Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,428	T→C	K82E (AAA→GAA)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,428	0	T	C	100.0%	45.1 / NA	16	K82E (AAA→GAA)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/6);  total (10/6)

TAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTT  >  USA300TCH1516_ALE/1744305‑1744564                                                                                                                            |                                                                                                                                         tAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGACTAATTCCATCTTTGTCCATTATTAAAACATTTGTACCATATCCTAGGTCATCATTATACCGAATGACCTCACGAGCTAACTCCTCTTCTTTTCTacacg                                                                                                                         >  2:123044/1‑137 (MQ=255)      aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGCATCACTTCACCAGGTATTTCCCCTTCTTTTCTAACAgttcca                                                                                                                    >  1:129373/1‑136 (MQ=255)      aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCACCTTCTTTTCTAACAGGTGCa                                                                                                                    >  1:89841/1‑141 (MQ=255)               tgtGTAATTAACGCACGTTGACCTGGTTTAATTGCAGCTTGGTGCATTGTTAAAACAGTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATc                                                                                                           <  2:186663/139‑1 (MQ=255)                              cGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGAGAAGCCAATCCCCATTGAATAAGCt                                                                                            >  2:112935/1‑141 (MQ=255)                                   aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGTTCATACTCCTAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCAATAAAATAAGCTACacg                                                                                       >  2:121582/1‑139 (MQ=255)                                                       tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAc                                                                   >  1:209828/1‑141 (MQ=255)                                                         ttcATTGTTCAAACATTTCTACCACACTCTAGGTCATACTCCTAACGAATGCCTTCCCGAGGTAATTCCCCTCCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATACCCTACAGGACAGCCAATGATAAAGCCAcgc                                                                 <  1:169012/139‑1 (MQ=255)                                                                  aaaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGt                                                        >  1:207563/1‑141 (MQ=255)                                                                     aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                     >  1:235362/1‑141 (MQ=255)                                                                           gTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCTACAACg                                               >  1:101858/1‑141 (MQ=255)                                                                                                 cATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                         <  1:16571/141‑1 (MQ=255)                                                                                                 cATAACGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTg                         <  1:195913/141‑1 (MQ=255)                                                                                                  aTAACGAATGACTTCACGAGGTAATTCCCCTACTTTTCTAACAGGTGCAAAGCCAATCCCCCTTGCATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCACGTCCTACAACGATATCAACATCTCTGTCttttac                        >  2:244878/1‑139 (MQ=255)                                                                                                      cGAATGACTTCACGAGGTAATTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                    <  1:24800/141‑1 (MQ=255)                                                                                                                        aaTTCCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGtt  <  1:163225/141‑1 (MQ=255)                                                                                                                            |                                                                                                                                         TAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTT  >  USA300TCH1516_ALE/1744305‑1744564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑