Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	148	57.0	1973990	96.6%	1906874	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,486,664	G→A	G1202S (GGC→AGC)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,486,664	0	G	A	100.0%	130.5 / NA	38	G1202S (GGC→AGC)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/14); total (24/14)

AGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAA  >  NC_000913/1486530‑1486798

aGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTc                                                                                                                                   >  1:612402/1‑140 (MQ=255)

   aaaTCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAAATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCaaa                                                                                                                                >  2:561805/1‑140 (MQ=255)

        cTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCt                                                                                                                           <  1:129275/140‑1 (MQ=255)

               cAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGac                                                                                                                    <  2:488717/140‑1 (MQ=255)

                       aaTATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCg                                                                                                            >  2:553248/1‑140 (MQ=255)

                           tCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGatat                                                                                                        <  2:47770/140‑1 (MQ=255)

                            caacaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATAtt                                                                                                       >  1:629607/1‑140 (MQ=255)

                              acaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg                                                                                                     >  2:236223/1‑140 (MQ=255)

                              acaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg                                                                                                     >  2:633722/1‑140 (MQ=255)

                              acaaACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTg                                                                                                     >  2:152671/1‑140 (MQ=255)

                                aaaCGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCa                                                                                                   <  2:849630/140‑1 (MQ=255)

                                              gCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAAc                                                                                     >  1:553648/1‑140 (MQ=255)

                                                       tatGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGaaa                                                                            >  2:73974/1‑140 (MQ=255)

                                                            ccATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTGACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTg                                                                       >  2:684815/1‑140 (MQ=255)

                                                               tGGCGCGGGGGCGGGATGACAGAAAAGCGAAGAGGGACAGGGTGGAATATCTCGGGTGGGACACGGGTAACAGCTTGAAAAGTCGGGGCTACACGCTGCGATATTTGCAGGCGAGTGAAAAACGACGGGAAAAACTGGCg                                                                    >  2:849348/1‑140 (MQ=255)

                                                                ggCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCgg                                                                   <  2:868136/140‑1 (MQ=255)

                                                                ggCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCgg                                                                   <  2:443150/140‑1 (MQ=255)

                                                                    cTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGa                                                               >  1:493938/1‑140 (MQ=255)

                                                                      ggggCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                             <  1:553248/140‑1 (MQ=255)

                                                                            ttCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                             >  1:929476/1‑134 (MQ=255)

                                                                            ttCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATc                                                             <  2:929476/134‑1 (MQ=255)

                                                                             tCTGACATTAAAGCGCAAATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATcg                                                      <  1:561805/140‑1 (MQ=255)

                                                                                   aTTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTATCAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCg                                                <  1:605703/140‑1 (MQ=255)

                                                                                       aaGCGCAGATGGGCGGGTTGGTATGTCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTgcg                                            >  1:944239/1‑140 (MQ=255)

                                                                                               aTGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCt                                    >  2:859587/1‑140 (MQ=255)

                                                                                                          ggTATATCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGaaa                         <  1:73974/140‑1 (MQ=255)

                                                                                                            tataTCGCGGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGaaaaa                       >  1:189403/1‑139 (MQ=255)

                                                                                                                 cgcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCca                  >  1:663111/1‑140 (MQ=255)

                                                                                                                 cgcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAATCGAAAACGTccc                  >  2:864682/1‑139 (MQ=255)

                                                                                                                 cgcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGACGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCAGATCGCGACCCTGCACAGATGCTGAAAGTCGAAAATGTccc                  >  2:871149/1‑139 (MQ=255)

                                                                                                                  gcgGTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcag                 >  1:509419/1‑140 (MQ=255)

                                                                                                                     gTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcagcag              >  2:226279/1‑140 (MQ=255)

                                                                                                                     gTTTTGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCcagcag              >  1:792021/1‑140 (MQ=255)

                                                                                                                      ttttGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAgg             >  2:207207/1‑140 (MQ=255)

                                                                                                                      ttttGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTG‑AAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGc            >  1:365308/1‑140 (MQ=255)

                                                                                                                       tttGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGc            <  1:849348/140‑1 (MQ=255)

                                                                                                                         tGTCACTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGt          <  2:553648/140‑1 (MQ=255)

                                                                                                                             aCTGGTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGGTGGTTCCCAACCCGACCGGGGGGAGATGGTGGAAGGTGGAAAAGTCCAGCAGGCgggggg      >  2:839178/1‑138 (MQ=255)

                                                                                                                                 gTAACAGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCaa  <  1:839178/140‑1 (MQ=255)

AGCAAATCCTTACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTGGGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGTAACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTGGAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTCCAGCAGGCGTGGCAGCAA  >  NC_000913/1486530‑1486798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A