Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,799 T→C V462A (GTA→GCA)  metL → aspartokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,7990TC99.6% 773.6 / ‑5.3 245V462A (GTA→GCA) metLaspartokinase II
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (114/130);  total (115/130)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 255 total aligned reads displayed.

TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888
                                                                                          |                                                                                         
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         <  1:765263/93‑1 (MQ=255)
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         >  1:1929566/1‑93 (MQ=255)
  gtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTg                                                                                       <  1:4075695/93‑1 (MQ=255)
    tgCATTACTGCGCGCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGtt                                                                                     <  2:1031699/92‑1 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    <  1:3034277/93‑1 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    <  1:1279252/93‑1 (MQ=255)
     gCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTc                                                                                    <  2:67368/93‑1 (MQ=255)
      cAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAGAACGCATCGGCCTGGCATTGTTCg                                                                                   >  1:2707606/1‑93 (MQ=255)
      cAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCg                                                                                   <  1:1664883/93‑1 (MQ=255)
        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                 <  2:86958/93‑1 (MQ=255)
        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                 >  2:3580132/1‑93 (MQ=255)
        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                 <  2:4662619/93‑1 (MQ=255)
           cTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAg                                                                              <  2:188363/93‑1 (MQ=255)
           cTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAg                                                                              <  1:2644242/93‑1 (MQ=255)
           cTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAg                                                                              <  2:2429336/93‑1 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             <  1:3812122/93‑1 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             >  1:2244963/1‑93 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTT‑CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAggg                                                                            <  1:919707/93‑1 (MQ=255)
              cgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGc                                                                           <  1:4580420/93‑1 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          <  1:241470/93‑1 (MQ=255)
                gaCCGCCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCaa                                                                         <  2:581753/92‑1 (MQ=255)
                cACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCaa                                                                         >  2:2462364/1‑93 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  1:93307/93‑1 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  2:1207493/93‑1 (MQ=255)
                  ccGCCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  2:3006416/93‑1 (MQ=255)
                   cGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAatat                                                                      <  2:4265725/93‑1 (MQ=255)
                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     <  1:4429673/93‑1 (MQ=255)
                     gCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCg                                                                    >  1:291754/1‑93 (MQ=255)
                      cccGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCgg                                                                   <  2:2690900/93‑1 (MQ=255)
                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                >  2:1589997/1‑93 (MQ=255)
                           ccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTccc                                                              <  1:1144642/93‑1 (MQ=255)
                           ccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTccc                                                              <  2:1068432/93‑1 (MQ=255)
                           ccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTccc                                                              >  2:3876577/1‑93 (MQ=255)
                                 tccTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGCGCAATATCGGTTCCCGTTGGc                                                        <  2:1821371/92‑1 (MQ=255)
                                 gCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGc                                                        >  1:3052237/1‑93 (MQ=255)
                                  ccTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCt                                                       <  2:2067850/93‑1 (MQ=255)
                                  ccTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCt                                                       <  1:359625/93‑1 (MQ=255)
                                  ccTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCt                                                       <  2:2490699/93‑1 (MQ=255)
                                  ccTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCt                                                       <  2:2734564/93‑1 (MQ=255)
                                      aTTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGaa                                                   <  2:3271852/93‑1 (MQ=255)
                                        tCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACt                                                 <  1:3889623/93‑1 (MQ=255)
                                         cAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTg                                                >  1:3744649/1‑93 (MQ=255)
                                         cAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTg                                                >  2:3681500/1‑93 (MQ=255)
                                         cAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTg                                                <  2:1472668/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              >  2:1365566/1‑93 (MQ=255)
                                             ggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCg                                            <  1:2750430/93‑1 (MQ=255)
                                               cTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAATGTTCgac                                          >  2:4179722/1‑91 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  1:1772834/1‑93 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  2:2783464/1‑93 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        <  1:2001156/93‑1 (MQ=255)
                                                  cATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGt                                       >  1:559026/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGGACTGTTCGCCCGTg                                      >  2:3941366/1‑93 (MQ=255)
                                                    taaGTCCGTCTTCCGCGCAGAAAAGCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTga                                     <  2:3864365/91‑1 (MQ=255)
                                                    tCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTga                                     <  1:205067/93‑1 (MQ=255)
                                                     cAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgag                                    >  1:3274403/1‑93 (MQ=255)
                                                      aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagc                                   <  1:4384930/93‑1 (MQ=255)
                                                      aGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagc                                   >  1:1409623/1‑93 (MQ=255)
                                                       gTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagca                                  <  2:3342811/93‑1 (MQ=255)
                                                       gTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagca                                  >  1:4195132/1‑93 (MQ=255)
                                                        tCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcag                                 >  1:2995344/1‑93 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                <  1:706211/93‑1 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                >  1:2792806/1‑93 (MQ=255)
                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                               >  1:1041332/1‑93 (MQ=255)
                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                               <  1:248905/93‑1 (MQ=255)
                                                          cGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagag                               <  2:2313705/93‑1 (MQ=255)
                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                              >  2:2591553/1‑93 (MQ=255)
                                                            tCTTCCTCGCAGAAAAACGCATCGGCCTAGCATTGTTCGGGAAGGGCAATATCGGTTTCCGTTGGCTGGAACTGTTCGGGCGTgaacagagca                             >  2:2861536/1‑93 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             <  2:4037188/93‑1 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             <  2:2181437/93‑1 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             <  1:2279787/93‑1 (MQ=255)
                                                             cTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAc                            <  2:808868/93‑1 (MQ=255)
                                                             cTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTACGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAc                            <  2:1518624/93‑1 (MQ=255)
                                                              ttCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACg                           >  1:3366629/1‑93 (MQ=255)
                                                                ccGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCt                         >  2:1337308/1‑93 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGGCCGTGAGCAGAGCACGCTTTc                      >  2:37013/1‑93 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      <  2:2238367/93‑1 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      <  2:3571804/93‑1 (MQ=255)
                                                                   cacAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      <  1:4512520/91‑1 (MQ=255)
                                                                       gAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCa                  >  1:1888891/1‑93 (MQ=255)
                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                >  2:2035999/1‑93 (MQ=255)
                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                >  1:3200135/1‑93 (MQ=255)
                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                >  2:3956763/1‑93 (MQ=255)
                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                <  2:1597930/93‑1 (MQ=255)
                                                                           aaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTa              >  2:2547963/1‑93 (MQ=255)
                                                                           aaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTa              >  2:441182/1‑93 (MQ=255)
                                                                           aaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACCTa              >  1:1447584/1‑93 (MQ=255)
                                                                            aCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAc             <  2:288781/93‑1 (MQ=255)
                                                                              gCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCg           >  2:520726/1‑93 (MQ=255)
                                                                              gCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCg           <  1:1506996/93‑1 (MQ=255)
                                                                               cATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCgg          >  1:3904990/1‑93 (MQ=255)
                                                                               cATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCgg          >  2:97845/1‑93 (MQ=255)
                                                                                aTCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGc         >  2:1199065/1‑93 (MQ=255)
                                                                                 tCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCt        >  2:346175/1‑93 (MQ=255)
                                                                                  cGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCtt       <  2:1367097/93‑1 (MQ=255)
                                                                                   ggCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCttt      <  1:1583911/93‑1 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     <  2:1980558/93‑1 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     >  2:4078647/1‑93 (MQ=255)
                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAg   >  2:3073488/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt  >  1:2153935/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt  >  1:1229982/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  NC_000913/4128706‑4128892
                                                                                             |                                                                                             
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGC                                                                                               <  SRR4302535.710813/94‑1 (MQ=60)
 CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCA                                                                                              >  SRR4302535.4377939/1‑94 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             >  SRR4302535.3609765/1‑94 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             >  SRR4302535.1943473/1‑94 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             <  SRR4302535.1199839/94‑1 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            <  SRR4302535.2221042/94‑1 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            <  SRR4302535.770653/94‑1 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          >  SRR4302535.2068479/1‑94 (MQ=60)
     GTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGT                                                                                          <  SRR4302535.4103580/94‑1 (MQ=60)
       GGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTC                                                                                        <  SRR4302535.1650865/94‑1 (MQ=60)
        GCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAGAACGCATCGGCCTGGCATTGTTCG                                                                                       >  SRR4302535.2726954/1‑94 (MQ=60)
        GCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCG                                                                                       <  SRR4302535.1288386/94‑1 (MQ=60)
        GCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCG                                                                                       <  SRR4302535.3055519/94‑1 (MQ=60)
         CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                      <  SRR4302535.1676915/94‑1 (MQ=60)
         CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                      <  SRR4302535.2261423/94‑1 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCGTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTCAG                                                                                  <  SRR4302535.4560892/94‑1 (MQ=60)
              CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 <  SRR4302535.2663179/94‑1 (MQ=60)
              CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 <  SRR4302535.272906/94‑1 (MQ=60)
              CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 <  SRR4302535.3933155/94‑1 (MQ=60)
              CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 <  SRR4302535.13436/94‑1 (MQ=60)
              CTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 >  SRR4302535.2260927/1‑94 (MQ=60)
              CGGCGCACCGGCCCGACCGAAAGCCTGATTCAGGTGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGG                                                                                 <  SRR4302535.3201048/94‑1 (MQ=60)
               TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                                <  SRR4302535.2685373/94‑1 (MQ=60)
               TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                                <  SRR4302535.3838429/94‑1 (MQ=60)
               TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTT‑CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGC                                                                               <  SRR4302535.926229/94‑1 (MQ=60)
                GCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGC                                                                               <  SRR4302535.3474466/94‑1 (MQ=60)
                 CGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCA                                                                              <  SRR4302535.4611617/94‑1 (MQ=60)
                  GCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAA                                                                             <  SRR4302535.243127/94‑1 (MQ=60)
                  GCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAA                                                                             >  SRR4302535.194645/1‑94 (MQ=60)
                     CCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATAT                                                                          <  SRR4302535.3000640/94‑1 (MQ=60)
                     CCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATAT                                                                          <  SRR4302535.93967/94‑1 (MQ=60)
                     CCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATAT                                                                          >  SRR4302535.2307806/1‑94 (MQ=60)
                      CGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATC                                                                         <  SRR4302535.3977168/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        >  SRR4302535.293795/1‑94 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302535.2026231/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302535.4460023/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302535.2682932/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302535.3274010/94‑1 (MQ=60)
                        GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                       >  SRR4302535.158530/1‑94 (MQ=60)
                          CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                     <  SRR4302535.783532/94‑1 (MQ=60)
                          CCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTT                                                                     >  SRR4302535.3669105/1‑94 (MQ=60)
                           CGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTC                                                                    >  SRR4302535.543249/1‑94 (MQ=60)
                              CCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                 >  SRR4302535.4573651/1‑94 (MQ=60)
                              CCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                 <  SRR4302535.1152802/94‑1 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATTGGTTCCCGTT                                                               >  SRR4302535.2585388/1‑94 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               >  SRR4302535.1173561/1‑94 (MQ=60)
                                 AAAGCCTGATTCAGGCGCTGCATCAGTCCGTCTCCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTG                                                              <  SRR4302535.388142/94‑1 (MQ=60)
                                   AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            >  SRR4302535.236643/1‑94 (MQ=60)
                                   AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            >  SRR4302535.3073613/1‑94 (MQ=60)
                                   AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            >  SRR4302535.3862530/1‑94 (MQ=60)
                                   AGCCTGATTCAGAGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            <  SRR4302535.1299171/94‑1 (MQ=60)
                                    GCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCGATATCGGTTCCCGTTGGCT                                                           <  SRR4302535.1427803/94‑1 (MQ=60)
                                    GCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCT                                                           >  SRR4302535.4264699/1‑94 (MQ=60)
                                     CCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCGCGTTGGCTG                                                          >  SRR4302535.438937/1‑94 (MQ=60)
                                     CCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTG                                                          <  SRR4302535.362114/94‑1 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         <  SRR4302535.926560/94‑1 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         <  SRR4302535.3930892/94‑1 (MQ=60)
                                        GATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAA                                                       >  SRR4302535.1934317/1‑94 (MQ=60)
                                           TCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTG                                                    >  SRR4302535.3770557/1‑94 (MQ=60)
                                           TCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTG                                                    <  SRR4302535.3916292/94‑1 (MQ=60)
                                             AGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTT                                                  <  SRR4302535.2827287/94‑1 (MQ=60)
                                                GGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGC                                               <  SRR4302535.2770037/94‑1 (MQ=60)
                                                  CTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCC                                             >  SRR4302535.1785682/1‑94 (MQ=60)
                                                    GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                           <  SRR4302535.2015573/94‑1 (MQ=60)
                                                    GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                           >  SRR4302535.562933/1‑94 (MQ=60)
                                                     CATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTG                                          >  SRR4302535.448296/1‑94 (MQ=60)
                                                       TCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG                                        <  SRR4302535.206474/94‑1 (MQ=60)
                                                       TCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG                                        >  SRR4302535.3297147/1‑94 (MQ=60)
                                                       TCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAG                                        >  SRR4302535.4168755/1‑94 (MQ=60)
                                                        CAGTCCGTGTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGC                                       <  SRR4302535.3055603/94‑1 (MQ=60)
                                                        CAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGC                                       >  SRR4302535.1419688/1‑94 (MQ=60)
                                                         AGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCA                                      >  SRR4302535.4223839/1‑94 (MQ=60)
                                                         AGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCA                                      <  SRR4302535.4414931/94‑1 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAG                                     >  SRR4302535.1810106/1‑94 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAG                                     <  SRR4302535.578013/94‑1 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAG                                     <  SRR4302535.2169829/94‑1 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAG                                     >  SRR4302535.3016341/1‑94 (MQ=60)
                                                           TCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGA                                    >  SRR4302535.2812679/1‑94 (MQ=60)
                                                           TCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGA                                    <  SRR4302535.1221508/94‑1 (MQ=60)
                                                            CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                   >  SRR4302535.1048717/1‑94 (MQ=60)
                                                            CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                   <  SRR4302535.711172/94‑1 (MQ=60)
                                                            CCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAG                                   <  SRR4302535.1638301/94‑1 (MQ=60)
                                                             CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                  <  SRR4302535.250616/94‑1 (MQ=60)
                                                             CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                  >  SRR4302535.599678/1‑94 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                 >  SRR4302535.299411/1‑94 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302535.2296071/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302535.2573498/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                >  SRR4302535.2775339/1‑94 (MQ=60)
                                                                CTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACG                               >  SRR4302535.3390056/1‑94 (MQ=60)
                                                                CTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACG                               <  SRR4302535.2036815/94‑1 (MQ=60)
                                                                CTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACG                               <  SRR4302535.4476584/94‑1 (MQ=60)
                                                                    CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTT                           <  SRR4302535.1003477/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302535.1880979/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302535.3057250/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302535.2778074/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302535.3369319/94‑1 (MQ=60)
                                                                      CACAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         <  SRR4302535.4543467/94‑1 (MQ=60)
                                                                        CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                       >  SRR4302535.1476958/1‑94 (MQ=60)
                                                                        CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                       >  SRR4302535.905256/1‑94 (MQ=60)
                                                                         AGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCA                      >  SRR4302535.1902497/1‑94 (MQ=60)
                                                                         AGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCA                      <  SRR4302535.3709712/94‑1 (MQ=60)
                                                                          GAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAC                     <  SRR4302535.1707882/94‑1 (MQ=60)
                                                                          GAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAC                     >  SRR4302535.993871/1‑94 (MQ=60)
                                                                           AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                    <  SRR4302535.1073140/94‑1 (MQ=60)
                                                                           AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                    >  SRR4302535.3823214/1‑94 (MQ=60)
                                                                           AAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACG                    >  SRR4302535.3222292/1‑94 (MQ=60)
                                                                             AAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTA                  >  SRR4302535.1247401/1‑94 (MQ=60)
                                                                             AAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACCTA                  >  SRR4302535.1457934/1‑94 (MQ=60)
                                                                               ACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACC                >  SRR4302535.1910731/1‑94 (MQ=60)
                                                                               ACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACC                <  SRR4302535.3272291/94‑1 (MQ=60)
                                                                                 GCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGG              <  SRR4302535.1517832/94‑1 (MQ=60)
                                                                                 GCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGG              >  SRR4302535.3931737/1‑94 (MQ=60)
                                                                                      GGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTG         <  SRR4302535.1595341/94‑1 (MQ=60)
                                                                                        CCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAG       >  SRR4302535.3017380/1‑94 (MQ=60)
                                                                                        CCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAG       <  SRR4302535.2482663/94‑1 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      >  SRR4302535.1476507/1‑94 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      <  SRR4302535.75517/94‑1 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      >  SRR4302535.1238772/1‑94 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      >  SRR4302535.2169415/1‑94 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGACT      <  SRR4302535.4455373/94‑1 (MQ=60)
                                                                                          TGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTT     >  SRR4302535.1146187/1‑94 (MQ=60)
                                                                                           GGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTT    <  SRR4302535.3514624/94‑1 (MQ=60)
                                                                                           GGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGGTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTT    <  SRR4302535.3089552/94‑1 (MQ=60)
                                                                                             CATTGTTCGTTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  <  SRR4302535.3364236/94‑1 (MQ=60)
                                                                                             CATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  <  SRR4302535.3985254/94‑1 (MQ=60)
                                                                                             CATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  <  SRR4302535.453545/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  NC_000913/4128706‑4128892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑