Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,134,509 A→G E274E (GAA→GAG)  b3943 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,134,5090AG100.0% 530.5 / NA 167E274E (GAA→GAG) b3943hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (66/101);  total (66/101)

Only 100 of 177 total aligned reads displayed.

CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598
                                                                                          |                                                                                         
cAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAggg                                                                                         <  2:2650759/93‑1 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        <  1:1533262/93‑1 (MQ=255)
 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATACGGGGGCCGTTGAGGGt                                                                                        >  1:4645354/1‑93 (MQ=255)
   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      <  2:1141474/93‑1 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAgg                                                                                     >  2:819155/1‑92 (MQ=255)
    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     <  2:4537013/93‑1 (MQ=255)
     ttGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCg                                                                                    >  1:1781321/1‑93 (MQ=255)
      tGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCgg                                                                                   <  2:1525460/93‑1 (MQ=255)
       gCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGt                                                                                  >  2:2354707/1‑93 (MQ=255)
        ccAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTa                                                                                 <  1:3729951/93‑1 (MQ=255)
           gCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGCATCCGGGGGCCGTTGAGGGGAGCGGTattg                                                                              >  2:1826783/1‑93 (MQ=255)
           gCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattg                                                                              >  2:1625878/1‑93 (MQ=255)
            cTTAAGCACACTCGCCGTTCCCGTTTGCGGCGTACTCTTTTTCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgt                                                                             >  1:2416001/1‑93 (MQ=255)
               aaGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGGGGTattgtgct                                                                          >  2:3671961/1‑93 (MQ=255)
                  cacaCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgat                                                                       >  2:4637979/1‑93 (MQ=255)
                   acacTGGCCGTTCCCGTTTGCGGCTTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGACGGTAGCGGTattgtgctgatt                                                                      >  2:782849/1‑93 (MQ=255)
                   acacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgatt                                                                      >  2:2061570/1‑93 (MQ=255)
                   acacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgatt                                                                      >  2:1012530/1‑93 (MQ=255)
                   acacTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgatt                                                                      <  1:670895/93‑1 (MQ=255)
                    cacGGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattg                                                                     <  1:3807170/93‑1 (MQ=255)
                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  <  2:4379996/93‑1 (MQ=255)
                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  <  1:750587/93‑1 (MQ=255)
                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  <  2:270997/93‑1 (MQ=255)
                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  <  2:2272829/93‑1 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAGTCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 <  1:2506962/93‑1 (MQ=255)
                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 <  1:3573062/93‑1 (MQ=255)
                          cggTTCCCGTGTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTgg                                                               <  2:4623342/91‑1 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              <  2:1484258/93‑1 (MQ=255)
                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              >  1:535216/1‑93 (MQ=255)
                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            <  1:2027606/93‑1 (MQ=255)
                             gtCCCGTTTGCGGCGGACTCTTTTCCTGGTGGCTGCTCGGCGAGCATCCGCGGGCCGTTGAGGGCAGCGGTAGTGTGCTGATTGGTCTGGCAc                                                            >  2:433803/2‑93 (MQ=255)
                               cccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTg                                                          >  1:4192210/1‑93 (MQ=255)
                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                                         <  1:3219916/93‑1 (MQ=255)
                                 cGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgc                                                        <  1:231854/93‑1 (MQ=255)
                                 cGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgc                                                        >  1:4237902/1‑93 (MQ=255)
                                   tttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgcgc                                                      <  1:2399913/93‑1 (MQ=255)
                                    ttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCt                                                     <  1:841562/93‑1 (MQ=255)
                                    ttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCt                                                     <  2:4193636/93‑1 (MQ=255)
                                      gcggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTgg                                                   >  2:925309/1‑93 (MQ=255)
                                       cggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGt                                                  >  2:3870919/1‑93 (MQ=255)
                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGGTGGTGAGc                                              >  2:195182/1‑93 (MQ=255)
                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGc                                              >  2:4005318/1‑93 (MQ=255)
                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGc                                              <  1:1140187/93‑1 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATTCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             <  2:2732660/93‑1 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  2:2187479/1‑93 (MQ=255)
                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGGATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  1:2626821/1‑93 (MQ=255)
                                             tctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            <  2:2831426/92‑1 (MQ=255)
                                             aCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            >  1:1710860/1‑93 (MQ=255)
                                              ctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGt                                           >  1:2263025/1‑93 (MQ=255)
                                                ctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         >  2:2908940/1‑93 (MQ=255)
                                                ctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         >  1:2181119/1‑93 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        >  2:2964187/1‑93 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        <  1:2687916/93‑1 (MQ=255)
                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        >  1:291527/1‑93 (MQ=255)
                                                     ccTGGTGGCTGCTCGGCTAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaa                                    <  1:2993580/93‑1 (MQ=255)
                                                     ccTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaa                                    <  1:3345948/93‑1 (MQ=255)
                                                     ccTGGTGGCTGCTCGGCGAGAAGCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCGGGTGAGCCGTAAGaaaa                                    <  2:2519338/93‑1 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   <  2:173410/93‑1 (MQ=255)
                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   <  2:2124738/93‑1 (MQ=255)
                                                        ggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaag                                 >  2:807408/1‑93 (MQ=255)
                                                         gtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga                                <  1:2843399/93‑1 (MQ=255)
                                                         gtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga                                <  1:807095/93‑1 (MQ=255)
                                                         gtggCTGCTCGGCGAGAATCCGGGGGCCGTGGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaaga                                <  1:261937/93‑1 (MQ=255)
                                                          tggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa                               <  1:753790/93‑1 (MQ=255)
                                                          tggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa                               <  2:3064094/93‑1 (MQ=255)
                                                          tggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa                               <  2:1555554/93‑1 (MQ=255)
                                                           ggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaag                              <  1:2542110/93‑1 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:3777660/93‑1 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:3960896/93‑1 (MQ=255)
                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:4172/93‑1 (MQ=255)
                                                             ctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            >  2:2521795/1‑93 (MQ=255)
                                                              tgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCg                           <  2:319201/93‑1 (MQ=255)
                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGGAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          >  2:1873585/1‑93 (MQ=255)
                                                                ctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc                         >  2:3470240/1‑93 (MQ=255)
                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:1419685/93‑1 (MQ=255)
                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  1:3213930/93‑1 (MQ=255)
                                                                  cGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAg                       <  1:294452/93‑1 (MQ=255)
                                                                    gCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgtg                     <  1:2108349/93‑1 (MQ=255)
                                                                    gCGAGAAGCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgtg                     <  1:3150402/93‑1 (MQ=255)
                                                                     cGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAgtgt                    <  2:1005988/93‑1 (MQ=255)
                                                                       agaATCTGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTCGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaa                  <  1:890358/93‑1 (MQ=255)
                                                                       agaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaa                  >  2:927318/1‑93 (MQ=255)
                                                                        gaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACCGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaa                 <  2:2416091/93‑1 (MQ=255)
                                                                         aaTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaa                <  2:4265979/93‑1 (MQ=255)
                                                                          aTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaaa               <  2:869742/93‑1 (MQ=255)
                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  1:2118859/93‑1 (MQ=255)
                                                                           cccGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCCCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  2:3981776/92‑1 (MQ=255)
                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:2627168/93‑1 (MQ=255)
                                                                              gggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAt           >  1:4350660/1‑93 (MQ=255)
                                                                              gggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAt           <  1:1015003/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          <  1:3428613/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          <  1:4281389/93‑1 (MQ=255)
                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          <  1:1899006/93‑1 (MQ=255)
                                                                                  gctgTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGa       <  2:4479126/90‑1 (MQ=255)
                                                                                  gCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGa       <  1:1489811/93‑1 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:3763191/93‑1 (MQ=255)
                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:3638779/93‑1 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  2:4086101/1‑93 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  2:613139/1‑93 (MQ=255)
                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  2:2811813/1‑93 (MQ=255)
                                                                                          |                                                                                         
CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  >  NC_000913/4134416‑4134602
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               <  SRR4302535.1484876/94‑1 (MQ=60)
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAG                                                                                               >  SRR4302535.2780574/1‑94 (MQ=60)
 GCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGG                                                                                              <  SRR4302535.700279/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             <  SRR4302535.3159991/94‑1 (MQ=60)
  CCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGG                                                                                             >  SRR4302535.756746/1‑94 (MQ=60)
   CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATACGGGGGCCGTTGAGGGT                                                                                            >  SRR4302535.4676833/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           >  SRR4302535.1470943/1‑94 (MQ=60)
    AGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTA                                                                                           <  SRR4302535.1544310/94‑1 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        >  SRR4302535.3410114/1‑94 (MQ=60)
       ATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCG                                                                                        >  SRR4302535.1794212/1‑94 (MQ=60)
         TGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGT                                                                                      >  SRR4302535.2540333/1‑94 (MQ=60)
           CCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTAT                                                                                    <  SRR4302535.3755781/94‑1 (MQ=60)
             AGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTG                                                                                  >  SRR4302535.567206/1‑94 (MQ=60)
              GCTTAAGCACACTCGCCGTTCCCGTTTGCGGCGTACTCTTTTTCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGT                                                                                 >  SRR4302535.2433392/1‑94 (MQ=60)
               CTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTG                                                                                >  SRR4302535.2038841/1‑94 (MQ=60)
                    GCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGAT                                                                           <  SRR4302535.843728/94‑1 (MQ=60)
                      ACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         >  SRR4302535.2738560/1‑94 (MQ=60)
                      ACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         <  SRR4302535.675578/94‑1 (MQ=60)
                      ACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTG                                                                         <  SRR4302535.2912398/94‑1 (MQ=60)
                       CACGGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGT                                                                        <  SRR4302535.3833426/94‑1 (MQ=60)
                          TGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCT                                                                     <  SRR4302535.755878/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAGTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302535.2525036/94‑1 (MQ=60)
                           GGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTG                                                                    <  SRR4302535.3597839/94‑1 (MQ=60)
                             CCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGC                                                                  >  SRR4302535.538956/1‑94 (MQ=60)
                                TTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACT                                                               <  SRR4302535.2042220/94‑1 (MQ=60)
                                 TCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTG                                                              >  SRR4302535.4220899/1‑94 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302535.3071765/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302535.4643603/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            <  SRR4302535.3242230/94‑1 (MQ=60)
                                   CCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGC                                                            >  SRR4302535.4266903/1‑94 (MQ=60)
                                    CGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCG                                                           <  SRR4302535.233454/94‑1 (MQ=60)
                                    CGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCG                                                           >  SRR4302535.3003951/1‑94 (MQ=60)
                                    CGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCGCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCG                                                           <  SRR4302535.859951/94‑1 (MQ=60)
                                      TTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCT                                                         <  SRR4302535.2417173/94‑1 (MQ=60)
                                       TTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTG                                                        <  SRR4302535.847467/94‑1 (MQ=60)
                                         GCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGT                                                      <  SRR4302535.3234442/94‑1 (MQ=60)
                                          CGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     <  SRR4302535.3508760/94‑1 (MQ=60)
                                          CGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGGTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTG                                                     >  SRR4302535.2174733/1‑94 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 >  SRR4302535.373986/1‑94 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 <  SRR4302535.1148310/94‑1 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGGATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 >  SRR4302535.2645644/1‑94 (MQ=60)
                                              GTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGCAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCC                                                 >  SRR4302535.3831198/1‑94 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                >  SRR4302535.1723227/1‑94 (MQ=60)
                                               TACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCG                                                >  SRR4302535.1528720/1‑94 (MQ=60)
                                                ACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGT                                               >  SRR4302535.2279146/1‑94 (MQ=60)
                                                  TCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAA                                             >  SRR4302535.2196680/1‑94 (MQ=60)
                                                   CTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAG                                            >  SRR4302535.293567/1‑94 (MQ=60)
                                                    TTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGA                                           <  SRR4302535.2707127/94‑1 (MQ=60)
                                                      TTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAA                                         >  SRR4302535.1818192/1‑94 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCTAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       <  SRR4302535.3014569/94‑1 (MQ=60)
                                                        CCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAA                                       <  SRR4302535.3369256/94‑1 (MQ=60)
                                                         CTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAA                                      <  SRR4302535.995151/94‑1 (MQ=60)
                                                          TGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAG                                     <  SRR4302535.2397157/94‑1 (MQ=60)
                                                            GTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAA                                   <  SRR4302535.812753/94‑1 (MQ=60)
                                                            GTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAA                                   <  SRR4302535.2863561/94‑1 (MQ=60)
                                                            GTGGCTGCTCGGCGAGAATCCGGGGGCCGTGGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAA                                   <  SRR4302535.263755/94‑1 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAG                                  <  SRR4302535.759095/94‑1 (MQ=60)
                                                             TGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATGGTGCTGGAACTGGCGCTGGTGAGCCGTAAGAAAAAggaac                                  >  SRR4302535.1032148/1‑89 (MQ=60)
                                                              GGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGC                                 <  SRR4302535.2560419/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302535.4212/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302535.3803699/94‑1 (MQ=60)
                                                               GCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCC                                <  SRR4302535.3987945/94‑1 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGT                              >  SRR4302535.3241219/1‑94 (MQ=60)
                                                                 TGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGT                              >  SRR4302535.685597/1‑94 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGGCGTAAGAAAAAAGAAGCCGTCA                            >  SRR4302535.2316336/1‑94 (MQ=60)
                                                                   CTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCA                            <  SRR4302535.164061/94‑1 (MQ=60)
                                                                    TCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAG                           <  SRR4302535.3236188/94‑1 (MQ=60)
                                                                     CGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGT                          <  SRR4302535.296508/94‑1 (MQ=60)
                                                                      GGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTG                         <  SRR4302535.2676534/94‑1 (MQ=60)
                                                                       GCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302535.2123555/94‑1 (MQ=60)
                                                                       GCGAGAAGCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGT                        <  SRR4302535.3172298/94‑1 (MQ=60)
                                                                          AGAATCTGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTCGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAA                     <  SRR4302535.896668/94‑1 (MQ=60)
                                                                           GAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAA                    <  SRR4302535.2459240/94‑1 (MQ=60)
                                                                            AATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAA                   <  SRR4302535.4189232/94‑1 (MQ=60)
                                                                              TCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGG                 <  SRR4302535.2134142/94‑1 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               >  SRR4302535.4380429/1‑94 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               <  SRR4302535.2092135/94‑1 (MQ=60)
                                                                                CGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGAT               >  SRR4302535.4287745/1‑94 (MQ=60)
                                                                                 GGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATC              <  SRR4302535.1022221/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302535.4310683/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302535.1912671/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302535.3883168/94‑1 (MQ=60)
                                                                                  GGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCT             <  SRR4302535.3452444/94‑1 (MQ=60)
                                                                                    GGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGA           <  SRR4302535.2235185/94‑1 (MQ=60)
                                                                                     GCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAA          <  SRR4302535.1500504/94‑1 (MQ=60)
                                                                                      CCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAT         <  SRR4302535.4250856/94‑1 (MQ=60)
                                                                                       CGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATT        <  SRR4302535.2606010/94‑1 (MQ=60)
                                                                                         TTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT      <  SRR4302535.4338079/94‑1 (MQ=60)
                                                                                             GGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  <  SRR4302535.4158229/94‑1 (MQ=60)
                                                                                             GGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  <  SRR4302535.330976/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGCCAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCT  >  NC_000913/4134416‑4134602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑