BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,124,510	C→A	7.3%	S213* (TCA→TAA)	lexA_2 →	LexA repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,124,510	0	C	A	7.3%	1077.0 / 10.6	380	S213* (TCA→TAA)	lexA_2	LexA repressor
Reads supporting (aligned to +/- strand): ref base C (211/128); new base A (3/37); total (214/166)
Fisher's exact test for biased strand distribution p-value = 8.51e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

ACCTATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAAC  >  USA300TCH1516_ALE/2124370‑2124650

 ccTATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCa                                                                                                                                             >  1:1079546/1‑141 (MQ=255)

   tATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACt                                                                                                                                           <  2:263895/141‑1 (MQ=255)

     tGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGCAGGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACtaa                                                                                                                                         >  2:1980746/1‑141 (MQ=255)

      gTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACtaaa                                                                                                                                        >  2:4126973/1‑141 (MQ=255)

        ttAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACtaaata                                                                                                                                      <  1:3963255/141‑1 (MQ=255)

        ttAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACtaaata                                                                                                                                      <  1:4102130/141‑1 (MQ=255)

           aaGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAg                                                                                                                                   >  1:1345038/1‑141 (MQ=255)

           aaGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATACTAGAAGAAAAATCGTACGTTAAGAAAGTCTTTGA‑TGAAGATGATAGATTGACTCTAGTTTCACtaaataaaa                                                                                                                                   >  2:513289/1‑140 (MQ=255)

           aaGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGCACAACATGTCAGTTGCATCATTGTAGAAGAAGCCTACGTTATGAAAATCCTTGT‑TAAAAATGATAGACTGACTTTAGTATAACCAAATAAAg                                                                                                                                   >  2:1805951/1‑141 (MQ=255)

            aGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGa                                                                                                                                  <  1:1465695/141‑1 (MQ=255)

            aGATGGAGAAATCAGAGTGGTGGAGAAAACGCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGa                                                                                                                                  <  2:2383414/141‑1 (MQ=255)

              aTGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGAtt                                                                                                                                >  1:4295237/1‑141 (MQ=255)

               tGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTACACTAAATAAAGATTa                                                                                                                               >  2:1306153/1‑141 (MQ=255)

                ggCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTac                                                                                                                              >  2:2852393/1‑141 (MQ=255)

                ggCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTac                                                                                                                              >  2:3992620/1‑141 (MQ=255)

                 gCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTaca                                                                                                                             >  2:3170835/1‑140 (MQ=255)

                    aaaGCATATTTGTGGAAAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTacgacg                                                                                                                          <  2:2058090/141‑1 (MQ=255)

                     aaTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTacaatga                                                                                                                         >  2:1495971/1‑136 (MQ=255)

                      aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGCAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAt                                                                                                                        >  1:359992/1‑141 (MQ=255)

                      aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAt                                                                                                                        >  2:1355261/1‑141 (MQ=255)

                      aTCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGAt                                                                                                                        <  1:1804619/141‑1 (MQ=255)

                        tatatTTGTGGAGAAAACCCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCt                                                                                                                      <  1:2516228/140‑1 (MQ=255)

                              tgtgGAGAAAACGCACAATATAAAGAATGGTCAAATTGGTATAGTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGACTACGACGATCTACACtt                                                                                                                <  2:1179329/141‑1 (MQ=255)

                                  gagaAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTtat                                                                                                            >  1:2929328/1‑141 (MQ=255)

                                  gagaAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTtat                                                                                                            >  2:441585/1‑141 (MQ=255)

                                    gaAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAg                                                                                                          >  1:2871739/1‑141 (MQ=255)

                                        aCTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTGTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAAt                                                                                                      >  1:2023043/1‑141 (MQ=255)

                                        aCTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAAt                                                                                                      >  1:97633/1‑141 (MQ=255)

                                           cataaTATAAAGAATGGAGAAATTTGTATATTGATCAGTGAAGAAGAAGTGTACGTTAAGAAAGTCTTTGT‑TGAAGACGATATATTTTTTTTTTTTTAATGATACGGCGACTACCGAGATCTACACTTTTATAGAAATGaa                                                                                                   <  2:2777088/138‑1 (MQ=255)

                                           cacaATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGaa                                                                                                   >  1:1315671/1‑141 (MQ=255)

                                                tataAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAgtgt                                                                                              >  2:420947/1‑141 (MQ=255)

                                                 gtaAAGAATGGAGGAATTGGGATATTCAGCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATTATAGATTGTTTTTTTTTTAATGATACGGCGACCACCGAGATCTACACTTTTATAGAAATGAAAGtgtg                                                                                             <  2:1836877/140‑1 (MQ=255)

                                                   aaaGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGCAAGTGTGAg                                                                                           >  1:1865713/1‑141 (MQ=255)

                                                   aaaGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAg                                                                                           >  2:2688730/1‑141 (MQ=255)

                                                      gAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTATGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTAACTAAATAAAGATTCAGTCCAACGACACTTTTATAGAAATGAAAGTGTGAGGtt                                                                                        <  2:3605638/141‑1 (MQ=255)

                                                       aaTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTa                                                                                       <  1:3316963/141‑1 (MQ=255)

                                                       aaTGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTGTATAGAAATGAAAGTGTGAGGTTa                                                                                       <  1:2322380/141‑1 (MQ=255)

                                                          ggACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAAtt                                                                                    <  1:3045691/141‑1 (MQ=255)

                                                          ggACAAATTGGTATATTCAGCATTTAAAAAGAAGCGTACGTTAAGAAAGTTTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTTCACTTTTATAGAAATGACAGTGTTAGGTTAAtt                                                                                    >  2:1613900/1‑141 (MQ=255)

                                                           gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                   >  1:260445/1‑141 (MQ=255)

                                                           gACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTAACTAAATAAAGATTACGGCGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                   <  2:220065/141‑1 (MQ=255)

                                                           gACAAATTGGTATATTCATCATAGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTg                                                                                   >  2:3373289/1‑141 (MQ=255)

                                                            aCAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                  >  2:3734226/1‑141 (MQ=255)

                                                            aCAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                  >  2:3426876/1‑141 (MQ=255)

                                                            aCAAATTGGGATAGTCATCATTGAAGAAGAAGCGGACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTgg                                                                                  <  1:325777/141‑1 (MQ=255)

                                                             cagaTTGATATATTCATCATTGAAGAAGAATTGTATGTTACGAAAAAATATATGTGAA‑TTGTTAGATTGTTTTTTGTTTAATGAAATAAAGATTACCGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGa                                                                                 <  2:3128456/138‑1 (MQ=255)

                                                             aaaaTTGGTATATTGATTATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATTATAGATTGATTCTTTTTTAACGAAATAGCGACTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGa                                                                                 <  2:2911179/140‑1 (MQ=255)

                                                              aaaTTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGaa                                                                                <  1:3736980/141‑1 (MQ=255)

                                                               aaTTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAACTGTTGAGGTTAATTGGaaa                                                                               >  1:2757082/1‑141 (MQ=255)

                                                               aaTTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGACGATAGCTTGACTATAGTATCACTAAATAAAGATTCAGACGATCTACACTCTTACAGACATGAAAGTGTGAGGCTACTTGCaaa                                                                               >  2:743147/1‑141 (MQ=255)

                                                                 ttGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAg                                                                             <  1:3101776/141‑1 (MQ=255)

                                                                     tataTTCATAATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGTCTTTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAt                                                                         <  2:564274/141‑1 (MQ=255)

                                                                     gttatTCATTGTTGAAGAAGATGCGTAGGTTGAGAAAGTGTTTGT‑TGAAGATGATAGATTGTCTTTTTTTTAATGAAATAGAGACCACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAt                                                                         <  2:6259/139‑1 (MQ=255)

                                                                        atTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTAtttt                                                                      <  1:121151/141‑1 (MQ=255)

                                                                            atcatTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATaa                                                                  >  2:1232830/1‑141 (MQ=255)

                                                                               atTGAATAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAg                                                               <  1:629521/141‑1 (MQ=255)

                                                                                ttGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAgg                                                              <  2:692048/141‑1 (MQ=255)

                                                                                   aagaagaagCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGc                                                           >  2:1419059/1‑141 (MQ=255)

                                                                                   aagaagaagCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGc                                                           <  1:1014532/141‑1 (MQ=255)

                                                                                     gaagaagCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCAc                                                         >  1:492350/1‑141 (MQ=255)

                                                                                       agaagCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACtt                                                       >  2:2996132/1‑141 (MQ=255)

                                                                                       agaagCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACtt                                                       >  1:1574616/1‑141 (MQ=255)

                                                                                          agCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCa                                                    <  1:2451564/141‑1 (MQ=255)

                                                                                          agCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCa                                                    >  2:3805016/1‑141 (MQ=255)

                                                                                           gCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCaa                                                   >  1:549745/1‑141 (MQ=255)

                                                                                              aaCGTTAAGAATGTCATTGT‑TGAAGATGATAGAGTGACTATAGTTTAACGAAATAAAGACTACGAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATg                                                <  2:3495417/140‑1 (MQ=255)

                                                                                                cGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaa                                              <  1:3795449/141‑1 (MQ=255)

                                                                                                cGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaa                                              >  2:1538657/1‑141 (MQ=255)

                                                                                                agTTAAGAAAGTGTTGGT‑TGAAGATGATAGATTGATTTTAGTTTAATGAAATAAAGATTACGAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaa                                              <  2:3755764/140‑1 (MQ=255)

                                                                                                 gTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaa                                             >  2:2478704/1‑141 (MQ=255)

                                                                                                 gTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaa                                             >  1:3880177/1‑141 (MQ=255)

                                                                                                  ttAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGAAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaaa                                            <  2:1079546/141‑1 (MQ=255)

                                                                                                   tAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaaaa                                           <  1:2136410/141‑1 (MQ=255)

                                                                                                    aaGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGaaaaaa                                          <  1:52859/141‑1 (MQ=255)

                                                                                                     aGAAAGTCTTTGT‑TGAAGACGATAAATTGACTCTAGGTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAat                                         <  1:1495971/141‑1 (MQ=255)

                                                                                                        aaGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATg                                      <  1:3154044/141‑1 (MQ=255)

                                                                                                          gTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGat                                    >  1:1682082/1‑141 (MQ=255)

                                                                                                             tttGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATAtt                                 >  2:837216/1‑141 (MQ=255)

                                                                                                               tgt‑tgAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGc                               <  2:2535006/141‑1 (MQ=255)

                                                                                                               tgt‑tgAAGATGATAGATTGACTCTAGTTTCACTAAAAACAGATGACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGc                               <  1:2276746/141‑1 (MQ=255)

                                                                                                                    gAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACCCATAGAGAGTAATGAAAATGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTc                           >  2:3342200/1‑141 (MQ=255)

                                                                                                                    gAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTc                           >  1:1730082/1‑141 (MQ=255)

                                                                                                                     aaGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCt                          <  1:771784/141‑1 (MQ=255)

                                                                                                                       gatgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCGGTCTTa                        >  1:572567/1‑141 (MQ=255)

                                                                                                                       gatgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTa                        >  1:4213957/1‑141 (MQ=255)

                                                                                                                        atgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAg                       <  1:3631091/141‑1 (MQ=255)

                                                                                                                        atgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAg                       <  1:3360614/141‑1 (MQ=255)

                                                                                                                         tgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTTAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGa                      <  1:2368469/141‑1 (MQ=255)

                                                                                                                         tgatAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCCCTTGCAAATGAAAAAATATGATATTGCAGTCTTAGa                      <  1:3474075/141‑1 (MQ=255)

                                                                                                                           atAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACt                    >  2:1872475/1‑141 (MQ=255)

                                                                                                                            tAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACtt                   <  1:1240750/141‑1 (MQ=255)

                                                                                                                            tAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACtc                   >  2:3852353/1‑140 (MQ=255)

                                                                                                                             aGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACttt                  >  2:906771/1‑141 (MQ=255)

                                                                                                                              gATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTCCACTTTTATAGAAATGAAAGTGTGCGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTg                 >  2:3976268/1‑141 (MQ=255)

                                                                                                                              gATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGAAGAACTTGCAAATGAAAAAATATGATATTACAGTATTAGAAttta                 >  2:3288482/1‑140 (MQ=255)

                                                                                                                                 tGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGaaa              <  1:685425/141‑1 (MQ=255)

                                                                                                                                   aCTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACt            >  1:1421402/1‑141 (MQ=255)

                                                                                                                                     tctTGTTTCACTAAATAAAGATTACGGAGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTat          <  2:213815/141‑1 (MQ=255)

                                                                                                                                       tAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatga        >  1:3904905/1‑141 (MQ=255)

                                                                                                                                         gTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTatgaat      >  1:2999032/1‑141 (MQ=255)

ACCTATGTTTAAAGATGGCGAAATCATATTTGTGGAGAAAACTCACAATATAAAGAATGGACAAATTGGTATATTCATCATTGAAGAAGAAGCGTACGTTAAGAAAGTCTTTGT‑TGAAGATGATAGATTGACTCTAGTTTCACTAAATAAAGATTACGACGATCTACACTTTTATAGAAATGAAAGTGTGAGGTTAATTGGAAAAGTTATTTTATAAAAGGAGCACTTGCAAATGAAAAAATATGATATTGCAGTCTTAGACTTTGAAACTATGAATGAAC  >  USA300TCH1516_ALE/2124370‑2124650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`