Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,373,698 +T coding (78/1335 nt) pbuG ← Guanine/hypoxanthine permease PbuG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,373,6971.T95.2% 64.1 / ‑3.3 21coding (79/1335 nt)pbuGGuanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (10/10);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAAT  >  USA300TCH1516_ALE/2373565‑2373828
                                                                                                                                      |                                                                                                                                   
gCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGaaa                                                                                                                              >  1:17780/1‑141 (MQ=255)
                  ctAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAg                                                                                                            <  1:57495/141‑1 (MQ=255)
                   tagtGCAGTCGCTACAAAAATGGCACGTTGGTCCATTTGCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAACAGATGGTGGAGTTGTTAATAAAGTTGTGATACCGCCTAAGa                                                                                                           <  2:131123/138‑1 (MQ=255)
                     aTGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGAtt                                                                                                         <  1:94933/141‑1 (MQ=255)
                             gCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCtttt                                                                                                 <  1:103057/141‑1 (MQ=255)
                             gCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGACTAAGATTTCTCtttt                                                                                                 >  1:135876/1‑141 (MQ=255)
                                aCAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTaaa                                                                                              >  2:209644/1‑141 (MQ=255)
                                 cAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAg                                                                                             >  1:133650/1‑141 (MQ=255)
                                            ccTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCata                                                                                  <  2:170850/141‑1 (MQ=255)
                                              ttGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATAtt                                                                                >  2:219064/1‑141 (MQ=255)
                                                          cATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGa                                                                    <  2:683/141‑1 (MQ=255)
                                                          cATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGa                                                                    >  1:189659/1‑141 (MQ=255)
                                                            tatCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGaaa                                                                  <  1:80811/141‑1 (MQ=255)
                                                                  ctGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGttt                                                            <  1:67224/141‑1 (MQ=255)
                                                                          cgtttAACACACGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGaa                                                    <  1:105377/139‑1 (MQ=255)
                                                                                 cacCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGTTGc                                             >  2:125881/1‑141 (MQ=255)
                                                                                             ttAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTaa                                 >  1:28769/1‑141 (MQ=255)
                                                                                                aaaCTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAAt                              <  1:131276/141‑1 (MQ=255)
                                                                                                   cTTGCGGGTTAACTGCTAAAATATAGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATAcc                           <  1:71805/141‑1 (MQ=255)
                                                                                                                         ataGGCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGATTTACACGAATGGATGCTCCTTTTTTTAAATTCCCTTATTAGTTTCATATCATTCtt     >  2:95977/1‑141 (MQ=255)
                                                                                                                            ggCCATAGATTAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACCGATGCTGACAAGGCATGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTtaat  >  1:172769/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GCGAGCCTACAAATGCTGCTAATGCAGTCGCTACAAAAATGGCACCTTGGTCCATTTTCATATCTTCTGATACGCCTTTAACACCTGCTAAACTTAAAACTTGCGGGTTAACTGCTAAAATATAGGCCATAGATAAGAAAGTTGTGATACCGCCTAAGATTTCTCTTTTAAAGTTTGTTCCATATTTATCGAACTGGAAATAGTTTTTCACGAATGGATGCTCCTTTTTTTAAAATACCTTAATAGTTTAATATCATTCTTAAT  >  USA300TCH1516_ALE/2373565‑2373828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: