Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,744,446 G→T 11.7% P76T (CCT→ACT)  apt ← Adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,744,4460GT11.7% 132.1 / 10.2 62P76T (CCT→ACT) aptAdenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (24/28);  new base T (2/5);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-01

CTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAT  >  USA300TCH1516_ALE/1744308‑1744586
                                                                                                                                          |                                                                                                                                            
cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGCGGTAATTCCCCTTCTTTTCTACCAGGTg                                                                                                                                            >  1:79375/1‑141 (MQ=255)
cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTg                                                                                                                                            >  1:78207/1‑141 (MQ=255)
cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTATTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTGTAACAtgtg                                                                                                                                            >  1:198307/1‑141 (MQ=255)
 taataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATATCCCATATTTTCTAACAGGTGc                                                                                                                                           >  1:469452/1‑141 (MQ=255)
     aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAg                                                                                                                                       <  2:502218/141‑1 (MQ=255)
     aaaTCAACTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTACAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACATACCGAGATCTCCTGTCATCTTTTCTAACAGTTGCAAAg                                                                                                                                       <  2:460569/141‑1 (MQ=255)
      agtgagttGTAATTGAGACTCGTTGAGCTGGTTTGAGTGCGTCTTTGTGGATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAGTTCCCTTCTTTTCTAACAGTTGCAAAGc                                                                                                                                      <  2:635022/134‑1 (MQ=255)
      aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACGGCAGGAGGAAATTGCCCTTCGTTTCTAACAGGTGCAAAGc                                                                                                                                      <  1:146579/141‑1 (MQ=255)
        tgatatgtgATTAACATACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                                                    <  2:448664/136‑1 (MQ=255)
        tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                                                    >  1:537626/1‑141 (MQ=255)
        tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                                                                                                                    <  1:549467/141‑1 (MQ=255)
               tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCa                                                                                                                             <  1:320163/141‑1 (MQ=255)
                 aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTATTTTCTAACATCTTAAAACCCAACCCCCCtt                                                                                                                           >  2:666771/1‑141 (MQ=255)
                  ttAACACACGTTGACCTGGTTTAATTGCATCTTTGTGAATTGTTAAAACATTTGTACTATATTCTAGGTCATACTCATAACGATTGACTTCACGAGGTACTTTCCCTTCTTTTCTATCAGGTGCAACGCCAATCCCCATTg                                                                                                                          >  1:275911/1‑141 (MQ=255)
                  ttAACACACCTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTg                                                                                                                          <  1:217061/141‑1 (MQ=255)
                    aaCACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                                                                                                                        <  1:497243/141‑1 (MQ=255)
                     acacacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCACCTTCTTTTCTAACAGGTGAAAAGCCACTCGCCATTGAAt                                                                                                                       >  2:182962/1‑141 (MQ=255)
                        cacGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCCAGTTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAg                                                                                                                    <  1:248362/141‑1 (MQ=255)
                         acGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGc                                                                                                                   >  1:443723/1‑141 (MQ=255)
                              gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACACAAAAAAACCTACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAg                                                                                                              >  1:355938/1‑141 (MQ=255)
                                ccTGGTTTAATTGTAGCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAATCTACAGGa                                                                                                            <  2:673641/141‑1 (MQ=255)
                                ccTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTACCCTTCTTTTCTAACAGCTGCAAAGCCAATGCCCATTGAATAAGCTACAgca                                                                                                            >  2:134300/1‑139 (MQ=255)
                                     tttAATTGCTTCTTTGTGTATTGGTAAAACATTTGTACCATAGTCTAGGTCATACTCATAACGAATGGCTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAACCAATCCCCATTGAATAAGCTACAGGACAgac                                                                                                       <  2:666377/141‑3 (MQ=255)
                                      ttAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCa                                                                                                      <  1:162320/141‑1 (MQ=255)
                                        aaTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTATGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAAt                                                                                                    <  2:597897/141‑1 (MQ=255)
                                         aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGTCTTCACTAGGTAATTTGCGCGATGATCGATCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATg                                                                                                   >  1:144218/1‑141 (MQ=255)
                                         aTTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATCAGCTACAGGACAGCCCATg                                                                                                   >  1:545678/1‑141 (MQ=255)
                                              aTCTTTGTGCATTGCTAACCCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGAtcat                                                                                              >  2:421497/1‑138 (MQ=255)
                                                 tttGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATCAGCTACAGGACAGCCAATGATAATGcc                                                                                           >  2:687827/1‑141 (MQ=255)
                                                  ttGTGCATTGTTAAAACATTTGTACCATATTCTATGGCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCa                                                                                          <  2:493211/141‑1 (MQ=255)
                                                   tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCATTCCCCATTTAATAAGCTACAGGAAAACCAATGATACGGCCAc                                                                                         >  1:627960/1‑141 (MQ=255)
                                                       cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                     <  1:61721/141‑1 (MQ=255)
                                                         ttGTTAAAACATTTGTACCATATTCTAGGTCATAATCATAACCAATGACTGCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCtt                                                                                   <  1:320812/141‑1 (MQ=255)
                                                               aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTc                                                                             <  2:227393/141‑1 (MQ=255)
                                                                aaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGATGCAAAGCCAATCCCCATTGAATAATCTACAGGACAGCCAATGATAAACCCACGCGCTTCAGGTcc                                                                            >  2:252241/1‑141 (MQ=255)
                                                                    tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCTACGCGCTTCAGGTCCTACa                                                                        >  1:420021/1‑141 (MQ=255)
                                                                    tttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACa                                                                        >  1:156048/1‑141 (MQ=255)
                                                                     ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACaa                                                                       >  1:266175/1‑141 (MQ=255)
                                                                         aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCATGTCCTGCAACgct                                                                   >  1:382174/1‑139 (MQ=255)
                                                                          ccATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGata                                                                  >  1:268035/1‑141 (MQ=255)
                                                                                  tAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTACCTTCTTTTCTAACAGTTGCAAAGCCAATCCCCATTAAATAAACTCCGAAGGAGCCACTGATAATACCAAGCGCTTCAGGTCCTACAACAATATCAACAtc                                                          >  2:189626/1‑141 (MQ=255)
                                                                                       cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCGTCAGGTCCTACAACGATATCAACATCTCTGt                                                     <  2:616547/141‑1 (MQ=255)
                                                                                       cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCAAATGATAATGCCACGCCCTACAGGTCCTACAACGATATCAACATCTATGt                                                     >  2:469634/1‑141 (MQ=255)
                                                                                        aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                                    <  2:570024/141‑1 (MQ=255)
                                                                                           cTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                 <  2:754334/141‑1 (MQ=255)
                                                                                           cTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCTACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                 <  2:394945/141‑1 (MQ=255)
                                                                                           cTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGCACAGGCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                 <  1:289975/141‑1 (MQ=255)
                                                                                           cTCATAACGAATGACTTCACGAGGGAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                 <  2:556265/141‑1 (MQ=255)
                                                                                                 aCGTATGACTTCACGAGGTAATTTCCCTTCTGTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTa                                           <  2:248906/141‑1 (MQ=255)
                                                                                                  cGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                                          <  1:5244/141‑1 (MQ=255)
                                                                                                      tGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGTTGCAAAGACAAACCCCGTTGAATCACACGCATGACATCCAATGATAAAGCCACGCGATTCAGGTCCTACACCGATATCAACATCTCTGTCTTTTCCGTATTCTa                                      >  1:178677/1‑141 (MQ=255)
                                                                                                           tCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtt                                 <  1:682751/141‑1 (MQ=255)
                                                                                                                   tcaTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACCATTTTATCTGt                         >  2:548952/3‑141 (MQ=255)
                                                                                                                       tcctcctcttTTCCAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCa                     <  1:666771/135‑1 (MQ=255)
                                                                                                                         cccTTCTTTTCTAACAGGTGCAAAGCCAATCCACATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATa                   <  1:559234/141‑1 (MQ=255)
                                                                                                                           cttcttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGc                 <  1:25109/141‑1 (MQ=255)
                                                                                                                             tcttTTCTAACAGGTGCAAATCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACTATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCa               <  2:393832/141‑1 (MQ=255)
                                                                                                                                tttCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAATGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCatat            >  1:509036/1‑141 (MQ=255)
                                                                                                                                     aaCAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGTATAGCCATATGCttt       >  1:451989/1‑140 (MQ=255)
                                                                                                                                        aGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc    <  1:530766/141‑1 (MQ=255)
                                                                                                                                        aGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATCCTTCAcc    >  1:432101/1‑141 (MQ=255)
                                                                                                                                        aGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTATCATCACCATATGCTTAAca    >  2:599669/1‑140 (MQ=255)
                                                                                                                                        aGGAGCAAACCCCATCACCGTTGATTAAGATAAAGGACAGCTAATAATAATGGCGCTCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGTGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc    <  2:198307/141‑1 (MQ=255)
                                                                                                                                          gTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACTCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt  <  2:266175/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAT  >  USA300TCH1516_ALE/1744308‑1744586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑