Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,927	T→C	L113L (TTA→TTG)	ydeW ←	putative transcriptional regulator, sorC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,927	0	T	C	99.6%	823.4 / NA	251	L113L (TTA→TTG)	ydeW	putative transcriptional regulator, sorC family
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (127/123); minor base A (1/0); total (128/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

TTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAG  >  NC_000913/1598837‑1599016

 tGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCaaa                                                                                        >  2:1154061/1‑93 (MQ=255)

 tGCTGTAACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCaaa                                                                                        >  2:6566013/1‑93 (MQ=255)

  gCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAAc                                                                                       >  1:1910140/1‑93 (MQ=255)

  gCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAAc                                                                                       <  2:4032621/93‑1 (MQ=255)

    tgtgACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACtc                                                                                     <  2:5981593/93‑1 (MQ=255)

    tgtgACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATAGCCAGCATCTGTTGTGGTTGAAGCAAACtc                                                                                     >  1:5992729/1‑93 (MQ=255)

     gtgACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACtca                                                                                    >  2:6113287/1‑93 (MQ=255)

     gtgACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACtca                                                                                    <  1:704715/93‑1 (MQ=255)

     gtgACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACtca                                                                                    >  2:6373623/1‑93 (MQ=255)

       gACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACtcatc                                                                                  >  2:8774220/1‑93 (MQ=255)

        aCGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACacatca                                                                                 <  2:481141/93‑1 (MQ=255)

         cGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCaa                                                                                <  2:4906258/93‑1 (MQ=255)

          gAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAAc                                                                               >  1:4073375/1‑93 (MQ=255)

           aaaTAAAACCACTTAAGCGTTGCAGCGTATCCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACa                                                                              <  2:9821506/93‑1 (MQ=255)

           aaaTAAAACCACTTAAGCGTTGCAGCGTACTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACa                                                                              <  2:73962/93‑1 (MQ=255)

            aaTAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACat                                                                             >  2:7911445/1‑93 (MQ=255)

            aaTAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACat                                                                             >  2:9382514/1‑93 (MQ=255)

             aTAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACgta                                                                            >  1:8126326/1‑93 (MQ=255)

             aTAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACata                                                                            <  2:2103251/93‑1 (MQ=255)

             aTAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACata                                                                            <  2:7537752/93‑1 (MQ=255)

             aTAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACata                                                                            >  1:6210952/1‑93 (MQ=255)

              tAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACatat                                                                           >  1:8549426/1‑93 (MQ=255)

              tAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACatat                                                                           >  2:4714124/1‑93 (MQ=255)

              tAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACatat                                                                           >  2:5488009/1‑93 (MQ=255)

               aaaaCCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATg                                                                          <  2:4404180/93‑1 (MQ=255)

                aaaCCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgc                                                                         >  1:5797711/1‑93 (MQ=255)

                  aCCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgc                                                                       >  2:3019553/1‑93 (MQ=255)

                   ccACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgcc                                                                      >  1:2363652/1‑93 (MQ=255)

                   ccACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgcc                                                                      >  2:6890595/1‑93 (MQ=255)

                    cACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccg                                                                     <  1:7427604/93‑1 (MQ=255)

                    cACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccg                                                                     <  2:5257346/93‑1 (MQ=255)

                    cACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGAATCTGTTGTCGTTGAAGCAAACTCATCAACATATgcgccg                                                                     >  2:1801405/1‑93 (MQ=255)

                      cTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccgcg                                                                   >  1:6468236/1‑93 (MQ=255)

                      cTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccgcg                                                                   >  2:6077129/1‑93 (MQ=255)

                       ttAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccgcgc                                                                  >  2:3920761/1‑93 (MQ=255)

                       ttAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccgcgc                                                                  <  1:3058823/93‑1 (MQ=255)

                        tAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccgcgcc                                                                 <  2:7232876/93‑1 (MQ=255)

                        tAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATgcgccgcgcc                                                                 >  2:4585268/1‑93 (MQ=255)

                         aaGCTTTGCAGCGTATTCATGGTTGCCTCGCCAACACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCt                                                                <  1:1060547/93‑1 (MQ=255)

                         aaGCGTTGCAGCGTATTCATGGATGCCTCGCCAAAACCAATCGCCAGAATCTATTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCt                                                                >  2:7025167/1‑93 (MQ=255)

                          aGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAGCATATGCGACGCGCCTa                                                               <  2:6672365/93‑1 (MQ=255)

                          aGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTa                                                               <  2:6095040/93‑1 (MQ=255)

                          aGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTa                                                               <  1:7110494/93‑1 (MQ=255)

                             gTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATcc                                                            >  2:8868796/1‑93 (MQ=255)

                             gTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATcc                                                            >  2:3450198/1‑93 (MQ=255)

                              ttGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATccc                                                           >  1:9317056/1‑93 (MQ=255)

                              ttGCAGCGTATTCATGGTGGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATccc                                                           <  1:6050282/93‑1 (MQ=255)

                               tGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATcccc                                                          <  2:8172973/93‑1 (MQ=255)

                                tcAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCa                                                         <  1:3093842/92‑1 (MQ=255)

                                tcAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCa                                                         <  2:7891778/92‑1 (MQ=255)

                                gCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCa                                                         <  1:1726741/93‑1 (MQ=255)

                                 cAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAg                                                        >  1:9824709/1‑93 (MQ=255)

                                   gCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTc                                                      >  2:2864609/1‑93 (MQ=255)

                                    cGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCg                                                     <  1:7976544/93‑1 (MQ=255)

                                      tATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGcc                                                   <  1:7510718/93‑1 (MQ=255)

                                      tATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGcc                                                   >  2:8389496/1‑93 (MQ=255)

                                      tATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGcc                                                   >  2:4119915/1‑93 (MQ=255)

                                       aTTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGccc                                                  <  2:761960/93‑1 (MQ=255)

                                        ttCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTTAAGCAAACTCATCAACATATGCGCCGCGCCTACCCCCAGTCGCCCa                                                 >  2:8635469/1‑93 (MQ=255)

                                        ttCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCa                                                 >  1:4628119/1‑93 (MQ=255)

                                         tCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCAc                                                >  2:233522/1‑93 (MQ=255)

                                          cATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCAcc                                               <  1:3994263/93‑1 (MQ=255)

                                           aTGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCg                                              >  2:1964103/1‑93 (MQ=255)

                                            tGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGa                                             <  1:7696453/93‑1 (MQ=255)

                                            tGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGa                                             <  1:10013190/93‑1 (MQ=255)

                                                tGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATc                                         <  2:4107664/93‑1 (MQ=255)

                                                tGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATc                                         <  1:5651831/93‑1 (MQ=255)

                                                  ccTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAg                                       >  1:246976/1‑93 (MQ=255)

                                                   cTCGCGAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACCCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCCCCGACATCAGc                                      <  2:5137178/93‑1 (MQ=255)

                                                     cGCCAAAACCAATCGCCAGTATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACAGACATCAGCAt                                    >  2:9881917/1‑93 (MQ=255)

                                                     cGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCAt                                    >  1:8240322/1‑93 (MQ=255)

                                                     cGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCAt                                    <  1:3551667/93‑1 (MQ=255)

                                                     cGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCAt                                    <  1:4685381/93‑1 (MQ=255)

                                                       ccAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATcc                                  <  1:4319376/93‑1 (MQ=255)

                                                        cAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAAATCATCAACATATGCGCCGCGCCTATCCCCAGTCGACCACCGACATCAGCACCCg                                 >  1:2570163/1‑93 (MQ=255)

                                                         aaaaCCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGc                                >  1:5507692/1‑93 (MQ=255)

                                                         aaaaCCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGc                                >  1:3011697/1‑93 (MQ=255)

                                                           aaCCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCaa                              <  1:9031637/93‑1 (MQ=255)

                                                           aaCCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCaa                              <  2:5534762/93‑1 (MQ=255)

                                                            aCCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAg                             >  1:9505364/1‑93 (MQ=255)

                                                             ccAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGc                            <  2:326579/93‑1 (MQ=255)

                                                                aTCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCa                         <  2:9681451/93‑1 (MQ=255)

                                                                 tCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAg                        <  2:4670490/93‑1 (MQ=255)

                                                                    ccAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGa                     <  2:5608182/93‑1 (MQ=255)

                                                                    ccAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGa                     <  2:9316201/93‑1 (MQ=255)

                                                                     cAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGAt                    <  2:4877791/93‑1 (MQ=255)

                                                                       gCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCa                  >  1:8802251/1‑93 (MQ=255)

                                                                       gCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCa                  >  1:2714419/1‑93 (MQ=255)

                                                                       gCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCa                  <  1:1177493/93‑1 (MQ=255)

                                                                         aTCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCAcc                <  1:5027676/93‑1 (MQ=255)

                                                                          tCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCAccc               >  1:3938336/1‑93 (MQ=255)

                                                                            tgttgtGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCgg             >  1:6018674/1‑93 (MQ=255)

                                                                             gttgtGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCggc            >  1:3854872/1‑92 (MQ=255)

                                                                             gttgtGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGa            >  2:1581838/1‑93 (MQ=255)

                                                                               tgtgGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATGCCCAGTAGACCACCGGCATCAGCATCCGCAAGCCCAGGGATCACCCGGACa          >  2:3982187/1‑93 (MQ=255)

                                                                                gtgGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGCTCACCCGGACAt         >  1:3410959/1‑93 (MQ=255)

                                                                                gtgGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACAt         >  1:1278035/1‑93 (MQ=255)

                                                                                 tgGTTGAAGCAAACTCATCAACATATGCCCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCCCCCGGACATg        <  1:6185021/93‑1 (MQ=255)

                                                                                  ggTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGt       >  2:6488428/1‑93 (MQ=255)

                                                                                      gAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCa   >  2:2723121/1‑93 (MQ=255)

TTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAG  >  NC_000913/1598837‑1599016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATTTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCG  >  NC_000913/1598834‑1599018

AATTTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGC                                                                                             >  SRR4302540.216629/1‑94 (MQ=60)

 ATTTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCA                                                                                            >  SRR4302540.7940832/1‑94 (MQ=60)

 ATTTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCA                                                                                            >  SRR4302540.7279393/1‑94 (MQ=60)

   TTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAA                                                                                          <  SRR4302540.10085117/94‑1 (MQ=60)

    TGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAAC                                                                                         >  SRR4302540.1931007/1‑94 (MQ=60)

      CTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATAGCCAGCATCTGTTGTGGTTGAAGCAAACTC                                                                                       >  SRR4302540.6059118/1‑94 (MQ=60)

       TGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCA                                                                                      >  SRR4302540.6051983/1‑94 (MQ=60)

        GTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCAT                                                                                     <  SRR4302540.712281/94‑1 (MQ=60)

         TGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATC                                                                                    >  SRR4302540.6252362/1‑94 (MQ=60)

          GACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCA                                                                                   <  SRR4302540.6368685/94‑1 (MQ=60)

          GACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCA                                                                                   <  SRR4302540.8925749/94‑1 (MQ=60)

            CGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAAC                                                                                 >  SRR4302540.4117281/1‑94 (MQ=60)

            CGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAAC                                                                                 <  SRR4302540.2488365/94‑1 (MQ=60)

             GAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACA                                                                                >  SRR4302540.3946028/1‑94 (MQ=60)

             GAAATAAAACCACTTAAGCGTTGCAGCGTAATCATGGTTGCCTCGCCAAATCCAATCGCCAGCAGCTATTGTTGTTGAAGCAAACTCATCAACA                                                                                <  SRR4302540.6084765/94‑1 (MQ=60)

              AAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACAT                                                                               <  SRR4302540.190193/94‑1 (MQ=60)

               AATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACGTA                                                                              >  SRR4302540.8220648/1‑94 (MQ=60)

               AATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATA                                                                              >  SRR4302540.6280069/1‑94 (MQ=60)

                ATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATAT                                                                             >  SRR4302540.8648742/1‑94 (MQ=60)

                ATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATAT                                                                             <  SRR4302540.3250445/94‑1 (MQ=60)

                 TAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATG                                                                            <  SRR4302540.3418402/94‑1 (MQ=60)

                  AAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGC                                                                           >  SRR4302540.5861776/1‑94 (MQ=60)

                   AAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGAAAACTAATCAACATATGCG                                                                          >  SRR4302540.8755528/1‑94 (MQ=60)

                     ACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCC                                                                        >  SRR4302540.2389109/1‑94 (MQ=60)

                       CACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGC                                                                      <  SRR4302540.7510347/94‑1 (MQ=60)

                       CACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGC                                                                      >  SRR4302540.6237467/1‑94 (MQ=60)

                        ACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCG                                                                     <  SRR4302540.8250182/94‑1 (MQ=60)

                        ACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCG                                                                     >  SRR4302540.6540209/1‑94 (MQ=60)

                        ACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGAATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCGGCG                                                                     >  SRR4302540.3016926/1‑94 (MQ=60)

                         CTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGC                                                                    >  SRR4302540.4651212/1‑94 (MQ=60)

                          TTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCC                                                                   <  SRR4302540.3092386/94‑1 (MQ=60)

                           TAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCT                                                                  <  SRR4302540.7665482/94‑1 (MQ=60)

                            AAGCTTTGCAGCGTATTCATGGTTGCCTCGCCAACACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTA                                                                 <  SRR4302540.1071342/94‑1 (MQ=60)

                            AAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTA                                                                 <  SRR4302540.9602010/94‑1 (MQ=60)

                            AAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTA                                                                 <  SRR4302540.1083711/94‑1 (MQ=60)

                             AGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTAT                                                                >  SRR4302540.8082238/1‑94 (MQ=60)

                             AGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTAT                                                                <  SRR4302540.7189631/94‑1 (MQ=60)

                                GTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCC                                                             >  SRR4302540.9428238/1‑94 (MQ=60)

                                GTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCC                                                             <  SRR4302540.3957518/94‑1 (MQ=60)

                                 TTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCC                                                            <  SRR4302540.6117521/94‑1 (MQ=60)

                                 TTGCAGCGTATTCATGGTGGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCC                                                            <  SRR4302540.6117522/94‑1 (MQ=60)

                                  TGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCA                                                           <  SRR4302540.4689303/94‑1 (MQ=60)

                                   TCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAG                                                          <  SRR4302540.3127764/94‑1 (MQ=60)

                                   GCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAG                                                          <  SRR4302540.1745378/94‑1 (MQ=60)

                                   GCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAG                                                          >  SRR4302540.9939649/1‑94 (MQ=60)

                                     AGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTC                                                        >  SRR4302540.8225503/1‑94 (MQ=60)

                                     AGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTC                                                        >  SRR4302540.9320021/1‑94 (MQ=60)

                                       CGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGC                                                      <  SRR4302540.8069468/94‑1 (MQ=60)

                                        GTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCC                                                     >  SRR4302540.1018872/1‑94 (MQ=60)

                                        GTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCC                                                     <  SRR4302540.6664127/94‑1 (MQ=60)

                                        GTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCC                                                     >  SRR4302540.4239687/1‑94 (MQ=60)

                                        GTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCC                                                     >  SRR4302540.4652665/1‑94 (MQ=60)

                                         TATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCC                                                    <  SRR4302540.7594672/94‑1 (MQ=60)

                                          ATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCGCAGTCGCCCC                                                   >  SRR4302540.2545417/1‑94 (MQ=60)

                                          ATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCA                                                   <  SRR4302540.7129311/94‑1 (MQ=60)

                                          ATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCA                                                   >  SRR4302540.4677699/1‑94 (MQ=60)

                                          ATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCA                                                   >  SRR4302540.2545418/1‑94 (MQ=60)

                                          ATTCATGGTTGCCTCGCCAAAACCAATCGCCAGAATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCA                                                   >  SRR4302540.4926025/1‑94 (MQ=60)

                                           TTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCAC                                                  <  SRR4302540.837381/94‑1 (MQ=60)

                                           TTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCAC                                                  >  SRR4302540.1342423/1‑94 (MQ=60)

                                            TCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACC                                                 <  SRR4302540.1658256/94‑1 (MQ=60)

                                             CATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCG                                                <  SRR4302540.4037249/94‑1 (MQ=60)

                                               TGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGAC                                              <  SRR4302540.7784187/94‑1 (MQ=60)

                                               TGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGAC                                              <  SRR4302540.10129770/94‑1 (MQ=60)

                                                   TGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCA                                          <  SRR4302540.5714059/94‑1 (MQ=60)

                                                   TGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCA                                          <  SRR4302540.5714058/94‑1 (MQ=60)

                                                   TGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCA                                          >  SRR4302540.9312681/1‑94 (MQ=60)

                                                    GCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAG                                         >  SRR4302540.249366/1‑94 (MQ=60)

                                                     CCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGC                                        <  SRR4302540.1129721/94‑1 (MQ=60)

                                                      CTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCA                                       <  SRR4302540.5817167/94‑1 (MQ=60)

                                                      CTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCA                                       <  SRR4302540.3059352/94‑1 (MQ=60)

                                                      CTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCA                                       >  SRR4302540.4758226/1‑94 (MQ=60)

                                                      CTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCA                                       >  SRR4302540.4105587/1‑94 (MQ=60)

                                                       TCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCAT                                      >  SRR4302540.8336093/1‑94 (MQ=60)

                                                       TCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCAT                                      >  SRR4302540.2731742/1‑94 (MQ=60)

                                                        CGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATC                                     <  SRR4302540.3590471/94‑1 (MQ=60)

                                                        CGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATC                                     <  SRR4302540.4735496/94‑1 (MQ=60)

                                                          CCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCG                                   <  SRR4302540.4366396/94‑1 (MQ=60)

                                                          CCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAAATCATCAACATATGCGCCGCGCCTATCCCCAGTCGACCACCGACATCAGCACCCG                                   >  SRR4302540.2597876/1‑94 (MQ=60)

                                                           CAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGC                                  >  SRR4302540.3044822/1‑94 (MQ=60)

                                                           CAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGC                                  >  SRR4302540.5568352/1‑94 (MQ=60)

                                                            AAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCA                                 <  SRR4302540.3640819/94‑1 (MQ=60)

                                                            AAAACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCA                                 >  SRR4302540.5378720/1‑94 (MQ=60)

                                                              AACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAG                               <  SRR4302540.9140023/94‑1 (MQ=60)

                                                              AACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAG                               >  SRR4302540.9617982/1‑94 (MQ=60)

                                                              AACCAATCGCCAGAATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAG                               >  SRR4302540.516495/1‑94 (MQ=60)

                                                               ACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGC                              >  SRR4302540.3819953/1‑94 (MQ=60)

                                                               ACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGC                              <  SRR4302540.3112695/94‑1 (MQ=60)

                                                               ACCAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGC                              >  SRR4302540.7290508/1‑94 (MQ=60)

                                                                 CAATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCC                            <  SRR4302540.7597043/94‑1 (MQ=60)

                                                                  AATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCA                           <  SRR4302540.6254585/94‑1 (MQ=60)

                                                                  AATCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCA                           <  SRR4302540.7517112/94‑1 (MQ=60)

                                                                    TCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGG                         <  SRR4302540.6906810/94‑1 (MQ=60)

                                                                    TCGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGG                         <  SRR4302540.2314473/94‑1 (MQ=60)

                                                                     CGCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGG                        <  SRR4302540.9445745/94‑1 (MQ=60)

                                                                      GCCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGA                       <  SRR4302540.882383/94‑1 (MQ=60)

                                                                       CCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGAT                      <  SRR4302540.9712736/94‑1 (MQ=60)

                                                                       CCAGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGAC                      >  SRR4302540.737713/1‑94 (MQ=60)

                                                                         AGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCA                    >  SRR4302540.2743689/1‑94 (MQ=60)

                                                                         AGCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCA                    >  SRR4302540.8904011/1‑94 (MQ=60)

                                                                          GCATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCAC                   <  SRR4302540.1189662/94‑1 (MQ=60)

                                                                            ATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCC                 >  SRR4302540.3980889/1‑94 (MQ=60)

                                                                            ATCTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCC                 <  SRR4302540.5082089/94‑1 (MQ=60)

                                                                             TCTGTTGTGGTTGTAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCG                >  SRR4302540.7886189/1‑94 (MQ=60)

                                                                              CTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGG               >  SRR4302540.6085426/1‑94 (MQ=60)

                                                                              CTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGG               >  SRR4302540.6085427/1‑94 (MQ=60)

                                                                              CTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGG               <  SRR4302540.2739481/94‑1 (MQ=60)

                                                                              CTGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGG               <  SRR4302540.407126/94‑1 (MQ=60)

                                                                               TGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGC              >  SRR4302540.3896785/1‑94 (MQ=60)

                                                                               TGTTGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGA              >  SRR4302540.3053917/1‑94 (MQ=60)

                                                                                  TGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGCTCACCCGGACAT           >  SRR4302540.3448110/1‑94 (MQ=60)

                                                                                  TGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGCCAT           >  SRR4302540.1291210/1‑94 (MQ=60)

                                                                                  TGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACAT           >  SRR4302540.2123244/1‑94 (MQ=60)

                                                                                  TGTGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACAT           >  SRR4302540.1291211/1‑94 (MQ=60)

                                                                                    TGGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGT         <  SRR4302540.6253903/94‑1 (MQ=60)

                                                                                    TGGTTGAAGCAAACTCATCAACATATGCCCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCCCCCGGACATGT         <  SRR4302540.6253902/94‑1 (MQ=60)

                                                                                     GGTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTT        <  SRR4302540.7896788/94‑1 (MQ=60)

                                                                                      GTTGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTG       >  SRR4302540.3990237/1‑94 (MQ=60)

                                                                                        TGAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCA     <  SRR4302540.5700976/94‑1 (MQ=60)

                                                                                         GAAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGCCATGTTGCAG    >  SRR4302540.3543125/1‑94 (MQ=60)

                                                                                          AAGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGC   >  SRR4302540.1156078/1‑94 (MQ=60)

                                                                                           AGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCG  <  SRR4302540.9283154/94‑1 (MQ=60)

                                                                                           AGCAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCG  <  SRR4302540.6129971/94‑1 (MQ=60)

AATTTGCTGTGACGAAATAAAACCACTTAAGCGTTGCAGCGTATTCATGGTTGCCTCGCCAAAACCAATCGCCAGCATCTGTTGTGGTTGAAGTAAACTCATCAACATATGCGCCGCGCCTATCCCCAGTCGCCCACCGACATCAGCATCCGCAAGCCCAGGGATCACCCGGACATGTTGCAGCG  >  NC_000913/1598834‑1599018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`