Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,774,930 C→T 6.4% S752S (TCG→TCA clfB ← Clumping factor B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,774,9300CT 6.4% 1216.9 / 8.7 502S752S (TCG→TCAclfBClumping factor B
Reads supporting (aligned to +/- strand):  ref base C (223/215);  new base T (24/24);  total (259/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 517 total aligned reads displayed.

TCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGA  >  USA300TCH1516_ALE/2774790‑2775070
                                                                                                                                            |                                                                                                                                            
tCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTcc                                                                                                                                              >  1:1950177/1‑141 (MQ=255)
 cGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTATGCATATGAATCGCTGTCTGAGTCCg                                                                                                                                             <  1:4415950/141‑1 (MQ=255)
  ggtGTCTGCGTCTGAGTCGCGATCGGAGTCGGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAAGCGCTGTCTGAGTCCGa                                                                                                                                            <  2:135265/139‑1 (MQ=17)
  gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGa                                                                                                                                            >  1:891853/1‑141 (MQ=255)
      tctgagtctgagTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCg                                                                                                                                        >  1:2716642/1‑141 (MQ=255)
        tgagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGACTCTGAATCACCGTCTGAGTCTGAATCACt                                                                                                                                      >  2:1046162/1‑141 (MQ=17)
        tgagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCgat                                                                                                                                      >  1:2671125/1‑139 (MQ=255)
            tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCGGACTCGCTgtct                                                                                                                                  >  2:2345909/1‑141 (MQ=255)
               gagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                               >  1:1568574/1‑141 (MQ=255)
                  tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGACTCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGCCTCTCAATCGCTGTCTGACTCTGAATCGCTgtctgactct                                                                                                                            >  2:1017635/1‑141 (MQ=14)
                    gCTACCCGACTCTGAGTCGCTTTCGGATTCTGAATCACTGTCTGAATATCCCTGACTATCTGAATCTCACTCGCTATCCGATTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatctga                                                                                                                          <  1:839748/141‑1 (MQ=14)
                         ccGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGc                                                                                                                     >  1:769041/1‑141 (MQ=255)
                           gAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                                                                                                   <  1:1404910/141‑1 (MQ=255)
                           gAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                                                                                                   <  1:4023321/141‑1 (MQ=255)
                             gTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTCTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCGGAATCACTCTCTAAAACTGAGACGCTgtc                                                                                                                 >  2:3517450/1‑141 (MQ=14)
                             gTCTGAGTCGCTGTCTGAATCTGAATAACAGACAGAAACCAAATCGCTATCTGAATCTGAATCGATATCCGAGTCAGAGTCGCTGTCTGAATCTGACTCGCTCTAAGAGTCTGAATAGCAATCAGAATCTTAGTcaatcgc                                                                                                                 >  2:2455113/1‑135 (MQ=9)
                               ttgatgtgCTGTCTGAATCTGAGTCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctg                                                                                                               <  2:2989446/134‑1 (MQ=255)
                                 gAGTCGCTGTCTGAATCTGAATCTCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTGTCAGAGTCTGAGTCGCTGTCTGACTCTGAATCGCTCTCTGAGCCTGAATCGCTATCTGAATCTGAGTCGCtcgctgta                                                                                                             >  2:1616621/1‑134 (MQ=14)
                                 gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTatctcag                                                                                                             >  2:765794/1‑141 (MQ=11)
                                 gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                                                                                             >  1:3798890/1‑141 (MQ=255)
                                 gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTCTCTGAGTCCTAATCGCTCTCTGACTCTGAGTCGCTgtctgag                                                                                                             >  2:2854419/1‑141 (MQ=255)
                                  ggtggCTGTCTGAATCTGAATCACTGTCTGGATCCGAGTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                                                                            <  2:2512195/137‑1 (MQ=255)
                                  ggTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                                                                            <  1:2228920/140‑1 (MQ=255)
                                  aGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                                                                                                            <  1:418481/141‑1 (MQ=255)
                                   gTCGCTATCTGAATCTCAATCACTTTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                                                                                           <  2:3247367/141‑1 (MQ=255)
                                     cGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                                                                                         <  1:3969764/141‑1 (MQ=255)
                                       cTGTCTTAATCTGAATCACTTTCTGAATCCGAATCGCTTTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGaa                                                                                                       <  2:3790814/141‑1 (MQ=14)
                                       cTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGaa                                                                                                       >  1:261438/1‑141 (MQ=255)
                                        tGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTTAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTTAATCTGAGTCGCTGTCTGAGTCTGAAt                                                                                                      <  1:307981/141‑1 (MQ=255)
                                         gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                     >  1:931582/1‑141 (MQ=255)
                                          tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCg                                                                                                    >  2:550852/1‑141 (MQ=255)
                                           ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc                                                                                                   >  2:216170/1‑141 (MQ=255)
                                            tgaagctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                                                  <  1:2306046/141‑1 (MQ=255)
                                               atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                               >  1:3289023/1‑141 (MQ=255)
                                                  tgaatcACTGTCCGAGGCTGAGTCGCTATCTGAGTAGAAATCTGTATCAGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                            <  2:1107386/141‑1 (MQ=14)
                                                   gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCTGCGTCTGACTCGCTatctcat                                                                                           >  2:2771052/1‑138 (MQ=14)
                                                    atgcACTGTCTGAGTCCGAATCGCTATCTGAATGTTGTTCGCTATTCTGGGCTGAGTCGCTGTCTGAATCTGAATCGCTGTCGGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                          <  2:1189616/138‑1 (MQ=14)
                                                    aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                          <  1:3533901/141‑1 (MQ=255)
                                                     atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGACTCGCTGTCTGAGTCCGACTCGCTATCTGACTCTGAGTCCCTGTCTGAGTCTGCATCGCTatctgacgc                                                                                         >  2:1920010/1‑138 (MQ=17)
                                                     atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                         <  1:2474308/141‑1 (MQ=255)
                                                         cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCAGTCTGAGTCTGAATCGCTATCTGACTCTGAg                                                                                     >  1:4311793/1‑141 (MQ=255)
                                                         cTGTCTGAATCCGAATCACTAGCGGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGCATCTGAg                                                                                     >  2:3862301/1‑141 (MQ=17)
                                                           gTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                   <  1:1894605/141‑1 (MQ=255)
                                                           gTCTGAATCCGAATCGCTATCGGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                                                   <  1:356330/141‑1 (MQ=255)
                                                              tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCt                                                                                >  1:1619729/1‑141 (MQ=255)
                                                                 ttCCGATGCGCTATGGGAAGCTGAAGCGCTATCGGAGTCTGAGTTGATGTCTGAACCTGAATCGCTGTCTGATTCGGAGTCGCGATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtc                                                                             <  2:4365048/140‑1 (MQ=14)
                                                                 aTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtc                                                                             >  1:4071313/1‑141 (MQ=255)
                                                                 aTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAAACGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAAACGCTATCTGAATCTGAGTCGatatt                                                                             >  2:3028250/1‑140 (MQ=255)
                                                                     gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgag                                                                         >  1:2867071/1‑141 (MQ=255)
                                                                       aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                       <  1:663286/141‑1 (MQ=255)
                                                                         cGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctg                                                                     >  1:1018185/1‑141 (MQ=255)
                                                                          gCTATCTGTATCTGAATCGATATCTGTGTCTGAGTCGCTTTCTGAATCTGAGTCGCTATCTGCGTCTGAATCACTCTGTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctga                                                                    <  2:3587980/141‑1 (MQ=11)
                                                                          gCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGACTGAGCCAGAATTGCGATCTGAACACCAATCGCTATCTGCGTCTGAATCGATATCAGACTCTGAGGCGCTAtctgagtccgg                                                                    >  1:3026885/1‑138 (MQ=9)
                                                                           gtATCTGAATCTGGATCGTTTTCTGAGGTTTATTCGCTTTCTGACTCTGAGTCGCTGTCTGAGTGCGAATCGCTATCTGAATCGGAGTCGCTGTTTGAGTCTGAATCTCTATCTGAATCTGAGTCGCTAtctgagtctgag                                                                   <  2:413593/140‑1 (MQ=11)
                                                                             ctcctaatctgcttcGCCAGCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCTCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                 <  1:1110170/136‑1 (MQ=14)
                                                                             atctgaatctgaATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                 <  1:603589/141‑1 (MQ=255)
                                                                                ngaatgtgaagcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgct                                                              <  1:3704960/140‑1 (MQ=255)
                                                                                  aatctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgt                                                            <  1:178257/141‑1 (MQ=255)
                                                                                    tctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGATTCGCTAtctgagtctgagtcgctgtct                                                          >  1:964084/1‑141 (MQ=255)
                                                                                       gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgagttgctgtctgag                                                       >  1:540301/1‑141 (MQ=255)
                                                                                       gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgag                                                       >  1:3217382/1‑141 (MQ=255)
                                                                                       gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgag                                                       >  1:2411839/1‑141 (MQ=255)
                                                                                        aatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgcgt                                                      >  1:2980910/1‑141 (MQ=255)
                                                                                         ttgattATCCGGCTCTGAGTGGCAGTCTGACTCTGAATCGCTCTCTGTGTCTGTATCGCTATCTGAATCTGTGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                     <  2:2089017/136‑1 (MQ=14)
                                                                                          tcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtct                                                    >  1:2539059/1‑141 (MQ=255)
                                                                                            gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgccgtctgagtctga                                                  >  1:3605352/1‑141 (MQ=255)
                                                                                              tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                <  2:3289023/141‑1 (MQ=255)
                                                                                              tATCCGAGTCTGAGTCGCTGTCTGAATCTGAAGCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                <  1:1313800/141‑1 (MQ=255)
                                                                                                tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgcgtctgagtcg                                              >  1:315391/1‑141 (MQ=255)
                                                                                                 ctgAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAATCGCTATCTGAATCTGAGTCGCTGTCTTAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgc                                             <  1:2090191/139‑1 (MQ=11)
                                                                                                 ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTAtctgagtctgagtcgctatctgatcgtgagtcgc                                             >  2:3683077/1‑141 (MQ=14)
                                                                                                  cGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                            <  1:1669641/141‑1 (MQ=255)
                                                                                                  cGAGTCGGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                            <  1:38796/141‑1 (MQ=255)
                                                                                                       cTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGACCCGCTACCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGagtctgagtcgctgtctgagtctgagtcgctgtctg                                       <  1:148691/141‑1 (MQ=255)
                                                                                                          aGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatct                                                                                                                            >  2:1260237/1‑53 (MQ=255)
                                                                                                              gCTCTCTGCATGTTAATCGTGGTCTGAGTCTGAGTCGTTCGCTTAATCTGAGTCGCTGTCTGAGGCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAgtcgctgtctgagtctgagtcgctgtccgagtctga                                <  2:4052029/141‑1 (MQ=14)
                                                                                                               cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGACTCGCTATCTGAGTCTGAGTCGCTATATGAGTATGAATCGCTGTCTGAGTCTGaa                               >  2:1551224/1‑141 (MQ=14)
                                                                                                                 gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCACTGTCTGAGTCTGAGTCACTGTCTGATTGCGAGTACCTGTCTGATTCTGaacg                             >  2:4227313/1‑139 (MQ=11)
                                                                                                                 gTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATc                             <  1:216170/141‑1 (MQ=255)
                                                                                                                  gctgagtcggaatCTCTGTCTGAGTCCGAGTCGGGATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                            <  2:141043/140‑1 (MQ=14)
                                                                                                                    tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGTCTGAGTCTGAATCGCt                          <  1:3471571/141‑1 (MQ=255)
                                                                                                                    tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                          <  1:36526/141‑1 (MQ=255)
                                                                                                                    tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                          <  1:1218231/141‑1 (MQ=255)
                                                                                                                    tgaatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCt                          >  1:2947351/1‑141 (MQ=255)
                                                                                                                      aatctgaatcGCTGTCTGAGTCCGAATCGCTATATGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTTGCTATCTGAGTCTGAGTTGCTGGCTCAATCTGAGTCGCTATCTGAGTCTGAATAGCtct                        >  1:7394/1‑139 (MQ=11)
                                                                                                                       atctgtgtcGCTGTGTGAGTGCGAATCGCTATATGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATcacttgc                       <  2:1862633/141‑7 (MQ=17)
                                                                                                                       atctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                       >  1:3373643/1‑141 (MQ=255)
                                                                                                                             atcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTgtctgaatc                 >  1:1130256/1‑141 (MQ=255)
                                                                                                                               cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGACTCGCTACCGCAAGCAGATTAACTATGTGGTTCTAAAGCGCGATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTgtctgcatctg               >  2:4291868/1‑141 (MQ=2)
                                                                                                                               cGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCGGTCTGAGTCTGAATCGCTatctgagtctg               >  1:2775484/1‑141 (MQ=255)
                                                                                                                                 cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTatcttaatcttaa             >  2:2584442/1‑138 (MQ=14)
                                                                                                                                 cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg             <  1:4260379/141‑1 (MQ=255)
                                                                                                                                 cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg             <  1:1041830/141‑1 (MQ=255)
                                                                                                                                   gTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGACTCGCTCTCTGAGTCTGAGACCCTCCCTGACTCCGAATCCCTatctgaatccgaagc           >  2:1773499/1‑138 (MQ=14)
                                                                                                                                     cggAGTCTGAGTCGTTATCTGAATCTGAGTCGCTGTCTGAGTCGGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc         <  2:69958/139‑1 (MQ=255)
                                                                                                                                     cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc         >  2:3424783/1‑141 (MQ=255)
                                                                                                                                      tGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGcc        >  2:891247/1‑140 (MQ=255)
                                                                                                                                       gAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGACTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTg       >  1:3771221/1‑141 (MQ=255)
                                                                                                                                          tCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtct    >  1:2899890/1‑141 (MQ=255)
                                                                                                                                           ccGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg   <  1:2914963/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGA  >  USA300TCH1516_ALE/2774790‑2775070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: