Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,048,330 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,048,3300AT97.9% 453.5 / NA 195intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (98/92);  minor base G (1/3);  total (100/95)
Fisher's exact test for biased strand distribution p-value = 3.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,048,3310CG99.5% 457.2 / NA 195intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (99/94);  minor base A (0/1);  total (100/95)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,048,3320TA99.5% 455.1 / ‑5.2 195intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (99/94);  minor base G (1/0);  total (100/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 195 total aligned reads displayed.

AACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGCTCAGGCGGGAACGCAAAAACGCGTACACACTCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGACTAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTG  >  CP042583/1048051‑1048611
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
    gTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCg                                                                                                                                                                                                                                                                              <  1:728897/289‑1 (MQ=255)
     tGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGc                                                                                                                                                                                                                                                                             >  1:1618826/1‑289 (MQ=255)
     tGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGAAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATAACTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATACACGGTGTACAGATCAGGTAAATCAGCATCga                                                                                                                                                                                                                                                                             >  1:1066765/1‑288 (MQ=255)
          tgttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAt                                                                                                                                                                                                                                                                        <  1:448557/289‑1 (MQ=255)
            ttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGGAATCAGCATCGCAAGAtt                                                                                                                                                                                                                                                                       >  1:170473/1‑288 (MQ=255)
            ttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAtt                                                                                                                                                                                                                                                                       >  1:599874/1‑288 (MQ=255)
            ttCATGACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGAtt                                                                                                                                                                                                                                                                       >  1:33669/1‑288 (MQ=255)
              cATGAACAAAATCGGGGCCGGTAATGAGTGGAAGCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGcc                                                                                                                                                                                                                                                                    <  2:1234648/289‑1 (MQ=255)
                 gACCAAAATCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAAAACCGTTTCCAAATTTTGGCATCCACGGTGTACCGGTCAGTGAAATCAGCATCGCAAGATTGGCtt                                                                                                                                                                                                                                                                  >  2:652238/1‑287 (MQ=255)
                      aaaTCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTTCAGGTTGTAACCTGCGTCATAATAATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGATCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGAATCCACGGTGTACCGACCAGCGAAATCAGCCTCGCAAGCTGGCCTCCCcaca                                                                                                                                                                                                                                                            >  2:1017992/1‑289 (MQ=255)
                      aaaTCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCcaca                                                                                                                                                                                                                                                            >  2:1097907/1‑289 (MQ=255)
                       aaTCGAGGCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCcaca                                                                                                                                                                                                                                                            >  2:375057/1‑288 (MQ=255)
                             ggCCGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTg                                                                                                                                                                                                                                                       >  1:273473/1‑287 (MQ=255)
                               ccGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCt                                                                                                                                                                                                                                                    <  2:1389692/288‑1 (MQ=255)
                               ccGGTAATGAGTGGAATCGACAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCt                                                                                                                                                                                                                                                    <  1:912554/288‑1 (MQ=255)
                                            gaatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATg                                                                                                                                                                                                                                       >  1:8413/8‑288 (MQ=255)
                                             aatcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGt                                                                                                                                                                                                                                      <  1:579344/282‑1 (MQ=255)
                                              atcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTc                                                                                                                                                                                                                                     <  1:1836288/283‑1 (MQ=255)
                                              atcgacAGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTc                                                                                                                                                                                                                                     <  1:653001/283‑1 (MQ=255)
                                                    aGGCGGGAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCt                                                                                                                                                                                                                               >  2:1043166/1‑288 (MQ=255)
                                                       cGGGAACGCAAAAACGCGTGCACTGACTCCAGGTAGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGccg                                                                                                                                                                                                                               <  2:227613/285‑2 (MQ=255)
                                                         ggAACGCAAAAACGCGTACACTGACTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCt                                                                                                                                                                                                                         >  1:1299351/1‑289 (MQ=255)
                                                                                actcCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGCCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGttta                                                                                                                                                                                                  >  2:166158/2‑288 (MQ=255)
                                                                                 ctcCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCa                                                                                                                                                                                                 <  2:599874/289‑1 (MQ=255)
                                                                                      ggTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCg                                                                                                                                                                                            >  1:1692560/1‑289 (MQ=255)
                                                                                          gTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCaa                                                                                                                                                                                                               >  1:36071/1‑266 (MQ=255)
                                                                                          gTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAAc                                                                                                                                                                                        >  1:189164/1‑289 (MQ=255)
                                                                                           tAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACg                                                                                                                                                                                       <  2:522543/289‑1 (MQ=255)
                                                                                            aaCCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCATTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATTCCGGGACTTCCCCTTGCCATTCATTGGGAGAGGTTTCATTggtcaacc                                                                                                                                                                                       >  2:1862729/1‑281 (MQ=255)
                                                                                                 gCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagcc                                                                                                                                                                                 >  1:1865747/1‑289 (MQ=255)
                                                                                                 gCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagcc                                                                                                                                                                                 >  1:953091/1‑289 (MQ=255)
                                                                                                  cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACTTGCCTTCGGCCCGAAATCTCTGGCCTTCCCCTTGCCATTCACTGGTCCAGGTTTCATTGCGTAAAgcaagcc                                                                                                                                                                                 >  2:441537/1‑288 (MQ=255)
                                                                                                  cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagcca                                                                                                                                                                                >  1:1907847/1‑289 (MQ=255)
                                                                                                  cGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACgccagcc                                                                                                                                                                                 >  2:1245165/1‑288 (MQ=255)
                                                                                                           cATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTg                                                                                                                                                                       <  1:1245165/289‑1 (MQ=255)
                                                                                                           cATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTg                                                                                                                                                                       <  2:112014/289‑1 (MQ=255)
                                                                                                                  cGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGCTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTa                                                                                                                                                                >  1:791855/1‑289 (MQ=255)
                                                                                                                    tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTaaa                                                                                                                                                              >  2:84571/1‑289 (MQ=255)
                                                                                                                    tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTaaa                                                                                                                                                              >  1:1779021/1‑289 (MQ=255)
                                                                                                                    tgctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTaa                                                                                                                                                               <  2:1618826/288‑1 (MQ=255)
                                                                                                                     gctgcCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTaaa                                                                                                                                                              <  1:1475286/288‑1 (MQ=255)
                                                                                                                        gcCGCTAAAGCTCCCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCg                                                                                                                                                          <  2:962523/289‑1 (MQ=255)
                                                                                                                                   tACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACg                                                                                                                                                                                       <  1:650770/249‑1 (MQ=255)
                                                                                                                                          aataatCGCGTTCTTACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATGTTTGGCATCCACGGTGTACCGATCTGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCa                                                                                                                                        <  1:1665381/289‑1 (MQ=255)
                                                                                                                                          aataatCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGATCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCa                                                                                                                                        >  2:149435/1‑289 (MQ=255)
                                                                                                                                                 cccTTCTGACGGGGCAAAATTCCCGCATGGTAGAGCTGCAACAGCATACGTTCGCCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACACCGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTg                                                                                                                                 <  2:700739/288‑1 (MQ=255)
                                                                                                                                                     tttGACGTGGCAACATTACCGCATGATAGAGCTGCAACAGAATACGTTCGACCCGGAAAGGGTGCTCTTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCCCGGTGTACCGATCAGTGAAATCAGCATCGCACGATTGCCTCCCCACAAAGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTAc                                                                                                                             <  1:1315895/287‑1 (MQ=255)
                                                                                                                                                     tcagcggTGGCAAAATTCCCGGATGAATGAGCTGAAACAGCATACGATCGACCCGGAAATGGTGATCGTTAATATCTTCCAGCACCAGAATACCGTTGTCAATTTTTGGCATCCACGGTGTAACGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCAGACATGTCGGGCCTTCCCCATGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTAc                                                                                                                             <  2:1788348/283‑1 (MQ=255)
                                                                                                                                                     tCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTAc                                                                                                                             <  1:1511754/289‑1 (MQ=255)
                                                                                                                                                     tCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAACATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTAc                                                                                                                             <  1:533223/289‑1 (MQ=255)
                                                                                                                                                      cTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCt                                                                                                                                                                                                                                                    >  2:360472/1‑169 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:678191/288‑1 (MQ=255)
                                                                                                                                                               cAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  1:199351/288‑1 (MQ=255)
                                                                                                                                                               cAAAAGTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTACCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  1:1225592/288‑1 (MQ=255)
                                                                                                                                                               aaaaaTTCCCGCATGTTAGGGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATggg                                                                                                                    <  2:1333749/287‑1 (MQ=255)
                                                                                                                                                                aaaaTTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATgggg                                                                                                                   <  1:1369258/288‑1 (MQ=255)
                                                                                                                                                                        cGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCg                                                                                                                                                                                                                                            >  2:1518973/1‑159 (MQ=255)
                                                                                                                                                                        cGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCg                                                                                                                                                                                                                                            <  1:1518973/159‑1 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTTCTACCAGCCTGGGGCCCCTAAAGTGGATGCCATTGccc                                                                                           >  2:1267202/1‑288 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCa                                                                                           >  1:1087493/1‑289 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCa                                                                                           >  1:188399/1‑289 (MQ=255)
                                                                                                                                                                                       cAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCa                                                                                           >  1:1189279/1‑289 (MQ=255)
                                                                                                                                                                                        aaCAGCATACGTTCGACCCGGGAAGGGTGCTCGTTAATATCTTCCAGGACCAGAATACCGTTCTCAAGTTTTGGCATCCACGGTGGACCGATCAGTGAACTCAGCATCGCAAGATTGCCTCCCCACGACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCa                                                                                           <  2:538673/288‑1 (MQ=255)
                                                                                                                                                                                        aaCAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCa                                                                                           <  2:646806/288‑1 (MQ=255)
                                                                                                                                                                                         aCAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCAt                                                                                          >  2:734094/1‑288 (MQ=255)
                                                                                                                                                                                         aCAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATg                                                                                         <  2:1195290/289‑1 (MQ=255)
                                                                                                                                                                                         aCAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATg                                                                                         <  1:1639870/289‑1 (MQ=255)
                                                                                                                                                                                         aCAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATg                                                                                         <  2:953091/289‑1 (MQ=255)
                                                                                                                                                                                             cATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGc                                                                                                                                                                                                                                 <  1:1147557/149‑1 (MQ=255)
                                                                                                                                                                                               tACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATAACGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCAGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCAGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTCACGGTGATGACATTTCCagtcaccag                                                                                   >  2:1284867/1‑281 (MQ=255)
                                                                                                                                                                                                    tCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAg                                                                                                                                                             >  1:707611/1‑210 (MQ=255)
                                                                                                                                                                                                       aCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCCGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCCACCAGCCTGGGGCCGCTCAAGGGGATGACTTTGCCAGTCGCCAGAAGCCCGc                                                                           >  2:1473201/1‑289 (MQ=255)
                                                                                                                                                                                                       aCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCg                                                                            >  1:312262/1‑288 (MQ=255)
                                                                                                                                                                                                       aCCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGc                                                                           >  1:604261/1‑289 (MQ=255)
                                                                                                                                                                                                                     cTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTa                                                             >  1:1578952/1‑289 (MQ=255)
                                                                                                                                                                                                                     cTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTa                                                             >  1:1008686/1‑289 (MQ=255)
                                                                                                                                                                                                                     cTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGGGAAGGTTTCATTGCGTAACGCCAGCCAGACATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGGTACCAGCATGGGGGCGCTAAAGGGGATGACATTTCCATGCGCCAGACAACCGAACTGAATGGCGCTa                                                             >  2:1759465/1‑289 (MQ=255)
                                                                                                                                                                                                                       cGTTAATTACTTCCACCACCATAAACCCGTGCTCAATTTTTGGACGCCACGGTGTCCCGATCAGGAAAATCCGCATCGCAAGATTGCCTCCCCCCACCGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTaaa                                                           <  2:1066765/289‑1 (MQ=255)
                                                                                                                                                                                                                               ccTTGCAGCACCAGACTACCTTTCCTCATTTTTGGCATCCACGGTGGAACGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTAt                                                   <  2:1079093/288‑1 (MQ=255)
                                                                                                                                                                                                                                  tCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCCCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATTGCCCAGAAGACCGCCCCGAATGGCGGGAAAATCCCTATGt                                                 >  2:22010/1‑288 (MQ=255)
                                                                                                                                                                                                                                        aCCAAAATACCGTACTCAATTTTTTGCAACCACGGTGTACCGATCAGTGCAATCAGCATCGCACGATGGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCaaa                                          <  2:170473/289‑1 (MQ=255)
                                                                                                                                                                                                                                         cccgAATACCGTTCTCAATTTTTGGCATCCGCGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAAt                                         <  1:1017992/286‑1 (MQ=255)
                                                                                                                                                                                                                                         ccAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTcccc                                                                                                                                                                                                                          <  2:988809/112‑1 (MQ=255)
                                                                                                                                                                                                                                         ccAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTcccc                                                                                                                                                                                                                          >  1:988809/1‑112 (MQ=255)
                                                                                                                                                                                                                                          cAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCagaaga                                                                               >  2:1910171/1‑250 (MQ=255)
                                                                                                                                                                                                                                            gAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGGAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGa                                       >  1:1048697/1‑288 (MQ=255)
                                                                                                                                                                                                                                            gAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGa                                       >  2:272885/1‑288 (MQ=255)
                                                                                                                                                                                                                                              aTACCGTTCCCAAGTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGACAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCa                                    <  1:1109106/289‑1 (MQ=255)
                                                                                                                                                                                                                                               tACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATCCAATGCTGAAGGTTTCATTGCGTAACCCCAGCCAGAAGTGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCACAGTTTCCCACCAGCATGGGGCCGCGAAAGGTGATGACATTGCCATGCCCCAGAAGACCGCACTGAATGGCGGTAAAATCCCTCTTTCTGCAAATGAGCa                                    >  2:1575767/1‑288 (MQ=255)
                                                                                                                                                                                                                                                  cGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACgg                                <  2:188399/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  cGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACgg                                >  2:275731/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTTCTCGGTAAAGGCGGTCAGCTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATAGCTATGTACGCAAATGAGCAAAggg                               >  1:608463/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATg                                                                                         <  1:1792376/230‑1 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCCCTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACggg                               >  1:1670545/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCAGTGAATGGCGGTAAAATCGCGATGTCAGCAAATGGGCAACggg                               >  2:293318/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    ttCTCAATTTTTGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTATGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACggg                               >  1:1910756/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               tGGCATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTgg                   <  2:1189279/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  cATCCACGGTGTACCGATCAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGa                <  2:901336/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aCGGTCTAACGATTAGTGAAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGACGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccac           <  1:896874/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                cGATCAGTGAAATCGGCATCGCAAGCTTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGtgcctg  <  1:484165/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
AACCGTGCGTTGTTCATGACCAAAATCGAGGCCGGTAATGAGTGGAATGCTCAGGCGGGAACGCAAAAACGCGTACACACTCTCCAGGTTGTAACCTGCGTCATAATCATTGGGCGTGCTGCCGCTAAAGCTACCGAGAATAATCGCCTTCTGACGTGGCAAAATTCCCGCATGATAGAGCTGCAACAGCATACGTTCGACCCGGAAAGGGTGCTCGTTAATATCTTCCAGCACCAGAATACCGTTCTCAATTTTTGGCATCCACGGTGTACCGATCAGACTAATCAGCATCGCAAGATTGCCTCCCCACAACGTGCCTTCGGCCCGACATGTCGGGCCTTCCCCTTGCCATTCAATGGTGAAGGTTTCATTGCGTAACGCCAGCCAGAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTG  >  CP042583/1048051‑1048611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A