Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,130,739 A→G T182A (ACT→GCT)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,130,7390AG99.7% 971.0 / ‑5.4 309T182A (ACT→GCT) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (150/158);  total (151/158)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 315 total aligned reads displayed.

TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130649‑4130828
                                                                                          |                                                                                         
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  1:7859400/1‑93 (MQ=255)
 aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTc                                                                                        >  2:6796992/1‑93 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                       >  1:6199854/1‑93 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                       >  2:3286831/1‑93 (MQ=255)
   ccGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAg                                                                                      <  2:60973/93‑1 (MQ=255)
     ggAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGtt                                                                                    <  2:6336135/93‑1 (MQ=255)
      gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttc                                                                                   >  2:7780497/1‑93 (MQ=255)
      gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttc                                                                                   >  2:3190575/1‑93 (MQ=255)
       aaGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttct                                                                                  >  2:617461/1‑93 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                 >  2:882341/1‑93 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                <  1:16342/93‑1 (MQ=255)
         attCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                <  1:6467608/92‑1 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                               <  1:2789942/93‑1 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATATGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                               >  2:5438555/1‑93 (MQ=255)
           tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                              <  1:4560191/93‑1 (MQ=255)
             aCCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCg                                                                            <  1:1787554/93‑1 (MQ=255)
             aCCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCg                                                                            <  1:4995333/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  1:9827/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  1:7432792/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  2:1374171/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  1:5520278/93‑1 (MQ=255)
              cccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGa                                                                           <  1:3647878/93‑1 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                         <  1:5422902/93‑1 (MQ=255)
                 ggAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGt                                                                        <  1:67903/93‑1 (MQ=255)
                  gAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTc                                                                       >  2:6712420/1‑93 (MQ=255)
                   aaGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCg                                                                      >  2:763759/1‑93 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  >  2:6623625/1‑93 (MQ=255)
                        aaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 <  2:4130448/93‑1 (MQ=255)
                         aaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCt                                                                >  2:4783094/1‑93 (MQ=255)
                          aaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTa                                                               >  1:2158340/1‑93 (MQ=255)
                           aGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAc                                                              <  2:3470614/93‑1 (MQ=255)
                           aGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAc                                                              >  2:2119230/1‑93 (MQ=255)
                           aGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAc                                                              <  2:3632692/93‑1 (MQ=255)
                            gcgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                             <  2:2578491/93‑1 (MQ=255)
                             cgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCt                                                            >  2:7444360/1‑93 (MQ=255)
                             cgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCt                                                            <  1:3168957/93‑1 (MQ=255)
                              gcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTg                                                           >  1:5966955/1‑93 (MQ=255)
                              gcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTg                                                           <  2:1573882/93‑1 (MQ=255)
                               cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          <  2:5516269/93‑1 (MQ=255)
                               cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          <  2:6266738/93‑1 (MQ=255)
                               cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          >  2:4976003/1‑93 (MQ=255)
                                 cAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGt                                                        >  1:6967562/1‑93 (MQ=255)
                                  aGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtt                                                       <  2:4633797/93‑1 (MQ=255)
                                   ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGttt                                                      >  2:3804158/1‑93 (MQ=255)
                                   ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGttt                                                      <  1:7925756/93‑1 (MQ=255)
                                    gCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGACAGGTACCTGCGtttt                                                     >  1:6912048/1‑93 (MQ=255)
                                    gCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtttt                                                     >  1:729601/1‑93 (MQ=255)
                                    gCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGtttt                                                     <  2:6517331/93‑1 (MQ=255)
                                      ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCg                                                   >  2:3540201/1‑93 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                  >  1:3225578/1‑93 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                  >  2:1923317/1‑93 (MQ=255)
                                       gATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt                                                  <  1:2603608/93‑1 (MQ=255)
                                         tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                                >  1:2711051/1‑93 (MQ=255)
                                         tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                                >  1:5255592/1‑93 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              >  1:2431535/1‑93 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              >  2:5897865/1‑93 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              >  1:4417810/1‑93 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              <  1:5894729/93‑1 (MQ=255)
                                              ttAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCt                                           >  2:4861934/1‑93 (MQ=255)
                                                aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgt                                         <  1:424354/93‑1 (MQ=255)
                                                aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgt                                         <  2:2692576/93‑1 (MQ=255)
                                                aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgt                                         <  1:1037901/93‑1 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTATCTGCGTTTTCGTGACCGCTgtgt                                       >  2:5677327/1‑93 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       >  2:4198445/1‑93 (MQ=255)
                                                  tCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTgtgt                                       >  1:1280223/1‑93 (MQ=255)
                                                    tGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAt                                     >  1:2079406/1‑93 (MQ=255)
                                                     gAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATc                                    <  2:1159110/93‑1 (MQ=255)
                                                       aaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcgg                                  >  1:3424510/1‑93 (MQ=255)
                                                       aaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcgg                                  >  2:1292887/1‑93 (MQ=255)
                                                         cGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcg                                >  1:7505724/1‑93 (MQ=255)
                                                           cAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTCCCTGCGTTTTCGTGACCGCTGTGTATCGGCggg                              >  1:7625428/1‑93 (MQ=255)
                                                           cAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCggg                              >  2:7104446/1‑93 (MQ=255)
                                                            aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTAACCGCTGTGTATCGGCGGGc                             <  1:7121670/93‑1 (MQ=255)
                                                               gTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCgggggtt                          >  2:5134860/1‑89 (MQ=255)
                                                               gTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGAtt                          >  2:2918748/1‑93 (MQ=255)
                                                               gTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAtt                          <  1:3836819/93‑1 (MQ=255)
                                                                tGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTg                         <  2:6285212/93‑1 (MQ=255)
                                                                 ggATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                        <  2:1806464/93‑1 (MQ=255)
                                                                  gATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGAt                       >  2:7735925/1‑93 (MQ=255)
                                                                   aTGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCTGCGGGCATTGAtg                      >  2:1329309/1‑93 (MQ=255)
                                                                   aTGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtg                      <  1:6297260/93‑1 (MQ=255)
                                                                       cGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTGCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGa                  <  2:7483248/93‑1 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGTGTGGaaa                >  1:373491/1‑93 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaaa                <  1:6416007/93‑1 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaaaat              >  2:2392136/1‑91 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAatt              >  2:2811561/1‑93 (MQ=255)
                                                                            ccAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAatta             <  2:1653004/93‑1 (MQ=255)
                                                                            caaaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGGTTGGTGTGGTAatta             >  2:6427532/3‑93 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          >  2:6232814/1‑93 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          >  2:4003507/1‑93 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          <  1:2417913/93‑1 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTc          >  1:5135734/1‑93 (MQ=255)
                                                                                ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc         <  1:144589/93‑1 (MQ=255)
                                                                                  gcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTCTGTGGAAATTATTTCgg       >  2:5204253/1‑93 (MQ=255)
                                                                                  gcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCgg       >  1:1955517/1‑93 (MQ=255)
                                                                                  gcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCgg       <  1:112600/93‑1 (MQ=255)
                                                                                  gcgcgATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCgg       <  1:7045214/93‑1 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGa     >  1:4744647/1‑93 (MQ=255)
                                                                                     cgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGaa    <  2:6953479/93‑1 (MQ=255)
                                                                                       aTTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGGGGAAATTATTTCTGGGAtt  >  2:4543604/1‑93 (MQ=255)
                                                                                          |                                                                                         
TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130649‑4130828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  NC_000913/4130646‑4130832
                                                                                             |                                                                                             
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTG                                                                                               <  SRR4302543.3151906/94‑1 (MQ=60)
 CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                              >  SRR4302543.4375183/1‑94 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCACCCGCGCGATTGCT                                                                                             >  SRR4302543.5876092/1‑94 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             <  SRR4302543.920942/94‑1 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                             >  SRR4302543.7933432/1‑94 (MQ=60)
    ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCA                                                                                           >  SRR4302543.6258416/1‑94 (MQ=60)
    ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCA                                                                                           >  SRR4302543.6485775/1‑94 (MQ=60)
           AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                    >  SRR4302543.1542118/1‑94 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   >  SRR4302543.7936210/1‑94 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302543.7830367/94‑1 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302543.16495/94‑1 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302543.7132105/94‑1 (MQ=60)
            ATTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                   <  SRR4302543.6528541/94‑1 (MQ=60)
             TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTT                                                                                  <  SRR4302543.2815411/94‑1 (MQ=60)
              TCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTC                                                                                 <  SRR4302543.4601701/94‑1 (MQ=60)
                ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               <  SRR4302543.1803543/94‑1 (MQ=60)
                ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               <  SRR4302543.5040802/94‑1 (MQ=60)
                ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               <  SRR4302543.5040803/94‑1 (MQ=60)
                ACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATACCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGA                                                                               <  SRR4302543.3705931/94‑1 (MQ=60)
                 CCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                              <  SRR4302543.9916/94‑1 (MQ=60)
                 CCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                              <  SRR4302543.5570418/94‑1 (MQ=60)
                 CCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                              <  SRR4302543.7504047/94‑1 (MQ=60)
                 CCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                              <  SRR4302543.3679945/94‑1 (MQ=60)
                  CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                             <  SRR4302543.7199691/94‑1 (MQ=60)
                   CGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGT                                                                            <  SRR4302543.5472200/94‑1 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302543.68509/94‑1 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           <  SRR4302543.4482860/94‑1 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           >  SRR4302543.3485878/1‑94 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                           >  SRR4302543.6019708/1‑94 (MQ=60)
                     GAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTTG                                                                          >  SRR4302543.1184140/1‑94 (MQ=60)
                      AAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGA                                                                         >  SRR4302543.7811535/1‑94 (MQ=60)
                      AAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGA                                                                         >  SRR4302543.6298354/1‑94 (MQ=60)
                       AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                        <  SRR4302543.1143906/94‑1 (MQ=60)
                         CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                      >  SRR4302543.3379347/1‑94 (MQ=60)
                          AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                     >  SRR4302543.7154810/1‑94 (MQ=60)
                           AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCT                                                                    <  SRR4302543.3383936/94‑1 (MQ=60)
                           AAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCT                                                                    >  SRR4302543.2511826/1‑94 (MQ=60)
                            AAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTA                                                                   >  SRR4302543.2177670/1‑94 (MQ=60)
                             AAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAC                                                                  <  SRR4302543.891690/94‑1 (MQ=60)
                             AAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAC                                                                  >  SRR4302543.7588624/1‑94 (MQ=60)
                             AAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTAC                                                                  <  SRR4302543.4042323/94‑1 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                               >  SRR4302543.6021592/1‑94 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                               <  SRR4302543.4076508/94‑1 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                               <  SRR4302543.3197492/94‑1 (MQ=60)
                                  CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                             >  SRR4302543.5243537/1‑94 (MQ=60)
                                  CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                             >  SRR4302543.5462717/1‑94 (MQ=60)
                                  CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                             <  SRR4302543.5276737/94‑1 (MQ=60)
                                   TCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGT                                                            >  SRR4302543.7033129/1‑94 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGACAGGTACCTGCGTTTT                                                         >  SRR4302543.6977018/1‑94 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         >  SRR4302543.736148/1‑94 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         <  SRR4302543.8000184/94‑1 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         <  SRR4302543.6339946/94‑1 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         >  SRR4302543.4741429/1‑94 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         >  SRR4302543.179985/1‑94 (MQ=60)
                                      GGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTT                                                         >  SRR4302543.1499338/1‑94 (MQ=60)
                                       GCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTC                                                        <  SRR4302543.4458579/94‑1 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                       <  SRR4302543.4408675/94‑1 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                       >  SRR4302543.2442636/1‑94 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                       <  SRR4302543.6008638/94‑1 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302543.3252550/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302543.7989627/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302543.2560811/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302543.3254738/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302543.5550055/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                      >  SRR4302543.6504901/1‑94 (MQ=60)
                                          GATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTG                                                     <  SRR4302543.2627255/94‑1 (MQ=60)
                                          GATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTG                                                     <  SRR4302543.6098613/94‑1 (MQ=60)
                                           ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302543.2735674/1‑94 (MQ=60)
                                           ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    <  SRR4302543.6719405/94‑1 (MQ=60)
                                           ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                    >  SRR4302543.5302994/1‑94 (MQ=60)
                                            TTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAC                                                   <  SRR4302543.5536420/94‑1 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  >  SRR4302543.2453477/1‑94 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  >  SRR4302543.4457854/1‑94 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                  <  SRR4302543.3243875/94‑1 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                                 <  SRR4302543.7519694/94‑1 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                                 >  SRR4302543.2265648/1‑94 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGACTTCGAAACCTACCTGatttttgctgaacc                                                 >  SRR4302543.2265649/1‑80 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                                 <  SRR4302543.5948483/94‑1 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                                >  SRR4302543.3959409/1‑94 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                                >  SRR4302543.6991068/1‑94 (MQ=60)
                                                  TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                             <  SRR4302543.5418014/94‑1 (MQ=60)
                                                   AATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTG                                            <  SRR4302543.428326/94‑1 (MQ=60)
                                                   AATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTG                                            <  SRR4302543.1047133/94‑1 (MQ=60)
                                                   AATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTG                                            <  SRR4302543.6492342/94‑1 (MQ=60)
                                                    ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302543.2780066/1‑94 (MQ=60)
                                                    ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302543.1291634/1‑94 (MQ=60)
                                                    ATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGT                                           >  SRR4302543.2780068/1‑94 (MQ=60)
                                                      CTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAT                                         <  SRR4302543.5463462/94‑1 (MQ=60)
                                                      CTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAT                                         >  SRR4302543.2097972/1‑94 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGCTTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302543.4876993/94‑1 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                       <  SRR4302543.3855008/94‑1 (MQ=60)
                                                         AAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGG                                      >  SRR4302543.3455152/1‑94 (MQ=60)
                                                          AACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGC                                     <  SRR4302543.1983714/94‑1 (MQ=60)
                                                           ACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCG                                    >  SRR4302543.1842882/1‑94 (MQ=60)
                                                           ACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCG                                    <  SRR4302543.3161453/94‑1 (MQ=60)
                                                           ACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCG                                    >  SRR4302543.7577468/1‑94 (MQ=60)
                                                            CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                   <  SRR4302543.1405267/94‑1 (MQ=60)
                                                             GCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTCCCTGCGTTTTCGTGACCGCTGTGTATCGGCGGG                                  >  SRR4302543.7697789/1‑94 (MQ=60)
                                                             GCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGG                                  <  SRR4302543.5044164/94‑1 (MQ=60)
                                                             GCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGG                                  >  SRR4302543.3814296/1‑94 (MQ=60)
                                                              CAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGC                                 <  SRR4302543.2448675/94‑1 (MQ=60)
                                                               AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCA                                >  SRR4302543.6380862/1‑94 (MQ=60)
                                                               AAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTAACCGCTGTGTATCGGCGGGCA                                <  SRR4302543.7190856/94‑1 (MQ=60)
                                                                AAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAT                               <  SRR4302543.6869605/94‑1 (MQ=60)
                                                                AAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAT                               <  SRR4302543.1970298/94‑1 (MQ=60)
                                                                AAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAT                               >  SRR4302543.7202220/1‑94 (MQ=60)
                                                                AAGTGGAGGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAT                               <  SRR4302543.2355505/94‑1 (MQ=60)
                                                                 AGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATT                              >  SRR4302543.1446802/1‑94 (MQ=60)
                                                                 AGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATT                              <  SRR4302543.1691222/94‑1 (MQ=60)
                                                                  GTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTG                             >  SRR4302543.1100989/1‑94 (MQ=60)
                                                                  GTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTG                             <  SRR4302543.3870681/94‑1 (MQ=60)
                                                                  GTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTG                             <  SRR4302543.319427/94‑1 (MQ=60)
                                                                    GGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT                           <  SRR4302543.4410639/94‑1 (MQ=60)
                                                                     GATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCACTGATT                          >  SRR4302543.3744686/1‑94 (MQ=60)
                                                                      ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGCTTGATGT                         >  SRR4302543.266945/1‑94 (MQ=60)
                                                                      ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT                         <  SRR4302543.6356529/94‑1 (MQ=60)
                                                                         CCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGA                      >  SRR4302543.6919666/1‑94 (MQ=60)
                                                                         CCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGA                      <  SRR4302543.5110499/94‑1 (MQ=60)
                                                                         CCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGA                      >  SRR4302543.7007520/1‑94 (MQ=60)
                                                                           GGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGTGTGGAAA                    >  SRR4302543.376930/1‑94 (MQ=60)
                                                                           GGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAA                    >  SRR4302543.4632298/1‑94 (MQ=60)
                                                                            GAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAAT                   <  SRR4302543.6476447/94‑1 (MQ=60)
                                                                              GCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTT                 >  SRR4302543.2140446/1‑94 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302543.1085158/94‑1 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302543.322/94‑1 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302543.615116/94‑1 (MQ=60)
                                                                               CCAACCGCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT                <  SRR4302543.7533931/94‑1 (MQ=60)
                                                                                CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATT               >  SRR4302543.6747909/1‑94 (MQ=60)
                                                                                CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATT               <  SRR4302543.7265551/94‑1 (MQ=60)
                                                                                 AACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTC              >  SRR4302543.5182157/1‑94 (MQ=60)
                                                                                  ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAactttccctcgcttttttttttgcgccgcttttgtgtggtgggggggtgtgtgtttgt             >  SRR4302543.6399975/1‑36 (MQ=60)
                                                                                  ACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCC             <  SRR4302543.2439752/94‑1 (MQ=60)
                                                                                   CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            <  SRR4302543.145846/94‑1 (MQ=60)
                                                                                   CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCG            <  SRR4302543.7418145/94‑1 (MQ=60)
                                                                                    CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGTGGGCATTGATGTGGAAATTATTCCGG           <  SRR4302543.2956634/94‑1 (MQ=60)
                                                                                    CGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCGG           >  SRR4302543.1973058/1‑94 (MQ=60)
                                                                                     GCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGG          <  SRR4302543.199756/94‑1 (MQ=60)
                                                                                     GCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGG          <  SRR4302543.113624/94‑1 (MQ=60)
                                                                                     GCGCGATCGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGG          <  SRR4302543.7113418/94‑1 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCCGGA         >  SRR4302543.7481261/1‑94 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGA         >  SRR4302543.4788007/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTTGTTTGGGAATTATTCCGGGGAT       >  SRR4302543.2150540/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATTTGGAAATTATTCCGGGAAT       >  SRR4302543.6459390/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTTCGGGGAT       >  SRR4302543.7666883/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT       >  SRR4302543.2729998/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT       >  SRR4302543.932876/1‑94 (MQ=60)
                                                                                         GATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT      <  SRR4302543.5689704/94‑1 (MQ=60)
                                                                                         GATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT      <  SRR4302543.2048262/94‑1 (MQ=60)
                                                                                          ATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGGATTT     >  SRR4302543.3455268/1‑94 (MQ=60)
                                                                                          ATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTT     >  SRR4302543.6930388/1‑94 (MQ=60)
                                                                                          ATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTT     <  SRR4302543.3678467/94‑1 (MQ=60)
                                                                                            TGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGCAGTGGAAATTATTCCGGGGATTTTG   >  SRR4302543.3796678/1‑94 (MQ=60)
                                                                                             GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGCTTGATGTGGAAATTATTCCGGGAATTTTGC  >  SRR4302543.924314/1‑94 (MQ=60)
                                                                                             GCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATGATTCCGGGGATTTTGC  >  SRR4302543.7099876/1‑94 (MQ=60)
                                                                                             GCTCAGTTCTTCTTCGATGTAGAAAGCTACCTGCGTTTTCTTGACCGCTGTtgattggcgggggtgttggagggaattgtatacggggtttttg  >  SRR4302543.7099875/1‑51 (MQ=60)
                                                                                             |                                                                                             
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTTGC  >  NC_000913/4130646‑4130832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑