Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,140,312 G→A M68I (ATG→ATA)  frwC → PTS system fructose‑like enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,140,3120GA100.0% 893.9 / NA 244M68I (ATG→ATA) frwCPTS system fructose‑like enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (134/110);  total (134/110)

Only 100 of 248 total aligned reads displayed.

GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401
                                                                                          |                                                                                         
gttgtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGt                                                                                         <  2:2324846/93‑1 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  2:597978/93‑1 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      >  1:3013365/1‑93 (MQ=255)
   gtATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGcc                                                                                      <  1:2993514/93‑1 (MQ=255)
    tATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCt                                                                                     <  2:1631731/93‑1 (MQ=255)
    tATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCt                                                                                     >  1:916107/1‑93 (MQ=255)
     aTGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCtt                                                                                    <  1:1985558/93‑1 (MQ=255)
         cAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCt                                                                                <  2:941282/93‑1 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              >  1:108680/1‑93 (MQ=255)
           aaGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCg                                                                              <  2:3708246/93‑1 (MQ=255)
            aGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGc                                                                             <  2:2116616/93‑1 (MQ=255)
            aGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGc                                                                             >  1:2130390/1‑93 (MQ=255)
             ggCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCa                                                                            >  1:3832432/1‑93 (MQ=255)
              gcgcAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAg                                                                           <  2:4549361/93‑1 (MQ=255)
                 cAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGt                                                                        >  1:2921085/1‑93 (MQ=255)
                  aGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGGGTa                                                                       >  2:3118992/1‑93 (MQ=255)
                   gTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAc                                                                      >  1:3680417/1‑93 (MQ=255)
                    tGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACa                                                                     >  1:2590486/1‑93 (MQ=255)
                      ccGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTTCATc                                                                   <  2:4379630/93‑1 (MQ=255)
                       cGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCg                                                                  >  1:4033820/1‑93 (MQ=255)
                        ggATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCgg                                                                 >  1:4533796/1‑93 (MQ=255)
                         gATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGt                                                                >  2:1069495/1‑93 (MQ=255)
                          aTGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGtt                                                               <  2:2873903/93‑1 (MQ=255)
                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                              >  2:1820631/1‑93 (MQ=255)
                           tGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTa                                                              >  2:2586415/1‑93 (MQ=255)
                            gCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAt                                                             <  1:3528928/93‑1 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        >  1:2692605/1‑93 (MQ=255)
                                 aGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAt                                                        <  1:3452105/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  2:564092/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       <  2:1014423/93‑1 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  1:2916654/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  2:872805/1‑93 (MQ=255)
                                  gCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAtt                                                       >  1:2402567/1‑93 (MQ=255)
                                   ccGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTg                                                      <  2:2149113/93‑1 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTTGCTATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     >  2:297540/1‑93 (MQ=255)
                                    cGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGc                                                     >  2:3241628/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:576038/1‑93 (MQ=255)
                                      aTCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCag                                                   >  1:17023/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:3355177/93‑1 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:3582240/93‑1 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  >  2:3553269/1‑93 (MQ=255)
                                       tCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCaga                                                  <  1:1512383/93‑1 (MQ=255)
                                          aaaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCg                                               >  1:4158356/1‑93 (MQ=255)
                                           aaTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGt                                              >  1:2251217/1‑93 (MQ=255)
                                            aTCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCACAGCGtt                                             >  2:1405068/1‑93 (MQ=255)
                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           >  2:2030054/1‑93 (MQ=255)
                                              cTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCt                                           <  2:2041926/93‑1 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  1:3722220/1‑93 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          <  1:1192379/93‑1 (MQ=255)
                                               tGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTg                                          >  1:1815928/1‑93 (MQ=255)
                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         >  2:2677261/1‑93 (MQ=255)
                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         <  1:3189281/93‑1 (MQ=255)
                                                gAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgc                                         <  2:1219967/93‑1 (MQ=255)
                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        >  1:1046371/1‑93 (MQ=255)
                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        >  1:2941810/1‑93 (MQ=255)
                                                 aaaaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATACTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTgcg                                        >  1:2509765/1‑93 (MQ=255)
                                                   aaaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCt                                      <  1:658934/93‑1 (MQ=255)
                                                    aaaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTg                                     >  1:177499/1‑93 (MQ=255)
                                                     aaCTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTgg                                    >  2:478959/1‑93 (MQ=255)
                                                        tGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCATCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTc                                 <  1:3295048/93‑1 (MQ=255)
                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  2:4462401/1‑93 (MQ=255)
                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  1:1054970/1‑93 (MQ=255)
                                                         gTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTcc                                >  1:447143/1‑93 (MQ=255)
                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               <  1:3883367/93‑1 (MQ=255)
                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               >  2:711126/1‑93 (MQ=255)
                                                          tttGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCg                               <  2:2489045/93‑1 (MQ=255)
                                                            tGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTg                             >  2:506460/1‑93 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  2:1728526/93‑1 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          >  2:4331344/1‑93 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  1:4296653/93‑1 (MQ=255)
                                                               tatCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTgcgc                          <  1:905478/93‑1 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:4246736/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  1:4451854/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:4164912/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:4407860/93‑1 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        >  2:3970193/1‑93 (MQ=255)
                                                                 tCGGCGTTGCGGGCTTGACGCTGATAGTGCCTCGCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTa                        <  2:2460838/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:4586952/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:2857717/93‑1 (MQ=255)
                                                                  cGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTAt                       <  1:2821994/93‑1 (MQ=255)
                                                                   ggCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATc                      <  1:3575701/93‑1 (MQ=255)
                                                                     cGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCgg                    >  1:2344820/1‑93 (MQ=255)
                                                                     cGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCgg                    <  2:1950253/93‑1 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  1:2463943/1‑93 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  2:1410620/1‑93 (MQ=255)
                                                                      gTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGt                   >  2:284132/1‑93 (MQ=255)
                                                                         gCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGcc                <  1:1960004/93‑1 (MQ=255)
                                                                           gggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTg              <  1:138485/93‑1 (MQ=255)
                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             >  2:4056116/1‑93 (MQ=255)
                                                                            ggCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTgg             <  1:146567/93‑1 (MQ=255)
                                                                             gCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTggg            >  2:4640814/1‑93 (MQ=255)
                                                                               ttGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGtt          <  2:1198296/93‑1 (MQ=255)
                                                                                 gACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTgg        <  1:323917/93‑1 (MQ=255)
                                                                                   cGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGTGTTGGTa      >  2:2347936/1‑93 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  1:3916208/1‑93 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  2:668062/1‑93 (MQ=255)
                                                                                    gCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTaa     >  1:940729/1‑93 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    <  2:342964/93‑1 (MQ=255)
                                                                                     cTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAAc    >  2:3291848/1‑93 (MQ=255)
                                                                                      tGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACa   <  1:1707500/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG  >  NC_000913/4140222‑4140401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC  >  NC_000913/4140219‑4140402
                                                                                             |                                                                                          
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATA                                                                                            <  SRR4302535.4385714/94‑1 (MQ=60)
 ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                           <  SRR4302535.1053489/94‑1 (MQ=60)
 ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                           >  SRR4302535.292905/1‑94 (MQ=60)
 ATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAG                                                                                           >  SRR4302535.4561921/1‑94 (MQ=60)
  TGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGT                                                                                          <  SRR4302535.2681835/94‑1 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                         >  SRR4302535.2946340/1‑94 (MQ=60)
   GTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTG                                                                                         <  SRR4302535.4563761/94‑1 (MQ=60)
    TTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGC                                                                                        <  SRR4302535.2815871/94‑1 (MQ=60)
    TTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGC                                                                                        >  SRR4302535.141973/1‑94 (MQ=60)
     TGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCC                                                                                       >  SRR4302535.3034481/1‑94 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                      <  SRR4302535.3014503/94‑1 (MQ=60)
      GTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCT                                                                                      >  SRR4302535.922609/1‑94 (MQ=60)
        ATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTT                                                                                    <  SRR4302535.1999855/94‑1 (MQ=60)
         TGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTC                                                                                   >  SRR4302535.3375685/1‑94 (MQ=60)
          GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                  <  SRR4302535.226500/94‑1 (MQ=60)
          GGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCC                                                                                  <  SRR4302535.4590966/94‑1 (MQ=60)
            CAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTC                                                                                <  SRR4302535.1586264/94‑1 (MQ=60)
             AAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCG                                                                               >  SRR4302535.109447/1‑94 (MQ=60)
              AAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGC                                                                              >  SRR4302535.2145749/1‑94 (MQ=60)
              AAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGC                                                                              >  SRR4302535.2145748/1‑94 (MQ=60)
               AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                             >  SRR4302535.3858861/1‑94 (MQ=60)
               AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                             >  SRR4302535.802611/1‑94 (MQ=60)
               AGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCA                                                                             <  SRR4302535.1368526/94‑1 (MQ=60)
                  CGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCG                                                                          >  SRR4302535.3288803/1‑94 (MQ=60)
                   GCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGT                                                                         >  SRR4302535.3566314/1‑94 (MQ=60)
                   GCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGT                                                                         >  SRR4302535.2941693/1‑94 (MQ=60)
                     AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                       >  SRR4302535.3545639/1‑94 (MQ=60)
                     AGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTAC                                                                       >  SRR4302535.3705889/1‑94 (MQ=60)
                      GTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACA                                                                      >  SRR4302535.2609087/1‑94 (MQ=60)
                       TGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACAT                                                                     <  SRR4302535.171698/94‑1 (MQ=60)
                         CCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCG                                                                   >  SRR4302535.4061421/1‑94 (MQ=60)
                          CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                  >  SRR4302535.4564823/1‑94 (MQ=60)
                          CGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGG                                                                  >  SRR4302535.2428048/1‑94 (MQ=60)
                            GATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTT                                                                <  SRR4302535.3415492/94‑1 (MQ=60)
                               GCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATT                                                             <  SRR4302535.3553434/94‑1 (MQ=60)
                                CCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTC                                                            >  SRR4302535.2737589/1‑94 (MQ=60)
                                   TAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCAT                                                         >  SRR4302535.2711852/1‑94 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                        <  SRR4302535.3476099/94‑1 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                        <  SRR4302535.1788863/94‑1 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                        >  SRR4302535.2937245/1‑94 (MQ=60)
                                    AGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATT                                                        >  SRR4302535.2419849/1‑94 (MQ=60)
                                     GCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTG                                                       >  SRR4302535.1001757/1‑94 (MQ=60)
                                      CCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGC                                                      >  SRR4302535.3917459/1‑94 (MQ=60)
                                       CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                     >  SRR4302535.4336048/1‑94 (MQ=60)
                                       CGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCA                                                     >  SRR4302535.1094943/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                    >  SRR4302535.580085/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                    >  SRR4302535.1490725/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                    >  SRR4302535.17150/1‑94 (MQ=60)
                                        GATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAG                                                    <  SRR4302535.4408181/94‑1 (MQ=60)
                                         ATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGA                                                   >  SRR4302535.557611/1‑94 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                  <  SRR4302535.3607087/94‑1 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                  >  SRR4302535.3818237/1‑94 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                  <  SRR4302535.1523277/94‑1 (MQ=60)
                                          TCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAG                                                  <  SRR4302535.3378538/94‑1 (MQ=60)
                                           CCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGC                                                 >  SRR4302535.2865921/1‑94 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                <  SRR4302535.3602752/94‑1 (MQ=60)
                                            CAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCG                                                >  SRR4302535.4186821/1‑94 (MQ=60)
                                             AAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGT                                               >  SRR4302535.2267249/1‑94 (MQ=60)
                                              AATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTT                                              >  SRR4302535.1922015/1‑94 (MQ=60)
                                              AATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTT                                              <  SRR4302535.1085552/94‑1 (MQ=60)
                                               ATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTC                                             >  SRR4302535.1779104/1‑94 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                            <  SRR4302535.643152/94‑1 (MQ=60)
                                                TCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCT                                            <  SRR4302535.4127950/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                           >  SRR4302535.1829056/1‑94 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                           >  SRR4302535.3748006/1‑94 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                           >  SRR4302535.4276922/1‑94 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                           <  SRR4302535.1180483/94‑1 (MQ=60)
                                                 CTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTG                                           >  SRR4302535.1999866/1‑94 (MQ=60)
                                                  TGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGC                                          <  SRR4302535.1200919/94‑1 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                         <  SRR4302535.3211335/94‑1 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                         >  SRR4302535.3013730/1‑94 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                         >  SRR4302535.1053788/1‑94 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                         >  SRR4302535.2962498/1‑94 (MQ=60)
                                                   GAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATACTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCG                                         >  SRR4302535.2527858/1‑94 (MQ=60)
                                                    AAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCATCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGC                                        >  SRR4302535.2413461/1‑94 (MQ=60)
                                                    AAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGC                                        >  SRR4302535.288725/1‑94 (MQ=60)
                                                     AAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCT                                       >  SRR4302535.2941257/1‑94 (MQ=60)
                                                      AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                      <  SRR4302535.663532/94‑1 (MQ=60)
                                                      AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                      >  SRR4302535.2702261/1‑94 (MQ=60)
                                                      AAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTG                                      >  SRR4302535.178732/1‑94 (MQ=60)
                                                        AACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGC                                    <  SRR4302535.2355726/94‑1 (MQ=60)
                                                         ACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCT                                   >  SRR4302535.1885048/1‑94 (MQ=60)
                                                         ACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCT                                   >  SRR4302535.3435474/1‑94 (MQ=60)
                                                          CTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTC                                  >  SRR4302535.2575293/1‑94 (MQ=60)
                                                           TGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCATCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCC                                 <  SRR4302535.3317954/94‑1 (MQ=60)
                                                           TGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCC                                 >  SRR4302535.450275/1‑94 (MQ=60)
                                                           TGTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCC                                 >  SRR4302535.1062472/1‑94 (MQ=60)
                                                            GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                <  SRR4302535.1769941/94‑1 (MQ=60)
                                                            GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                <  SRR4302535.1713500/94‑1 (MQ=60)
                                                            GTTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCG                                >  SRR4302535.3172037/1‑94 (MQ=60)
                                                             TTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGT                               <  SRR4302535.403946/94‑1 (MQ=60)
                                                             TTTGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGT                               <  SRR4302535.3910008/94‑1 (MQ=60)
                                                               TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                             >  SRR4302535.2274488/1‑94 (MQ=60)
                                                               TGATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGC                             >  SRR4302535.4482609/1‑94 (MQ=60)
                                                                GATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGAGTTCTGCGCTGGCTCCGTGCG                            >  SRR4302535.739310/1‑94 (MQ=60)
                                                                 ATATCTGCGTTGCGGTCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                           <  SRR4302535.2441477/94‑1 (MQ=60)
                                                                 ATATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGC                           <  SRR4302535.3592589/94‑1 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                          <  SRR4302535.4326057/94‑1 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                          <  SRR4302535.3146713/94‑1 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                          <  SRR4302535.911903/94‑1 (MQ=60)
                                                                  TATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCT                          <  SRR4302535.3913001/94‑1 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                         >  SRR4302535.1983417/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                         >  SRR4302535.4482384/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                         >  SRR4302535.3273373/1‑94 (MQ=60)
                                                                   ATCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                         >  SRR4302535.3619948/1‑94 (MQ=60)
                                                                   AGCGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTA                         <  SRR4302535.53044/94‑1 (MQ=60)
                                                                     CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                       <  SRR4302535.2842031/94‑1 (MQ=60)
                                                                     CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                       <  SRR4302535.4618188/94‑1 (MQ=60)
                                                                     CGGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATC                       <  SRR4302535.2877961/94‑1 (MQ=60)
                                                                      GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                      <  SRR4302535.3600490/94‑1 (MQ=60)
                                                                      GGCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCG                      >  SRR4302535.2505355/1‑94 (MQ=60)
                                                                       GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                     >  SRR4302535.2361621/1‑94 (MQ=60)
                                                                       GCGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGG                     <  SRR4302535.1055195/94‑1 (MQ=60)
                                                                        CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                    >  SRR4302535.2481708/1‑94 (MQ=60)
                                                                        CGTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGT                    >  SRR4302535.3243118/1‑94 (MQ=60)
                                                                         GTTGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTG                   >  SRR4302535.2959238/1‑94 (MQ=60)
                                                                           TGCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCC                 <  SRR4302535.3908245/94‑1 (MQ=60)
                                                                            GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                <  SRR4302535.1974141/94‑1 (MQ=60)
                                                                            GCGGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCT                <  SRR4302535.1401394/94‑1 (MQ=60)
                                                                              GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG              >  SRR4302535.920591/1‑94 (MQ=60)
                                                                              GGGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGG              <  SRR4302535.139454/94‑1 (MQ=60)
                                                                               GGCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGG             <  SRR4302535.147587/94‑1 (MQ=60)
                                                                                GCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGT            <  SRR4302535.3020785/94‑1 (MQ=60)
                                                                                GCTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTAATCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGT            >  SRR4302535.2533993/1‑94 (MQ=60)
                                                                                 CTTGACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTT           >  SRR4302535.4063483/1‑94 (MQ=60)
                                                                                    GACGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGT        <  SRR4302535.326153/94‑1 (MQ=60)
                                                                                      CGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAA      >  SRR4302535.947386/1‑94 (MQ=60)
                                                                                      CGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAA      >  SRR4302535.3943007/1‑94 (MQ=60)
                                                                                      CGCTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAA      >  SRR4302535.3708332/1‑94 (MQ=60)
                                                                                        CTGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACA    <  SRR4302535.926574/94‑1 (MQ=60)
                                                                                         TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG   <  SRR4302535.1719845/94‑1 (MQ=60)
                                                                                         TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG   >  SRR4302535.455088/1‑94 (MQ=60)
                                                                                         TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG   <  SRR4302535.3805071/94‑1 (MQ=60)
                                                                                         TGATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAG   <  SRR4302535.3610053/94‑1 (MQ=60)
                                                                                          GATAGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC  <  SRR4302535.2900719/94‑1 (MQ=60)
                                                                                             |                                                                                          
CATGTTGTATGGCAAAGGCGCAGTGCCGGATGCCGTAGCCGATCCAAATCTGAAAAAACTGTTTGATATCGGCGTTGCGGGCTTGACGCTGATGGTGCCTTTCCTCGCAGCGTACATCGGTTATTCCATTGCAGAGCGTTCTGCGCTGGCTCCGTGCGCTATCGGTGCCTGGGTTGGTAACAGC  >  NC_000913/4140219‑4140402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑