Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,134,509 A→G E274E (GAA→GAG)  b3943 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,134,5090AG99.1% 1005.4 / ‑1.8 321E274E (GAA→GAG) b3943hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (138/180);  total (138/183)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

Only 100 of 334 total aligned reads displayed.

CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598
                                                                                          |                                                                                         
  gTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTa                                                                                       <  1:7128906/93‑1 (MQ=255)
  gTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTa                                                                                       >  1:3231323/1‑93 (MQ=255)
  gTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTa                                                                                       <  2:6891625/93‑1 (MQ=255)
  gTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTa                                                                                       >  1:822425/1‑93 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     <  1:7410685/93‑1 (MQ=255)
        ccAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTa                                                                                 >  1:7282872/1‑93 (MQ=255)
        ccAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTa                                                                                 <  1:6234469/93‑1 (MQ=255)
        ccAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGCAGCggga                                                                                 >  2:2237051/1‑91 (MQ=255)
          aGCTTAAGCACACTGGCCGTTCCCGTTTGCGTCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGCGGGCCGGTGCGGGTAGCGGCatt                                                                               >  1:5963819/1‑93 (MQ=255)
           gCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattg                                                                              >  1:1410341/1‑93 (MQ=255)
            cTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgt                                                                             <  2:2843184/93‑1 (MQ=255)
             ttAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtg                                                                            >  2:14059/1‑93 (MQ=255)
             ttAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtg                                                                            >  1:6853248/1‑93 (MQ=255)
              tAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgc                                                                           <  2:6460531/93‑1 (MQ=255)
              tAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgc                                                                           <  1:2885138/93‑1 (MQ=255)
                 gCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctga                                                                        <  2:6289958/93‑1 (MQ=255)
                 gCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctga                                                                        <  1:6178953/93‑1 (MQ=255)
                 gCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctga                                                                        >  2:4664358/1‑93 (MQ=255)
                  cacaCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgat                                                                       <  2:6837531/93‑1 (MQ=255)
                    cacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattg                                                                     >  1:1771816/1‑93 (MQ=255)
                    cacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattg                                                                     <  2:2708183/93‑1 (MQ=255)
                    cacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattg                                                                     >  1:4854322/1‑93 (MQ=255)
                     ccTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgt                                                                    <  1:5556269/92‑1 (MQ=255)
                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  <  2:5673768/93‑1 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 <  1:1190235/93‑1 (MQ=255)
                         gCCGTTCCCGTTTGCGGAGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgctg                                                                <  2:2512524/93‑1 (MQ=255)
                           ctttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              <  1:1621187/91‑1 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              >  1:2596886/1‑93 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              <  1:5755107/93‑1 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATACGGGGGCCGTTGAGGTCAGCGGGATTGTGCTCATTGTGCTGGc                                                              >  2:2191958/1‑93 (MQ=255)
                            gTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCa                                                             >  2:4746462/1‑93 (MQ=255)
                            gTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCa                                                             >  2:845957/1‑93 (MQ=255)
                            gTTCCCGTGTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCa                                                             <  1:1043019/93‑1 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:5109743/1‑93 (MQ=255)
                              tCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAcg                                                           >  2:5528856/1‑92 (MQ=255)
                              tCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACt                                                           <  2:5985634/93‑1 (MQ=255)
                              tCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACt                                                           <  1:4043622/93‑1 (MQ=255)
                                 cGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgc                                                        >  1:5861426/1‑93 (MQ=255)
                                  gTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgcg                                                       >  2:3499452/1‑93 (MQ=255)
                                   tttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgcgc                                                      >  2:1900087/1‑93 (MQ=255)
                                   tttGCGGCGTACTCTTTTCCTGGGGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGAGCTGGCACTGgcgt                                                      >  2:5440101/1‑92 (MQ=255)
                                     tGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTg                                                    >  2:3202993/1‑93 (MQ=255)
                                       cggcgTACTCTTTTCCTGGAGGCTGCTCGGCGAGAATCCGGGGGCCGATGAGGGTAGCGGTATTGTGCTGATTGAGCTGGCACTGGCGCTGGt                                                  >  2:3553687/1‑93 (MQ=255)
                                        ggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 <  2:3334697/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:5706530/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:2491220/93‑1 (MQ=255)
                                         gcgTACTCTTTTCCTGATGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:443616/93‑1 (MQ=255)
                                          ggttctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAg                                               <  1:1990939/89‑1 (MQ=255)
                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGc                                              <  1:4569371/93‑1 (MQ=255)
                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGc                                              >  2:1667346/1‑93 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  2:2347943/1‑93 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  2:7769209/1‑93 (MQ=255)
                                             aCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            >  1:110607/1‑93 (MQ=255)
                                               tctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTa                                          >  1:2773208/1‑93 (MQ=255)
                                               tctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTa                                          >  1:2704301/1‑93 (MQ=255)
                                                ctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         <  2:7312589/93‑1 (MQ=255)
                                                ctTTGCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         <  2:2116693/93‑1 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        <  2:7158547/93‑1 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        >  2:3835107/1‑93 (MQ=255)
                                                  tttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCTTAAGa                                       <  1:550214/93‑1 (MQ=255)
                                                     ccTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaa                                    <  1:3818599/93‑1 (MQ=255)
                                                     ccTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaa                                    <  1:4709101/93‑1 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   >  1:4948401/1‑93 (MQ=255)
                                                       tggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaaa                                  <  2:1021316/93‑1 (MQ=255)
                                                        ggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaag                                 <  2:2654633/93‑1 (MQ=255)
                                                         gtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga                                >  1:2121359/1‑93 (MQ=255)
                                                         gtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga                                >  1:4375202/1‑93 (MQ=255)
                                                           ggCTGCTCGGCGAGAATCCGGGGGCTGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCCCTGGCGCTGGTGAGCCGTAAGAAAAaagaag                              <  2:1951425/93‑1 (MQ=255)
                                                           ggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaag                              <  2:4044636/93‑1 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             >  2:3047201/1‑93 (MQ=255)
                                                             ctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  2:3661135/93‑1 (MQ=255)
                                                              tgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCg                           >  1:662793/1‑93 (MQ=255)
                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          <  1:3486890/93‑1 (MQ=255)
                                                                ctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc                         <  2:4943961/93‑1 (MQ=255)
                                                                  cGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAg                       <  1:523404/93‑1 (MQ=255)
                                                                   ggCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgt                      <  1:2499776/93‑1 (MQ=255)
                                                                    gCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgtg                     >  2:895082/1‑93 (MQ=255)
                                                                      gagaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTa                   <  2:7846723/93‑1 (MQ=255)
                                                                      gagaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTa                   >  1:6933862/1‑93 (MQ=255)
                                                                       agaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaa                  >  1:3913836/1‑93 (MQ=255)
                                                                       agaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaa                  <  2:7614860/93‑1 (MQ=255)
                                                                        gaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaa                 >  1:354846/1‑93 (MQ=255)
                                                                        gaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaa                 <  1:3025021/93‑1 (MQ=255)
                                                                          aTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaaa               <  1:2264888/93‑1 (MQ=255)
                                                                          aTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaaa               <  2:6711498/93‑1 (MQ=255)
                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  1:7788976/93‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:4610989/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          <  2:3901291/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          <  1:3158986/93‑1 (MQ=255)
                                                                                 ggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTg        <  2:3140083/93‑1 (MQ=255)
                                                                                   ccGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGaa      <  1:341846/93‑1 (MQ=255)
                                                                                   ccGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGaa      <  1:6339342/93‑1 (MQ=255)
                                                                                   ccGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGaa      <  2:4889001/93‑1 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:707234/93‑1 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:4455760/93‑1 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:5254788/93‑1 (MQ=255)
                                                                                     gTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAtt    <  2:6130876/93‑1 (MQ=255)
                                                                                     gTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAtt    >  1:4437807/1‑93 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAATAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  1:7653920/1‑93 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  1:2063253/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  >  NC_000913/4134416‑4134602
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               <  SRR4302543.7621741/94‑1 (MQ=60)
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               >  SRR4302543.7840703/1‑94 (MQ=60)
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               >  SRR4302543.1506706/1‑94 (MQ=60)
TGCCAGTATTCCCAGCTTAAGCCCACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               <  SRR4302543.6355838/94‑1 (MQ=60)
 GCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGG                                                                                              >  SRR4302543.720921/1‑94 (MQ=60)
 GCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGG                                                                                              <  SRR4302543.6189886/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             >  SRR4302543.3483291/1‑94 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGT                                                                                            <  SRR4302543.3002344/94‑1 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302543.3260544/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           <  SRR4302543.3823488/94‑1 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302543.839005/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302543.829674/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302543.6613929/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302543.4809721/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302543.1178445/1‑94 (MQ=60)
     attgttccatgcTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGTCGTTGAGGGTAG                                                                                          <  SRR4302543.1667999/82‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          <  SRR4302543.1667998/94‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          >  SRR4302543.1491975/1‑94 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          <  SRR4302543.3397012/94‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAG                                                                                          <  SRR4302543.7198183/94‑1 (MQ=60)
     GTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGCAG                                                                                          >  SRR4302543.1880536/1‑94 (MQ=60)
      TATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGC                                                                                         <  SRR4302543.6043814/94‑1 (MQ=60)
      TATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGC                                                                                         >  SRR4302543.6503149/1‑94 (MQ=60)
      TATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGC                                                                                         >  SRR4302543.7295454/1‑94 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        <  SRR4302543.7481791/94‑1 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        <  SRR4302543.1299243/94‑1 (MQ=60)
         TGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGT                                                                                      <  SRR4302543.6158778/94‑1 (MQ=60)
         TGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGT                                                                                      <  SRR4302543.7473813/94‑1 (MQ=60)
          GCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTA                                                                                     >  SRR4302543.7353198/1‑94 (MQ=60)
           CCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTAT                                                                                    >  SRR4302543.4958834/1‑94 (MQ=60)
           CCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTAT                                                                                    <  SRR4302543.6293310/94‑1 (MQ=60)
            CAGCTTAAGCACACTGGCCGTTCCCGTTTGCGTCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGCGGGCCGGTGCGGGTAGCGGCATT                                                                                   >  SRR4302543.6018428/1‑94 (MQ=60)
            CAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATT                                                                                   <  SRR4302543.5811660/94‑1 (MQ=60)
             AGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTG                                                                                  >  SRR4302543.3712309/1‑94 (MQ=60)
             AGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTG                                                                                  >  SRR4302543.1423085/1‑94 (MQ=60)
              GCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGT                                                                                 <  SRR4302543.2955732/94‑1 (MQ=60)
              GCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGT                                                                                 <  SRR4302543.7882293/94‑1 (MQ=60)
               CTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTG                                                                                >  SRR4302543.6917879/1‑94 (MQ=60)
                TTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTGGCGGTATTGTGT                                                                               >  SRR4302543.2053810/1‑94 (MQ=60)
                 TAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCT                                                                              <  SRR4302543.2911393/94‑1 (MQ=60)
                  AAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTG                                                                             <  SRR4302543.7761681/94‑1 (MQ=60)
                  AAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTG                                                                             <  SRR4302543.2146694/94‑1 (MQ=60)
                  AAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTG                                                                             >  SRR4302543.2678700/1‑94 (MQ=60)
                    GCACACTGGCCGTTCCCGTTTGCGGCGTGCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGAT                                                                           <  SRR4302543.108245/94‑1 (MQ=60)
                    GCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGAT                                                                           <  SRR4302543.6922731/94‑1 (MQ=60)
                    GCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGAT                                                                           <  SRR4302543.6237365/94‑1 (MQ=60)
                     CACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATT                                                                          <  SRR4302543.7399524/94‑1 (MQ=60)
                     CACACTGGCCGTGCCCGTTTGCGGCGTACTCTTATCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATT                                                                          <  SRR4302543.7678132/94‑1 (MQ=60)
                      ACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         >  SRR4302543.1787654/1‑94 (MQ=60)
                      ACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         <  SRR4302543.4459618/94‑1 (MQ=60)
                      ACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         >  SRR4302543.4898587/1‑94 (MQ=60)
                       CACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGT                                                                        >  SRR4302543.2610034/1‑94 (MQ=60)
                        CCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTG                                                                       <  SRR4302543.6285310/94‑1 (MQ=60)
                        CCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTG                                                                       <  SRR4302543.5606801/94‑1 (MQ=60)
                         CTGGCCGTGCCCGTTTGCGGCGTACTCTTTGCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGC                                                                      <  SRR4302543.2231945/94‑1 (MQ=60)
                           GGCCGTTCGCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCGCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302543.5580822/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302543.3892053/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302543.1202942/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302543.3309294/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302543.1200969/94‑1 (MQ=60)
                            GCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGG                                                                   <  SRR4302543.3957485/94‑1 (MQ=60)
                             CCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGC                                                                  >  SRR4302543.2620477/1‑94 (MQ=60)
                             CCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGC                                                                  >  SRR4302543.2620478/1‑94 (MQ=60)
                              CTTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 <  SRR4302543.1635635/94‑1 (MQ=60)
                              CGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 >  SRR4302543.5898979/1‑94 (MQ=60)
                              CGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 <  SRR4302543.5807553/94‑1 (MQ=60)
                              CGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCA                                                                 >  SRR4302543.6249806/1‑94 (MQ=60)
                               GTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAC                                                                >  SRR4302543.5004668/1‑94 (MQ=60)
                               GTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAC                                                                >  SRR4302543.460792/1‑94 (MQ=60)
                               GTTCCCGTGTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCAC                                                                <  SRR4302543.1052321/94‑1 (MQ=60)
                                 TCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTG                                                              <  SRR4302543.4079812/94‑1 (MQ=60)
                                  CCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGG                                                             <  SRR4302543.4700647/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302543.5427098/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            >  SRR4302543.5914770/1‑94 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302543.3233556/94‑1 (MQ=60)
                                    CGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGCCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCC                                                           >  SRR4302543.5679668/1‑94 (MQ=60)
                                    CGTTTGCGCCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCG                                                           <  SRR4302543.6197579/94‑1 (MQ=60)
                                      TTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCT                                                         <  SRR4302543.3300730/94‑1 (MQ=60)
                                        TGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGG                                                       >  SRR4302543.2121415/1‑94 (MQ=60)
                                          CGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     <  SRR4302543.1844644/94‑1 (MQ=60)
                                             ggttCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGC                                                  <  SRR4302543.2008785/90‑1 (MQ=60)
                                             CGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGC                                                  >  SRR4302543.6958847/1‑94 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 <  SRR4302543.4610927/94‑1 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 >  SRR4302543.4486788/1‑94 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGGATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 >  SRR4302543.259886/1‑94 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                >  SRR4302543.4980530/1‑94 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                <  SRR4302543.4755377/94‑1 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                >  SRR4302543.111613/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              <  SRR4302543.3058394/94‑1 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302543.1193672/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302543.7527907/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302543.2728841/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302543.2798454/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302543.2798455/1‑94 (MQ=60)
                                                 CTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTA                                              >  SRR4302543.2798456/1‑94 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302543.7578431/94‑1 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302543.5710075/94‑1 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            >  SRR4302543.3555057/1‑94 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302543.3741520/94‑1 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302543.1006383/94‑1 (MQ=60)
                                                   CTTTATCTGGTGGCTGCGCAGCGAGAATCCGGGTTACGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            <  SRR4302543.3738733/94‑1 (MQ=60)
                                                    TTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGA                                           <  SRR4302543.2310097/94‑1 (MQ=60)
                                                     TTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCTTAAGAA                                          <  SRR4302543.555280/94‑1 (MQ=60)
                                                     TTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAA                                          >  SRR4302543.3010408/1‑94 (MQ=60)
                                                      TTCCTGGTGGCTGCTCGGCGAGAATCCGTGGGCCGTTGAGGGGAGAGGTATTGTGCAGATTGTGCTGGTCCTGGCGCGCGAGAGCCGTAAGACA                                         >  SRR4302543.4575029/1‑94 (MQ=40)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       >  SRR4302543.4993461/1‑94 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       <  SRR4302543.3852273/94‑1 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGCAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       >  SRR4302543.6857368/1‑94 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       <  SRR4302543.4752143/94‑1 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGA                                    >  SRR4302543.2140353/1‑94 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGA                                    <  SRR4302543.1285487/94‑1 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGA                                    >  SRR4302543.4414842/1‑94 (MQ=60)
                                                           GGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGGAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTGAGAAAAAAGA                                    >  SRR4302543.2727268/1‑94 (MQ=60)
                                                            GTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAA                                   <  SRR4302543.1980006/94‑1 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  >  SRR4302543.5079219/1‑94 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302543.2098402/94‑1 (MQ=60)
                                                             GGGCTGCTCGGCGAGAATCCGGGGCCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGACACTGGCTCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302543.7773441/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302543.5401978/94‑1 (MQ=60)
                                                                GTGCTCGGGGAGAATCGGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCG                               <  SRR4302543.5966377/94‑1 (MQ=60)
                                                                CTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCG                               >  SRR4302543.668890/1‑94 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGTCGTAAGAAAAAAGAAGacga                              >  SRR4302543.4053085/1‑90 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGT                              >  SRR4302543.894904/1‑94 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGT                              >  SRR4302543.3239663/1‑94 (MQ=60)
                                                                  GCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC                             <  SRR4302543.1753102/94‑1 (MQ=60)
                                                                  GCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC                             >  SRR4302543.4629683/1‑94 (MQ=60)
                                                                  GCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTC                             <  SRR4302543.3518110/94‑1 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302543.2905068/1‑94 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302543.6412828/1‑94 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            <  SRR4302543.3870471/94‑1 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302543.7192997/1‑94 (MQ=60)
                                                                    TCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAG                           <  SRR4302543.6368143/94‑1 (MQ=60)
                                                                    TCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAG                           <  SRR4302543.6368144/94‑1 (MQ=60)
                                                                     CGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGT                          <  SRR4302543.528253/94‑1 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTTTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302543.1094869/94‑1 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302543.4476051/94‑1 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302543.4196597/94‑1 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302543.7215670/94‑1 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302543.2522462/94‑1 (MQ=60)
                                                                      GACGAGAACCCCGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302543.6894088/94‑1 (MQ=60)
                                                                        CGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTA                       >  SRR4302543.6999087/1‑94 (MQ=60)
                                                                         GAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAA                      >  SRR4302543.3948386/1‑94 (MQ=60)
                                                                          AGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAA                     >  SRR4302543.358123/1‑94 (MQ=60)
                                                                          AGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAA                     <  SRR4302543.711412/94‑1 (MQ=60)
                                                                          AGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGCAAGAAAAAAGAAGCCGTCAGTGTAAA                     >  SRR4302543.349700/1‑94 (MQ=60)
                                                                           GAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAA                    <  SRR4302543.3052358/94‑1 (MQ=60)
                                                                             ATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAG                  <  SRR4302543.2285561/94‑1 (MQ=60)
                                                                             ATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAG                  >  SRR4302543.4214547/1‑94 (MQ=60)
                                                                              TCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGG                 <  SRR4302543.7862394/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGA                <  SRR4302543.3568348/94‑1 (MQ=60)
                                                                               CCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGA                <  SRR4302543.5106282/94‑1 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               <  SRR4302543.5642587/94‑1 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               >  SRR4302543.2243607/1‑94 (MQ=60)
                                                                                 GGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCGCTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATC              <  SRR4302543.4736068/94‑1 (MQ=60)
                                                                                 GGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATC              >  SRR4302543.4367062/1‑94 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302543.3187426/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302543.3187425/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302543.290391/94‑1 (MQ=60)
                                                                                   GGGCCGTTGAGGGTGGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTG            <  SRR4302543.1544288/94‑1 (MQ=60)
                                                                                    GGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGA           >  SRR4302543.2366502/1‑94 (MQ=60)
                                                                                     GCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAA          <  SRR4302543.2055075/94‑1 (MQ=60)
                                                                                      CCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAT         <  SRR4302543.344993/94‑1 (MQ=60)
                                                                                      CCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAT         <  SRR4302543.6398950/94‑1 (MQ=60)
                                                                                       CGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATT        >  SRR4302543.4477997/1‑94 (MQ=60)
                                                                                        GTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAATAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTT       >  SRR4302543.7726486/1‑94 (MQ=60)
                                                                                        GTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTT       >  SRR4302543.2081707/1‑94 (MQ=60)
                                                                                        GTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTT       >  SRR4302543.7961897/1‑94 (MQ=60)
                                                                                          TGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTT     <  SRR4302543.5599479/94‑1 (MQ=60)
                                                                                            AGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTC   >  SRR4302543.5254320/1‑94 (MQ=60)
                                                                                             GGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  <  SRR4302543.4173504/94‑1 (MQ=60)
                                                                                             GGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  <  SRR4302543.576799/94‑1 (MQ=60)
                                                                                             GGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  <  SRR4302543.415859/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  >  NC_000913/4134416‑4134602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑