BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,855	A→G	5.4%	D777D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,855	0	A	G	5.4%	1161.4 / 8.4	503	D777D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (230/214); new base G (10/30); total (248/255)
Fisher's exact test for biased strand distribution p-value = 1.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 511 total aligned reads displayed.

GAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATC  >  USA300TCH1516_ALE/2774715‑2774995

gAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGATTCTGAGTCGCTATC‑GGAGTGTGAATCGCTTTCTGTATCTGATTCGCTATCTGaa                                                                                                                                              >  2:4255751/1‑141 (MQ=17)

gAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGaa                                                                                                                                              >  1:527454/1‑141 (MQ=255)

gAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGaa                                                                                                                                              >  1:2035691/1‑141 (MQ=255)

 aGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAAt                                                                                                                                             >  1:3644089/1‑141 (MQ=255)

  gtgagtATCTGAGTCTGAGTGGCTATCTGTATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATc                                                                                                                                            <  2:3207122/136‑1 (MQ=255)

   tCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATcc                                                                                                                                           >  1:3321240/1‑141 (MQ=255)

    taCTATTTGAGTCTGAGTGGCTATCTGAGTCCGAGTCGCTCTCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCg                                                                                                                                          <  2:2744074/140‑1 (MQ=255)

      cTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGtctgaatctgaa                                                                                                                                        >  1:420001/1‑141 (MQ=255)

              gtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGCGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATGCGAATAGCTatc                                                                                                                                >  1:50252/1‑141 (MQ=255)

                 tgagtcGCTATCTGAATCCGAGTCGCTAGCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctga                                                                                                                             <  1:1220753/141‑1 (MQ=255)

                  gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                                            >  1:233524/1‑141 (MQ=255)

                   ggtcTCTATCTGAATCCGAGTCGGTATCCGAGTCGGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaat                                                                                                                           <  1:2774785/140‑1 (MQ=255)

                    gtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGATTCCGAATCGCCgtctgaatc                                                                                                                          >  2:3672165/1‑141 (MQ=14)

                     tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCGGTCTAAGCCTGCATCACTATCCGAAGCCGAGTCGCGctctgacacc                                                                                                                         >  2:148691/1‑137 (MQ=14)

                     tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatct                                                                                                                         >  1:554360/1‑141 (MQ=255)

                       gCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATAATTGCTAGAACCCGAATCGCTatatgaatctga                                                                                                                       >  1:1863016/1‑141 (MQ=18)

                        cTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTAtctgaatctgaa                                                                                                                      >  1:1748094/1‑141 (MQ=255)

                          aTCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCtgaatctgaatc                                                                                                                    <  2:2459146/141‑1 (MQ=255)

                           tCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCg                                                                                                                   >  1:4388999/1‑141 (MQ=255)

                           tCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCg                                                                                                                   <  2:126277/141‑1 (MQ=255)

                            ttgatgccGTGTGGCTATCCGAGTCTGAGGCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                                  <  2:113496/135‑1 (MQ=17)

                             tGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCt                                                                                                                 <  1:2103867/141‑1 (MQ=255)

                                aTCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCCCtctc                                                                                                              >  2:2983672/1‑141 (MQ=255)

                                   cGAGTCGCTATCCGAGTCTGCGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGa                                                                                                           <  1:1512758/141‑1 (MQ=255)

                                    gtgTCGCTAGTCGTGTGTGAGTGGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAg                                                                                                          <  2:4366982/139‑1 (MQ=18)

                                    gAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGTCTCGCTGTCTGAGTCTGTCTCACTCTCCGaa                                                                                                          >  2:3769437/1‑140 (MQ=14)

                                     aGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGGATCTGATTCTCTCTCTGACTCTAAATCTCTATCTGAGt                                                                                                         >  2:1668223/1‑141 (MQ=14)

                                         gCTATGCGAGGCTGGGTCGCCATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGa                                                                                                     <  2:1920532/141‑1 (MQ=17)

                                         gCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGa                                                                                                     >  1:3069210/1‑141 (MQ=255)

                                         gCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTATCTGAATCTGAATCGCTATCTGAGTCTGAATCGCTATCCGAGTCTGa                                                                                                     >  2:52622/1‑141 (MQ=255)

                                             tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCTCTATCCGAGTCTGAGTCg                                                                                                 >  1:4119614/1‑141 (MQ=255)

                                              ccGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                <  1:4277252/141‑1 (MQ=255)

                                                 tgggtGAGGCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGt                                                                                             <  2:2026090/137‑1 (MQ=14)

                                                  gTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtc                                                                                            >  1:2283/1‑141 (MQ=255)

                                                      gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaa                                                                                        >  1:4031596/1‑141 (MQ=255)

                                                        gTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatc                                                                                      <  1:1145504/141‑1 (MQ=255)

                                                         tCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgcatct                                                                                     >  1:4314455/1‑141 (MQ=255)

                                                         tCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatct                                                                                     <  1:1061199/141‑1 (MQ=255)

                                                          cGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctg                                                                                    <  1:3555329/141‑1 (MQ=255)

                                                           gCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTAACACGTGT‑TGATTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctga                                                                                   <  1:3012923/141‑1 (MQ=18)

                                                              gtCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTGTC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATctg                                                                                                            <  1:1924845/112‑3 (MQ=11)

                                                              aTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCtgaatctgaatc                                                                                <  1:1336093/141‑1 (MQ=255)

                                                               tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGCATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCg                                                                               <  1:2951074/141‑1 (MQ=255)

                                                               tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATAGCTATCTTAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCg                                                                               >  1:1011603/1‑141 (MQ=255)

                                                                cTGGGTCTGAATCGCTGACTGAGTCTGAGTCGCTTTC‑CATTGGTGAGTAGGTGTTTGTATCTGAGTCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGc                                                                              <  2:3321240/141‑1 (MQ=17)

                                                                  gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGCCGCTGTCTGAATCTGAATCGCTg                                                                            >  1:2919538/1‑141 (MQ=255)

                                                                      gtGAGTCGCTGGCTGGGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTg                                                                        <  2:2377371/140‑1 (MQ=14)

                                                                       tggaTCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGa                                                                       <  2:1609244/138‑1 (MQ=255)

                                                                       tGATTCGCTGTCTGATTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGACTCTGAATCACTGTCTGAATCTGAATCGTTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGa                                                                       <  2:3080840/141‑1 (MQ=14)

                                                                         gagctcTGTCTGAGTCTGTGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                     <  2:2556140/136‑1 (MQ=255)

                                                                         aaccgcgGTCTGAGTCTGAGTCGCTAGC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGt                                                                     <  2:4306731/138‑1 (MQ=17)

                                                                         aaTCGCTGTCTGAGTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCGGAATCGCTTTCTGAATCGGAATAGCTTTCTGAATCTGAGTCGCTTTATGATTCTGAGTCGCTGTCGGAGt                                                                     >  2:3265560/1‑141 (MQ=14)

                                                                            cGCTGTCTTAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                                  <  1:3989808/141‑1 (MQ=255)

                                                                            cGCTGTCTGGGTCTGAGTCGCTATC‑TGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                                  <  2:707939/141‑1 (MQ=255)

                                                                             ggtGTCTGCGTCTGAGTCGCGATC‑GGAGTCGGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAAGCGCTGTCTGAGTCCGa                                                                 <  2:135265/139‑1 (MQ=17)

                                                                             gCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGa                                                                 >  1:3149228/1‑141 (MQ=255)

                                                                             gCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGa                                                                 >  1:891853/1‑141 (MQ=255)

                                                                             gCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGa                                                                 >  1:985152/1‑141 (MQ=255)

                                                                                   tgagtctgagtcGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCt                                                           >  1:2832152/1‑141 (MQ=255)

                                                                                    gagtctgagtcGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTa                                                          >  1:4088317/1‑141 (MQ=255)

                                                                                      gtctgagtcGCTATC‑GGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGCATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                        <  1:733272/141‑1 (MQ=255)

                                                                                      gtctgagtcGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                        <  1:3280504/141‑1 (MQ=255)

                                                                                      gtctgagtcGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                        <  1:2182534/141‑1 (MQ=255)

                                                                                        ctgagtcGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTGTCTGGATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCCGAATCGCTatcta                                                      >  2:3518835/1‑140 (MQ=14)

                                                                                            gtcGGTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCAGACTCGCGATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatc                                                  <  1:1451394/141‑1 (MQ=255)

                                                                                            gtcGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCAGAATCGCTATCTGAATCTGAATCGCTATCTGAGTGTGTATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTatctcagtc                                                  >  2:3802126/1‑136 (MQ=14)

                                                                                              cGCTATC‑CGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAGTCTGACTCGCTATCAGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGCCTGAGTACGAATCGCTatccgaatctg                                                >  2:1834581/1‑141 (MQ=17)

                                                                                              cGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatctg                                                <  1:3237485/141‑1 (MQ=255)

                                                                                               gCTACC‑CGACTCTGAGTCGCTTTCGGATTCTGAATCACTGTCTGAATATCCCTGACTATCTGAATCTCACTCGCTATCCGATTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatctga                                               <  1:839748/141‑1 (MQ=14)

                                                                                                cTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                                  >  2:1214131/1‑73 (MQ=255)

                                                                                                      tgtgTCTGAGTCTCTGTCTGAATCTGAGTTACTGTCTGATTCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCt                                         <  2:2511241/138‑1 (MQ=14)

                                                                                                       gAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                        <  1:3943329/141‑1 (MQ=255)

                                                                                                           cTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctg                                    <  1:3874927/141‑1 (MQ=255)

                                                                                                             gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCGGAGTCGCTATCTGCGTCTGAGTCGCTgtgtgag                                  >  2:2312150/1‑141 (MQ=14)

                                                                                                             gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                  >  1:1822656/1‑141 (MQ=255)

                                                                                                             gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                  >  1:3798890/1‑141 (MQ=255)

                                                                                                             gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTCTCTGAGTCCTAATCGCTCTCTGACTCTGAGTCGCTgtctgag                                  >  2:2854419/1‑141 (MQ=255)

                                                                                                              aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                 <  1:2883136/141‑1 (MQ=255)

                                                                                                                     gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                          >  1:931582/1‑141 (MQ=255)

                                                                                                                       ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc                        >  2:216170/1‑141 (MQ=255)

                                                                                                                       ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc                        <  1:3724728/141‑1 (MQ=255)

                                                                                                                           atctgagtcACTGTCTGATTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                    <  2:566326/141‑1 (MQ=255)

                                                                                                                           atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                    <  1:1622761/141‑1 (MQ=255)

                                                                                                                           atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                    >  1:1110010/1‑141 (MQ=255)

                                                                                                                                aatcGCTGTCTGAGTCTGTATCGCTATCTGAATCTGAATCGCTATGCGAGTCTGATTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat               <  2:936608/141‑1 (MQ=11)

                                                                                                                                aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat               <  1:2596948/141‑1 (MQ=255)

                                                                                                                                aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat               <  1:3533901/141‑1 (MQ=255)

                                                                                                                                 atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTTTCTGAGTCTGAGTCGCTatctgagtc              >  2:4150853/1‑141 (MQ=17)

                                                                                                                                 atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc              <  1:2474308/141‑1 (MQ=255)

                                                                                                                                 atcACTGTCTCAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc              <  1:2414117/141‑1 (MQ=255)

                                                                                                                                  gccttttCTGATGCCTAATCGCTATGTGAATCTGATTCGGTATTCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatct             <  2:1029009/135‑1 (MQ=14)

                                                                                                                                   cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg            >  2:3951488/1‑141 (MQ=255)

                                                                                                                                       gTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTACGAGTCCCATGCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc        <  1:632671/141‑1 (MQ=14)

                                                                                                                                        tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg       >  1:2746171/1‑141 (MQ=255)

                                                                                                                                        tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg       <  1:949985/141‑1 (MQ=255)

                                                                                                                                         cggAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc      <  1:346797/139‑1 (MQ=255)

                                                                                                                                         cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc      <  1:359276/141‑1 (MQ=255)

                                                                                                                                         cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCACTATCTGAATCTGAGTCGCTATCTGAGTCTGAATCACTATCTGACTCTGAgaccc      >  2:1054072/1‑137 (MQ=14)

                                                                                                                                          tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCt     <  1:2693753/141‑1 (MQ=255)

                                                                                                                                             aTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAAACGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAAACGCTATCTGAATCTGAGTCGatatt  >  2:3028250/1‑140 (MQ=255)

GAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATC‑CGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATC  >  USA300TCH1516_ALE/2774715‑2774995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`