Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,048,129 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,048,1290AT99.7% 924.8 / NA 388intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (201/184);  total (202/186)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,048,1300CG99.5% 922.4 / NA 388intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (200/185);  minor base T (2/0);  total (202/186)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,048,1310TA99.5% 929.8 / NA 388intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (202/184);  minor base C (0/2);  total (202/186)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

GTTGTGGCATCCAACGGTCGGTTACTCCTGATTACGCTGTCTCTTATATAAACCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGCTCAGGCGGGAACGCAAAAACGCGTACACACTCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGACTAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCA  >  CP042583/1047853‑1048405
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                  
                   ggtTACTCCTGATTACGCTGTCTCTTATATAAACCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAAAGCGTACACTGACTCCAGGGTGTAAACTGCGTCAAAAtaaa                                                                                                                                                                                                                                                       >  2:1250016/1‑286 (MQ=255)
                         tCCTGATTACGCTGTCTCTTATATAAACCCTACCATATTAGCGGCAGAGACCGCGTTTTACTTAGCCCTGAAGCGTGATTTGTTTACATTTTCAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGATCGCAAAAACGCGTACACTGACTCCAGGt                                                                                                                                                                                                                                                                            <  1:1562012/262‑1 (MQ=255)
                                       tctcTTATATAAACCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGGACAGGAGGGAACGCAAAAACGCGTACACTGACTTCAGGTTGTAACCTGCGTCAGAATAATTGGGCGTGCTGCCGCTcaa                                                                                                                                                                                                                                    >  1:2408967/1‑288 (MQ=255)
                                       tctcTTATATAAACCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTaaa                                                                                                                                                                                                                                    >  1:840410/1‑288 (MQ=255)
                                        ctctTATATAAACCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATCTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACCCCAGGTT‑TAACCTGCGTCAAAATCATTGGGCGTGATGCCGCTAAAGCt                                                                                                                                                                                                                                 >  2:1276939/1‑289 (MQ=255)
                                                 aaaCCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGaa                                                                                                                                                                                                                         >  1:256033/1‑289 (MQ=255)
                                                   aCCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTTAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGCAATCGACAGGCTGGAACGCAAACACGCGTACCCTGACTCCAGGTTGTACCCTGCGTCCTACTCATTGAGCGTGCTTCGTATGAAGCTACCGAGaaaa                                                                                                                                                                                                                       >  2:2745893/1‑287 (MQ=255)
                                                   aCCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCa                                                                                                                                                                                                                                                              >  1:2098585/1‑250 (MQ=255)
                                                   aCCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGaata                                                                                                                                                                                                                       >  1:2436546/1‑289 (MQ=255)
                                                        aCCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTg                                                                                                                                                                                                                                                                   >  2:872056/1‑240 (MQ=255)
                                                               tAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCCACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGGGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTTGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTg                                                                                                                                                                                                            >  1:3310199/1‑288 (MQ=255)
                                                               tAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTTACTCCAGGTTGTAACCTTCGTGATAATCATTGGTGGTGCTGCAGCTAAAGCTACCGAGGATAATCGACTTCTGa                                                                                                                                                                                                           >  1:2623395/1‑289 (MQ=255)
                                                               tAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTg                                                                                                                                                                                                            >  1:1904782/1‑288 (MQ=255)
                                                                aGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGAc                                                                                                                                                                                                          <  1:91263/289‑1 (MQ=255)
                                                                 gCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCa                                                                                                                                                                                                                                                              >  1:2865703/1‑236 (MQ=255)
                                                                   ggCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTg                                                                                                                                                                                                       >  1:1142300/1‑289 (MQ=255)
                                                                   ggCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTg                                                                                                                                                                                                       >  2:1711581/1‑289 (MQ=255)
                                                                    gCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTgg                                                                                                                                                                                                      >  2:219751/1‑289 (MQ=255)
                                                                            aGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAAtt                                                                                                                                                                                               >  2:2551402/1‑288 (MQ=255)
                                                                               gTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTc                                                                                                                                                                                              <  1:2551402/286‑1 (MQ=255)
                                                                               gTTTTACTTAGCCCTGAAGCGTGATTTTTTTACAGTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTc                                                                                                                                                                                              <  2:819219/286‑1 (MQ=255)
                                                                                ttttACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCg                                                                                                                                                                                           <  1:1648066/288‑1 (MQ=255)
                                                                                ttttACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCg                                                                                                                                                                                           <  1:3062550/288‑1 (MQ=255)
                                                                                     cTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGAt                                                                                                                                                                                     <  2:1358159/289‑1 (MQ=255)
                                                                                        aGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATaga                                                                                                                                                                                  >  1:67381/1‑289 (MQ=255)
                                                                                             tGAACCGTGATTTTTTTCCATTTTAAGACCAGGATGACCGCTAATCGTCAACTGGGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGc                                                                                                                                                                             <  2:3093478/289‑1 (MQ=255)
                                                                                                    tGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTTGCAAAATTCCCGCATGATAGGGCCGCAACCGCa                                                                                                                                                                      >  2:3286079/1‑289 (MQ=255)
                                                                                                    tGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCa                                                                                                                                                                      >  2:1174052/1‑289 (MQ=255)
                                                                                                    tGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGAAAGAGCTGCAACAgac                                                                                                                                                                      >  2:44242/1‑287 (MQ=255)
                                                                                                     gATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCa                                                                                                                                                                      <  2:67381/288‑1 (MQ=255)
                                                                                                            ttACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCg                                                                                                                                                              >  1:2666966/1‑289 (MQ=255)
                                                                                                               cATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGAcc                                                                                                                                                           <  2:156573/289‑1 (MQ=255)
                                                                                                               cATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGAcc                                                                                                                                                           <  1:1838764/289‑1 (MQ=255)
                                                                                                                              aTGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCt                                                                                                                                              <  2:1871086/287‑1 (MQ=255)
                                                                                                                              aTGACCGCGAATCGTCACCTGGGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTg                                                                                                                                                <  2:2839127/285‑1 (MQ=255)
                                                                                                                                 aCCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACg                                                                                                                                                                                                         >  2:2229980/1‑225 (MQ=255)
                                                                                                                                  ccGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCt                                                                                                                                                                                                                                                                    <  2:3021100/165‑1 (MQ=255)
                                                                                                                                             cAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTGATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                                              <  1:274254/288‑1 (MQ=255)
                                                                                                                                             cAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                                              <  1:3159353/288‑1 (MQ=255)
                                                                                                                                             cAACTGAGTGCCTTCCCGGGGGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                                              <  2:2150733/288‑1 (MQ=255)
                                                                                                                                                 tGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCAc                                                                                                                           <  1:94837/287‑1 (MQ=255)
                                                                                                                                                  gAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCg                                                                                                                                                                                           <  1:3217843/222‑1 (MQ=255)
                                                                                                                                                   aGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAg                                                                                                                                                                                                                                   <  1:2901034/181‑1 (MQ=255)
                                                                                                                                                   aGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAACATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTAGt                                                                                                                                           >  1:3020271/1‑269 (MQ=255)
                                                                                                                                                   aGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGa                                                                                                                       >  1:1179227/1‑289 (MQ=255)
                                                                                                                                                   aGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCCACCCCGAAAGGGGTCCCGGTAATATCTTCCAGCACCAgc                                                                                                                       >  2:1440531/1‑288 (MQ=255)
                                                                                                                                                        cTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGa                                                                                                                                                                                      <  1:2587636/221‑1 (MQ=255)
                                                                                                                                                          tCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGt                                                                                                                                                                                                                                                                <  1:79519/145‑1 (MQ=255)
                                                                                                                                                                    tATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAAGCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAAttt                                                                                                       <  1:2942967/288‑1 (MQ=255)
                                                                                                                                                                      ttCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGTTAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTg                                                                                                                                                <  1:702726/245‑1 (MQ=255)
                                                                                                                                                                      ttCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCaa                                                                                                                                                                           <  2:3246472/218‑1 (MQ=255)
                                                                                                                                                                      ttCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTg                                                                                                                                                >  2:702726/1‑245 (MQ=255)
                                                                                                                                                                        aaGAATGGCATGGGCGACCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGc                                                                                                  <  2:2666966/288‑1 (MQ=255)
                                                                                                                                                                           aaTGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCt                                                                                                                                                                                                                                                                    >  1:1518480/1‑124 (MQ=255)
                                                                                                                                                                           aaTGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATTGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTTCTCGTTAATATCTTCCAGCCCCAAAATACCGGTACCAAGTTTTGGCAt                                                                                                >  2:423575/1‑288 (MQ=255)
                                                                                                                                                                            aTGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                                                <  1:162010/287‑1 (MQ=255)
                                                                                                                                                                             tGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGt                                                                                                                                                                                                                                                                >  2:1757440/1‑126 (MQ=255)
                                                                                                                                                                             tGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  1:2243114/289‑1 (MQ=255)
                                                                                                                                                                             tGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  1:44242/289‑1 (MQ=255)
                                                                                                                                                                             tGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  1:1269146/289‑1 (MQ=255)
                                                                                                                                                                             tGGCATGGGGGGCCAACGGCAGCGTAACCGTGCGGTGTTCATGACCAAAATCGAGGTCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  2:2877601/289‑1 (MQ=255)
                                                                                                                                                                             tGGAATGTCCGCCCAACGGCAGCGCAACCGTGTGTTCGTCAGAGCCAAATTCGTAGGCGGTAATGAGTTGAAACGACAGGCGGTAAGGCAAAAACGCGTACCCTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGAAAAATTCCCGCATGATATAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  2:2408967/289‑1 (MQ=255)
                                                                                                                                                                                cATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAata                                                                                                                                      >  1:2798275/1‑245 (MQ=255)
                                                                                                                                                                                     gcgcCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATa                                                                                                                                                                                                                                                            <  2:296944/122‑1 (MQ=255)
                                                                                                                                                                                     gcgcCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACg                                                                                                                                                                                                         <  2:1625659/173‑1 (MQ=255)
                                                                                                                                                                                     gcgcCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAACGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCCCAATTTTTGGCATCCACGGTGTa                                                                                      >  2:567504/1‑288 (MQ=255)
                                                                                                                                                                                     gcgcCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTa                                                                                      >  2:2002442/1‑288 (MQ=255)
                                                                                                                                                                                                cAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                                                >  2:2439794/1‑267 (MQ=255)
                                                                                                                                                                                                 agacgaaCCGTGCGTGGTGCATAACCAAAATCGGAGCAGTTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGAGTCCAGGTTGTAACCTGCGTCAGAATCATTGGGCGTGCAGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGGGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGa                                                                                  <  2:504230/275‑1 (MQ=255)
                                                                                                                                                                                                   cGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATag                                                                                                                                                                                   <  2:2803412/181‑1 (MQ=255)
                                                                                                                                                                                                   cGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTg                                                                                                                                                <  2:2569219/216‑1 (MQ=255)
                                                                                                                                                                                                   cGTAACCGTGCGGTGTTCATAACCAAAATCGGGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTtctc                                                                                                            <  2:342076/252‑1 (MQ=255)
                                                                                                                                                                                                           tGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAgtgaaatcctgtct                                                                 >  1:504230/1‑274 (MQ=255)
                                                                                                                                                                                                             cGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  2:3170237/257‑1 (MQ=255)
                                                                                                                                                                                                             cGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAgt                                                                             <  2:1823839/273‑2 (MQ=255)
                                                                                                                                                                                                                tgttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATTGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAt                                                          <  2:2926644/289‑1 (MQ=255)
                                                                                                                                                                                                                tgttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAt                                                          <  1:1431005/289‑1 (MQ=255)
                                                                                                                                                                                                                 gttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAtt                                                         >  1:2166404/1‑289 (MQ=255)
                                                                                                                                                                                                                  ttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGtgct                                                                                                                                                                                                                                             <  2:230206/108‑1 (MQ=255)
                                                                                                                                                                                                                  ttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAtt                                                         >  1:1054734/1‑288 (MQ=255)
                                                                                                                                                                                                                   tCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGc                                                       <  1:585725/289‑1 (MQ=255)
                                                                                                                                                                                                                    cATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGcc                                                      <  2:1054734/289‑1 (MQ=255)
                                                                                                                                                                                                                     aTGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGCCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGAt                                                                                                                                                                                     <  2:2096336/161‑1 (MQ=255)
                                                                                                                                                                                                                      tGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATc                                                                 >  1:199696/1‑276 (MQ=255)
                                                                                                                                                                                                                         ccAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTccc                                                  <  1:1358036/288‑1 (MQ=255)
                                                                                                                                                                                                                            aaaTCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                                              <  2:109944/209‑1 (MQ=255)
                                                                                                                                                                                                                            aaaTCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCcaca                                              >  2:1508714/1‑289 (MQ=255)
                                                                                                                                                                                                                               ctgAGGCCGGTAATTGTTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGCATCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACg                                           <  2:2621334/287‑1 (MQ=255)
                                                                                                                                                                                                                                              aGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCACGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCAt                                                                                                >  1:128110/1‑221 (MQ=255)
                                                                                                                                                                                                                                                   aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATag                                                                                                                                                                                   <  2:2498863/127‑1 (MQ=255)
                                                                                                                                                                                                                                                   aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATag                                                                                                                                                                                   >  1:2498863/7‑133 (MQ=255)
                                                                                                                                                                                                                                                   aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTcc                                                                                                                                <  1:1727748/178‑1 (MQ=255)
                                                                                                                                                                                                                                                   aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             <  1:2210394/213‑1 (MQ=255)
                                                                                                                                                                                                                                                     tcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGa                             <  1:3256829/277‑1 (MQ=255)
                                                                                                                                                                                                                                                       gacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTGGTAACCTGCGTCATAATCATTTGGCGTGCGGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATGCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCAGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGc                   <  1:1778246/287‑1 (MQ=255)
                                                                                                                                                                                                                                                        acAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             >  1:2020487/2‑214 (MQ=255)
                                                                                                                                                                                                                                                        acAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGc                                                       >  1:388168/2‑252 (MQ=255)
                                                                                                                                                                                                                                                          aGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTcc                                                                                                                                >  1:1069299/1‑177 (MQ=255)
                                                                                                                                                                                                                                                               ggAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAg                                                                                                                              >  1:2827130/1‑174 (MQ=255)
                                                                                                                                                                                                                                                                   cGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCa                                                                                             >  2:936902/1‑203 (MQ=255)
                                                                                                                                                                                                                                                                                    |||                                                                                                                                                                                                                                                                                  
GTTGTGGCATCCAACGGTCGGTTACTCCTGATTACGCTGTCTCTTATATAAACCCTACCATATTAGCGGCAGAGACAGCGTTTTACTTAGCCCTGAAGCGTGATTTTTTTACATTTTAAGAACAGGATGACCGCTAATCGTCAACTGAGTGCCTTCCCGGGTGTTATTCAGAATGGCATGTGCGCCCAACGGCAGCGTAACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGCTCAGGCGGGAACGCAAAAACGCGTACACACTCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGACTAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCA  >  CP042583/1047853‑1048405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A