Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,048,330 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,048,3300AT98.6% 500.8 / ‑2.7 211intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (112/95);  minor base C (2/1);  total (114/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,048,3310CG100.0% 498.9 / NA 211intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (114/97);  total (114/97)
*CP0425831,048,3320TA99.1% 502.7 / NA 211intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (112/97);  minor base C (2/0);  total (114/97)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

AACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGCTCAGGCGGGAACGCAAAAACGCGTACACACTCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGACTAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTG  >  CP042583/1048051‑1048611
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
          tgttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAt                                                                                                                                                                                                                                                                        <  2:837428/289‑1 (MQ=255)
                      aaaTCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATAACGCTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCcaca                                                                                                                                                                                                                                                            >  1:1092470/1‑289 (MQ=255)
                                cGGTAATGATTTGAATCGACAGGCGGGAACGCAAAGACGCGGACCCTGACTCCAGGTTGTTACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCTCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTGCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCCCC‑AGAATACCGTTTTCAATTTGTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCtt                                                                                                                                                                                                                                                   <  2:1599897/288‑1 (MQ=255)
                                cGGTAATGAGTTGAATCGACCGGCCGGAACCCAAAAACGCGTACACCTGCTCCAGGTTTGAACCTTCGTCATAATCATTGGGCGGGCTGCCGCTAAAGGTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCtt                                                                                                                                                                                                                                                   <  2:560861/288‑1 (MQ=255)
                                       gAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGa                                                                                                                                                                                                                                           <  1:404913/289‑1 (MQ=255)
                                          tggaatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACAt                                                                                                                                                                                                                                        >  2:468643/10‑289 (MQ=255)
                                             aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTc                                                                                                                                                                                                                                     >  1:1194345/7‑289 (MQ=255)
                                             aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTc                                                                                                                                                                                                                                     >  1:2065348/7‑289 (MQ=255)
                                               tcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCgg                                                                                                                                                                                                                                   >  2:1286783/5‑289 (MQ=255)
                                                  acAGGCGGGAACGCAAAAACGCGTACACTGACCCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAg                                                                                                                                                                                                                                                                                   <  2:326425/237‑1 (MQ=255)
                                                      gCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGTATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACCGGTCGGGGCttt                                                                                                                                                                                                                             >  1:2084439/1‑287 (MQ=255)
                                                          gAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCt                                                                                                                                                                                                                         <  1:468643/288‑1 (MQ=255)
                                                           aaCGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGccgaaggcacg                                                                                                                                                                                                                       >  2:624935/1‑280 (MQ=255)
                                                                  aaaCGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCa                                                                                                                                                                                                                <  1:1975203/289‑1 (MQ=255)
                                                                        gtacactgactcCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTg                                                                                                                                                                                                          >  1:299920/10‑289 (MQ=255)
                                                                             ctgactcCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAgg                                                                                                                                                                                                      <  1:2201267/285‑1 (MQ=255)
                                                                              tgactcCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAATGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGtt                                                                                                                                                                                                    >  1:871471/4‑289 (MQ=255)
                                                                                actcCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTc                                                                                                                                                                                                  >  1:327978/2‑289 (MQ=255)
                                                                                      ggTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCgg                                                                                                                                                                                                                                                <  1:1909270/237‑1 (MQ=255)
                                                                                      ggTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCg                                                                                                                                                                                            >  1:1130436/1‑289 (MQ=255)
                                                                                        ttGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCa                                                                                                                                                                                                 <  1:1545067/282‑1 (MQ=255)
                                                                                          gTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGa                                                                                                                                                                                                         <  1:894010/272‑1 (MQ=255)
                                                                                          gTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGa                                                                                                                                                                                                         >  2:894010/1‑272 (MQ=255)
                                                                                           tAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAAc                                                                                                                                                                                        >  1:1740844/1‑288 (MQ=255)
                                                                                              ccTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCCTTCAATGGTCAAGGTTTCATTCCGTACAgcca                                                                                                                                                                                    >  2:2014410/1‑289 (MQ=255)
                                                                                                tGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTg                                                                                                                                                                                                                                                                      <  1:968394/205‑1 (MQ=255)
                                                                                                tGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagc                                                                                                                                                                                  <  1:16095/289‑1 (MQ=255)
                                                                                                 gCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagc                                                                                                                                                                                  >  2:1917120/1‑288 (MQ=255)
                                                                                                  cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagcca                                                                                                                                                                                >  1:199677/1‑289 (MQ=255)
                                                                                                  cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGACCAGTGCAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGACGGGCCTTCCCCTTGCATTTCAATGGTGATGGTTTCATTCCGTAACg                                                                                                                                                                                       >  2:1545067/1‑282 (MQ=255)
                                                                                                       tAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCg                                                                                                                                                                                                                                                                              >  2:1840310/1‑190 (MQ=255)
                                                                                                         aTCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCa                                                                                                                                                                                                                <  2:1630893/250‑1 (MQ=255)
                                                                                                         aTCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCa                                                                                                                                                                                                                >  1:1630893/1‑250 (MQ=255)
                                                                                                            aTTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGTTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGt                                                                                                                                                                        >  1:8641/1‑287 (MQ=255)
                                                                                                             ttGTGCGTGTCGCCGCCAAATCCACCGTGAATAATCGCCTCCTGACGTGGCAAAATTCCCGCATTATCGCCCTGCAACGGCTTACGTTAGCACACGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTTTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCATCATCGCAAGATTGCCTCCCCACAACGGGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCt                                                                                                                                                                     <  2:1092470/289‑1 (MQ=255)
                                                                                                             ttGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCt                                                                                                                                                                     <  2:1194345/289‑1 (MQ=255)
                                                                                                             ttGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCt                                                                                                                                                                     <  1:1646501/289‑1 (MQ=255)
                                                                                                              tGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGCTTCCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATCGTGACGGTTTCATTGCGTAACGCCAGCCCGAACTGGTGCTc                                                                                                                                                                    >  2:1791648/1‑289 (MQ=255)
                                                                                                                  cGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGa                                                                                                                                                                                                         >  2:940203/1‑248 (MQ=255)
                                                                                                                  cGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTa                                                                                                                                                                >  1:1239535/1‑289 (MQ=255)
                                                                                                                    tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTg                                                                                                                                                                       >  1:1007919/1‑280 (MQ=255)
                                                                                                                     gctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCa                                                                                                                                                                                                 >  1:223947/1‑253 (MQ=255)
                                                                                                                     gctgaCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAg                                                                                                                                                             <  1:600908/289‑1 (MQ=255)
                                                                                                                      ctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCt                                                                                                                                                                                                                               <  1:1858857/222‑1 (MQ=255)
                                                                                                                      ctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTcc                                                                                                                                                                                                                            <  1:1427919/225‑1 (MQ=255)
                                                                                                                                    aCCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGCGCTGCAACATCATACGTTCGACCCGGAAAGGGTGCTCGTTAAAATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTTGCATCCACGGTGTACCGATCAGCGCAATCAGCATCGCAAGATAGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCAGGCCTTCCCCTTGCCCCTCAATGGTGGAGGTTTCAGTTCGTAACGTCTGCCTGAGATGGTGCTTGGTACCGCCgttcagttcccc                                                                                                                                               >  2:1361437/1‑285 (MQ=255)
                                                                                                                                          aataatCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCa                                                                                                                                        >  2:1035389/1‑289 (MQ=255)
                                                                                                                                               tCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGcc                                                                                                                                                                                                                                <  1:1277771/196‑1 (MQ=255)
                                                                                                                                               tCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCg                                                                                                                                                          <  2:1071816/266‑1 (MQ=255)
                                                                                                                                                    ttCTGACGTGGCAAACTTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCCCGGTAAAGGCGTTCAGTTCAGCCGCCCCAAAGTTTggt                                                                                                                               >  2:208939/1‑286 (MQ=255)
                                                                                                                                                    ttCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGcc                                                                                                                                                                                                                                <  2:1423645/191‑1 (MQ=255)
                                                                                                                                                    ttCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGcc                                                                                                                                                                                                                                >  1:1423645/1‑191 (MQ=255)
                                                                                                                                                    ttCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCt                                                                                                                               >  1:265009/1‑288 (MQ=255)
                                                                                                                                                    ttCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCt                                                                                                                               >  2:1442047/1‑288 (MQ=255)
                                                                                                                                                    ttCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTa                                                                                                                              <  2:1740844/289‑1 (MQ=255)
                                                                                                                                                     tCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAATGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTAc                                                                                                                             <  2:871471/289‑1 (MQ=255)
                                                                                                                                                             ggCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGATATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATgg                                                                                                                     >  2:279987/1‑289 (MQ=255)
                                                                                                                                                               cagaaTTCCCTCATGATCGAGATGCAACAGCATACGTTCGACCCGGAAAGGGAGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTGCTCAATTTTTGGCATCCACGGGGTACCGATCAGTGAAAGCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGAAATGTCGGGCCTTCCCATTGCCCTTCAATGGTGAAGGTTTCATGGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:670993/285‑1 (MQ=255)
                                                                                                                                                               cacaaTACCCGCATAATACTGCTGCCAAAGCATACGTTCGACCCGGAAAGGGGGCTCGTTAATATCTTCCCGCACC‑ATAATACCGTTCTCAATTTTTGGCATCCCCGGTGTACCGGTCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:1783116/285‑1 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGGTAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:2065348/288‑1 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:2035973/288‑1 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  1:2014410/288‑1 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:299920/288‑1 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:2205745/288‑1 (MQ=255)
                                                                                                                                                                     tCCCGCATGATAGGTCTGCGAACGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCGTCCAGCACC‑AGAATACCGTTCTCAATCTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCACCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTa                                                                                                             <  2:769416/289‑1 (MQ=255)
                                                                                                                                                                     tCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTa                                                                                                             <  1:1053872/289‑1 (MQ=255)
                                                                                                                                                                     tCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTa                                                                                                             <  1:1917120/289‑1 (MQ=255)
                                                                                                                                                                                    cTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTa                                                                                                                                                                >  1:153312/1‑223 (MQ=255)
                                                                                                                                                                                    cTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACAtt                                                                                               >  1:2146476/1‑288 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCACTGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTATAGGCGTTCAGTTCATCCGCCCCAAAGTTTGCTACCAGCATCGGCCCGCGAAAGGTGATGATATTGcc                                                                                            >  2:1482073/1‑288 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGt                                                                                                                                    <  2:1242349/248‑1 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGt                                                                                                                                    >  1:1242349/1‑248 (MQ=255)
                                                                                                                                                                                        aaCCGCATACTGTCGACCCTGAAAGGTTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCAACCACGGTGTACCGATCAGGGAAATCAGCATCGCAAGATGGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCa                                                                                           <  2:8641/288‑1 (MQ=255)
                                                                                                                                                                                             cATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGTCTTCATTGCGTAACGCCAGCCAGAAATGGTGTTCGGTACAGGCGTTCAGTCCATCCGCGCCAGAGTTTGCTACCAGCATGGGGCAGCTCAAGGTGATGACATTGACAGgcgc                                                                                      >  2:1874603/1‑288 (MQ=255)
                                                                                                                                                                                                   ttCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCagaaga                                                                               <  2:2146476/289‑1 (MQ=255)
                                                                                                                                                                                                       aCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATc                                                                                                                                               <  1:1213657/221‑1 (MQ=255)
                                                                                                                                                                                                       aCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATc                                                                                                                                               >  2:1213657/1‑221 (MQ=255)
                                                                                                                                                                                                       aCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGc                                                                           >  1:704959/1‑289 (MQ=255)
                                                                                                                                                                                                        cccGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATAGCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCa                                                                          <  1:522356/289‑1 (MQ=255)
                                                                                                                                                                                                             aaaGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTg                                                                                                                                                                       <  1:293806/191‑1 (MQ=255)
                                                                                                                                                                                                              aaGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAAt                                                                    <  1:1791648/289‑1 (MQ=255)
                                                                                                                                                                                                                  gTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGtga                                                                                                      <  2:342073/251‑1 (MQ=255)
                                                                                                                                                                                                                   tGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTCCAGTTCATCCGCACCAAAGTTTGCTACCAGCATGGGGCCGCTAACGGTGATGACATTGCCATGCGGCACAAGCCCGccatcgatggcga                                                               >  2:1078791/1‑277 (MQ=255)
                                                                                                                                                                                                                     cTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGTCAAAGGTGATGACATTGCCCTTCGCCAGAAGACCGGACTGCATGGCGGTa                                                             >  2:808188/1‑289 (MQ=255)
                                                                                                                                                                                                                     cTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTa                                                             >  2:1806109/1‑289 (MQ=255)
                                                                                                                                                                                                                      tCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTaa                                                            >  1:1824512/1‑289 (MQ=255)
                                                                                                                                                                                                                       cGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGCTGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTaaa                                                           >  1:2217263/1‑289 (MQ=255)
                                                                                                                                                                                                                             tatCTTCCATCACC‑AGCATACCGTTCTCAATTTTTGGCATCCACGGGTTACCGATCAGTGAAATCAGCATCGCACGATGGCCTCCCCACAACGTGCCGTCGGCCCGACATGTCGGGCCTTCCCCGTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCt                                                     <  2:2256493/289‑1 (MQ=255)
                                                                                                                                                                                                                             tatCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCt                                                     <  2:704959/289‑1 (MQ=255)
                                                                                                                                                                                                                                  tCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAAt                                                                    >  2:656343/1‑269 (MQ=255)
                                                                                                                                                                                                                                    cAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTg                                                                                                                                                                       <  2:1225261/168‑1 (MQ=255)
                                                                                                                                                                                                                                    cAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTg                                                                                                                                                                       >  1:1225261/1‑168 (MQ=255)
                                                                                                                                                                                                                                         cc‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGt                                                                                                        <  1:833130/226‑1 (MQ=255)
                                                                                                                                                                                                                                          c‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATg                                        <  1:1490390/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCCACGTTTGCTACCAGAATGGGGCCGCTAAAGGTGATGACATTGCCACGCGCCAGAAGACCGAACTGAATGGCGGTAAAATCGCTAATCCCGCAATAGATCCACggg                               >  2:771464/1‑288 (MQ=255)
                                                                                                                                                                                                                                                     ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACggg                               >  1:1517332/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    aagggacaggtACCGATCAGTGAAATCAGCATAGCATGCTTGCCTCCCCACCACGGGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAg               <  2:1013787/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aTCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCGATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGa                >  1:772617/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                ccGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGa                                                                                                                                                                                                         >  2:535184/1‑91 (MQ=255)
                                                                                                                                                                                                                                                                                ccGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGa                                                                                                                                                                                                         <  1:535184/91‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
AACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGCTCAGGCGGGAACGCAAAAACGCGTACACACTCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACC‑AGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGACTAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTG  >  CP042583/1048051‑1048611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A