Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,138,157 A→G F427L (TTC→CTC)  ptsA ← PEP‑protein phosphotransferase system enzyme I/IIA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,138,1570AG99.0% 1278.4 / ‑0.8 403F427L (TTC→CTC) ptsAPEP‑protein phosphotransferase system enzyme I/IIA
Reads supporting (aligned to +/- strand):  ref base A (4/0);  new base G (231/168);  total (235/168)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 406 total aligned reads displayed.

TATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCG  >  NC_000913/4138067‑4138246
                                                                                          |                                                                                         
tATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCa                                                                                         >  2:4531448/1‑93 (MQ=255)
tATCGCGATCCACCGCCAGCAAATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCa                                                                                         <  2:7549980/93‑1 (MQ=255)
  tCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATc                                                                                       <  2:4612727/93‑1 (MQ=255)
  tCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATc                                                                                       >  1:4442487/1‑93 (MQ=255)
   cgcgATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCa                                                                                      >  2:7299859/1‑93 (MQ=255)
    gcgATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCAc                                                                                     >  1:3679856/1‑93 (MQ=255)
       aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGa                                                                                  >  2:5882845/1‑93 (MQ=255)
       aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGa                                                                                  >  2:1636986/1‑93 (MQ=255)
       aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGa                                                                                  <  1:7863022/93‑1 (MQ=255)
        tCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGAc                                                                                 >  1:766128/1‑93 (MQ=255)
        tCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGAc                                                                                 >  1:7477827/1‑93 (MQ=255)
           cccGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGc                                                                              <  1:2952544/92‑1 (MQ=255)
           aCCGCAAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGc                                                                              >  1:1478033/1‑93 (MQ=255)
              gCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACt                                                                           >  1:1058301/1‑93 (MQ=255)
              gCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACt                                                                           <  1:7787955/93‑1 (MQ=255)
                cagcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTc                                                                         <  2:2657048/93‑1 (MQ=255)
                  gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCa                                                                       >  1:5607669/1‑93 (MQ=255)
                  gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCa                                                                       >  2:4187533/1‑93 (MQ=255)
                  gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCa                                                                       <  1:4952696/93‑1 (MQ=255)
                  gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCa                                                                       <  1:3594639/93‑1 (MQ=255)
                   cagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAg                                                                      <  1:4655703/93‑1 (MQ=255)
                    agATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGc                                                                     >  2:4668757/1‑93 (MQ=255)
                     gATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCa                                                                    >  2:2099680/1‑93 (MQ=255)
                     gATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCa                                                                    <  2:2983698/93‑1 (MQ=255)
                      aTACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCat                                                                   <  1:3120935/93‑1 (MQ=255)
                      aTACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCat                                                                   >  2:7400580/1‑93 (MQ=255)
                      aTACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCat                                                                   >  1:3643484/1‑93 (MQ=255)
                       tACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCatg                                                                  <  1:4821723/93‑1 (MQ=255)
                        aCTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCatga                                                                 <  1:5751176/93‑1 (MQ=255)
                          tGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCatgatg                                                               >  2:1888762/1‑93 (MQ=255)
                           gCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGc                                                              <  1:2630603/93‑1 (MQ=255)
                             gTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCg                                                            >  1:6393626/1‑93 (MQ=255)
                               cAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAg                                                          <  1:2476450/93‑1 (MQ=255)
                                aGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGc                                                         <  1:599880/93‑1 (MQ=255)
                                 ggTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCt                                                        >  1:6822146/1‑93 (MQ=255)
                                  gTCATTACTACCAATACTAATGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTg                                                       >  1:678006/1‑93 (MQ=255)
                                  gTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTg                                                       >  1:4173710/1‑93 (MQ=255)
                                  gTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTg                                                       >  2:5455668/1‑93 (MQ=255)
                                   tCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTgg                                                      <  2:3587122/93‑1 (MQ=255)
                                    cATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGa                                                     >  2:5634438/1‑93 (MQ=255)
                                    cATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGa                                                     >  2:31153/1‑93 (MQ=255)
                                     aTTACTACCAATACTAAAGAAATCAATCTCTTCGGAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAt                                                    >  2:4958335/1‑93 (MQ=255)
                                     aTTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAt                                                    >  2:658527/1‑93 (MQ=255)
                                      ttACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAtt                                                   >  2:5338750/1‑93 (MQ=255)
                                      ttACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAtt                                                   >  2:631745/1‑93 (MQ=255)
                                      ttACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAtt                                                   >  2:1070933/1‑93 (MQ=255)
                                       tactacCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAttt                                                  >  1:7358856/1‑93 (MQ=255)
                                         ctacCAATCCTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTtc                                                <  2:1927708/93‑1 (MQ=255)
                                         ctacCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTtc                                                <  1:3339538/93‑1 (MQ=255)
                                          tacCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTtct                                               >  1:2064738/1‑93 (MQ=255)
                                          tacCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTtct                                               >  1:1403276/1‑93 (MQ=255)
                                            ccAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtca                                             <  2:7865712/93‑1 (MQ=255)
                                            ccAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtca                                             >  1:845383/1‑93 (MQ=255)
                                               aTACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtcatca                                          >  1:3463084/1‑93 (MQ=255)
                                               aTACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtcatca                                          <  2:4393385/93‑1 (MQ=255)
                                                 aCTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaaa                                        <  2:5162170/93‑1 (MQ=255)
                                                   tAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAGACg                                      >  2:23855/1‑93 (MQ=255)
                                                   tAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACg                                      >  1:803445/1‑93 (MQ=255)
                                                   tAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACg                                      >  2:2218209/1‑93 (MQ=255)
                                                   tAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACg                                      >  1:1231561/1‑93 (MQ=255)
                                                     aaGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGa                                    >  1:1034859/1‑93 (MQ=255)
                                                       gAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAt                                  >  2:6027982/1‑93 (MQ=255)
                                                        aaaTCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAtg                                 >  1:4274020/1‑93 (MQ=255)
                                                        aaaTCAATCTATTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAtg                                 <  1:4529961/93‑1 (MQ=255)
                                                         aatcaatcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAtgt                                >  1:6051661/1‑93 (MQ=255)
                                                          atcaatcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAtgtg                               >  1:2309694/1‑93 (MQ=255)
                                                          atcaatcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAtgtg                               >  2:2596523/1‑93 (MQ=255)
                                                           tcaatcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATgtgt                              <  2:3688738/93‑1 (MQ=255)
                                                             aatcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTc                            <  1:4006681/93‑1 (MQ=255)
                                                              atcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg                           >  1:1621973/1‑93 (MQ=255)
                                                              atcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg                           >  2:4825976/1‑93 (MQ=255)
                                                              atcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg                           <  2:1402626/93‑1 (MQ=255)
                                                              atcTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCg                           >  1:3013184/1‑93 (MQ=255)
                                                               tctcTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGt                          <  2:7054608/93‑1 (MQ=255)
                                                               tctcTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGt                          >  1:5119921/1‑93 (MQ=255)
                                                                  ctTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTAc                       <  1:7123054/93‑1 (MQ=255)
                                                                   ttCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACg                      >  2:331965/1‑93 (MQ=255)
                                                                    tCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGt                     <  1:3246112/93‑1 (MQ=255)
                                                                    tCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGt                     <  2:102846/93‑1 (MQ=255)
                                                                     cGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTa                    <  1:7218748/93‑1 (MQ=255)
                                                                     cGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTa                    <  2:3980213/93‑1 (MQ=255)
                                                                     cGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTa                    <  1:615170/93‑1 (MQ=255)
                                                                      gcagcaTTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAg                   >  2:126513/1‑93 (MQ=255)
                                                                      gcagcaTTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAg                   >  2:2740/1‑93 (MQ=255)
                                                                       cagcaTTGATCGATGATGAGCATCACCGACGTCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGt                  <  1:3569428/93‑1 (MQ=255)
                                                                       cagcaTTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGt                  <  2:6199829/93‑1 (MQ=255)
                                                                       cagcaTTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGt                  <  2:6856419/93‑1 (MQ=255)
                                                                        agcaTTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGtt                 >  2:7344312/1‑93 (MQ=255)
                                                                         gcaTTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTg                >  1:2158440/1‑93 (MQ=255)
                                                                         gcaTTGATCGATGATGAGCATCACCGACAGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAAAGGAATGTGTTCGGTACGTattcg                >  2:5718161/1‑89 (MQ=255)
                                                                            ttGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTg             <  2:526895/93‑1 (MQ=255)
                                                                            ttGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTg             <  1:6676638/93‑1 (MQ=255)
                                                                               aTCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGttt          <  1:379374/93‑1 (MQ=255)
                                                                               aTCGATAATGAGCATAACCGACGGCACATCCAGCATGATGACGAGCTGGAATTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGttt          <  2:7003176/93‑1 (MQ=255)
                                                                                tCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTg         >  1:6557394/1‑93 (MQ=255)
                                                                                  gatgatGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGc       <  1:5947803/93‑1 (MQ=255)
                                                                                   atgatgAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCt      <  1:2706108/93‑1 (MQ=255)
                                                                                   atgatgAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCt      >  1:2858281/1‑93 (MQ=255)
                                                                                     gatgaGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTc    >  1:3893478/1‑93 (MQ=255)
                                                                                      atgaGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTcc   <  2:6502704/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCG  >  NC_000913/4138067‑4138246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 226 total aligned reads displayed.
CGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAG  >  NC_000913/4138064‑4138250
                                                                                             |                                                                                             
CGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAG                                                                                               <  SRR4302543.2411856/94‑1 (MQ=60)
CGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAG                                                                                               >  SRR4302543.141346/1‑94 (MQ=60)
 GTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGC                                                                                              <  SRR4302543.7981489/94‑1 (MQ=60)
  TTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACA                                                                                             >  SRR4302543.3445697/1‑94 (MQ=60)
   TATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCAT                                                                                            <  SRR4302543.3647563/94‑1 (MQ=60)
    ATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATC                                                                                           >  SRR4302543.4482716/1‑94 (MQ=60)
      CGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCAC                                                                                         >  SRR4302543.2624809/1‑94 (MQ=60)
      CGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCAC                                                                                         >  SRR4302543.3712187/1‑94 (MQ=60)
        CGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCG                                                                                       >  SRR4302543.7776626/1‑94 (MQ=60)
        CGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCG                                                                                       >  SRR4302543.7454012/1‑94 (MQ=60)
        CGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCG                                                                                       >  SRR4302543.7819910/1‑94 (MQ=60)
        CGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCG                                                                                       >  SRR4302543.2451575/1‑94 (MQ=60)
         GATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGA                                                                                      >  SRR4302543.361933/1‑94 (MQ=60)
          ATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGAC                                                                                     <  SRR4302543.5816310/94‑1 (MQ=60)
          ATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGAC                                                                                     <  SRR4302543.7937075/94‑1 (MQ=60)
          ATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGAC                                                                                     >  SRR4302543.5716296/1‑94 (MQ=60)
          ATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGAC                                                                                     >  SRR4302543.772868/1‑94 (MQ=60)
           TCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACG                                                                                    >  SRR4302543.4604969/1‑94 (MQ=60)
           TCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATGGATCGATGATGAGCATCACCGACG                                                                                    <  SRR4302543.6084585/94‑1 (MQ=60)
            CCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGG                                                                                   >  SRR4302543.1377678/1‑94 (MQ=60)
             CACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGC                                                                                  >  SRR4302543.7426849/1‑94 (MQ=60)
             CACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGC                                                                                  >  SRR4302543.1491352/1‑94 (MQ=60)
             CACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGC                                                                                  >  SRR4302543.5001164/1‑94 (MQ=60)
             CACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACCGC                                                                                  >  SRR4302543.3994147/1‑94 (MQ=60)
              CCCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCA                                                                                 <  SRR4302543.2979354/94‑1 (MQ=60)
              ACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCA                                                                                 <  SRR4302543.7621318/94‑1 (MQ=60)
              ACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCA                                                                                 >  SRR4302543.4351661/1‑94 (MQ=60)
                CGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACT                                                                               >  SRR4302543.2067914/1‑94 (MQ=60)
                CGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACT                                                                               >  SRR4302543.1067790/1‑94 (MQ=60)
                 GCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTT                                                                              >  SRR4302543.1465159/1‑94 (MQ=60)
                  CCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCAGTTC                                                                             >  SRR4302543.29640/1‑94 (MQ=60)
                  CCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTC                                                                             >  SRR4302543.1677806/1‑94 (MQ=60)
                   CAGCAGATACTTCCTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCC                                                                            <  SRR4302543.6162016/94‑1 (MQ=60)
                    AGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCA                                                                           >  SRR4302543.5658766/1‑94 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAG                                                                          <  SRR4302543.3626368/94‑1 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAG                                                                          >  SRR4302543.3446518/1‑94 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAG                                                                          >  SRR4302543.7196516/1‑94 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAG                                                                          <  SRR4302543.5908506/94‑1 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAG                                                                          <  SRR4302543.4997800/94‑1 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAC                                                                          >  SRR4302543.1371884/1‑94 (MQ=60)
                     GCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGCCACTTCCAG                                                                          >  SRR4302543.821907/1‑94 (MQ=60)
                      CAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGC                                                                         >  SRR4302543.5125700/1‑94 (MQ=60)
                      CAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGC                                                                         <  SRR4302543.4698101/94‑1 (MQ=60)
                      CAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGC                                                                         <  SRR4302543.7884853/94‑1 (MQ=60)
                      CAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGC                                                                         <  SRR4302543.7669324/94‑1 (MQ=60)
                       AGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCA                                                                        >  SRR4302543.6135640/1‑94 (MQ=60)
                       AGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCA                                                                        >  SRR4302543.3694077/1‑94 (MQ=60)
                       AGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCA                                                                        >  SRR4302543.6918531/1‑94 (MQ=60)
                        GATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCAT                                                                       <  SRR4302543.604352/94‑1 (MQ=60)
                        GATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCAT                                                                       >  SRR4302543.3675526/1‑94 (MQ=60)
                        GATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCAT                                                                       <  SRR4302543.4942625/94‑1 (MQ=60)
                         ATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATG                                                                      <  SRR4302543.280968/94‑1 (MQ=60)
                         ATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATG                                                                      <  SRR4302543.3149005/94‑1 (MQ=60)
                          TACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGA                                                                     <  SRR4302543.4397968/94‑1 (MQ=60)
                           ACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGAT                                                                    <  SRR4302543.1591792/94‑1 (MQ=60)
                           ACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGAT                                                                    <  SRR4302543.5675318/94‑1 (MQ=60)
                            CTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATG                                                                   >  SRR4302543.4583965/1‑94 (MQ=60)
                            CTGCGTCAGGTCATGACTACCAATACTAAAGAAATCAATCTCGTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATG                                                                   <  SRR4302543.6859427/94‑1 (MQ=60)
                              TCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCCTCTCGCCGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCC                                                                 <  SRR4302543.4581519/94‑1 (MQ=60)
                              GCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCC                                                                 <  SRR4302543.7287402/94‑1 (MQ=60)
                              GCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCC                                                                 <  SRR4302543.3945597/94‑1 (MQ=60)
                              GCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCC                                                                 <  SRR4302543.2654414/94‑1 (MQ=60)
                              GCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCC                                                                 <  SRR4302543.7275703/94‑1 (MQ=60)
                               CGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCG                                                                <  SRR4302543.1063982/94‑1 (MQ=60)
                               CGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCG                                                                >  SRR4302543.6453812/1‑94 (MQ=60)
                               CGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCG                                                                >  SRR4302543.3685555/1‑94 (MQ=60)
                               CGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTGCGCAGCATTGAGCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCG                                                                <  SRR4302543.1063981/94‑1 (MQ=60)
                                 TCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAG                                                              <  SRR4302543.785087/94‑1 (MQ=60)
                                  CAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGC                                                             <  SRR4302543.3115601/94‑1 (MQ=60)
                                  CAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGC                                                             <  SRR4302543.2498844/94‑1 (MQ=60)
                                   AGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCT                                                            <  SRR4302543.4917792/94‑1 (MQ=60)
                                   AGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCT                                                            <  SRR4302543.605374/94‑1 (MQ=60)
                                   AGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCT                                                            >  SRR4302543.6886551/1‑94 (MQ=60)
                                    GGTCATTACTACCAATACTAATGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTG                                                           >  SRR4302543.684243/1‑94 (MQ=60)
                                    GGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGCGCTG                                                           >  SRR4302543.4211509/1‑94 (MQ=60)
                                    GGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTG                                                           >  SRR4302543.684245/1‑94 (MQ=60)
                                    GGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTG                                                           >  SRR4302543.4211508/1‑94 (MQ=60)
                                    GGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTG                                                           <  SRR4302543.7007975/94‑1 (MQ=60)
                                     GTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGG                                                          >  SRR4302543.139458/1‑94 (MQ=60)
                                     GTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGG                                                          >  SRR4302543.876303/1‑94 (MQ=60)
                                     GTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGG                                                          <  SRR4302543.3873308/94‑1 (MQ=60)
                                     GTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGG                                                          <  SRR4302543.6497279/94‑1 (MQ=60)
                                      TCATTACGACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGA                                                         <  SRR4302543.2248550/94‑1 (MQ=60)
                                       CATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAT                                                        <  SRR4302543.6293592/94‑1 (MQ=60)
                                        ATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATT                                                       <  SRR4302543.6715744/94‑1 (MQ=60)
                                        ATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATT                                                       >  SRR4302543.6089906/1‑94 (MQ=60)
                                        ATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATT                                                       >  SRR4302543.3182020/1‑94 (MQ=60)
                                         TTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTT                                                      >  SRR4302543.7429634/1‑94 (MQ=60)
                                          TACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTT                                                     <  SRR4302543.4212498/94‑1 (MQ=60)
                                           TCTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTC                                                    <  SRR4302543.2720905/94‑1 (MQ=60)
                                           ACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTC                                                    <  SRR4302543.6518429/94‑1 (MQ=60)
                                           ACTACAAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTC                                                    <  SRR4302543.2979917/94‑1 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.1415941/1‑94 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.6121446/1‑94 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.3246457/1‑94 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.2083202/1‑94 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   <  SRR4302543.3369622/94‑1 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.7549155/1‑94 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.1120274/1‑94 (MQ=60)
                                            CTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCT                                                   >  SRR4302543.2170949/1‑94 (MQ=60)
                                             TACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTC                                                  >  SRR4302543.814048/1‑94 (MQ=60)
                                              ACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCA                                                 >  SRR4302543.852879/1‑94 (MQ=60)
                                               CCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCAT                                                <  SRR4302543.3675654/94‑1 (MQ=60)
                                               CCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCAT                                                <  SRR4302543.7928369/94‑1 (MQ=60)
                                                CAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGCGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATC                                               >  SRR4302543.3656632/1‑94 (MQ=60)
                                                CAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATC                                               >  SRR4302543.3798160/1‑94 (MQ=60)
                                                 AATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCA                                              >  SRR4302543.3494103/1‑94 (MQ=60)
                                                 AATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCA                                              >  SRR4302543.2755745/1‑94 (MQ=60)
                                                 AATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATAA                                              >  SRR4302543.2449268/1‑94 (MQ=60)
                                                  ATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAA                                             <  SRR4302543.6677003/94‑1 (MQ=60)
                                                  ATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAA                                             <  SRR4302543.4820435/94‑1 (MQ=60)
                                                  ATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAA                                             >  SRR4302543.7298158/1‑94 (MQ=60)
                                                   TACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGCCGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAA                                            >  SRR4302543.4145454/1‑94 (MQ=60)
                                                   TACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAA                                            <  SRR4302543.6887536/94‑1 (MQ=60)
                                                     CTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACG                                          >  SRR4302543.810513/1‑94 (MQ=60)
                                                     CTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACG                                          >  SRR4302543.1100465/1‑94 (MQ=60)
                                                     CTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACG                                          >  SRR4302543.2801944/1‑94 (MQ=60)
                                                     CTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACG                                          >  SRR4302543.3552848/1‑94 (MQ=60)
                                                     CTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACG                                          <  SRR4302543.3038171/94‑1 (MQ=60)
                                                     CTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACG                                          >  SRR4302543.5768827/1‑94 (MQ=60)
                                                      TAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGG                                         <  SRR4302543.6039477/94‑1 (MQ=60)
                                                       AAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGA                                        >  SRR4302543.1044047/1‑94 (MQ=60)
                                                       AAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGA                                        >  SRR4302543.1553891/1‑94 (MQ=60)
                                                        AAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAA                                       >  SRR4302543.7793503/1‑94 (MQ=60)
                                                        AAGAAATCAATCTCTTCGCAGCATTGATCGACGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAA                                       >  SRR4302543.5455721/1‑94 (MQ=60)
                                                         AGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAT                                      >  SRR4302543.4207750/1‑94 (MQ=60)
                                                         AGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAT                                      <  SRR4302543.8520/94‑1 (MQ=60)
                                                         AGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAT                                      >  SRR4302543.3623865/1‑94 (MQ=60)
                                                         AGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAT                                      >  SRR4302543.3859728/1‑94 (MQ=60)
                                                         AGAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAT                                      >  SRR4302543.3623864/1‑94 (MQ=60)
                                                          GAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATG                                     <  SRR4302543.7455590/94‑1 (MQ=60)
                                                          GAAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATG                                     >  SRR4302543.4312863/1‑94 (MQ=60)
                                                           AAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGT                                    <  SRR4302543.6028947/94‑1 (MQ=60)
                                                           AAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGT                                    <  SRR4302543.7334533/94‑1 (MQ=60)
                                                           AAATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGT                                    >  SRR4302543.6108947/1‑94 (MQ=60)
                                                            AATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTG                                   >  SRR4302543.2330717/1‑94 (MQ=60)
                                                            AATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTG                                   >  SRR4302543.2903561/1‑94 (MQ=60)
                                                             ATCAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGT                                  >  SRR4302543.4702744/1‑94 (MQ=60)
                                                               CAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTC                                >  SRR4302543.4348021/1‑94 (MQ=60)
                                                               CAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTC                                >  SRR4302543.3230211/1‑94 (MQ=60)
                                                               CAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTC                                >  SRR4302543.4196179/1‑94 (MQ=60)
                                                               CAATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTC                                <  SRR4302543.257636/94‑1 (MQ=60)
                                                                AATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCG                               >  SRR4302543.3040458/1‑94 (MQ=60)
                                                                AATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCG                               >  SRR4302543.3482772/1‑94 (MQ=60)
                                                                AATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCG                               <  SRR4302543.6771701/94‑1 (MQ=60)
                                                                AATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCG                               >  SRR4302543.1636428/1‑94 (MQ=60)
                                                                 ATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGT                              <  SRR4302543.299385/94‑1 (MQ=60)
                                                                 ATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGT                              >  SRR4302543.644482/1‑94 (MQ=60)
                                                                 ATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGT                              <  SRR4302543.1666412/94‑1 (MQ=60)
                                                                 ATCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGT                              >  SRR4302543.5166219/1‑94 (MQ=60)
                                                                  TCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTT                             <  SRR4302543.5403007/94‑1 (MQ=60)
                                                                  TCTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTT                             >  SRR4302543.7631562/1‑94 (MQ=60)
                                                                   CTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGACTGTGTTCGTTA                            >  SRR4302543.3849984/1‑94 (MQ=60)
                                                                   CTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTA                            >  SRR4302543.4754630/1‑94 (MQ=60)
                                                                   CTCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTA                            >  SRR4302543.3343189/1‑94 (MQ=60)
                                                                    TCTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGCGCTGGATTTTCTCATCAAACGGACTGTGTTCGTTAC                           >  SRR4302543.2119458/1‑94 (MQ=60)
                                                                     CTTCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACG                          <  SRR4302543.7192250/94‑1 (MQ=60)
                                                                       TCGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTA                        <  SRR4302543.3275465/94‑1 (MQ=60)
                                                                        CGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAG                       >  SRR4302543.2682632/1‑94 (MQ=60)
                                                                        CGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAG                       <  SRR4302543.620795/94‑1 (MQ=60)
                                                                        CGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAG                       >  SRR4302543.4521193/1‑94 (MQ=60)
                                                                        CGCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAG                       >  SRR4302543.4521192/1‑94 (MQ=60)
                                                                         GCAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGT                      >  SRR4302543.3562044/1‑94 (MQ=60)
                                                                          CAGCATTGATCGATGATGAGCATCACCGACGTCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTT                     <  SRR4302543.3600990/94‑1 (MQ=60)
                                                                          CAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTT                     <  SRR4302543.5575612/94‑1 (MQ=60)
                                                                          CAGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTT                     <  SRR4302543.5987764/94‑1 (MQ=60)
                                                                           AGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTG                    >  SRR4302543.7965520/1‑94 (MQ=60)
                                                                           AGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTG                    >  SRR4302543.2177770/1‑94 (MQ=60)
                                                                           AGCATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTG                    <  SRR4302543.3300783/94‑1 (MQ=60)
                                                                              ATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTG                 >  SRR4302543.3802082/1‑94 (MQ=60)
                                                                              ATTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTG                 >  SRR4302543.4925621/1‑94 (MQ=60)
                                                                               TTGATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGT                <  SRR4302543.6739670/94‑1 (MQ=60)
                                                                                 GATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTT              <  SRR4302543.1448305/94‑1 (MQ=60)
                                                                                  ATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTG             >  SRR4302543.6619094/1‑94 (MQ=60)
                                                                                  ATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTG             <  SRR4302543.5128214/94‑1 (MQ=60)
                                                                                  ATCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTG             <  SRR4302543.382870/94‑1 (MQ=60)
                                                                                   TCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGG            <  SRR4302543.7921405/94‑1 (MQ=60)
                                                                                   TCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGG            <  SRR4302543.6757358/94‑1 (MQ=60)
                                                                                   TCGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGG            <  SRR4302543.6843271/94‑1 (MQ=60)
                                                                                    CGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGACTGAGTTCGTTACGTAGTTGCTGTTTGGC           >  SRR4302543.2224127/1‑94 (MQ=60)
                                                                                    CGATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGAATTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTATGGC           >  SRR4302543.3942780/1‑94 (MQ=60)
                                                                                     GATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCT          <  SRR4302543.1237398/94‑1 (MQ=60)
                                                                                     GATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCT          <  SRR4302543.30701/94‑1 (MQ=60)
                                                                                     GATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCT          <  SRR4302543.6002237/94‑1 (MQ=60)
                                                                                     GATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCT          >  SRR4302543.2884350/1‑94 (MQ=60)
                                                                                     GATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCT          <  SRR4302543.4508823/94‑1 (MQ=60)
                                                                                      ATGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTT         <  SRR4302543.2730664/94‑1 (MQ=60)
                                                                                       TGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTC        >  SRR4302543.2563362/1‑94 (MQ=60)
                                                                                       TGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTC        >  SRR4302543.2233722/1‑94 (MQ=60)
                                                                                       TGATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTC        >  SRR4302543.3927854/1‑94 (MQ=60)
                                                                                        GATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCC       <  SRR4302543.883082/94‑1 (MQ=60)
                                                                                        GATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCC       <  SRR4302543.1535575/94‑1 (MQ=60)
                                                                                        GATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCC       >  SRR4302543.1046859/1‑94 (MQ=60)
                                                                                         ATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCG      <  SRR4302543.7958964/94‑1 (MQ=60)
                                                                                         ATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCG      <  SRR4302543.648185/94‑1 (MQ=60)
                                                                                         ATGAGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCG      <  SRR4302543.3317182/94‑1 (MQ=60)
                                                                                            AGCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCA   <  SRR4302543.3723812/94‑1 (MQ=60)
                                                                                             GCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAG  <  SRR4302543.7917810/94‑1 (MQ=60)
                                                                                             GCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAG  >  SRR4302543.4733395/1‑94 (MQ=60)
                                                                                             GCATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAG  <  SRR4302543.4223297/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAG  >  NC_000913/4138064‑4138250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑