| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,279,705 | G→A | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,279,705 | 0 | G | A | 96.4% | 67.7 / NA | 29 | A943V (GCA→GTA) | ebh_3 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (11/16); minor base C (1/0); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCT > USA300TCH1516_ALE/2279573‑2279839 | ccGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt > 2:161169/1‑141 (MQ=255) aGTTGATTCTACTGCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt < 1:200694/141‑1 (MQ=255) tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCa < 1:24429/141‑1 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTTTTGCCTCATc > 1:159730/1‑141 (MQ=255) aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGTCGCATCa > 1:192146/1‑141 (MQ=255) ttGGTTTACTATTTACTTCGTTATCCATTGTCGGATTGTTTGTTTATGCATCTACACTTGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat < 2:115045/140‑1 (MQ=255) ggTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCCTCATCCGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCtattt > 1:138250/1‑140 (MQ=255) cATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCTGTTTATTATTTGCATAATCAGTTGCTGATGTTCCTGTTGTTTCACCTGTTTCCGAATCTCTATTATTTGCTGTTGTCg > 2:75361/1‑141 (MQ=255) ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGaa > 2:213288/1‑141 (MQ=255) tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTGGTGTTGTCGTAGAAGCg > 2:127651/1‑141 (MQ=255) tGTCGGACTGTTTGTATATGCATCAAAACTAAAATTGTGATTAGCTTGCGGTTTATCAGTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGc < 1:105084/141‑1 (MQ=255) gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGgctgct > 1:25977/1‑141 (MQ=255) ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg < 1:72667/141‑1 (MQ=255) ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg < 1:195212/141‑1 (MQ=255) tgttgATGCATCTTCACTCGGTTTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 2:141237/141‑1 (MQ=255) atatACACTAAAATTGTTATGAGCTTGCCGTTTATCAGTTGCATCATGACTTACTGATATTACAGAAGTTTCCCCTGTTGCCGCATCACTATTATTTTGTTTCCTCCAAGAAGCGGCTGCTTTGCCATTAGCTGTCGtctc < 2:217233/138‑1 (MQ=255) tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 1:215208/141‑1 (MQ=255) tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 1:197608/141‑1 (MQ=255) gTTATTAGCTTGCGGTGTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATGTGGTGTTGTCGGAGAAGAGTCTGCTTTGCCAGTAGCGGTCGTCTGAGATGCGTTAGGTTg > 1:229776/1‑141 (MQ=255) tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGATATGGTAATTGGAGCTTGACATGAAAATAACAGACTGGTATTTGGTGTTGTCGGAGAAGCGTCTGCTGTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc > 1:55743/1‑141 (MQ=255) gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTGATGCCTGGGGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg < 1:161169/141‑1 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTCGGTTGTCCCGTATTTTCTGgtg > 1:137311/1‑141 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtg > 1:174602/1‑141 (MQ=255) catcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt < 1:56659/141‑1 (MQ=255) aGTTGCTGATGTTACTGTTGTTTCGCTGTTTGTCGCTCCAAAAATTTCTTCTTTTTTTGTAGAAGCGTCTGCTTTGCCCTTAGTTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa < 1:110044/141‑1 (MQ=255) aGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTACTATTGGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGa < 1:222902/141‑1 (MQ=255) gTTGCTGATGGTACTGTTGTTTCTGCTGTTGCCGGATCAGTATTATTGGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGGCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa < 1:219362/141‑1 (MQ=255) tgtttaTGTTGTTGTTGTGTCCCCTGTGTTCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGTTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt < 2:226019/136‑1 (MQ=255) gATGTTACGTTTGTTTCATCTGTTGCCGCTTCATTATGATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt < 1:64056/141‑1 (MQ=255) | CCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCT > USA300TCH1516_ALE/2279573‑2279839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |