Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,566	A→G	G515G (GGT→GGC)	ydeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,566	0	A	G	99.6%	752.3 / ‑5.2	237	G515G (GGT→GGC)	ydeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (105/130); total (107/130)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 244 total aligned reads displayed.

ATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCA  >  NC_000913/1594476‑1594654

aTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGcc                                                                                        >  1:6796358/1‑93 (MQ=255)

 ttCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCa                                                                                       >  2:6482881/1‑93 (MQ=255)

   ccAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCAtt                                                                                     >  2:643069/1‑93 (MQ=255)

   ccAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCAtt                                                                                     <  1:1251708/93‑1 (MQ=255)

    cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTa                                                                                    >  1:722752/1‑93 (MQ=255)

     agagACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTAc                                                                                   >  1:1857168/1‑93 (MQ=255)

      gagaCTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACt                                                                                  >  1:1106705/1‑93 (MQ=255)

          cTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACa                                                                              <  1:1719002/93‑1 (MQ=255)

          cTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACa                                                                              >  2:883326/1‑93 (MQ=255)

          cTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACa                                                                              >  1:2754632/1‑93 (MQ=255)

          cTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACa                                                                              >  2:1996322/1‑93 (MQ=255)

           tGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAt                                                                             >  1:9223662/1‑93 (MQ=255)

           tGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAt                                                                             >  1:4557041/1‑93 (MQ=255)

            gTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                                                                            >  2:8247321/1‑93 (MQ=255)

            gTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                                                                            >  2:782220/1‑93 (MQ=255)

            gTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                                                                            <  1:6289524/93‑1 (MQ=255)

            gTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                                                                            >  2:5006025/1‑93 (MQ=255)

            gTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                                                                            >  2:7168620/1‑93 (MQ=255)

              ccGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTa                                                                          <  1:4035488/93‑1 (MQ=255)

              ccGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTa                                                                          <  2:5683701/93‑1 (MQ=255)

               cGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGCTAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtttcc                                                                         >  1:9071348/1‑91 (MQ=255)

               cGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTAc                                                                         <  2:8673850/93‑1 (MQ=255)

                gTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACg                                                                        >  1:8521199/1‑93 (MQ=255)

                 ttGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGa                                                                       >  1:4489336/1‑93 (MQ=255)

                 ttGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGa                                                                       <  2:1715727/93‑1 (MQ=255)

                  tGAAATATTAACTATCCCCTTACCGGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGa                                                                       <  1:5096257/93‑1 (MQ=255)

                  tGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGAt                                                                      >  1:9325485/1‑93 (MQ=255)

                   gAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATa                                                                     >  2:2726253/1‑93 (MQ=255)

                    aaaTATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAc                                                                    <  2:9204197/93‑1 (MQ=255)

                    aaaTATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAc                                                                    <  1:1903368/93‑1 (MQ=255)

                    aaaTATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAc                                                                    <  2:9920/93‑1 (MQ=255)

                       tatTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGc                                                                 <  1:2353961/93‑1 (MQ=255)

                        atTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCt                                                                <  2:1353281/93‑1 (MQ=255)

                        atTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGCTGGGGCCATTACTGACATTTACGATACAGCt                                                                >  1:96625/1‑93 (MQ=255)

                         ttAACTATCCCCTTACC‑GGGGGCCTTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTa                                                               <  2:3506518/93‑1 (MQ=255)

                          tAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTAc                                                              <  1:1365963/93‑1 (MQ=255)

                              tATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa                                                          >  1:7684956/1‑93 (MQ=255)

                              tATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa                                                          >  1:1321642/1‑93 (MQ=255)

                              tATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa                                                          >  2:9023413/1‑93 (MQ=255)

                              tATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa                                                          >  1:5476436/1‑93 (MQ=255)

                               aTCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaaa                                                         <  1:1187646/93‑1 (MQ=255)

                               aTCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaaa                                                         >  1:8493932/1‑93 (MQ=255)

                                tCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAAt                                                        >  2:3367325/1‑93 (MQ=255)

                                 ccccTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAAtt                                                       <  1:7897416/93‑1 (MQ=255)

                                    cTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTAcc                                                    <  2:7086861/93‑1 (MQ=255)

                                    cTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTAcc                                                    >  2:8355774/1‑93 (MQ=255)

                                     ttACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCg                                                   >  2:4538117/1‑93 (MQ=255)

                                     ttACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCg                                                   >  2:36120/1‑93 (MQ=255)

                                       aCC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc                                                 >  2:2258755/1‑93 (MQ=255)

                                        cc‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACa                                                <  1:5897583/93‑1 (MQ=255)

                                         c‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAt                                               <  2:2196681/93‑1 (MQ=255)

                                           gggggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                              >  2:4090610/4‑93 (MQ=255)

                                            ggggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTg                                             >  1:3680472/3‑93 (MQ=255)

                                            ggggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTg                                             >  1:4967753/3‑93 (MQ=255)

                                            ggggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTg                                             >  1:7463353/3‑93 (MQ=255)

                                             gggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGa                                            <  1:9894824/92‑1 (MQ=255)

                                              ggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGAt                                           >  2:2455260/1‑93 (MQ=255)

                                               gCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATa                                          <  1:43037/93‑1 (MQ=255)

                                                cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                         >  2:5757268/1‑93 (MQ=255)

                                                cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                         <  1:8850835/93‑1 (MQ=255)

                                                  tttCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAccc                                       >  2:3777583/1‑93 (MQ=255)

                                                  tttCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAccc                                       >  2:4009693/1‑93 (MQ=255)

                                                   ttCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCca                                      >  1:1547922/1‑93 (MQ=255)

                                                      ctccGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAg                                   >  2:673677/1‑93 (MQ=255)

                                                      ctccGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAg                                   <  2:1795065/93‑1 (MQ=255)

                                                      ctccGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAg                                   >  1:3280163/1‑93 (MQ=255)

                                                       tccGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGt                                  >  1:5264604/1‑93 (MQ=255)

                                                        ccGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTa                                 >  2:192751/1‑93 (MQ=255)

                                                        ccGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTa                                 <  2:5409024/93‑1 (MQ=255)

                                                          gATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATc                               <  2:1466889/93‑1 (MQ=255)

                                                           aTAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCg                              >  1:5721076/1‑93 (MQ=255)

                                                               aTCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                          >  1:9543311/1‑93 (MQ=255)

                                                                tCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACga                         <  1:6630444/93‑1 (MQ=255)

                                                                tCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACga                         >  1:4710742/1‑93 (MQ=255)

                                                                tCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACga                         <  1:3918350/93‑1 (MQ=255)

                                                                tCCATAGCCCGTTGAATTAAGGGAGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACCGTATCGAACga                         >  2:7556559/1‑93 (MQ=255)

                                                                 ccATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgat                        >  2:4377337/1‑93 (MQ=255)

                                                                  cATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatg                       <  2:8373112/93‑1 (MQ=255)

                                                                   aTAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatga                      <  2:412100/93‑1 (MQ=255)

                                                                   aTAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatga                      <  2:9409247/93‑1 (MQ=255)

                                                                   aTAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatga                      <  1:7746927/93‑1 (MQ=255)

                                                                   aTAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatga                      <  2:9845675/93‑1 (MQ=255)

                                                                    tAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgatgat                     >  1:3104529/1‑93 (MQ=255)

                                                                       cccGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTc                  <  1:115346/93‑1 (MQ=255)

                                                                       cccGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTc                  <  1:5996492/93‑1 (MQ=255)

                                                                         cGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGc                >  2:687649/1‑93 (MQ=255)

                                                                         cGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGc                <  2:6131045/93‑1 (MQ=255)

                                                                             gAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcc            >  2:2723339/1‑93 (MQ=255)

                                                                              aaTTAATGGTGGCTCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAAGATGATGTCGcatcca           >  2:4761927/1‑93 (MQ=255)

                                                                                ttAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatccatc         >  2:6654745/1‑93 (MQ=255)

                                                                                 tAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa        <  2:5949823/93‑1 (MQ=255)

                                                                                 tAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa        <  2:3250029/93‑1 (MQ=255)

                                                                                 tAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCa        <  2:1219281/93‑1 (MQ=255)

                                                                                 tAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGACGATGTCGCATCCATCa        <  2:9555798/93‑1 (MQ=255)

                                                                                   aGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAgg      <  2:4145076/93‑1 (MQ=255)

                                                                                   aGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAgg      >  1:3810925/1‑93 (MQ=255)

                                                                                    gggTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGt     <  1:2179073/93‑1 (MQ=255)

                                                                                      gTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc   <  1:9737298/93‑1 (MQ=255)

                                                                                      gTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc   <  1:8612642/93‑1 (MQ=255)

                                                                                      gTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc   <  1:8148695/93‑1 (MQ=255)

ATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCA  >  NC_000913/1594476‑1594654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTG  >  NC_000913/1594473‑1594659

TAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCG                                                                                               <  SRR4302540.9994905/94‑1 (MQ=60)

TAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCG                                                                                               <  SRR4302540.9595655/94‑1 (MQ=60)

 AAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGCATTAAGGGTGGCGC                                                                                              >  SRR4302540.4657237/1‑94 (MQ=60)

 AAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGC                                                                                              <  SRR4302540.1622675/94‑1 (MQ=60)

  AATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCC                                                                                             >  SRR4302540.6871529/1‑94 (MQ=60)

  AATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCC                                                                                             <  SRR4302540.5665788/94‑1 (MQ=60)

      CCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTA                                                                                         >  SRR4302540.730497/1‑94 (MQ=60)

      CCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTA                                                                                         <  SRR4302540.1264489/94‑1 (MQ=60)

       CAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTAC                                                                                        >  SRR4302540.1877530/1‑94 (MQ=60)

        AGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACT                                                                                       <  SRR4302540.7976149/94‑1 (MQ=60)

        AGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACT                                                                                       >  SRR4302540.1118130/1‑94 (MQ=60)

         GAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTG                                                                                      <  SRR4302540.7758853/94‑1 (MQ=60)

          AGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGA                                                                                     <  SRR4302540.9622481/94‑1 (MQ=60)

           GACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGAA                                                                                    >  SRR4302540.5032196/1‑94 (MQ=60)

           GACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGAC                                                                                    >  SRR4302540.6117484/1‑94 (MQ=60)

            ACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACA                                                                                   >  SRR4302540.2784404/1‑94 (MQ=60)

             CTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAT                                                                                  >  SRR4302540.4606304/1‑94 (MQ=60)

             CTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAT                                                                                  >  SRR4302540.9334266/1‑94 (MQ=60)

             CTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAT                                                                                  <  SRR4302540.4505116/94‑1 (MQ=60)

             CTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAT                                                                                  <  SRR4302540.1737559/94‑1 (MQ=60)

               GTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTT                                                                                <  SRR4302540.6359532/94‑1 (MQ=60)

               GTCCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTT                                                                                >  SRR4302540.9075237/1‑94 (MQ=60)

                 CCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGCTAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTCC                                                                              >  SRR4302540.9180289/1‑94 (MQ=60)

                 CCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTAC                                                                              <  SRR4302540.5320074/94‑1 (MQ=60)

                 CCGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTAC                                                                              <  SRR4302540.4079002/94‑1 (MQ=60)

                  CGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACG                                                                             >  SRR4302540.8620223/1‑94 (MQ=60)

                  CGTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACG                                                                             <  SRR4302540.6504597/94‑1 (MQ=60)

                   GTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGA                                                                            >  SRR4302540.1766314/1‑94 (MQ=60)

                   GTTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGA                                                                            >  SRR4302540.4538020/1‑94 (MQ=60)

                    TTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGAT                                                                           <  SRR4302540.2629357/94‑1 (MQ=60)

                    TTGAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGAT                                                                           >  SRR4302540.9436716/1‑94 (MQ=60)

                     TGAAATATTAACTATCCCCTTACCGGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGAT                                                                           <  SRR4302540.5151402/94‑1 (MQ=60)

                      GAAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAC                                                                         <  SRR4302540.3976562/94‑1 (MQ=60)

                      GAAATATTAAC‑ATCCCCTTACC‑CGGGGCATTTTCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAAC                                                                        >  SRR4302540.2122857/1‑94 (MQ=60)

                       AAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACC                                                                        <  SRR4302540.1924178/94‑1 (MQ=60)

                       AAATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAACCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACC                                                                        >  SRR4302540.2126181/1‑94 (MQ=60)

                         ATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGC                                                                      <  SRR4302540.6321411/94‑1 (MQ=60)

                         ATATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGGTGAATTAAGGGAGGCGCCATTACTGACATTTACGATACCGC                                                                      >  SRR4302540.3303707/1‑94 (MQ=60)

                          TATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCT                                                                     <  SRR4302540.2379314/94‑1 (MQ=60)

                          TATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGCTGGGGCCATTACTGACATTTACGATACAGCT                                                                     >  SRR4302540.97649/1‑94 (MQ=60)

                           ATTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTA                                                                    >  SRR4302540.9668804/1‑94 (MQ=60)

                            TTAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTAC                                                                   >  SRR4302540.870229/1‑94 (MQ=60)

                             TAACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACC                                                                  <  SRR4302540.1380415/94‑1 (MQ=60)

                              AACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTTAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCA                                                                 <  SRR4302540.8131322/94‑1 (MQ=60)

                              AACTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCA                                                                 <  SRR4302540.127494/94‑1 (MQ=60)

                                CTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAA                                                               >  SRR4302540.1335412/1‑94 (MQ=60)

                                CTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAA                                                               >  SRR4302540.7772100/1‑94 (MQ=60)

                                CTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAA                                                               >  SRR4302540.5536713/1‑94 (MQ=60)

                                CTATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATGACTGACATTTACGATCCCGCTACCAAA                                                               >  SRR4302540.5536712/1‑94 (MQ=60)

                                 TATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGATATTTACGATACCGCTACCAAAA                                                              >  SRR4302540.4597447/1‑94 (MQ=60)

                                 TATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAA                                                              >  SRR4302540.1246643/1‑94 (MQ=60)

                                 TATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAA                                                              >  SRR4302540.8592632/1‑94 (MQ=60)

                                  ATCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAAT                                                             <  SRR4302540.1199891/94‑1 (MQ=60)

                                   TCCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATT                                                            <  SRR4302540.4691692/94‑1 (MQ=60)

                                    CCCCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTA                                                           <  SRR4302540.7989549/94‑1 (MQ=60)

                                      CCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACC                                                         <  SRR4302540.8615801/94‑1 (MQ=60)

                                      CCTTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACC                                                         >  SRR4302540.4083030/1‑94 (MQ=60)

                                        TTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGA                                                       >  SRR4302540.6716733/1‑94 (MQ=60)

                                        TTACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGA                                                       <  SRR4302540.8540159/94‑1 (MQ=60)

                                         TCCC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGCGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGAC                                                      <  SRR4302540.3035239/94‑1 (MQ=60)

                                         TACC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGAC                                                      <  SRR4302540.2350249/94‑1 (MQ=60)

                                           CC‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAT                                                    <  SRR4302540.5962714/94‑1 (MQ=60)

                                            cccggGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAT                                                    <  SRR4302540.5628969/89‑1 (MQ=60)

                                            C‑GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATT                                                   <  SRR4302540.4768809/94‑1 (MQ=60)

                                             cgggcGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATT                                                   <  SRR4302540.4768808/89‑1 (MQ=60)

                                              GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTTCCAAAATTACCGACATTG                                                  <  SRR4302540.324418/94‑1 (MQ=60)

                                              GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTG                                                  >  SRR4302540.5021494/1‑94 (MQ=60)

                                              GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTG                                                  >  SRR4302540.7546579/1‑94 (MQ=60)

                                              GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTG                                                  >  SRR4302540.7546581/1‑94 (MQ=60)

                                              GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTG                                                  <  SRR4302540.7636030/94‑1 (MQ=60)

                                              GGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTG                                                  >  SRR4302540.3720698/1‑94 (MQ=60)

                                                GGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGAT                                                >  SRR4302540.9274881/1‑94 (MQ=60)

                                                GGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGAT                                                <  SRR4302540.10010374/94‑1 (MQ=60)

                                                  GCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAC                                              <  SRR4302540.43479/94‑1 (MQ=60)

                                                   CATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACC                                             <  SRR4302540.4155367/94‑1 (MQ=60)

                                                   CATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACC                                             <  SRR4302540.5155156/94‑1 (MQ=60)

                                                   CATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACC                                             <  SRR4302540.8953227/94‑1 (MQ=60)

                                                   CATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACC                                             <  SRR4302540.8953226/94‑1 (MQ=60)

                                                    ATTTCCTCCGATAAATCCATAGCCCGTTGCATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCC                                            >  SRR4302540.1253848/1‑94 (MQ=60)

                                                    ATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCC                                            >  SRR4302540.5909819/1‑94 (MQ=60)

                                                    ATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCC                                            >  SRR4302540.7207298/1‑94 (MQ=60)

                                                     TTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCA                                           <  SRR4302540.5949069/94‑1 (MQ=60)

                                                     TTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCA                                           <  SRR4302540.9352320/94‑1 (MQ=60)

                                                     TTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCA                                           >  SRR4302540.1564993/1‑94 (MQ=60)

                                                      TTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCAC                                          <  SRR4302540.1720187/94‑1 (MQ=60)

                                                        CCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAG                                        >  SRR4302540.3315992/1‑94 (MQ=60)

                                                         CTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGT                                       >  SRR4302540.5322055/1‑94 (MQ=60)

                                                         CTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGT                                       <  SRR4302540.6007630/94‑1 (MQ=60)

                                                            CGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATC                                    <  SRR4302540.7811165/94‑1 (MQ=60)

                                                            CGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATC                                    >  SRR4302540.4590723/1‑94 (MQ=60)

                                                             GATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCG                                   >  SRR4302540.9777865/1‑94 (MQ=60)

                                                             GATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCG                                   >  SRR4302540.5784050/1‑94 (MQ=60)

                                                              ATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGA                                  >  SRR4302540.4809316/1‑94 (MQ=60)

                                                                AAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAC                                <  SRR4302540.190855/94‑1 (MQ=60)

                                                                 AATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACG                               >  SRR4302540.2471550/1‑94 (MQ=60)

                                                                 AATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACG                               >  SRR4302540.9656233/1‑94 (MQ=60)

                                                                 AATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACG                               <  SRR4302540.3347332/94‑1 (MQ=60)

                                                                  ATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGA                              >  SRR4302540.4761086/1‑94 (MQ=60)

                                                                  ATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGA                              >  SRR4302540.9192746/1‑94 (MQ=60)

                                                                   TCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGAT                             <  SRR4302540.6703839/94‑1 (MQ=60)

                                                                   TCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGAT                             <  SRR4302540.3960773/94‑1 (MQ=60)

                                                                    CCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATG                            <  SRR4302540.9437179/94‑1 (MQ=60)

                                                                     CATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGA                           <  SRR4302540.3866232/94‑1 (MQ=60)

                                                                      ATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGAT                          <  SRR4302540.7837383/94‑1 (MQ=60)

                                                                      ATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGAT                          >  SRR4302540.3138521/1‑94 (MQ=60)

                                                                      ATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGAT                          <  SRR4302540.1147482/94‑1 (MQ=60)

                                                                         GCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTC                       <  SRR4302540.1338679/94‑1 (MQ=60)

                                                                         GCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTC                       <  SRR4302540.1338680/94‑1 (MQ=60)

                                                                          CCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCG                      <  SRR4302540.6062939/94‑1 (MQ=60)

                                                                          CCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCG                      <  SRR4302540.116576/94‑1 (MQ=60)

                                                                          CCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCG                      <  SRR4302540.10000701/94‑1 (MQ=60)

                                                                          CCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCG                      <  SRR4302540.8458396/94‑1 (MQ=60)

                                                                             GTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCAT                   >  SRR4302540.1183433/1‑94 (MQ=60)

                                                                                 AATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCAT               <  SRR4302540.3836379/94‑1 (MQ=60)

                                                                                 AATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCAT               >  SRR4302540.9843041/1‑94 (MQ=60)

                                                                                  ATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATC              >  SRR4302540.3721662/1‑94 (MQ=60)

                                                                                  ATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATC              >  SRR4302540.7920541/1‑94 (MQ=60)

                                                                                   TTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCA             <  SRR4302540.8646978/94‑1 (MQ=60)

                                                                                    TAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAG            <  SRR4302540.1681394/94‑1 (MQ=60)

                                                                                    TAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAG            <  SRR4302540.1392381/94‑1 (MQ=60)

                                                                                     AAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGG           >  SRR4302540.3852423/1‑94 (MQ=60)

                                                                                      AGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGT          >  SRR4302540.5580716/1‑94 (MQ=60)

                                                                                       GGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTT         <  SRR4302540.2202575/94‑1 (MQ=60)

                                                                                         GTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCA       <  SRR4302540.8243288/94‑1 (MQ=60)

                                                                                         GTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCA       <  SRR4302540.9851615/94‑1 (MQ=60)

                                                                                         GTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCA       <  SRR4302540.8712714/94‑1 (MQ=60)

                                                                                          TGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAG      <  SRR4302540.6786915/94‑1 (MQ=60)

                                                                                             CGCCATTACTGACATTTACGATACCTCTACCAAAATTACCGACATTGACACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGT   <  SRR4302540.4654391/94‑1 (MQ=60)

                                                                                             CGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGT   <  SRR4302540.7520326/94‑1 (MQ=60)

                                                                                             CGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGT   <  SRR4302540.7187604/94‑1 (MQ=60)

                                                                                             CGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGT   >  SRR4302540.2160738/1‑94 (MQ=60)

                                                                                              GCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTG  <  SRR4302540.750173/94‑1 (MQ=60)

TAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACC‑GGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTG  >  NC_000913/1594473‑1594659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`