Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,617,966	T→C	N125N (AAT→AAC)	marA →	MarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,617,966	0	T	C	99.4%	996.9 / ‑3.8	306	N125N (AAT→AAC)	marA	MarA
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (143/161); total (143/163)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 311 total aligned reads displayed.

CCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCT  >  NC_000913/1617876‑1618055

ccGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCa                                                                                         >  1:5030490/1‑93 (MQ=255)

ccGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAAACa                                                                                         >  2:1857772/1‑93 (MQ=255)

 cGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCAt                                                                                        <  2:2656842/93‑1 (MQ=255)

  gAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCAtt                                                                                       >  2:1641422/1‑93 (MQ=255)

   aaCCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTa                                                                                      <  2:3244759/93‑1 (MQ=255)

   aaCCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTa                                                                                      >  1:2525827/1‑93 (MQ=255)

    aCCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTac                                                                                     <  2:4027359/93‑1 (MQ=255)

     ccTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTaca                                                                                    >  2:7102485/1‑93 (MQ=255)

      cTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaa                                                                                   <  1:7013091/93‑1 (MQ=255)

      cTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaa                                                                                   >  1:9385071/1‑93 (MQ=255)

      cTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaa                                                                                   <  1:4320776/93‑1 (MQ=255)

       ttCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaac                                                                                  <  1:3506246/93‑1 (MQ=255)

       ttCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaac                                                                                  >  1:8932949/1‑93 (MQ=255)

        tCAAAAATTCCTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaaca                                                                                 <  2:3463998/93‑1 (MQ=255)

        tCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTacaaca                                                                                 >  1:6313066/1‑93 (MQ=255)

         cAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAg                                                                                <  2:5535451/93‑1 (MQ=255)

          aaaaaTTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGc                                                                               <  1:5298842/93‑1 (MQ=255)

          aaaaaTTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGc                                                                               <  2:758801/93‑1 (MQ=255)

               ttACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGtt                                                                          <  2:9524498/93‑1 (MQ=255)

               ttACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGtt                                                                          <  2:8911260/93‑1 (MQ=255)

                   tttGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGaaa                                                                      >  1:9812555/1‑93 (MQ=255)

                    ttGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGaaaa                                                                     <  2:8041344/93‑1 (MQ=255)

                     tGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAAc                                                                    >  2:7206778/1‑93 (MQ=255)

                      gATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACg                                                                   <  2:5504338/93‑1 (MQ=255)

                      gATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACg                                                                   >  1:4079567/1‑93 (MQ=255)

                       aTGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGt                                                                  <  2:6504380/93‑1 (MQ=255)

                       aTGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGt                                                                  >  1:8641311/1‑93 (MQ=255)

                         gTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGa                                                                <  1:10008338/93‑1 (MQ=255)

                          ttCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAc                                                               >  1:2746883/1‑93 (MQ=255)

                          ttCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAc                                                               >  1:6208511/1‑93 (MQ=255)

                          ttCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAc                                                               >  2:9080111/1‑93 (MQ=255)

                          tgcggccgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAc                                                               <  2:4673607/89‑1 (MQ=255)

                            ccgccgCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACaa                                                             <  1:2105458/93‑1 (MQ=255)

                            ccgccgCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACaa                                                             <  1:8122879/93‑1 (MQ=255)

                             cgccgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAAc                                                            <  1:7640208/93‑1 (MQ=255)

                              gccgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACA‑CCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGt                                                          >  2:7245345/1‑93 (MQ=255)

                               ccgcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGt                                                          <  1:8505685/93‑1 (MQ=255)

                                 gcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCa                                                        <  1:8693690/93‑1 (MQ=255)

                                 gcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCa                                                        <  1:7529764/93‑1 (MQ=255)

                                 gcATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCa                                                        >  2:2456335/1‑93 (MQ=255)

                                     aaaTACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGa                                                    <  1:4886247/93‑1 (MQ=255)

                                       aTACCGGATGACCAATATGCAGGGCGAATCGAGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAgg                                                  >  1:184517/1‑93 (MQ=255)

                                         aCCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCa                                                >  2:4615339/1‑93 (MQ=255)

                                          ccGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCaa                                               <  2:2393090/93‑1 (MQ=255)

                                          ccGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCaa                                               <  2:9659049/93‑1 (MQ=255)

                                           cGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAAt                                              <  1:3618988/93‑1 (MQ=255)

                                            ggATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCAc                                                       <  2:390168/83‑1 (MQ=255)

                                            ggATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATc                                             <  2:4408739/93‑1 (MQ=255)

                                             gATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCa                                            <  1:3556956/93‑1 (MQ=255)

                                              aTGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCAt                                           <  2:7891108/93‑1 (MQ=255)

                                                gACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGa                                         <  2:2849885/93‑1 (MQ=255)

                                                 aCCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGaa                                        <  1:6505385/93‑1 (MQ=255)

                                                  ccAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGaaa                                       <  1:8328892/93‑1 (MQ=255)

                                                   cAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAc                                      >  2:1333492/1‑93 (MQ=255)

                                                    aaTATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAcc                                     <  2:2292526/93‑1 (MQ=255)

                                                    aaTATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAcc                                     <  1:4380018/93‑1 (MQ=255)

                                                    aaTATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAcc                                     >  1:1611014/1‑93 (MQ=255)

                                                    aaTATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAcc                                     <  2:3064277/93‑1 (MQ=255)

                                                    aaTATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAcc                                     <  1:684672/93‑1 (MQ=255)

                                                     atatGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTATAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCa                                    <  2:5751905/93‑1 (MQ=255)

                                                     atatGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCa                                    <  1:6029696/93‑1 (MQ=255)

                                                      tatGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAGCCAc                                   >  1:5882533/1‑93 (MQ=255)

                                                      tatGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCAc                                   >  2:4242198/1‑93 (MQ=255)

                                                       atGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACt                                  <  1:6634635/93‑1 (MQ=255)

                                                        tGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACtt                                 <  2:1461410/93‑1 (MQ=255)

                                                          cAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAAAGTGACAACGTCACTGAGGCAATCATGAAACCACTTTc                               >  2:4964835/1‑93 (MQ=255)

                                                           aGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCa                              <  2:9170062/93‑1 (MQ=255)

                                                           aGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCa                              >  2:6055710/1‑93 (MQ=255)

                                                           aGGGCGAATCGCAATTTTTACATCCATTAAGCCAGTACAGCAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCa                              <  2:220027/93‑1 (MQ=255)

                                                            gggCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCAt                             >  2:6727034/1‑93 (MQ=255)

                                                             ggCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATc                            <  1:3931480/93‑1 (MQ=255)

                                                             ggCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATc                            >  1:2009826/1‑93 (MQ=255)

                                                                 aaTCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCa                        <  1:5113349/93‑1 (MQ=255)

                                                                    cgcgCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATa                     <  2:5679522/93‑1 (MQ=255)

                                                                     gctcTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATag                    <  1:3377823/90‑1 (MQ=255)

                                                                     gcgcTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATag                    >  2:449085/1‑93 (MQ=255)

                                                                      cgcTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagc                   <  1:2991034/93‑1 (MQ=255)

                                                                       gcTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagca                  >  2:8678256/1‑93 (MQ=255)

                                                                        cTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcag                 >  2:6175106/1‑93 (MQ=255)

                                                                        cTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcag                 <  1:5403564/93‑1 (MQ=255)

                                                                         tttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcagc                >  2:6887724/1‑93 (MQ=255)

                                                                         tttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcagc                >  2:1050744/1‑93 (MQ=255)

                                                                         tttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATagcagc                >  1:3831600/1‑93 (MQ=255)

                                                                         tttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCAGCAATagcagc                >  1:3441378/1‑93 (MQ=255)

                                                                          ttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt               >  2:8229940/1‑93 (MQ=255)

                                                                          ttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt               >  2:5577896/1‑93 (MQ=255)

                                                                          ttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt               >  2:4998433/1‑93 (MQ=255)

                                                                          ttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt               >  1:4601346/1‑93 (MQ=255)

                                                                          ttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt               >  1:6703898/1‑93 (MQ=255)

                                                                          ttttACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCt               <  2:4151258/93‑1 (MQ=255)

                                                                             tACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcg            >  2:7714414/1‑93 (MQ=255)

                                                                             tACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcg            <  2:3750466/93‑1 (MQ=255)

                                                                              aCATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTgcgc           >  1:1497585/1‑93 (MQ=255)

                                                                               cATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCt          <  1:167162/93‑1 (MQ=255)

                                                                                aTCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCtt         >  2:5274601/1‑93 (MQ=255)

                                                                                  ccATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAt       <  1:5480309/93‑1 (MQ=255)

                                                                                  ccATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAt       <  1:9126694/93‑1 (MQ=255)

                                                                                   cATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAtt      <  2:2987748/93‑1 (MQ=255)

                                                                                    aTTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtc     >  1:7296769/1‑93 (MQ=255)

                                                                                      tAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATtctc   <  1:5096346/93‑1 (MQ=255)

CCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCT  >  NC_000913/1617876‑1618055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTC  >  NC_000913/1617874‑1618059

ACCCGAACCTTCAAAAATTACTTTGATTTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACC                                                                                              >  SRR4302540.2596442/1‑94 (MQ=60)

ACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACC                                                                                              >  SRR4302540.8466871/1‑94 (MQ=60)

 CCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCA                                                                                             >  SRR4302540.5084927/1‑94 (MQ=60)

 CCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCA                                                                                             >  SRR4302540.1451869/1‑94 (MQ=60)

 CCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCA                                                                                             <  SRR4302540.660053/94‑1 (MQ=60)

   CGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATT                                                                                           >  SRR4302540.5534496/1‑94 (MQ=60)

    GAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTA                                                                                          >  SRR4302540.2974418/1‑94 (MQ=60)

    GAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTA                                                                                          >  SRR4302540.2553091/1‑94 (MQ=60)

    GAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTA                                                                                          <  SRR4302540.6600833/94‑1 (MQ=60)

      ACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACA                                                                                        <  SRR4302540.349081/94‑1 (MQ=60)

       CCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAA                                                                                       >  SRR4302540.9496779/1‑94 (MQ=60)

        CTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAAC                                                                                      <  SRR4302540.7090953/94‑1 (MQ=60)

        CTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAAC                                                                                      >  SRR4302540.9037314/1‑94 (MQ=60)

        CTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAAC                                                                                      <  SRR4302540.4367816/94‑1 (MQ=60)

         TTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACA                                                                                     >  SRR4302540.6383367/1‑94 (MQ=60)

         TTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACA                                                                                     >  SRR4302540.6383366/1‑94 (MQ=60)

         TTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACA                                                                                     >  SRR4302540.9295081/1‑94 (MQ=60)

         TTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACA                                                                                     <  SRR4302540.3544441/94‑1 (MQ=60)

          TCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAG                                                                                    <  SRR4302540.8084966/94‑1 (MQ=60)

            AAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCT                                                                                  <  SRR4302540.5356811/94‑1 (MQ=60)

              AAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAG                                                                                <  SRR4302540.1201997/94‑1 (MQ=60)

               AATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGT                                                                               <  SRR4302540.4650323/94‑1 (MQ=60)

                ATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTT                                                                              >  SRR4302540.2093127/1‑94 (MQ=60)

                ATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTT                                                                              >  SRR4302540.9366552/1‑94 (MQ=60)

                   ACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAA                                                                           >  SRR4302540.5362661/1‑94 (MQ=60)

                   ACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAA                                                                           >  SRR4302540.546248/1‑94 (MQ=60)

                    CTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAA                                                                          <  SRR4302540.369116/94‑1 (MQ=60)

                    CTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAA                                                                          >  SRR4302540.9927393/1‑94 (MQ=60)

                    CTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAA                                                                          <  SRR4302540.276390/94‑1 (MQ=60)

                     TTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAA                                                                         <  SRR4302540.9232625/94‑1 (MQ=60)

                      TTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAAC                                                                        >  SRR4302540.4144003/1‑94 (MQ=60)

                      TTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAAC                                                                        >  SRR4302540.6570623/1‑94 (MQ=60)

                      TTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAAC                                                                        >  SRR4302540.6570622/1‑94 (MQ=60)

                      TTGAAGTGCCGCCGCATAAATACCGTATGGCCAATATGCAGGGCGAATCGCCCTTTTTACATCCATTAAACCATGACAACAGCTAGTTGAAAAC                                                                        <  SRR4302540.2378721/94‑1 (MQ=60)

                       TGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACG                                                                       >  SRR4302540.4123554/1‑94 (MQ=60)

                       TGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACG                                                                       <  SRR4302540.4275788/94‑1 (MQ=60)

                       TGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACG                                                                       >  SRR4302540.5963681/1‑94 (MQ=60)

                        GATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGT                                                                      >  SRR4302540.8741687/1‑94 (MQ=60)

                         ATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTG                                                                     <  SRR4302540.7448044/94‑1 (MQ=60)

                         ATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTG                                                                     >  SRR4302540.3535088/1‑94 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   >  SRR4302540.6277613/1‑94 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   >  SRR4302540.5757731/1‑94 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   <  SRR4302540.6008648/94‑1 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   <  SRR4302540.10124890/94‑1 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   <  SRR4302540.1589211/94‑1 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   <  SRR4302540.9918396/94‑1 (MQ=60)

                           GTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGAC                                                                   >  SRR4302540.2776552/1‑94 (MQ=60)

                            TTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACA                                                                  <  SRR4302540.9683307/94‑1 (MQ=60)

                             TCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAA                                                                 <  SRR4302540.1970328/94‑1 (MQ=60)

                              CCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAAC                                                                <  SRR4302540.2128056/94‑1 (MQ=60)

                              CCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAAC                                                                <  SRR4302540.8217157/94‑1 (MQ=60)

                              CCGCCGCATAAATACCGGATGACCAAGATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATGACAACAGCTAGTTGAAAACGTGACAAC                                                                <  SRR4302540.2078643/94‑1 (MQ=60)

                               CGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAAagagacaaag                                                               >  SRR4302540.7802028/1‑84 (MQ=60)

                               CGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACG                                                               >  SRR4302540.7366708/1‑94 (MQ=60)

                               CGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACG                                                               <  SRR4302540.7726186/94‑1 (MQ=60)

                               CGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACG                                                               <  SRR4302540.2177441/94‑1 (MQ=60)

                               CGCCGCATAAAGACCGGATGACCAATTTGCGGGGTGAATCGCGCTTTTTACATGCATGAAACCATTACAACAGGTAGTTGAAAACGTGACAACG                                                               <  SRR4302540.2177440/94‑1 (MQ=60)

                                GCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGT                                                              >  SRR4302540.3992664/1‑94 (MQ=60)

                                 CCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTC                                                             <  SRR4302540.8604533/94‑1 (MQ=60)

                                 CCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTC                                                             <  SRR4302540.3641789/94‑1 (MQ=60)

                                  CGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCA                                                            >  SRR4302540.166543/1‑94 (MQ=60)

                                  CGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCA                                                            <  SRR4302540.911446/94‑1 (MQ=60)

                                   GCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACTTGACAACGTCAC                                                           >  SRR4302540.5285061/1‑94 (MQ=60)

                                   GCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC                                                           <  SRR4302540.8794616/94‑1 (MQ=60)

                                   GCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC                                                           <  SRR4302540.7614052/94‑1 (MQ=60)

                                   GCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC                                                           >  SRR4302540.5285062/1‑94 (MQ=60)

                                   GCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCAC                                                           <  SRR4302540.7614053/94‑1 (MQ=60)

                                    CATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACT                                                          >  SRR4302540.3577924/1‑94 (MQ=60)

                                     ATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTG                                                         >  SRR4302540.4770832/1‑94 (MQ=60)

                                     ATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTG                                                         >  SRR4302540.8634374/1‑94 (MQ=60)

                                      TAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGA                                                        <  SRR4302540.4502768/94‑1 (MQ=60)

                                       AAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAG                                                       <  SRR4302540.4651179/94‑1 (MQ=60)

                                       AAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAG                                                       <  SRR4302540.4938742/94‑1 (MQ=60)

                                       AAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAG                                                       >  SRR4302540.5087113/1‑94 (MQ=60)

                                        AATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGG                                                      <  SRR4302540.9121540/94‑1 (MQ=60)

                                        AATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGG                                                      >  SRR4302540.71259/1‑94 (MQ=60)

                                        AATACCGGATGACCAATATGCAGGGCGAATCGAGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGG                                                      >  SRR4302540.186355/1‑94 (MQ=60)

                                         ATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGC                                                     >  SRR4302540.3812996/1‑94 (MQ=60)

                                          TACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCA                                                    >  SRR4302540.3459861/1‑94 (MQ=60)

                                          AACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCA                                                    <  SRR4302540.6996158/94‑1 (MQ=60)

                                            CCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAAT                                                  >  SRR4302540.400337/1‑94 (MQ=60)

                                            CCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAAT                                                  <  SRR4302540.8325279/94‑1 (MQ=60)

                                             CGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTaagcgctag                                                 >  SRR4302540.394197/1‑85 (MQ=60)

                                             CGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATC                                                 <  SRR4302540.3658576/94‑1 (MQ=60)

                                             CGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATC                                                 <  SRR4302540.6709186/94‑1 (MQ=60)

                                              GGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCA                                                <  SRR4302540.8768269/94‑1 (MQ=60)

                                              GGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCA                                                <  SRR4302540.8768268/94‑1 (MQ=60)

                                              CGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCA                                                <  SRR4302540.7456078/94‑1 (MQ=60)

                                               GATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCAT                                               <  SRR4302540.3595821/94‑1 (MQ=60)

                                                 TGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGA                                             >  SRR4302540.2683811/1‑94 (MQ=60)

                                                   ACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAA                                           >  SRR4302540.8550125/1‑94 (MQ=60)

                                                   ACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAA                                           <  SRR4302540.6577689/94‑1 (MQ=60)

                                                    CCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAC                                          <  SRR4302540.8425560/94‑1 (MQ=60)

                                                    CCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAC                                          <  SRR4302540.7697491/94‑1 (MQ=60)

                                                    CCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAC                                          <  SRR4302540.8425559/94‑1 (MQ=60)

                                                    CCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAAC                                          >  SRR4302540.6357824/1‑94 (MQ=60)

                                                     CAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACC                                         >  SRR4302540.3473367/1‑94 (MQ=60)

                                                     CAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACC                                         >  SRR4302540.1628661/1‑94 (MQ=60)

                                                      AATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCA                                        <  SRR4302540.692019/94‑1 (MQ=60)

                                                      AATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCA                                        <  SRR4302540.4427573/94‑1 (MQ=60)

                                                      AATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAAGCATGAAACCA                                        <  SRR4302540.692020/94‑1 (MQ=60)

                                                       ATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAGCCAC                                       >  SRR4302540.5947542/1‑94 (MQ=60)

                                                       ATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCAC                                       <  SRR4302540.6096608/94‑1 (MQ=60)

                                                       ATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCAC                                       >  SRR4302540.6821344/1‑94 (MQ=60)

                                                         ATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTT                                     >  SRR4302540.195401/1‑94 (MQ=60)

                                                         ATGCAGGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTT                                     <  SRR4302540.6708062/94‑1 (MQ=60)

                                                         ATGCAGGGCGAATCGCGCGTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTT                                     <  SRR4302540.6708061/94‑1 (MQ=60)

                                                              GGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATC                                <  SRR4302540.4422742/94‑1 (MQ=60)

                                                              GGGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATC                                >  SRR4302540.2031561/1‑94 (MQ=60)

                                                               GGCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCC                               <  SRR4302540.3973983/94‑1 (MQ=60)

                                                                GCGAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCG                              <  SRR4302540.8352240/94‑1 (MQ=60)

                                                                  GAATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCA                            >  SRR4302540.609945/1‑94 (MQ=60)

                                                                   AATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAA                           <  SRR4302540.5168697/94‑1 (MQ=60)

                                                                   AATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAA                           <  SRR4302540.5168696/94‑1 (MQ=60)

                                                                   AATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAA                           <  SRR4302540.5168695/94‑1 (MQ=60)

                                                                    ATCGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAAT                          <  SRR4302540.5040633/94‑1 (MQ=60)

                                                                      CGCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAG                        <  SRR4302540.7375998/94‑1 (MQ=60)

                                                                       GCTCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGC                       <  SRR4302540.3414717/94‑1 (MQ=60)

                                                                       GCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGC                       <  SRR4302540.1763254/94‑1 (MQ=60)

                                                                       GCGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGC                       <  SRR4302540.8805155/94‑1 (MQ=60)

                                                                        CGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCA                      <  SRR4302540.3024009/94‑1 (MQ=60)

                                                                        CGCTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCA                      <  SRR4302540.6831463/94‑1 (MQ=60)

                                                                          CTTTTTACATCCATTAAACCATTTCAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCCCTTTCATCCGCAATAGCAGC                    <  SRR4302540.8429773/94‑1 (MQ=60)

                                                                          CTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGC                    <  SRR4302540.5462970/94‑1 (MQ=60)

                                                                          CTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGC                    >  SRR4302540.4375015/1‑94 (MQ=60)

                                                                          CTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGC                    >  SRR4302540.4375016/1‑94 (MQ=60)

                                                                          CTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGC                    >  SRR4302540.3873295/1‑94 (MQ=60)

                                                                          CTTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCAGCAATAGCAGC                    >  SRR4302540.3478794/1‑94 (MQ=60)

                                                                           TTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGATACCACTTTCATCCGCAATAGCAGCT                   >  SRR4302540.6777888/1‑94 (MQ=60)

                                                                           TTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCT                   >  SRR4302540.4650794/1‑94 (MQ=60)

                                                                           TTTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCT                   >  SRR4302540.6777889/1‑94 (MQ=60)

                                                                            TTTTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTG                  <  SRR4302540.5423020/94‑1 (MQ=60)

                                                                             TTTACATCCATTAACCCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGC                 <  SRR4302540.9034812/94‑1 (MQ=60)

                                                                              TTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCG                >  SRR4302540.3282057/1‑94 (MQ=60)

                                                                              TTACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCG                >  SRR4302540.560334/1‑94 (MQ=60)

                                                                               TACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGC               >  SRR4302540.1514070/1‑94 (MQ=60)

                                                                                ACATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCT              <  SRR4302540.6145497/94‑1 (MQ=60)

                                                                                 CATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTT             <  SRR4302540.168839/94‑1 (MQ=60)

                                                                                 CATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTT             >  SRR4302540.3304923/1‑94 (MQ=60)

                                                                                  ATCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTA            >  SRR4302540.2856109/1‑94 (MQ=60)

                                                                                   TCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAT           >  SRR4302540.3598599/1‑94 (MQ=60)

                                                                                   TCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAT           >  SRR4302540.4940044/1‑94 (MQ=60)

                                                                                   TCCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTAT           <  SRR4302540.1659364/94‑1 (MQ=60)

                                                                                    CCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATT          <  SRR4302540.9236401/94‑1 (MQ=60)

                                                                                    CCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATT          <  SRR4302540.3942345/94‑1 (MQ=60)

                                                                                    CCATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATT          <  SRR4302540.5540634/94‑1 (MQ=60)

                                                                                     CATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTC         >  SRR4302540.7378202/1‑94 (MQ=60)

                                                                                      ATTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCT        <  SRR4302540.3570835/94‑1 (MQ=60)

                                                                                       TTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC       >  SRR4302540.8662828/1‑94 (MQ=60)

                                                                                       TTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC       >  SRR4302540.3287081/1‑94 (MQ=60)

                                                                                       TTAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTC       <  SRR4302540.1732637/94‑1 (MQ=60)

                                                                                        TAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCT      <  SRR4302540.5802693/94‑1 (MQ=60)

                                                                                        TAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCT      <  SRR4302540.5151492/94‑1 (MQ=60)

                                                                                        TAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCT      >  SRR4302540.153327/1‑94 (MQ=60)

                                                                                        TAAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCT      <  SRR4302540.5151491/94‑1 (MQ=60)

                                                                                         AAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTT     >  SRR4302540.1519780/1‑94 (MQ=60)

                                                                                         AAACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTT     >  SRR4302540.4211367/1‑94 (MQ=60)

                                                                                          AACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTT    <  SRR4302540.3693478/94‑1 (MQ=60)

                                                                                          AACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTT    >  SRR4302540.1698070/1‑94 (MQ=60)

                                                                                          AACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTT    <  SRR4302540.7223585/94‑1 (MQ=60)

                                                                                          AACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTT    <  SRR4302540.1611491/94‑1 (MQ=60)

                                                                                          AACCATTACAACAGCTAGTTGAAAACCTGACAACGTCACTGAGGCAATCATGAAACCCCTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTT    <  SRR4302540.2604117/94‑1 (MQ=60)

                                                                                           ACCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTT   >  SRR4302540.8446670/1‑94 (MQ=60)

                                                                                            CCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTC  <  SRR4302540.4240729/94‑1 (MQ=60)

                                                                                            CCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTC  >  SRR4302540.703948/1‑94 (MQ=60)

ACCCGAACCTTCAAAAATTACTTTGATGTTCCGCCGCATAAATACCGGATGACCAATATGCAGGGCGAATCGCGCTTTTTACATCCATTAAATCATTACAACAGCTAGTTGAAAACGTGACAACGTCACTGAGGCAATCATGAAACCACTTTCATCCGCAATAGCAGCTGCGCTTATTCTCTTTTC  >  NC_000913/1617874‑1618059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`