Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,140,312 G→A M68I (ATG→ATA)  frwC → PTS system fructose‑like enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,140,3120GA99.7% 1276.1 / ‑6.0 352M68I (ATG→ATA) frwCPTS system fructose‑like enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (187/164);  total (187/165)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 365 total aligned reads displayed.

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401
                                                                                          |                                                                                         
gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                         <  2:4457356/93‑1 (MQ=255)
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTg                                                                                        >  1:7334477/1‑93 (MQ=255)
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTg                                                                                        >  2:706032/1‑93 (MQ=255)
 ttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTCACGCTGATAGTg                                                                                        >  2:6972752/1‑93 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  2:6748729/93‑1 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  1:5692764/93‑1 (MQ=255)
    tATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCt                                                                                     >  1:114843/1‑93 (MQ=255)
      tGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCttt                                                                                   >  2:6428650/1‑93 (MQ=255)
      tGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCttt                                                                                   >  1:7756152/1‑93 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  2:7376946/93‑1 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                >  1:460053/1‑93 (MQ=255)
          aaaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTc                                                                               >  2:3903837/1‑93 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              >  2:6024310/1‑93 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            <  1:321993/93‑1 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCAGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            <  1:4833991/93‑1 (MQ=255)
                 cAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGt                                                                        <  1:835897/93‑1 (MQ=255)
                  aGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTa                                                                       <  2:1378582/93‑1 (MQ=255)
                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                      >  2:2205138/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                     >  2:1138606/1‑93 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATc                                                                   <  2:5247141/93‑1 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATc                                                                   <  2:3706672/93‑1 (MQ=255)
                       cGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCg                                                                  <  1:6980150/93‑1 (MQ=255)
                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 >  1:2089939/1‑93 (MQ=255)
                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 <  1:1912214/93‑1 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAATCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                <  2:1731540/93‑1 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCGGCGTACATCGGt                                                                >  2:3116/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  1:4795455/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  1:1194323/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  2:2627280/1‑93 (MQ=255)
                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                              <  2:183722/93‑1 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATGGGCGTTGCGGGCTTGGCGCTGATAGTGCCTTTCCTCGCACCCCACATCGGTTATTcg                                                          >  1:4968852/1‑92 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTcc                                                          >  2:665285/1‑93 (MQ=255)
                               gTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTcc                                                          >  1:5908634/1‑93 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                         >  2:5507890/1‑93 (MQ=255)
                                tAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCa                                                         <  1:6796779/93‑1 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  1:6640224/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       <  2:7550296/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTCTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  2:6279807/1‑93 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     >  1:3605327/1‑93 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCa                                                    <  2:2886458/93‑1 (MQ=255)
                                     gATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCa                                                    >  2:3300005/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  2:2731019/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:5037896/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:2526647/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCCGTTATTCCATTGCag                                                   >  1:3512236/1‑93 (MQ=255)
                                        ccAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCagag                                                 <  1:6326547/93‑1 (MQ=255)
                                        ccAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCagag                                                 >  2:2546407/1‑93 (MQ=255)
                                         cAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGc                                                >  1:4193192/1‑93 (MQ=255)
                                         cAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGc                                                <  2:2987135/93‑1 (MQ=255)
                                           aaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGt                                              <  2:4818616/93‑1 (MQ=255)
                                           aaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGt                                              <  1:5136088/93‑1 (MQ=255)
                                            aTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGtt                                             <  2:6319366/93‑1 (MQ=255)
                                            aTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGtt                                             >  2:3207855/1‑93 (MQ=255)
                                             tCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTc                                            >  1:4625136/1‑93 (MQ=255)
                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           >  2:7710909/1‑93 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  2:657941/1‑93 (MQ=255)
                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         <  2:6255432/93‑1 (MQ=255)
                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        <  2:3798530/93‑1 (MQ=255)
                                                    aaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTg                                     <  1:2930506/93‑1 (MQ=255)
                                                     aaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTgg                                    >  1:4020952/1‑93 (MQ=255)
                                                     aaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGGACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTgg                                    >  1:4397835/1‑93 (MQ=255)
                                                      aCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGc                                   >  1:6921213/1‑93 (MQ=255)
                                                       cTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCt                                  <  2:7932973/93‑1 (MQ=255)
                                                        tGTTTGATATCAGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGAAGAGCGTTCTGCGCTGGCTc                                 >  2:2312210/1‑93 (MQ=255)
                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               >  1:5620024/1‑93 (MQ=255)
                                                           ttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGt                              >  1:3794509/1‑93 (MQ=255)
                                                            tGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTg                             >  1:3348595/1‑93 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          >  1:1452419/1‑93 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  2:7254867/93‑1 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  1:5362790/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  2:1512664/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  2:3561688/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  2:5795540/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       >  1:4091111/1‑93 (MQ=255)
                                                                    gCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCg                     <  2:5668650/93‑1 (MQ=255)
                                                                    gCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCg                     >  2:4999432/1‑93 (MQ=255)
                                                                    gCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCGCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCg                     <  1:7641569/93‑1 (MQ=255)
                                                                      gTTGCGGGCTTGGCGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   <  1:5512475/93‑1 (MQ=255)
                                                                       ttGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTg                  >  1:2723652/1‑93 (MQ=255)
                                                                         gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGcc                >  1:7903095/1‑93 (MQ=255)
                                                                          cGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCt               <  1:3038200/93‑1 (MQ=255)
                                                                          cGGGCTTGACGCTGATAGTGCCATTCCTGGCAGCGTAAATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCTGGGCCt               <  2:422612/93‑1 (MQ=255)
                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             <  2:1202763/93‑1 (MQ=255)
                                                                              cTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGt           >  2:4630800/1‑93 (MQ=255)
                                                                              cTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGt           >  1:26490/1‑93 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          >  2:2116019/1‑93 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          <  1:101889/93‑1 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          >  1:6507796/1‑93 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          >  2:4073235/1‑93 (MQ=255)
                                                                                tGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTg         >  1:7486654/1‑93 (MQ=255)
                                                                                 gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTgg        <  2:5387700/93‑1 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  1:6049360/1‑93 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     <  2:6832934/93‑1 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  2:1240762/1‑93 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  2:3452578/1‑93 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGaaaa    >  2:4411201/1‑92 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    >  1:6242914/1‑93 (MQ=255)
                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   >  1:4497048/1‑93 (MQ=255)
                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   <  1:159573/93‑1 (MQ=255)
                                                                                       gATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAg  >  2:1584828/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  >  NC_000913/4140220‑4140404
                                                                                            |                                                                                            
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                             <  SRR4302543.1942205/94‑1 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                             <  SRR4302543.5098575/94‑1 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                             <  SRR4302543.1159397/94‑1 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                             <  SRR4302543.55451/94‑1 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                             >  SRR4302543.708731/1‑94 (MQ=60)
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                             >  SRR4302543.1314226/1‑94 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                            <  SRR4302543.5037157/94‑1 (MQ=60)
 TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                            >  SRR4302543.4573485/1‑94 (MQ=60)
  GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                           >  SRR4302543.7405047/1‑94 (MQ=60)
   TTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGC                                                                                          >  SRR4302543.7765402/1‑94 (MQ=60)
   TTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGC                                                                                          >  SRR4302543.4108798/1‑94 (MQ=60)
     GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        <  SRR4302543.1200553/94‑1 (MQ=60)
     GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        <  SRR4302543.5744710/94‑1 (MQ=60)
     GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                        >  SRR4302543.115884/1‑94 (MQ=60)
       ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                      >  SRR4302543.7829365/1‑94 (MQ=60)
       ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                      <  SRR4302543.4150346/94‑1 (MQ=60)
       ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                      >  SRR4302543.393747/1‑94 (MQ=60)
         GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                    <  SRR4302543.4673352/94‑1 (MQ=60)
         GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                    >  SRR4302543.5472798/1‑94 (MQ=60)
         GGCAAAGGCGCAGTGCAGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                    <  SRR4302543.1201840/94‑1 (MQ=60)
          GCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCT                                                                                   >  SRR4302543.464330/1‑94 (MQ=60)
           CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                  <  SRR4302543.4451521/94‑1 (MQ=60)
            AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGTTAGTGCCTTTCCTCC                                                                                 >  SRR4302543.7966636/1‑94 (MQ=60)
            AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                                 >  SRR4302543.104977/1‑94 (MQ=60)
             AAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGC                                                                                <  SRR4302543.2240719/94‑1 (MQ=60)
              AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                               <  SRR4302543.495310/94‑1 (MQ=60)
              AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                               >  SRR4302543.6386027/1‑94 (MQ=60)
               GGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAG                                                                              <  SRR4302543.359774/94‑1 (MQ=60)
               GGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAG                                                                              <  SRR4302543.324909/94‑1 (MQ=60)
               GGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCAGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAG                                                                              <  SRR4302543.4878082/94‑1 (MQ=60)
                GCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGC                                                                             <  SRR4302543.60427/94‑1 (MQ=60)
                  GCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGT                                                                           >  SRR4302543.7974021/1‑94 (MQ=60)
                   CAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTA                                                                          <  SRR4302543.843277/94‑1 (MQ=60)
                    AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                         <  SRR4302543.6621399/94‑1 (MQ=60)
                    AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                         <  SRR4302543.2885859/94‑1 (MQ=60)
                     GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                        <  SRR4302543.5969188/94‑1 (MQ=60)
                     GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                        <  SRR4302543.976032/94‑1 (MQ=60)
                      TGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAT                                                                       <  SRR4302543.5961190/94‑1 (MQ=60)
                      TGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAT                                                                       >  SRR4302543.5175093/1‑94 (MQ=60)
                       GCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATC                                                                      <  SRR4302543.3899541/94‑1 (MQ=60)
                        CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                     <  SRR4302543.6886322/94‑1 (MQ=60)
                        CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                     >  SRR4302543.7368747/1‑94 (MQ=60)
                         CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                    <  SRR4302543.7045841/94‑1 (MQ=60)
                         CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                    >  SRR4302543.2108612/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302543.5597814/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   <  SRR4302543.1929449/94‑1 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302543.1205092/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302543.4839288/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   >  SRR4302543.4839287/1‑94 (MQ=60)
                          GGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGT                                                                   <  SRR4302543.4383217/94‑1 (MQ=60)
                           GATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTT                                                                  <  SRR4302543.5815672/94‑1 (MQ=60)
                           GATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTT                                                                  <  SRR4302543.5147841/94‑1 (MQ=60)
                            ATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTA                                                                 <  SRR4302543.3504083/94‑1 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                <  SRR4302543.5714856/94‑1 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                <  SRR4302543.419044/94‑1 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                <  SRR4302543.6148656/94‑1 (MQ=60)
                             TGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAT                                                                <  SRR4302543.4992192/94‑1 (MQ=60)
                              GCGGTAGGCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCGGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATT                                                               <  SRR4302543.4675888/94‑1 (MQ=60)
                              GCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATT                                                               <  SRR4302543.4675889/94‑1 (MQ=60)
                              GCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATT                                                               <  SRR4302543.5844649/94‑1 (MQ=60)
                                cttagacgatctaaatctgataaatctgcttgataatggctttgagtGCTTGACGCTGATATTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                             <  SRR4302543.4555482/47‑1 (MQ=60)
                                CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATGGGCGTTGCGGGCTTGGCGCTGATAGTGCCTTTCCTCGCACCCCACATCGGTTATTCG                                                             >  SRR4302543.5014076/1‑94 (MQ=60)
                                CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                             <  SRR4302543.1590050/94‑1 (MQ=60)
                                CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                             >  SRR4302543.5962574/1‑94 (MQ=60)
                                CGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCC                                                             <  SRR4302543.5389646/94‑1 (MQ=60)
                                 GTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCA                                                            <  SRR4302543.1941650/94‑1 (MQ=60)
                                  TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                           <  SRR4302543.6860963/94‑1 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                          >  SRR4302543.5536984/1‑94 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                          >  SRR4302543.6371016/1‑94 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                          >  SRR4302543.6371017/1‑94 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                          <  SRR4302543.6702883/94‑1 (MQ=60)
                                   AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGAAGCGTACATCGGTTATTCCATT                                                          >  SRR4302543.5536985/1‑94 (MQ=60)
                                    GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                         <  SRR4302543.7352627/94‑1 (MQ=60)
                                     CCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGC                                                        >  SRR4302543.3637141/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      >  SRR4302543.5021889/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      >  SRR4302543.2549629/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      >  SRR4302543.5083718/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      <  SRR4302543.3123178/94‑1 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                      >  SRR4302543.4094528/1‑94 (MQ=60)
                                       GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCCGTTATTCCATTGCAG                                                      >  SRR4302543.3543614/1‑94 (MQ=60)
                                         TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                    >  SRR4302543.1232122/1‑94 (MQ=60)
                                          CCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                   >  SRR4302543.4231213/1‑94 (MQ=60)
                                          CCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                   <  SRR4302543.6386047/94‑1 (MQ=60)
                                             AATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTT                                                <  SRR4302543.5182517/94‑1 (MQ=60)
                                             AATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTT                                                >  SRR4302543.166934/1‑94 (MQ=60)
                                              ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                               >  SRR4302543.4667157/1‑94 (MQ=60)
                                              ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                               >  SRR4302543.487836/1‑94 (MQ=60)
                                               TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                              <  SRR4302543.5618619/94‑1 (MQ=60)
                                               TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                              <  SRR4302543.3991492/94‑1 (MQ=60)
                                               ACTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                              <  SRR4302543.1169415/94‑1 (MQ=60)
                                                CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             >  SRR4302543.7663404/1‑94 (MQ=60)
                                                CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             >  SRR4302543.3806185/1‑94 (MQ=60)
                                                CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                             >  SRR4302543.1054237/1‑94 (MQ=60)
                                                 TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                            >  SRR4302543.1701650/1‑94 (MQ=60)
                                                 TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                            >  SRR4302543.3177823/1‑94 (MQ=60)
                                                 TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                            <  SRR4302543.1319006/94‑1 (MQ=60)
                                                  GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                           <  SRR4302543.2704469/94‑1 (MQ=60)
                                                  GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                           <  SRR4302543.3487203/94‑1 (MQ=60)
                                                  GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                           <  SRR4302543.7023962/94‑1 (MQ=60)
                                                  GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                           >  SRR4302543.6398015/1‑94 (MQ=60)
                                                    AAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCACGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCT                                         <  SRR4302543.7987625/94‑1 (MQ=60)
                                                     AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                        >  SRR4302543.3702904/1‑94 (MQ=60)
                                                     AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                        >  SRR4302543.2099410/1‑94 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                       <  SRR4302543.2957121/94‑1 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                       >  SRR4302543.4257775/1‑94 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                       >  SRR4302543.1869953/1‑94 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                       >  SRR4302543.4056867/1‑94 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                       <  SRR4302543.3444122/94‑1 (MQ=60)
                                                      AAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGGACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGG                                       >  SRR4302543.4437702/1‑94 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                      >  SRR4302543.6860835/1‑94 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                      >  SRR4302543.6986287/1‑94 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                      >  SRR4302543.3268952/1‑94 (MQ=60)
                                                       AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                      <  SRR4302543.3696317/94‑1 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                    >  SRR4302543.6549452/1‑94 (MQ=60)
                                                         CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                    <  SRR4302543.7645554/94‑1 (MQ=60)
                                                           GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                  >  SRR4302543.7180917/1‑94 (MQ=60)
                                                           GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                  <  SRR4302543.2558899/94‑1 (MQ=60)
                                                           GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                  >  SRR4302543.156984/1‑94 (MQ=60)
                                                           GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                  >  SRR4302543.5671239/1‑94 (MQ=60)
                                                           GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGAGTTCTGCGCTGGCTCCG                                  >  SRR4302543.6927692/1‑94 (MQ=60)
                                                            TTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGT                                 >  SRR4302543.3827939/1‑94 (MQ=60)
                                                             TTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTG                                >  SRR4302543.3378745/1‑94 (MQ=60)
                                                             TTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTG                                >  SRR4302543.6936525/1‑94 (MQ=60)
                                                              TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                               >  SRR4302543.746984/1‑94 (MQ=60)
                                                              TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                               >  SRR4302543.2504438/1‑94 (MQ=60)
                                                              TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                               >  SRR4302543.3314286/1‑94 (MQ=60)
                                                              TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                               >  SRR4302543.4845655/1‑94 (MQ=60)
                                                                ATATCGGCGTTGCGGGCTTGCCGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                             >  SRR4302543.5606385/1‑94 (MQ=60)
                                                                ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                             >  SRR4302543.2814856/1‑94 (MQ=60)
                                                                ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                             >  SRR4302543.1465532/1‑94 (MQ=60)
                                                                ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                             >  SRR4302543.4538560/1‑94 (MQ=60)
                                                                ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                             >  SRR4302543.2554824/1‑94 (MQ=60)
                                                                 TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                            <  SRR4302543.3009548/94‑1 (MQ=60)
                                                                   TCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAT                          <  SRR4302543.5411328/94‑1 (MQ=60)
                                                                   TCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAT                          >  SRR4302543.4127801/1‑94 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         <  SRR4302543.832316/94‑1 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         <  SRR4302543.6622200/94‑1 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         >  SRR4302543.218891/1‑94 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         <  SRR4302543.6214738/94‑1 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         >  SRR4302543.4586919/1‑94 (MQ=60)
                                                                    CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                         <  SRR4302543.832315/94‑1 (MQ=60)
                                                                     GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                        >  SRR4302543.5153584/1‑94 (MQ=60)
                                                                      GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCGCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                       <  SRR4302543.7714050/94‑1 (MQ=60)
                                                                       CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                      <  SRR4302543.6847598/94‑1 (MQ=60)
                                                                       CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                      <  SRR4302543.7726140/94‑1 (MQ=60)
                                                                       CGTTGCGGGCTTGACGCTGAGAGTGCCGTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                      <  SRR4302543.416324/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGGCGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     <  SRR4302543.5562532/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     >  SRR4302543.156735/1‑94 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     >  SRR4302543.2748424/1‑94 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     >  SRR4302543.7041190/1‑94 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     >  SRR4302543.5283468/1‑94 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                     <  SRR4302543.5048480/94‑1 (MQ=60)
                                                                        GTTGCGGGCTTGACGCTGATAGTCCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTC                     >  SRR4302543.2748423/1‑94 (MQ=60)
                                                                          TGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCC                   >  SRR4302543.7977372/1‑94 (MQ=60)
                                                                          TGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCC                   <  SRR4302543.5739781/94‑1 (MQ=60)
                                                                           GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                  <  SRR4302543.261939/94‑1 (MQ=60)
                                                                            CGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTG                 <  SRR4302543.3065612/94‑1 (MQ=60)
                                                                             GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                <  SRR4302543.5071881/94‑1 (MQ=60)
                                                                             GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                >  SRR4302543.4396899/1‑94 (MQ=60)
                                                                             GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG                >  SRR4302543.7578793/1‑94 (MQ=60)
                                                                               GCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGT              >  SRR4302543.26727/1‑94 (MQ=60)
                                                                                CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTT             >  SRR4302543.6569098/1‑94 (MQ=60)
                                                                                CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTT             >  SRR4302543.2966167/1‑94 (MQ=60)
                                                                                CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTG             >  SRR4302543.6737830/1‑94 (MQ=60)
                                                                                 TTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTG            >  SRR4302543.7558295/1‑94 (MQ=60)
                                                                                 TTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTG            <  SRR4302543.102830/94‑1 (MQ=60)
                                                                                 TTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTG            <  SRR4302543.4655199/94‑1 (MQ=60)
                                                                                   GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGT          >  SRR4302543.301907/1‑94 (MQ=60)
                                                                                    ACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGCTGGTA         >  SRR4302543.330798/1‑94 (MQ=60)
                                                                                     CGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAA        >  SRR4302543.6106622/1‑94 (MQ=60)
                                                                                      GCTGATGCTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAC       <  SRR4302543.7777280/94‑1 (MQ=60)
                                                                                      GCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAC       >  SRR4302543.6301808/1‑94 (MQ=60)
                                                                                      GCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGAGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTCGGGTGGTAAA       >  SRR4302543.7172504/1‑94 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      >  SRR4302543.7557492/1‑94 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      <  SRR4302543.639571/94‑1 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      <  SRR4302543.3634534/94‑1 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      >  SRR4302543.598185/1‑94 (MQ=60)
                                                                                       CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      >  SRR4302543.4537918/1‑94 (MQ=60)
                                                                                       CTGATAGTGCCTTGCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGTCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA      <  SRR4302543.865590/94‑1 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG     <  SRR4302543.160940/94‑1 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG     >  SRR4302543.4482434/1‑94 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG     >  SRR4302543.4654572/1‑94 (MQ=60)
                                                                                        TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG     <  SRR4302543.2895546/94‑1 (MQ=60)
                                                                                          ATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCT   >  SRR4302543.6734112/1‑94 (MQ=60)
                                                                                           TAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  <  SRR4302543.7711582/94‑1 (MQ=60)
                                                                                           TAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  >  SRR4302543.2122804/1‑94 (MQ=60)
                                                                                           TAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAGTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  <  SRR4302543.3149855/94‑1 (MQ=60)
                                                                                            |                                                                                            
ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGCTT  >  NC_000913/4140220‑4140404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑