Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 975,902 A→G 8.6% K400R (AAA→AGA)  oatA_1 → O‑acetyltransferase OatA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE975,9020AG 8.6% 122.6 / 2.0 46K400R (AAA→AGA) oatA_1O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand):  ref base A (15/27);  new base G (2/2);  total (17/29)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

TTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTGAGAAGAAAAAGGAATCTTCCG  >  USA300TCH1516_ALE/975763‑976039
                                                                                                                                           |                                                                                                                                         
tttATATAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCaataaa                                                                                                                                          <  2:447552/141‑1 (MQ=255)
   ataGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACCATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCCTTCaataaatct                                                                                                                                       >  2:39597/1‑139 (MQ=255)
    taGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATg                                                                                                                                      >  2:656775/1‑141 (MQ=255)
          cTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAg                                                                                                                                <  1:301345/141‑1 (MQ=255)
                  aGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACattatt                                                                                                                        >  2:397824/1‑141 (MQ=255)
                     aaaGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTCTGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAACTACATTATTGga                                                                                                                     >  1:1489/1‑141 (MQ=255)
                      aagaagGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGgag                                                                                                                    <  1:465341/141‑1 (MQ=255)
                      aagaagGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGgag                                                                                                                    <  1:464860/141‑1 (MQ=255)
                           ggTATTAAAGCTTTAAATTGGCGACCTTATTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATTGTAAAGACATTATTGGAGaaaaa                                                                                                               <  2:393346/141‑1 (MQ=255)
                                   agatttATATTGGCGATCTTTTTATATATTACAATTTATAAGAATGGCAATTGTAGTAACTTTGTTAATTCCATTTATGTTGATTTTAGTATGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAg                                                                                                       <  2:445585/138‑1 (MQ=255)
                                     cTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCt                                                                                                     >  1:227504/1‑141 (MQ=255)
                                         aaaTTGGCGATCTTCTTATATACTACAATTTATAAGATTGGCAATTGTAGTAACCTTGTTAATTACATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAACACATTATTGGTGAAAAAGCGAACAGCTTTGa                                                                                                 <  2:14597/141‑1 (MQ=255)
                                          aaTTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGATAAAGACATTATCGGATGAACAGAGAATAGCTTTGAt                                                                                                >  2:451921/1‑141 (MQ=255)
                                             ttgctaCCTTCTTATATAATACAATTTATAAGAATGGGAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATccc                                                                                             <  2:506652/136‑1 (MQ=255)
                                             tgtcttccTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCAGTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGGCATTATTGGAGAAAAAGCGAATAGCTTTGATAcc                                                                                             <  1:186810/135‑1 (MQ=255)
                                             tGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATAcc                                                                                             <  1:73740/141‑1 (MQ=255)
                                             tGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATAcc                                                                                             <  1:463027/141‑1 (MQ=255)
                                                     tttttATATACCACAATTTATATGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGa                                                                                     <  2:192355/138‑1 (MQ=255)
                                                       cttATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAg                                                                                   <  1:642838/141‑1 (MQ=255)
                                                                 acaATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTAttt                                                                         >  1:435355/1‑141 (MQ=255)
                                                                 acaATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCAAATAGCTTTCATACCACAATTGCATATAATTAttt                                                                         >  2:318330/1‑141 (MQ=255)
                                                                 acaATTTATAAAAATGGCAATTGTAGTAAACTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTAttt                                                                         <  2:556734/141‑1 (MQ=255)
                                                                    aTTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAATAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACGACAATTGAAGATAATTATTTAAt                                                                      >  1:130565/1‑141 (MQ=255)
                                                                      ttATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGc                                                                    >  1:393609/1‑141 (MQ=255)
                                                                       tataAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAGATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCg                                                                   >  1:17705/1‑141 (MQ=255)
                                                                       tataAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAGATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCg                                                                   >  1:17707/1‑141 (MQ=255)
                                                                        ataAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCgg                                                                  >  1:600507/1‑141 (MQ=255)
                                                                         taAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGa                                                                 >  1:665723/1‑141 (MQ=255)
                                                                          aaGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGAGTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGAt                                                                <  2:631763/141‑1 (MQ=255)
                                                                                 gCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCa                                                         >  2:8882/1‑141 (MQ=255)
                                                                                 gCAATTGTAGGAACCGTGTTAATTACATTTATGTTGATTTTAGTAGGTGAATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCa                                                         <  2:523530/141‑1 (MQ=255)
                                                                                  cAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCaa                                                        <  1:397824/141‑1 (MQ=255)
                                                                                     ttGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTg                                                     >  2:639986/1‑141 (MQ=255)
                                                                                      tGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGa                                                    >  1:453038/1‑141 (MQ=255)
                                                                                       gtagtaACCTTGTTAATTCCATTTATGTTTATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTTGAGAAAAAGCGAATAGCTTTGATACTACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGAt                                                   <  1:497804/141‑1 (MQ=255)
                                                                                              ccTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATac                                            <  1:625214/141‑1 (MQ=255)
                                                                                               cTTGTTAATTACATTTATGTTAATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATaca                                           <  2:706779/141‑1 (MQ=255)
                                                                                                ttGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAGATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATacac                                          <  2:17705/141‑1 (MQ=255)
                                                                                                ttGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAGATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATacac                                          <  2:17707/141‑1 (MQ=255)
                                                                                                 tGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATAcaca                                         <  1:265906/141‑1 (MQ=255)
                                                                                                 tGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATAcaca                                         <  1:55226/141‑1 (MQ=255)
                                                                                                   ttAATTTCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACAtt                                       <  2:615157/141‑1 (MQ=255)
                                                                                                    tAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTg                                      <  1:212931/141‑1 (MQ=255)
                                                                                                                   tGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTg                       >  1:152108/1‑141 (MQ=255)
                                                                                                                     atattAGTAGGTTCATTCAATAAAAATTGTAAAGCGATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAAGTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTgag                     <  1:82087/138‑1 (MQ=255)
                                                                                                                     aTTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTgag                     <  1:491813/141‑1 (MQ=255)
                                                                                                                     aTTTTAGTAGGTGAATTAAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTgag                     <  2:560174/141‑1 (MQ=255)
                                                                                                                                tGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTGAGAAGAAAAAGGa          <  1:731835/141‑1 (MQ=255)
                                                                                                                                       aataaataTGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTAAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATCCACATTGATAGCTTAGTAAGAGAGAAGAAAAAGGAAACTTcc   >  2:667051/1‑141 (MQ=255)
                                                                                                                                        ataaataTGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTGAGAAGAAAAAGGAATCTTCCg  >  2:713718/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TTTATAGAAACTCCATTTAGAAAAGAAGGTATTAAAGCTTTAAATTGGCGACCTTCTTATATACCACAATTTATAAGAATGGCAATTGTAGTAACCTTGTTAATTCCATTTATGTTGATTTTAGTAGGTGCATTCAATAAATATGGTAAAGACATTATTGGAGAAAAAGCGAATAGCTTTGATACCACAATTGAAGATAATTATTTAATGCGGATAGCACCAATTGATAACATACACATTGATGGCTTAGTAAGTGAGAAGAAAAAGGAATCTTCCG  >  USA300TCH1516_ALE/975763‑976039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑