Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,264,802	T→C	F180L (TTT→CTT)	yeiQ →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,802	0	T	C	99.3%	431.4 / NA	139	F180L (TTT→CTT)	yeiQ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (74/63); total (75/64)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891

cACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGctt                                                                                         >  2:782775/1‑93 (MQ=255)

 aCGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTAAACCGCTTa                                                                                        >  2:1501940/1‑93 (MQ=255)

   gATCTACAAACCCCTGCAGAACCTCACTCCGCACCGGGAATTCTCGTCGAAGCACTGAAACGCCGCCGTGACAGGGGCCTTACACCGCTTccc                                                                                      >  2:3400842/1‑93 (MQ=255)

    aTCTACAACCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCg                                                                                     <  2:150677/93‑1 (MQ=255)

     tCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGt                                                                                    >  2:3061964/1‑93 (MQ=255)

      cTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTg                                                                                   <  1:4587891/93‑1 (MQ=255)

       tACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGc                                                                                  >  2:1950423/1‑93 (MQ=255)

       tACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAAAGCCGCCGTGAGCGCGGCCTTAAACCGCTTAACGTGc                                                                                  >  2:414931/1‑93 (MQ=255)

          aaaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGctct                                                                               >  2:2750762/1‑93 (MQ=255)

          aaaCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGctct                                                                               >  1:2225368/1‑93 (MQ=255)

            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             <  2:2034209/93‑1 (MQ=255)

            aCCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTcc                                                                             >  2:4215880/1‑93 (MQ=255)

             ccccGGAAGACCCTCACTCCGCCCGGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCt                                                                            <  1:67518/93‑1 (MQ=255)

              cccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTg                                                                           >  1:2410386/1‑93 (MQ=255)

               ccTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGc                                                                          <  2:1846703/93‑1 (MQ=255)

                cTGAAGAACCTCACTCCGCACCGGGGATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCg                                                                         >  2:2349491/1‑93 (MQ=255)

                  gaagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAc                                                                       >  1:4600902/1‑93 (MQ=255)

                   aagaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAAAGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACa                                                                      >  2:3288154/1‑93 (MQ=255)

                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     <  1:813369/93‑1 (MQ=255)

                    agaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACaa                                                                     <  1:2796281/93‑1 (MQ=255)

                     gaaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAat                                                                    <  1:456871/93‑1 (MQ=255)

                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   <  1:1903005/93‑1 (MQ=255)

                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   >  1:1547296/1‑93 (MQ=255)

                      aaCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAata                                                                   <  1:3979106/93‑1 (MQ=255)

                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  >  2:1358909/1‑93 (MQ=255)

                       aCCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAatat                                                                  >  2:3873939/1‑93 (MQ=255)

                        ccTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAtt                                                                 <  1:1762735/93‑1 (MQ=255)

                         cTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTc                                                                >  1:1080088/1‑93 (MQ=255)

                         cTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTc                                                                <  2:1624159/93‑1 (MQ=255)

                          tCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTcc                                                               >  2:2162130/1‑93 (MQ=255)

                           cACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTccc                                                              <  1:1919698/93‑1 (MQ=255)

                           cACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTccc                                                              <  1:2491568/93‑1 (MQ=255)

                             cTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGACGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTACTGCGACAATATTctcaa                                                            >  2:584906/1‑89 (MQ=255)

                               ccGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACa                                                          <  2:4161089/93‑1 (MQ=255)

                                 gCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAt                                                        >  1:1554849/1‑93 (MQ=255)

                                 gCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAt                                                        >  2:2585560/1‑93 (MQ=255)

                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      >  1:3051099/1‑93 (MQ=255)

                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      >  1:587657/1‑93 (MQ=255)

                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      >  1:1775472/1‑93 (MQ=255)

                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      >  2:2899584/1‑93 (MQ=255)

                                   aCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATgg                                                      <  1:2942955/93‑1 (MQ=255)

                                    ccGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGt                                                     <  1:2903686/93‑1 (MQ=255)

                                    ccGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGt                                                     <  1:3180733/93‑1 (MQ=255)

                                     cGGTTATTCCCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTc                                                    <  1:1032028/93‑1 (MQ=255)

                                     cGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTc                                                    <  1:1171421/93‑1 (MQ=255)

                                     cGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTc                                                    <  2:3993972/93‑1 (MQ=255)

                                      gggTATTCTCGTCGAAGCACTGAACCGCCGCGGTGAGCGCTGCCTTACTCCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCa                                                   <  2:2066537/93‑1 (MQ=255)

                                       ggTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAt                                                  >  1:4126634/1‑93 (MQ=255)

                                        gTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGATTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtg                                                 >  2:675372/1‑93 (MQ=255)

                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               >  2:353563/1‑93 (MQ=255)

                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               <  1:2628817/93‑1 (MQ=255)

                                          aTTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAtgtg                                               <  1:3488507/93‑1 (MQ=255)

                                              tcgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGGCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTGATGTGGTGa                                           >  1:2198936/1‑93 (MQ=255)

                                              tcgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGa                                           >  2:1366307/1‑93 (MQ=255)

                                              tcgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGa                                           >  2:3279853/1‑93 (MQ=255)

                                              tcgtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGa                                           >  2:4183343/1‑93 (MQ=255)

                                                gtcgAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaa                                         >  1:3836021/1‑93 (MQ=255)

                                                  cgAAGCACCGCAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGaaaaa                                       <  2:4657642/93‑1 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGCCAATGGTCATGTGGTGAAAAAc                                      >  2:3633049/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  2:1923034/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  2:2576568/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:4163391/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      >  1:3177389/1‑93 (MQ=255)

                                                   gAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAc                                      <  1:2634835/93‑1 (MQ=255)

                                                    aaGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcg                                     <  1:4424341/93‑1 (MQ=255)

                                                     aGCACTGAACCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgc                                    <  1:4378842/93‑1 (MQ=255)

                                                     aGCACTGAAACGCCGCCGTGAGCGCGGCCTTACTCCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAcgc                                    <  2:4422848/93‑1 (MQ=255)

                                                       cACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCgg                                  <  2:2916512/93‑1 (MQ=255)

                                                       cACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCgg                                  <  1:2584013/93‑1 (MQ=255)

                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATCTTTCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 >  2:1831511/1‑93 (MQ=255)

                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 >  2:2962679/1‑93 (MQ=255)

                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 >  1:1327080/1‑93 (MQ=255)

                                                        aCTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGt                                 >  1:1231726/1‑93 (MQ=255)

                                                         cTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTg                                >  1:1010231/1‑93 (MQ=255)

                                                         cTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTg                                >  2:2268507/1‑93 (MQ=255)

                                                          tGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGc                               <  1:706474/93‑1 (MQ=255)

                                                          tGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGc                               >  1:4439022/1‑93 (MQ=255)

                                                          tGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGc                               >  2:3340365/1‑93 (MQ=255)

                                                             aaCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTgg                            <  2:4249605/93‑1 (MQ=255)

                                                                  cgccgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATg                       <  1:2038115/93‑1 (MQ=255)

                                                                     cgTGAGCGCGGCCTTACCCCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    <  1:3289256/93‑1 (MQ=255)

                                                                     cgTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCa                    <  1:3499863/93‑1 (MQ=255)

                                                                     cgTGAGCGCGGCCGTACACCGCTTACCGTGCTCTCCTGCTACAATATTCCCGACAATGGCCATGTGGTGAAAGACGCGGTGCTGGGAATggaa                    >  2:569122/1‑91 (MQ=255)

                                                                      gTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAg                   >  2:2429640/1‑93 (MQ=255)

                                                                         aGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGaaa                <  2:2071667/93‑1 (MQ=255)

                                                                          gcgcGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGaaaa               >  1:3069896/1‑93 (MQ=255)

                                                                           cgcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGaaaaa              >  1:539940/1‑93 (MQ=255)

                                                                            gcgGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAAc             <  1:2741463/93‑1 (MQ=255)

                                                                               gctttACCCCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          <  2:1400379/90‑1 (MQ=255)

                                                                               gCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGtt          >  1:113668/1‑93 (MQ=255)

                                                                                ccTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTc         >  1:803965/1‑93 (MQ=255)

                                                                                 cTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCg        >  1:1800554/1‑93 (MQ=255)

                                                                                 cTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCg        >  2:269061/1‑93 (MQ=255)

                                                                                  ttACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGc       >  1:847154/1‑93 (MQ=255)

                                                                                  ttACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGc       >  1:4306408/1‑93 (MQ=255)

                                                                                   tACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGcc      >  1:2082900/1‑93 (MQ=255)

                                                                                   tACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGGGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGcc      >  2:3389944/1‑93 (MQ=255)

                                                                                    acacCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCa     <  2:4387476/93‑1 (MQ=255)

                                                                                      acCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGa   >  2:4676400/1‑93 (MQ=255)

                                                                                       ccGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGaa  <  2:2393739/93‑1 (MQ=255)

CACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAA  >  NC_000913/2264712‑2264891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT  >  NC_000913/2264709‑2264893

ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGC                                                                                             >  SRR4302535.261536/1‑94 (MQ=60)

ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGC                                                                                             >  SRR4302535.2472985/1‑94 (MQ=60)

    ACGAGCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCCCTGAAACGCCGCCGTCAGCGCGGCCTTACCCCGCTTAC                                                                                         <  SRR4302535.3640019/94‑1 (MQ=60)

     CGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACC                                                                                        >  SRR4302535.4419337/1‑94 (MQ=60)

         CTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGC                                                                                    <  SRR4302535.4619130/94‑1 (MQ=60)

            CAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCT                                                                                 >  SRR4302535.338167/1‑94 (MQ=60)

            CAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCT                                                                                 >  SRR4302535.2241203/1‑94 (MQ=60)

                CCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTG                                                                             >  SRR4302535.2427737/1‑94 (MQ=60)

                CCCCGGAAGACCCTCACTCCGCCCGGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTG                                                                             <  SRR4302535.68021/94‑1 (MQ=60)

                  CCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCG                                                                           <  SRR4302535.1139872/94‑1 (MQ=60)

                  CCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCG                                                                           >  SRR4302535.802561/1‑94 (MQ=60)

                    TGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGAC                                                                         >  SRR4302535.4632192/1‑94 (MQ=60)

                     GAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACA                                                                        <  SRR4302535.3158474/94‑1 (MQ=60)

                      AAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAA                                                                       <  SRR4302535.2251883/94‑1 (MQ=60)

                      AAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAA                                                                       >  SRR4302535.1839462/1‑94 (MQ=60)

                       AGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                      <  SRR4302535.819071/94‑1 (MQ=60)

                       AGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAAT                                                                      <  SRR4302535.2816182/94‑1 (MQ=60)

                        GAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATA                                                                     >  SRR4302535.1558441/1‑94 (MQ=60)

                        GAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATA                                                                     <  SRR4302535.460064/94‑1 (MQ=60)

                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                    <  SRR4302535.1916700/94‑1 (MQ=60)

                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                    <  SRR4302535.4006295/94‑1 (MQ=60)

                         AACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATAT                                                                    <  SRR4302535.1645830/94‑1 (MQ=60)

                           CCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTC                                                                  >  SRR4302535.1087772/1‑94 (MQ=60)

                           CCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTC                                                                  <  SRR4302535.1775503/94‑1 (MQ=60)

                             TCTCTCCGCTCCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACCCCGCTTACCGTGCTCTCCTGCGACAATATTCCC                                                                <  SRR4302535.2285436/94‑1 (MQ=60)

                              CACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCG                                                               >  SRR4302535.4639852/1‑94 (MQ=60)

                              CACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCG                                                               <  SRR4302535.2509546/94‑1 (MQ=60)

                              CACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCG                                                               <  SRR4302535.1933536/94‑1 (MQ=60)

                                   CGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAT                                                          >  SRR4302535.1566055/1‑94 (MQ=60)

                                   CGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAAT                                                          >  SRR4302535.423630/1‑94 (MQ=60)

                                     CACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGG                                                        >  SRR4302535.1788330/1‑94 (MQ=60)

                                     CACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGG                                                        >  SRR4302535.591776/1‑94 (MQ=60)

                                     CACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGG                                                        >  SRR4302535.3072473/1‑94 (MQ=60)

                                      ACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGT                                                       <  SRR4302535.2963645/94‑1 (MQ=60)

                                       CCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTC                                                      <  SRR4302535.3202720/94‑1 (MQ=60)

                                       CCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTC                                                      <  SRR4302535.2924210/94‑1 (MQ=60)

                                       CCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTC                                                      <  SRR4302535.2835061/94‑1 (MQ=60)

                                        CGGTTATTCCCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCA                                                     <  SRR4302535.1039358/94‑1 (MQ=60)

                                        CGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCA                                                     <  SRR4302535.1179784/94‑1 (MQ=60)

                                        CGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCA                                                     >  SRR4302535.2551934/1‑94 (MQ=60)

                                         GGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCAT                                                    >  SRR4302535.4154872/1‑94 (MQ=60)

                                             ATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGG                                                <  SRR4302535.2647654/94‑1 (MQ=60)

                                             ATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGG                                                <  SRR4302535.3512727/94‑1 (MQ=60)

                                                CTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGGCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTGATGTGGTGA                                             >  SRR4302535.2214605/1‑94 (MQ=60)

                                                  CGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAA                                           >  SRR4302535.3862471/1‑94 (MQ=60)

                                                    TCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAA                                         <  SRR4302535.1196589/94‑1 (MQ=60)

                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                        >  SRR4302535.3199367/1‑94 (MQ=60)

                                                     CGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAAC                                        >  SRR4302535.4191895/1‑94 (MQ=60)

                                                      GAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACG                                       <  SRR4302535.2653717/94‑1 (MQ=60)

                                                       AAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGC                                      <  SRR4302535.4454642/94‑1 (MQ=60)

                                                        AGCACTGAACCGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCG                                     <  SRR4302535.4408804/94‑1 (MQ=60)

                                                        AGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCG                                     >  SRR4302535.1517024/1‑94 (MQ=60)

                                                          CACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                   >  SRR4302535.1336550/1‑94 (MQ=60)

                                                          CACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                   >  SRR4302535.1240533/1‑94 (MQ=60)

                                                          CACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGT                                   <  SRR4302535.2602575/94‑1 (MQ=60)

                                                           ACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTG                                  <  SRR4302535.2678060/94‑1 (MQ=60)

                                                           ACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTG                                  >  SRR4302535.1017408/1‑94 (MQ=60)

                                                            CTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGC                                 >  SRR4302535.4150929/1‑94 (MQ=60)

                                                            CTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGC                                 >  SRR4302535.672525/1‑94 (MQ=60)

                                                            CTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGC                                 <  SRR4302535.2205413/94‑1 (MQ=60)

                                                            CTGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGC                                 >  SRR4302535.4469449/1‑94 (MQ=60)

                                                             TGAAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCT                                <  SRR4302535.711435/94‑1 (MQ=60)

                                                               AAACGCCGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGG                              <  SRR4302535.1949570/94‑1 (MQ=60)

                                                                     CGCCGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGG                        <  SRR4302535.2052794/94‑1 (MQ=60)

                                                                       CCGCGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCA                      <  SRR4302535.3698088/94‑1 (MQ=60)

                                                                        CGTGAGCGCGGCCTTACCCCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAG                     <  SRR4302535.3312125/94‑1 (MQ=60)

                                                                        CGTGAGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAG                     <  SRR4302535.3524194/94‑1 (MQ=60)

                                                                            AGCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAA                 >  SRR4302535.3091335/1‑94 (MQ=60)

                                                                             GCGCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAA                >  SRR4302535.543707/1‑94 (MQ=60)

                                                                               GCGGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACG              <  SRR4302535.2761001/94‑1 (MQ=60)

                                                                                 GGCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTT            >  SRR4302535.114462/1‑94 (MQ=60)

                                                                                  GCCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTC           >  SRR4302535.809610/1‑94 (MQ=60)

                                                                                   CCTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCG          >  SRR4302535.1813569/1‑94 (MQ=60)

                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGC         >  SRR4302535.853112/1‑94 (MQ=60)

                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGC         >  SRR4302535.2965084/1‑94 (MQ=60)

                                                                                    CTTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGC         >  SRR4302535.4335872/1‑94 (MQ=60)

                                                                                     TTACACCGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCC        >  SRR4302535.2097902/1‑94 (MQ=60)

                                                                                           CGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT  >  SRR4302535.1647982/1‑94 (MQ=60)

                                                                                           CGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT  >  SRR4302535.3576720/1‑94 (MQ=60)

                                                                                           CGCTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTAGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT  >  SRR4302535.3063550/1‑94 (MQ=60)

ATTCACGATCTACAAACCCCTGAAGAACCTCACTCCGCACCGGGTATTCTCGTCGAAGCACTGAAACGCCGCCGTGAGCGCGGCCTTACACCGTTTACCGTGCTCTCCTGCGACAATATTCCCGACAATGGTCATGTGGTGAAAAACGCGGTGCTGGGAATGGCAGAAAAACGTTCGCCAGAACT  >  NC_000913/2264709‑2264893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`