Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,130,739 A→G T182A (ACT→GCT)  metF → 5,10‑methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,130,7390AG100.0% 647.5 / NA 203T182A (ACT→GCT) metF5,10‑methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (98/105);  total (98/105)

Only 100 of 211 total aligned reads displayed.

TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130649‑4130828
                                                                                          |                                                                                         
tATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCt                                                                                         >  1:1649402/1‑93 (MQ=255)
 aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTc                                                                                        <  1:2318203/93‑1 (MQ=255)
 aTCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTc                                                                                        >  2:311477/1‑93 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                       <  1:3380040/93‑1 (MQ=255)
  tCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCa                                                                                       <  2:2725244/93‑1 (MQ=255)
    cGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGt                                                                                     <  1:4667129/93‑1 (MQ=255)
      gAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttc                                                                                   <  1:3091796/93‑1 (MQ=255)
       aaGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttct                                                                                  <  1:1178042/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                 <  2:3478717/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttctt                                                                                 <  1:2204383/93‑1 (MQ=255)
        aGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGttctt                                                                                 >  1:3906328/1‑93 (MQ=255)
         gTTCGCCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTAAGttcttc                                                                                <  2:4547377/93‑1 (MQ=255)
         gTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttc                                                                                <  1:2549592/93‑1 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                               <  1:1211160/93‑1 (MQ=255)
          ttCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttct                                                                               <  1:979620/93‑1 (MQ=255)
           tCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGttcttctt                                                                              <  2:805196/93‑1 (MQ=255)
              ccgggAAGCAAAAAGCGCTCACGCTGATTTGCTTAATCTGAAACGCAAAGTGGAGGCCGGATCGAACGGCGCGATTGATCAGTTCTTCTTCGa                                                                           <  2:855156/90‑1 (MQ=255)
               ccGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAt                                                                          >  2:2243401/1‑93 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                         >  2:3751383/1‑93 (MQ=255)
                cGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATg                                                                         <  2:6793/93‑1 (MQ=255)
                  gAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTc                                                                       >  1:227118/1‑93 (MQ=255)
                   aaGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCg                                                                      >  1:709932/1‑93 (MQ=255)
                      cAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   >  1:1601484/1‑93 (MQ=255)
                      cAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGaaa                                                                   <  1:3625761/93‑1 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  <  2:388561/93‑1 (MQ=255)
                       aaaaaGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAg                                                                  <  2:2086099/93‑1 (MQ=255)
                        aaaaGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGc                                                                 >  1:3263597/1‑93 (MQ=255)
                            gcgcTCAGGCGGATTTGCTTAATCTGAAGCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTAcc                                                             <  1:2610749/93‑1 (MQ=255)
                             cgcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCt                                                            <  1:4038394/93‑1 (MQ=255)
                              gcTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTg                                                           <  2:3419969/93‑1 (MQ=255)
                               cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          <  1:1819597/93‑1 (MQ=255)
                               cTCAGGCGGATTTGCTTAATCTGAAACGCAAAGGGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGc                                                          >  2:766497/1‑93 (MQ=255)
                                tCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCcgcg                                                         >  2:4356566/1‑93 (MQ=255)
                                   ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGttt                                                      >  2:4002089/1‑93 (MQ=255)
                                   ggCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGttt                                                      >  1:294698/1‑93 (MQ=255)
                                     cGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTc                                                    <  2:184753/93‑1 (MQ=255)
                                      ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCg                                                   >  1:4618482/1‑93 (MQ=255)
                                      ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCg                                                   >  1:3780461/1‑93 (MQ=255)
                                      ggATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCg                                                   <  1:1098465/93‑1 (MQ=255)
                                         tttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGa                                                >  1:4155322/1‑93 (MQ=255)
                                          ttGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCATTTCTTCTTCGATGTCTAAAGCTACCTTCGTTTTCGTGAc                                               >  1:1534533/1‑93 (MQ=255)
                                           tGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAcc                                              <  1:74828/93‑1 (MQ=255)
                                            gCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCg                                             >  2:2475073/1‑93 (MQ=255)
                                            gCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCg                                             >  1:1440077/1‑93 (MQ=255)
                                            gCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCg                                             <  1:348691/93‑1 (MQ=255)
                                             cTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGc                                            >  1:3349241/1‑93 (MQ=255)
                                             cTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGc                                            >  1:1087565/1‑93 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          >  2:1620512/1‑93 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          <  2:3812289/93‑1 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          <  1:2103364/93‑1 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtg                                          <  1:2775845/93‑1 (MQ=255)
                                               tAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTCCGTTTTCGTGACCGCtg                                          >  2:2607796/1‑93 (MQ=255)
                                                aaTCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCtgt                                         >  1:626904/1‑93 (MQ=255)
                                                    tGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTAt                                     <  2:3348483/93‑1 (MQ=255)
                                                      aaaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcg                                   <  1:722568/93‑1 (MQ=255)
                                                      aaaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcg                                   >  1:3860118/1‑93 (MQ=255)
                                                      aaaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcg                                   >  1:3693166/1‑93 (MQ=255)
                                                       aaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcgg                                  >  2:4433831/1‑93 (MQ=255)
                                                       aaCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcgg                                  >  2:2848760/1‑93 (MQ=255)
                                                        aCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggc                                 <  2:1688959/93‑1 (MQ=255)
                                                        aCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggc                                 <  2:1924120/93‑1 (MQ=255)
                                                         cGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcg                                <  1:1566973/93‑1 (MQ=255)
                                                          gCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcgg                               <  2:2991435/93‑1 (MQ=255)
                                                          gCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATcggcgg                               <  2:4669605/93‑1 (MQ=255)
                                                           cAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCggg                              >  2:2445375/1‑93 (MQ=255)
                                                            aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTTGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGc                             >  1:2640244/1‑93 (MQ=255)
                                                            aaaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCgggg                             >  1:1470231/1‑92 (MQ=255)
                                                             aaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCTATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCa                            >  2:2330093/1‑93 (MQ=255)
                                                             aaGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCa                            <  1:2919121/93‑1 (MQ=255)
                                                                tGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTg                         >  1:822833/1‑93 (MQ=255)
                                                                 agATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGa                        <  1:4501305/92‑1 (MQ=255)
                                                                  gATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAt                       <  2:1489698/93‑1 (MQ=255)
                                                                   gtGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtg                      <  2:2493599/92‑1 (MQ=255)
                                                                   aTGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGtg                      >  2:1502385/1‑93 (MQ=255)
                                                                   aTGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGAATTGAtg                      >  1:2358136/1‑93 (MQ=255)
                                                                    tGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAtgt                     <  2:3364684/93‑1 (MQ=255)
                                                                     gCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGtgtg                    >  1:3038491/1‑93 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGAGTGGTGTGGaa                 >  1:1066076/1‑93 (MQ=255)
                                                                        ggAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaa                 <  2:3043275/93‑1 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTTATGTGGgaa                >  2:1817617/1‑93 (MQ=255)
                                                                         gAGCCAACCGCGCGATTGCTCAGTTCCTCTTCGATGTCGAAATCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGaaa                <  2:1381516/93‑1 (MQ=255)
                                                                          aGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAat               >  1:1937754/1‑93 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGATGTGGAAatt              >  1:564696/1‑93 (MQ=255)
                                                                           gCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAatt              >  2:3366690/1‑93 (MQ=255)
                                                                             cAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTTATGTGGAAaatat            >  1:4209582/1‑93 (MQ=255)
                                                                              aaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACGGCTGTGTATCGGCGGGCATTGATGTGGAAattatt           <  2:3631885/93‑1 (MQ=255)
                                                                              aaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAattatt           >  1:1026899/1‑93 (MQ=255)
                                                                              aaCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAattatc           >  1:2903051/1‑92 (MQ=255)
                                                                               aCCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTc          >  2:1147738/1‑93 (MQ=255)
                                                                                ccGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTcc         <  2:4482796/93‑1 (MQ=255)
                                                                                ccGCGCGATTGCTCAGTTCTTCTTCGATGACGAAAGCTACCTGCGTAATCGTCACCGCTGTGTATCGGCGGGCATTGATGTGGACATTATTcc         <  2:4047840/93‑1 (MQ=255)
                                                                                  gcgcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGATGTGGGAATTATTTCgg       >  2:3261147/1‑93 (MQ=255)
                                                                                    gcgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGa     >  1:1200330/1‑93 (MQ=255)
                                                                                     cgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGaa    <  1:449312/93‑1 (MQ=255)
                                                                                     cgATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGaa    <  1:3236785/93‑1 (MQ=255)
                                                                                      gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTTCGGGGAt   >  2:4118357/1‑93 (MQ=255)
                                                                                      gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCGGGAAt   >  1:4390800/1‑93 (MQ=255)
                                                                                      gATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAt   <  2:3762615/93‑1 (MQ=255)
                                                                                       aTTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAAATATTCCGGGGAtt  >  2:4133868/1‑93 (MQ=255)
                                                                                       aTTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGGAtt  >  1:1286886/1‑93 (MQ=255)
                                                                                          |                                                                                         
TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATT  >  NC_000913/4130649‑4130828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTT  >  NC_000913/4130646‑4130830
                                                                                             |                                                                                           
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTG                                                                                             <  SRR4302535.1559929/94‑1 (MQ=60)
 CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                            >  SRR4302535.2883678/1‑94 (MQ=60)
 CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                            >  SRR4302535.2704475/1‑94 (MQ=60)
 CGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGC                                                                                            >  SRR4302535.2224313/1‑94 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                           >  SRR4302535.1661331/1‑94 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                           <  SRR4302535.3608259/94‑1 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                           <  SRR4302535.1402854/94‑1 (MQ=60)
  GTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCT                                                                                           <  SRR4302535.491363/94‑1 (MQ=60)
   TATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTC                                                                                          >  SRR4302535.1503046/1‑94 (MQ=60)
    ATCCGGAAGTTCACCCGGAGGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCA                                                                                         >  SRR4302535.1917490/1‑94 (MQ=60)
    ATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCA                                                                                         <  SRR4302535.2334795/94‑1 (MQ=60)
     TCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAG                                                                                        <  SRR4302535.3658505/94‑1 (MQ=60)
     TCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAG                                                                                        <  SRR4302535.3403541/94‑1 (MQ=60)
       CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                      <  SRR4302535.1193280/94‑1 (MQ=60)
       CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                      >  SRR4302535.3612704/1‑94 (MQ=60)
       CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                      <  SRR4302535.4698672/94‑1 (MQ=60)
       CGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTT                                                                                      <  SRR4302535.2772397/94‑1 (MQ=60)
        GGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTC                                                                                     >  SRR4302535.3159840/1‑94 (MQ=60)
         GAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCT                                                                                    <  SRR4302535.3261877/94‑1 (MQ=60)
         GAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCT                                                                                    <  SRR4302535.3113326/94‑1 (MQ=60)
          AAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTT                                                                                   <  SRR4302535.1186445/94‑1 (MQ=60)
          AAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGACAACCGCGCGATTGCTCAGTTCTT                                                                                   >  SRR4302535.3933091/1‑94 (MQ=60)
           AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                  <  SRR4302535.2220085/94‑1 (MQ=60)
           AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                  >  SRR4302535.3499063/1‑94 (MQ=60)
           AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                  <  SRR4302535.4046567/94‑1 (MQ=60)
           AGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTC                                                                                  >  SRR4302535.4669280/1‑94 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                 <  SRR4302535.2567944/94‑1 (MQ=60)
            GTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCT                                                                                 >  SRR4302535.4295677/1‑94 (MQ=60)
             TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTT                                                                                <  SRR4302535.986583/94‑1 (MQ=60)
             TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTT                                                                                <  SRR4302535.1219857/94‑1 (MQ=60)
             TTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTT                                                                                <  SRR4302535.1558512/94‑1 (MQ=60)
              TCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTC                                                                               <  SRR4302535.2261520/94‑1 (MQ=60)
                 CCCGGAAGCAAACGCCGCCCAGGCGGCTTTGCCTCATCTGAACCCCAAAGTGGATGCCGGAGCCACCCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                            <  SRR4302535.336772/94‑1 (MQ=34)
                 CCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                            <  SRR4302535.1613703/94‑1 (MQ=60)
                 CCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGAT                                                                            <  SRR4302535.2621433/94‑1 (MQ=60)
                  CCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATG                                                                           <  SRR4302535.1209192/94‑1 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                         >  SRR4302535.3833965/1‑94 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                         >  SRR4302535.724955/1‑94 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                         <  SRR4302535.4149441/94‑1 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                         >  SRR4302535.3094880/1‑94 (MQ=60)
                    GGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTC                                                                         >  SRR4302535.228680/1‑94 (MQ=60)
                     GAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAGGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCG                                                                        >  SRR4302535.4602977/1‑94 (MQ=60)
                     GAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCG                                                                        >  SRR4302535.714916/1‑94 (MQ=60)
                       AGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAA                                                                      <  SRR4302535.2166009/94‑1 (MQ=60)
                        GCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAA                                                                     >  SRR4302535.1613039/1‑94 (MQ=60)
                         CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                    <  SRR4302535.73787/94‑1 (MQ=60)
                         CAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAG                                                                    <  SRR4302535.3650889/94‑1 (MQ=60)
                          AAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                   >  SRR4302535.3759844/1‑94 (MQ=60)
                          AAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGC                                                                   >  SRR4302535.3286263/1‑94 (MQ=60)
                              AGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACC                                                               <  SRR4302535.82761/94‑1 (MQ=60)
                               GCGCTCAGGCGGATTTGCTTAATCTGAAGCGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCT                                                              <  SRR4302535.2629479/94‑1 (MQ=60)
                               GCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCT                                                              <  SRR4302535.1406672/94‑1 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                             <  SRR4302535.4066039/94‑1 (MQ=60)
                                CGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTG                                                             >  SRR4302535.2940393/1‑94 (MQ=60)
                                 GCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGC                                                            <  SRR4302535.2334567/94‑1 (MQ=60)
                                  CTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCG                                                           <  SRR4302535.1832743/94‑1 (MQ=60)
                                     AGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTT                                                        >  SRR4302535.296754/1‑94 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                     >  SRR4302535.3806553/1‑94 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                     <  SRR4302535.3727116/94‑1 (MQ=60)
                                        CGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCG                                                     >  SRR4302535.4649866/1‑94 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                    <  SRR4302535.1106287/94‑1 (MQ=60)
                                         GGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGT                                                    >  SRR4302535.2647197/1‑94 (MQ=60)
                                          GATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTG                                                   >  SRR4302535.145297/1‑94 (MQ=60)
                                           ATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGA                                                  >  SRR4302535.4183769/1‑94 (MQ=60)
                                            TTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCATTTCTTCTTCGATGTCTAAAGCTACCTTCGTTTTCGTGAC                                                 >  SRR4302535.1545587/1‑94 (MQ=60)
                                            TTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAC                                                 >  SRR4302535.914262/1‑94 (MQ=60)
                                            TTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAC                                                 <  SRR4302535.3011138/94‑1 (MQ=60)
                                             TTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACC                                                >  SRR4302535.1095491/1‑94 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                               >  SRR4302535.150311/1‑94 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                               <  SRR4302535.75375/94‑1 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                               >  SRR4302535.1820228/1‑94 (MQ=60)
                                              TGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCG                                               >  SRR4302535.1450375/1‑94 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                              >  SRR4302535.3372573/1‑94 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                              <  SRR4302535.351092/94‑1 (MQ=60)
                                               GCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGC                                              >  SRR4302535.1095300/1‑94 (MQ=60)
                                                CTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCT                                             <  SRR4302535.2091155/94‑1 (MQ=60)
                                                 TTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTG                                            <  SRR4302535.4529526/94‑1 (MQ=60)
                                                  TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                           <  SRR4302535.2118534/94‑1 (MQ=60)
                                                  TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                           <  SRR4302535.2795607/94‑1 (MQ=60)
                                                  TAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGT                                           >  SRR4302535.631269/1‑94 (MQ=60)
                                                       TGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATC                                      >  SRR4302535.493240/1‑94 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                     <  SRR4302535.2296380/94‑1 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                     >  SRR4302535.3886683/1‑94 (MQ=60)
                                                        GAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCG                                     >  SRR4302535.3718728/1‑94 (MQ=60)
                                                         AAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGG                                    <  SRR4302535.727642/94‑1 (MQ=60)
                                                            CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                 >  SRR4302535.1052304/1‑94 (MQ=60)
                                                            CGCAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGG                                 <  SRR4302535.1578279/94‑1 (MQ=60)
                                                             GCNANNNNGATGCCGGAGCCANNNGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGG                                <  SRR4302535.4228835/94‑1 (MQ=60)
                                                              CAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTTGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGC                               >  SRR4302535.2659158/1‑94 (MQ=60)
                                                              CAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGG                               >  SRR4302535.1480761/1‑94 (MQ=60)
                                                              CAAAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGC                               >  SRR4302535.599713/1‑94 (MQ=60)
                                                                AAGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCAT                             <  SRR4302535.2939721/94‑1 (MQ=60)
                                                                 AGTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATT                            <  SRR4302535.3396117/94‑1 (MQ=60)
                                                                  GTGGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTG                           >  SRR4302535.828600/1‑94 (MQ=60)
                                                                    AGATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAT                         <  SRR4302535.4532216/94‑1 (MQ=60)
                                                                     GATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATT                        >  SRR4302535.4649576/1‑94 (MQ=60)
                                                                     GATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATG                        <  SRR4302535.4509779/94‑1 (MQ=60)
                                                                     GATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGAATTGATG                        >  SRR4302535.2375052/1‑94 (MQ=60)
                                                                      ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGggttgatgg                       >  SRR4302535.1080742/1‑85 (MQ=60)
                                                                      ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGATGG                       >  SRR4302535.4218389/1‑94 (MQ=60)
                                                                      ATGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGT                       <  SRR4302535.1115561/94‑1 (MQ=60)
                                                                       TGCCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGGTGTG                      >  SRR4302535.3059773/1‑94 (MQ=60)
                                                                        GGCGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGG                     <  SRR4302535.2374123/94‑1 (MQ=60)
                                                                          CGGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGAGTGGTGTGGAA                   >  SRR4302535.1073660/1‑94 (MQ=60)
                                                                           GGAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGGTGTGGAAA                  >  SRR4302535.1282922/1‑94 (MQ=60)
                                                                            GAGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAAT                 >  SRR4302535.1951704/1‑94 (MQ=60)
                                                                             AGCCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCGTTGATGTGGAAATT                >  SRR4302535.568650/1‑94 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTTATGTGGAAAATAT              >  SRR4302535.4238391/1‑94 (MQ=60)
                                                                               CCAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTAT              <  SRR4302535.2555284/94‑1 (MQ=60)
                                                                                CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCCTTGATGTGGCAATTATT             >  SRR4302535.1601814/1‑94 (MQ=60)
                                                                                CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGTTGTGGAAATTATC             >  SRR4302535.2923571/1‑94 (MQ=60)
                                                                                CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATT             >  SRR4302535.293751/1‑94 (MQ=60)
                                                                                CAACCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATT             >  SRR4302535.1034191/1‑94 (MQ=60)
                                                                                   CCGCGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTTATGTGGGAATTGTTCCG          >  SRR4302535.3006661/1‑94 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGA       >  SRR4302535.1208931/1‑94 (MQ=60)
                                                                                      CGCGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGA       <  SRR4302535.3926417/94‑1 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGGAATTATTCCGGGAAT     >  SRR4302535.4420844/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGAAT     >  SRR4302535.698798/1‑94 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT     <  SRR4302535.3259254/94‑1 (MQ=60)
                                                                                        CGATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAAT     <  SRR4302535.452459/94‑1 (MQ=60)
                                                                                         GATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTTCGGGAATT    >  SRR4302535.3330644/1‑94 (MQ=60)
                                                                                         GATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGGATT    >  SRR4302535.1296055/1‑94 (MQ=60)
                                                                                          ATTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTT   <  SRR4302535.2883022/94‑1 (MQ=60)
                                                                                           TTGCTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTGGTGTGGAAATTATTCCGGGGATTTT  >  SRR4302535.2541463/1‑94 (MQ=60)
                                                                                             |                                                                                           
GCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTCCGGGAATTTT  >  NC_000913/4130646‑4130830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑