Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP000731	6,552	+TCTCCAGGCACTTGTGCATATTCTGAAA	intergenic (+1069/‑419)	cadX → / → USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000731	= 6552	0 (0.000)	38 (0.890) +TCTCCAGGCACTTGTGCATATTCTGAAA	31/212	0.3	100%	intergenic (+1069/‑419)	cadX/USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator
	?	CP000731	6553 =	0 (0.000)					intergenic (+1070/‑418)	cadX/USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator

ATTTCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000731/6421‑6552 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCTCT  >  CP000731/6553‑6682                                                                                                                                     ||||||||||||||||||||||||||||                                                                                                                                   ATTTCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCT                                                                                                                                                             <  2:81805/135‑1  TTTCAGGTTGTGCTTTGTTCTTCATTCCATATTTTTCTAGATCAAAAATTGATAATACTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTC                                                                                                                                                            >  1:55035/1‑135  TTTCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTACTTGGGGAATAGGAACAATCCACTCTC                                                                                                                                                            >  1:94034/1‑135    TCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGTCATTTCTTGGGGAATAGGAACAATCCACTCTCCA                                                                                                                                                          <  1:106575/135‑1      AGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAAGAACAATCCACTCTCCAGG                                                                                                                                                        >  2:124102/1‑135           GTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCGACTCTCCAGGCACTT                                                                                                                                                   >  1:84553/1‑135                 TGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATATACATTTCTTTTTGAATAGGAACAATCCACTCTCCAGGCACTTGTGCAT                                                                                                                                             <  1:127484/135‑1                  GATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATATACTTTTCTTGGTGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATA                                                                                                                                            <  1:133745/135‑1                      TTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCT                                                                                                                                        >  1:128357/1‑135                       TCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTG                                                                                                                                       >  2:177891/1‑135                        CATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTTTTTTGTTTTAGTAACAATCCACTCTCCAGGCACTTGTGCATATTCTGA                                                                                                                                      <  1:117498/135‑1                           TCCATATTTTTCTAGTTCAAAAATTGATAATGCTACCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAA                                                                                                                                   >  1:170911/1‑135                            CCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGTCTCTTTAATAGACATTTCTTTGTTAATAGGAACAATCCACTCTCCAGGCACATGTGCATATTCTGAAAAT                                                                                                                                  <  1:41569/135‑1                             CATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCCTATTCTGAAAATC                                                                                                                                 >  2:69179/1‑135                             CATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTATGCAATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAAACACTTGTGCATATTCCGAAAATC                                                                                                                                 >  2:138383/1‑135                                  TTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACGATAGACTCTTTAATAGACATTTGTTGTGAAATAGGAACAATCCACTCTCCAGGCACTGGTGCATATACAGAAAATCCACCT                                                                                                                            >  2:10799/1‑135                                         GTTCAAAAATTGATAATGCTGCCGTACAACAAGCTGTGCTATATACCATAGACTCTTTAATATACATTTCTTTGTGAATATGTACAATCCATTCTTTAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTG                                                                                                                     <  1:89983/135‑1                                         GTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCATTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATCTTCTGAAAATCCACCTTTATGTG                                                                                                                     >  1:35941/1‑135                                          tcCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAAGAACAATTCACTCTCCATGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGT                                                                                                                    <  1:173615/133‑1                                               AAATTGATAATGCTGCTGTAATACCGGCTGTGCCATATACCATAGACTCTTTAATCGACATGGTCTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGC                                                                                                               <  2:131914/135‑1                                                AATTGATAATGCTGCCGTAAAACCGGCTTTGCCATATTCCATAGACTCTTTAATATACATTTCTTGTGGAATATTAAAAATCAACTATCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCC                                                                                                              <  1:89752/135‑1                                                    GATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGATTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACA                                                                                                          <  2:55035/135‑1                                                    GATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTACTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACA                                                                                                          <  2:94034/135‑1                                                       AATGCTGCCGTAAAACCGGCTGTGCCATTTACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCA                                                                                                       <  2:187758/135‑1                                                       AATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCTGGCATTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCA                                                                                                       <  1:77165/135‑1                                                                   AAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACT                                                                                           <  1:131595/135‑1                                                                        GGCTGTGCCATGTACCATACACTCTTTAATAGTCATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTTAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATAC                                                                                      <  2:45372/135‑1                                                                        GGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACGAATAC                                                                                      <  1:39378/135‑1                                                                           TGTGCCATATACCAGAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGGAACTAATACATT                                                                                   <  1:27021/135‑1                                                                                 acATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTTTATTTGTCACTCCAACATCATAACCAGTAACTAATACATTATCGCC                                                                             <  1:12859/133‑1                                                                                      cgATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTT                                                                        <  1:144961/133‑1                                                                                           GACTCTTTAATAGACATTTCTTGGAGAATAGGAACAAACAACTCTCCAGGCACTTATGAATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACAAGTAACTAATACATTATCGCCTTCTTTAAAC                                                                   >  1:88468/1‑135                                                                                              TCTTTAATAGACATTTCTTGGGGAATAGGAAAAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAATAACTAATACATTATCGCCTTCTTTAAACTTA                                                                >  1:7468/1‑135                                                                                               CTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTGACTAATACATTATCGCCTTCTTTAAACTTAT                                                               <  2:128357/135‑1                                                                                                   AATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATT                                                           <  1:177891/135‑1                                                                                                          ATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTT                                                    <  2:170911/135‑1                                                                                                            TTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTATGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGA                                                  >  1:209097/1‑135                                                                                                              CTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATAATCGCCTTCTTTAAACTAATCATTCGTACTTGATT                                                >  2:199223/1‑135                                                                                                                   gtAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACA                                           <  2:225247/133‑1                                                                                                                      ATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATA                                        >  1:19337/1‑135                                                                                                                      ATAGGAACAATCCACTCTCAAAGGATTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATA                                        <  2:84553/135‑1                                                                                                                        atGAACAATCCACTCTCCAGGCTCTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACC                                      <  2:31062/133‑1                                                                                                                        AGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTAACGCCTTCTTTAAACTTATCATTCCAACTTAATTCAACAATCCC                                      >  1:57501/1‑135                                                                                                                         GGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTTTCGCCTTCTTTATTCTTATCATTCGAACTTGATTCAACAATACCT                                     <  1:1043/135‑1                                                                                                                             CAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAA                                 >  1:95667/1‑135                                                                                                                             CAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAA                                 >  1:78666/1‑135                                                                                                                             CAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGAGTCAACAATACCTGCAA                                 >  2:10973/1‑135                                                                                                                             CAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTATCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTACACAATACCTGCAA                                 >  1:203858/1‑135                                                                                                                               ATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATTCATTATCGCCTTCTTTAAACTTATCATTCGAACTGGATTCAACAATACCTGCAAAA                               <  1:67812/135‑1                                                                                                                                       TCCAGGCACTTGTGCATATTCTGCAAATCCACCTGTATGTGTCACACCAACATCATAAACAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCTAACTTGATTCAACAATACCTGCAAAATCTATCCC                       >  1:183834/1‑135                                                                                                                                                TTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATTCATTATCGCCTTCTTTAAACTTATTATTCGAATTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCAT              <  1:69179/135‑1                                                                                                                                                TTGTGCATATGCTGAAAATCCTCCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCTAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCAT              <  2:108994/135‑1                                                                                                                                                 TGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATA             <  2:18441/135‑1                                                                                                                                                     CATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGAT         >  2:181729/1‑135                                                                                                                                                         TTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATC     >  2:50974/1‑135                                                                                                                                                            TGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGAAACAAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACAAGGAATCATAGGATAATCTCT  >  1:65523/1‑135                                                                                                                                     ||||||||||||||||||||||||||||                                                                                                                                   ATTTCAGGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000731/6421‑6552 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCTCT  >  CP000731/6553‑6682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.