

                                                                                                                  cATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATAGTATTATTTTTATTATTATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAAttt                           <  2:4075115/141‑1 (MQ=255)

                                                                                                                  cATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGa                               >  2:2459288/1‑137 (MQ=255)

                                                                                                                  cATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGa                               <  1:2459288/137‑1 (MQ=255)

                                                                                                                  cATGTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAAttt                           >  1:3860597/1‑141 (MQ=255)

                                                                                                                     gTAAATTAATATTGTAATTAACCGGCAATATACCCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTa                        <  2:2529403/141‑1 (MQ=255)

                                                                                                                     gTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTa                        >  2:2555843/1‑141 (MQ=255)

                                                                                                                     gTAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATCTGTAATTCGCCAATTCCATGTTGAATTTGTa                        <  2:79082/141‑1 (MQ=255)

                                                                                                                      tAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTAc                       <  1:2747036/141‑1 (MQ=255)

                                                                                                                      tAAATTAATATTGTAATTAACCATCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTAc                       <  2:3632322/141‑1 (MQ=255)

                                                                                                                      tAAATTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTAc                       <  2:3969999/141‑1 (MQ=255)

                                                                                                                       caaTTAATCGTGTCAGTAACCATCAATATAACCTGCACGTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACa                      <  1:1767080/140‑1 (MQ=255)

                                                                                                                       aaaTTAATATTTGTATTAACCGGTAATATAACCTGCACTTTACCTAAAATGTGTATTAGATTATTGGTATCATTTTCCTTAATATTTGCATCTCTTGTTTTGTCAATATGTAGGTCGCTAATTCCATGTTGAATTTGTACa                      <  2:2909712/141‑1 (MQ=255)

                                                                                                                       aaaTTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACa                      <  2:813217/141‑1 (MQ=255)

                                                                                                                       aaaTTAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACa                      <  1:4146297/141‑1 (MQ=255)

                                                                                                                         aTTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATc                    <  1:1828304/141‑1 (MQ=255)

                                                                                                                          tttagatTGTAATTAACCGTCAATATAACCTTCACTTTACCTAAAATGTGTCTAACAACAATTGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCt                   <  2:683867/136‑1 (MQ=255)

                                                                                                                          ttAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCt                   <  2:2485670/141‑1 (MQ=255)

                                                                                                                          ttAATATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCt                   <  1:2696614/141‑1 (MQ=255)

                                                                                                                            aaTATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTc                 >  2:1417461/1‑141 (MQ=255)

                                                                                                                            aaTATTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTATCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGTCAATTCCATTTTGAATTTGTACATCTTc                 >  2:2259417/1‑141 (MQ=255)

                                                                                                                               atTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCa              <  1:3384755/141‑1 (MQ=255)

                                                                                                                               atTGTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCa              <  1:3612278/141‑1 (MQ=255)

                                                                                                                                  gTAATTAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACtt           >  2:427046/1‑141 (MQ=255)

                                                                                                                                       tAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTtatata      <  1:968802/141‑1 (MQ=255)

                                                                                                                                       tAACCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATATTGACTCTGTACATCTTCACACTtatata      >  2:567359/1‑141 (MQ=255)

                                                                                                                                        aaCCAGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATAc     <  2:2862930/141‑1 (MQ=255)

                                                                                                                                         aCCAGCAATAGAACCTGCATTTTACCTAAAAGGGGTCTAATAACAGTGGTATTGTTTTCCTTCATACTTGCAGCTGTTGTTAAGTCAATATGTGGGTCGCCAATTCCATGTTGAATTTGTACAACTTCCCACGTATATACa    <  2:2296840/141‑1 (MQ=255)

CGTAGATATTAACTGTATAGTTATCCGGTTTCATCATTTCTTCAATATGAATATTGTTATTTTCAACTTTATATGATTTTTCATTCACGTAAGTACTTCCATAAAAAGCAGCTACATGTAAATTAATATTGTAATTAACCGGCAATATAACCTGCACTTTACCTAAAATGTGTCTAACAACAATGGTATTATTTTCCTTAATATTTGCAGCTTTTGTTAAGTCAATATGTAGGTCGCCAATTCCATGTTGAATTTGTACATCTTCCCACTTATATACATA  >  USA300TCH1516_ALE/2027984‑2028263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`