Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F53 I1 R1 64 112.2 5657101 94.0% 5317674 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 119,744 (G)6→7 coding (464/855 nt) ampE → ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913119,7381.G97.8% 330.9 / 0.3 92coding (458/855 nt)ampEampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (40/50);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 106 total aligned reads displayed.

GGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑GGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGCATCATCG  >  NC_000913/119640‑119831
                                                                                                   |                                                                                               
ggCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATtgt‑g                                                                                                >  2:395023/1‑100 (MQ=255)
  cAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑ggg                                                                                              <  2:2759209/100‑1 (MQ=255)
  cAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑ggg                                                                                              <  2:1240766/100‑1 (MQ=255)
  cAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑ggg                                                                                              <  1:876957/100‑1 (MQ=255)
   aGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg                                                                                             <  2:2287282/100‑1 (MQ=255)
    ggCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g                                                                                           <  2:8539/100‑1 (MQ=255)
     gCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g‑g                                                                                         >  1:2732810/1‑100 (MQ=255)
       tGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGCTTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g‑gga                                                                                       >  2:2358063/1‑98 (MQ=255)
       tGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g‑gga                                                                                       >  2:158095/1‑98 (MQ=255)
       tGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g‑gg                                                                                        >  1:1114153/1‑98 (MQ=255)
         cgacgaACGTGAGTATTTGCGTGAGTTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g‑ggaac                                                                                     <  2:572616/100‑5 (MQ=255)
          nacgaACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑gggg‑g‑ggaacc                                                                                    >  2:1493058/2‑95 (MQ=255)
            cgaACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTg                                                                                   <  1:1085418/100‑1 (MQ=255)
            cgaACGTGAGTATTTGCGTGAGCTGCAAAATGCACTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCtn                                                                                   <  1:1493046/100‑2 (MQ=255)
             gaACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTgg                                                                                  <  2:2704245/100‑1 (MQ=255)
               aCGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTggg                                                                                 <  2:1277814/99‑1 (MQ=255)
                ngTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGGGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGa                                                                               >  2:1990394/2‑100 (MQ=255)
                cGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGa                                                                               >  2:2609075/1‑100 (MQ=255)
                 gTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGAc                                                                              <  1:25084/100‑1 (MQ=255)
                  tGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGAcc                                                                             >  1:1594054/1‑100 (MQ=255)
                  tGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGAcc                                                                             <  1:2463388/100‑1 (MQ=255)
                   gAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGAccc                                                                            >  1:488327/1‑100 (MQ=255)
                    aGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCg                                                                           <  1:2038626/100‑1 (MQ=255)
                    aGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCg                                                                           <  2:14904/100‑1 (MQ=255)
                      tATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGtt                                                                         <  2:277631/100‑1 (MQ=255)
                      tATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGtt                                                                         >  2:1755703/1‑100 (MQ=255)
                       aTTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTa                                                                        >  1:1870270/1‑100 (MQ=255)
                        tttGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTAc                                                                       <  1:996947/100‑1 (MQ=255)
                        tttGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTAc                                                                       >  1:472811/1‑100 (MQ=255)
                        tttGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAAACTGGGGACCCGTTAc                                                                       >  2:2776346/1‑100 (MQ=255)
                         ttGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACg                                                                      <  2:1274425/100‑1 (MQ=255)
                          tGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGc                                                                     >  2:2426602/1‑100 (MQ=255)
                           gCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCt                                                                    >  1:843668/1‑100 (MQ=255)
                            cGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTg                                                                   >  1:1250396/1‑100 (MQ=255)
                            cGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTg                                                                   <  1:286896/100‑1 (MQ=255)
                                aGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATgg                                                               >  2:641406/1‑100 (MQ=255)
                                  cTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATgggg                                                             <  2:1250404/100‑1 (MQ=255)
                                  cTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATgggg                                                             <  1:2175801/100‑1 (MQ=255)
                                  cTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATgggg                                                             <  1:2075502/100‑1 (MQ=255)
                                   tGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGt                                                            >  1:2487234/1‑100 (MQ=255)
                                   tGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGt                                                            >  1:1968953/1‑100 (MQ=255)
                                   tGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGt                                                            >  1:333409/1‑100 (MQ=255)
                                   tGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGt                                                            <  2:129663/100‑1 (MQ=255)
                                    gCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTa                                                           <  2:2627887/100‑1 (MQ=255)
                                    gCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTa                                                           <  1:2342322/100‑1 (MQ=255)
                                    gCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTa                                                           >  1:1584190/1‑100 (MQ=255)
                                    gCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTa                                                           >  2:679325/1‑100 (MQ=255)
                                    gCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTa                                                           >  2:2342341/1‑100 (MQ=255)
                                      aaaaTGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATg                                                         <  1:137018/100‑1 (MQ=255)
                                          tGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGtt                                                     <  2:1469331/100‑1 (MQ=255)
                                            cATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGtttt                                                   <  1:173149/100‑1 (MQ=255)
                                             ccgatcttgGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGtttt                                                   <  2:1815138/92‑1 (MQ=255)
                                              ttGCTGTGGATTAACTTTCGTTTGTATCTTGCACCGCTGTTCTGGCTGATTGT‑GGGGTG‑GAACCGGGGGACCCGTTACGCTGATGGGGTATGCGTGTTTg                                                 <  1:883213/100‑1 (MQ=255)
                                                 cTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGt                                              >  1:1625007/1‑100 (MQ=255)
                                                   gtgGATTCACTATCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGAACCGTTACGCTGATGGGGTATGCGTTTTTGCGTGc                                            >  1:1488575/1‑100 (MQ=255)
                                                   gtgGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGc                                            >  1:2534042/1‑100 (MQ=255)
                                                   gtgGATTAACTTTCGTTTGTATCTTGCCCCGCGGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGc                                            <  1:2608486/100‑1 (MQ=255)
                                                     ggATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCAt                                          >  2:323493/1‑100 (MQ=255)
                                                     ggATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCAt                                          <  1:1787723/100‑1 (MQ=255)
                                                      gATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTTATGGGGTATGCGTTTTTGCGTGCATg                                         <  2:476512/100‑1 (MQ=255)
                                                      gATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATg                                         <  2:535335/100‑1 (MQ=255)
                                                      gATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATg                                         <  2:550483/100‑1 (MQ=255)
                                                      gATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATg                                         <  2:703108/100‑1 (MQ=255)
                                                         tAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCa                                      >  2:828811/1‑100 (MQ=255)
                                                         tAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCa                                      <  2:2446924/100‑1 (MQ=255)
                                                           aCTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCact                                    >  2:2189106/1‑98 (MQ=255)
                                                           aCTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAAt                                    <  1:1295068/100‑1 (MQ=255)
                                                            cTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATa                                   >  1:1769710/1‑100 (MQ=255)
                                                             tttCGTGTTTATCTTGCACCGCGGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATAc                                  <  2:32856/100‑1 (MQ=255)
                                                              ttCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATAct                                 <  2:2732834/100‑1 (MQ=255)
                                                                cGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATActgg                               <  2:2507032/100‑1 (MQ=255)
                                                                cGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATActgg                               <  1:1031948/100‑1 (MQ=255)
                                                                 gTTTTTATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATActggc                              >  1:2435782/1‑100 (MQ=255)
                                                                  tttttATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATActggct                             <  2:2205955/100‑1 (MQ=255)
                                                                    tttATCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATActggctgg                           <  2:2193472/100‑1 (MQ=255)
                                                                       aTCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGgcgc                        >  2:490645/1‑100 (MQ=255)
                                                                        tCTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGcgcg                       <  2:1898510/100‑1 (MQ=255)
                                                                         cTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGa                      >  1:1360122/1‑100 (MQ=255)
                                                                         cTTGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGa                      >  2:1826434/1‑100 (MQ=255)
                                                                          ttGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGat                     <  1:519595/100‑1 (MQ=255)
                                                                           tGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGata                    >  1:1845212/1‑100 (MQ=255)
                                                                           tGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGata                    >  1:2751895/1‑100 (MQ=255)
                                                                           tGCACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGata                    <  1:95611/100‑1 (MQ=255)
                                                                             cACCGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATc                  <  1:2771732/100‑1 (MQ=255)
                                                                               ccGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAg                >  2:2387834/1‑100 (MQ=255)
                                                                               ccGCTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAg                >  2:1397270/1‑100 (MQ=255)
                                                                                  cTGTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGAcg             <  1:2363089/100‑1 (MQ=255)
                                                                                   tGTTCTGGCTGATTGT‑GGGG‑GTGAACCTGGGGACGCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCACTGCAATACTGGCTGGCGCGATATCAGAc              <  1:2189090/98‑1 (MQ=255)
                                                                                    gTTCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGAcgcc           >  1:1366030/1‑100 (MQ=255)
                                                                                      tCTGGCTGATTGTGGGGG‑G‑GAGCCTGGGGACCCGTTACGCTGGTGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGAcgccgc         <  1:1253289/100‑1 (MQ=255)
                                                                                      tCTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGAcgccgc         <  1:2387814/100‑1 (MQ=255)
                                                                                       gatcttGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGca        <  1:622422/95‑1 (MQ=255)
                                                                                       cTGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGca        >  2:738160/1‑100 (MQ=255)
                                                                                        tGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCTGACGCCGcan       <  1:1990379/100‑2 (MQ=255)
                                                                                        tGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGcat       >  1:699038/1‑100 (MQ=255)
                                                                                        tGGCTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGcat       <  2:626059/100‑1 (MQ=255)
                                                                                           cTGATTGTGGGGG‑G‑GGACATGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCCGACGCCGcatcat    >  1:878909/1‑100 (MQ=255)
                                                                                           cTGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGcatcat    >  1:2746811/1‑100 (MQ=255)
                                                                                            tGATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGcaagatc   >  2:622428/1‑95 (MQ=255)
                                                                                             gATTGTGGGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGCATCATCg  <  2:1893476/100‑1 (MQ=255)
                                                                                                   |                                                                                               
GGCAGGCTGCGACGAACGTGAGTATTTGCGTGAGCTGCAAAATGCATTGCTGTGGATTAACTTTCGTTTTTATCTTGCACCGCTGTTCTGGCTGATTGT‑GGGG‑G‑GAACCTGGGGACCCGTTACGCTGATGGGGTATGCGTTTTTGCGTGCATGGCAATACTGGCTGGCGCGATATCAGACGCCGCATCATCG  >  NC_000913/119640‑119831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A