Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,633,590 A→G G94G (GGT→GGC)  b1547 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,633,5900AG100.0% 765.2 / NA 243G94G (GGT→GGC) b1547hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (128/115);  total (128/115)

Only 100 of 248 total aligned reads displayed.

AAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGA  >  NC_000913/1633500‑1633679
                                                                                          |                                                                                         
   tttCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                      <  1:3589521/93‑1 (MQ=17)
   tttCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                      <  2:4748481/93‑1 (MQ=18)
    ttCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACt                                                                                     <  2:3481796/93‑1 (MQ=17)
     tCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTg                                                                                    <  1:5468845/93‑1 (MQ=18)
     tCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTg                                                                                    <  2:2951205/93‑1 (MQ=17)
     tCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTg                                                                                    <  1:268434/93‑1 (MQ=18)
      cGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTgg                                                                                   >  1:4862189/1‑93 (MQ=18)
       gggTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGa                                                                                  <  2:981663/93‑1 (MQ=18)
         gTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATa                                                                                <  1:9612731/93‑1 (MQ=37)
         gTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATa                                                                                <  1:4522214/93‑1 (MQ=37)
         gTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATa                                                                                >  2:2781695/1‑93 (MQ=255)
          tATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaa                                                                               >  2:8496673/1‑93 (MQ=255)
           aTGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaaa                                                                              >  1:8511990/1‑93 (MQ=255)
            tGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATaaaa                                                                             >  1:5312586/1‑93 (MQ=255)
              cAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAAct                                                                           >  1:6278711/1‑93 (MQ=255)
                gATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAActct                                                                         >  2:7075544/1‑93 (MQ=255)
                gATTTGTCAAAAGTCTGCCACTGCATCAGGGCATAACCAGACGGAACGGCATCTGATGGCGACGGGATTGGTGCGCCGACTGGATAAAActct                                                                         >  2:1847071/1‑93 (MQ=14)
                 aTTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTg                                                                        >  1:7524800/1‑93 (MQ=255)
                  tttGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGc                                                                       <  2:5633277/93‑1 (MQ=255)
                  tttGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGc                                                                       <  1:7024669/93‑1 (MQ=255)
                      tCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCagga                                                                   >  2:9576227/1‑93 (MQ=255)
                       cAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCaggag                                                                  <  1:4542545/93‑1 (MQ=255)
                        aaaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTTGTGCGCCGACTGGATAAAACTCTGCaggagg                                                                 >  1:9053357/1‑93 (MQ=255)
                        aaaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCaggagg                                                                 <  1:6191523/93‑1 (MQ=255)
                         aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAggagga                                                                >  1:2913870/1‑93 (MQ=255)
                         aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAggagga                                                                >  1:7848444/1‑93 (MQ=255)
                         aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAggagga                                                                <  1:4946567/93‑1 (MQ=255)
                         aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAggagga                                                                <  2:4664104/93‑1 (MQ=255)
                         aaaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAggagga                                                                <  2:8414824/93‑1 (MQ=255)
                          aaGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAt                                                               <  2:2733370/93‑1 (MQ=255)
                            gTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGa                                                             <  1:438538/93‑1 (MQ=255)
                             tCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAg                                                            >  2:1251161/1‑93 (MQ=255)
                              cTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGc                                                           <  1:2346320/93‑1 (MQ=255)
                              cTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGc                                                           <  2:1499527/93‑1 (MQ=255)
                               tGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGcc                                                          >  2:8682926/1‑93 (MQ=255)
                                gCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCg                                                         >  1:1885459/1‑93 (MQ=255)
                                 ccccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGa                                                        <  1:9368722/93‑1 (MQ=255)
                                  cccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAg                                                       >  1:156751/1‑93 (MQ=255)
                                  cccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAg                                                       >  2:2168875/1‑93 (MQ=255)
                                   ccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAgg                                                      >  2:2248291/1‑93 (MQ=255)
                                   ccTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAgg                                                      >  2:5997838/1‑93 (MQ=255)
                                    cTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgt                                                     >  1:995923/1‑93 (MQ=255)
                                    cTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgt                                                     <  1:1026486/93‑1 (MQ=255)
                                     tGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgtg                                                    >  1:8377686/1‑93 (MQ=255)
                                     tGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgtg                                                    >  2:4195759/1‑93 (MQ=255)
                                     tGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGgtg                                                    >  2:4500432/1‑93 (MQ=255)
                                       cATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTa                                                  <  1:3280528/93‑1 (MQ=255)
                                        aTCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaa                                                 <  2:9661169/93‑1 (MQ=255)
                                        aTCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaa                                                 <  1:3240498/93‑1 (MQ=255)
                                        aTCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaa                                                 >  2:8106448/1‑93 (MQ=255)
                                        aTCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaa                                                 >  1:2576250/1‑93 (MQ=255)
                                         tCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaaa                                                <  1:7618224/93‑1 (MQ=255)
                                         tCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTaaa                                                >  1:5149273/1‑93 (MQ=255)
                                           aGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGc                                              >  1:6164552/1‑93 (MQ=255)
                                              gCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgc                                           <  2:4996585/93‑1 (MQ=255)
                                               cATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgcg                                          >  1:5627336/1‑93 (MQ=255)
                                               cATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTgcg                                          <  2:1646227/93‑1 (MQ=255)
                                                 tAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGcc                                        >  2:6104077/1‑93 (MQ=255)
                                                 tAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGcc                                        >  2:7751144/1‑93 (MQ=255)
                                                 tAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGcc                                        >  1:8131472/1‑93 (MQ=255)
                                                 tAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGcc                                        >  2:7787628/1‑93 (MQ=255)
                                                  aaCCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGccc                                       >  2:3382587/1‑93 (MQ=255)
                                                   aCCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCa                                      >  1:5524023/1‑93 (MQ=255)
                                                   aCCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCa                                      <  1:4010962/93‑1 (MQ=255)
                                                   aCCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCa                                      >  2:5195842/1‑93 (MQ=255)
                                                    ccAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCAc                                     <  1:4269138/93‑1 (MQ=255)
                                                    acAGACGGAACGGTATCTGGTGGTCACGGGATTGGGGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTTTAAAGCTGCGCCCAc                                     <  2:6885388/92‑1 (MQ=25)
                                                     cAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACg                                    <  2:37796/93‑1 (MQ=255)
                                                      aGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATTAGCCGAGGTGTAAAGCTGCGCCCACgg                                   >  1:3703556/1‑93 (MQ=255)
                                                      aGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACgg                                   <  1:3126697/93‑1 (MQ=255)
                                                       gACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAACCTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGc                                  <  1:3922951/93‑1 (MQ=255)
                                                       gACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGc                                  >  2:6785220/1‑93 (MQ=255)
                                                         cGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGa                                >  2:763734/1‑93 (MQ=255)
                                                         cGGAACGGTATATGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGa                                <  1:4679157/93‑1 (MQ=255)
                                                          ggAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAc                               <  1:4728984/93‑1 (MQ=255)
                                                          ggAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAc                               <  2:1620291/93‑1 (MQ=255)
                                                            aaCGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCa                             >  1:6561192/1‑93 (MQ=255)
                                                             aCGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCACCCACTGCGAcacg                            >  1:1774801/1‑90 (MQ=37)
                                                              cGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAACCTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGAGCCCACGGCGAccgga                           <  2:8992415/93‑4 (MQ=37)
                                                               ggTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGtt                          >  1:1152201/1‑93 (MQ=255)
                                                                gTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAACGCTGCGCCCACGGCGACCAGttt                         >  2:5506688/1‑93 (MQ=255)
                                                                 tATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTg                        >  2:9323233/1‑93 (MQ=255)
                                                                 tATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTg                        >  1:8282207/1‑93 (MQ=255)
                                                                  aTCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGc                       <  1:7269950/93‑1 (MQ=255)
                                                                   tCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCg                      <  2:6350196/93‑1 (MQ=255)
                                                                   tCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCg                      <  1:2459583/93‑1 (MQ=255)
                                                                   tCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCg                      <  2:9186667/93‑1 (MQ=255)
                                                                        tGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggt                 <  2:9870082/93‑1 (MQ=255)
                                                                        tGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggt                 <  1:8842764/93‑1 (MQ=255)
                                                                        tGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggt                 <  1:1099317/93‑1 (MQ=255)
                                                                          gCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtcg               >  2:73992/1‑93 (MQ=255)
                                                                            cACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTa             <  2:5714027/93‑1 (MQ=255)
                                                                            cACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTa             <  2:9499755/93‑1 (MQ=255)
                                                                              cGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATc           <  2:2098902/93‑1 (MQ=255)
                                                                               gggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATcc          <  1:991528/93‑1 (MQ=255)
                                                                                ggATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc         >  2:4055142/1‑93 (MQ=255)
                                                                                 gATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcg        <  2:2709520/93‑1 (MQ=255)
                                                                                  aTTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcgt       <  2:2672651/93‑1 (MQ=255)
                                                                                     ggTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCcgtcgt    <  2:2822549/93‑1 (MQ=255)
                                                                                      gTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTg   <  1:2025937/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGA  >  NC_000913/1633500‑1633679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAC  >  NC_000913/1633497‑1633681
                                                                                             |                                                                                           
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCG                                                                                             <  SRR4302540.2209761/94‑1 (MQ=23)
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGCATCTGATGGCCACGGGATTGGTGCG                                                                                             >  SRR4302540.1840084/1‑94 (MQ=22)
 GCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGC                                                                                            <  SRR4302540.897325/94‑1 (MQ=21)
   AAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCG                                                                                          <  SRR4302540.3446835/94‑1 (MQ=12)
     GTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAC                                                                                        <  SRR4302540.4496283/94‑1 (MQ=10)
      TTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACT                                                                                       <  SRR4302540.3628731/94‑1 (MQ=10)
        TCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGG                                                                                     <  SRR4302540.3311104/94‑1 (MQ=10)
        TCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGG                                                                                     <  SRR4302540.9257775/94‑1 (MQ=10)
        TCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGG                                                                                     >  SRR4302540.4914332/1‑94 (MQ=10)
        TCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGG                                                                                     <  SRR4302540.271082/94‑1 (MQ=10)
        TCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGG                                                                                     <  SRR4302540.5529041/94‑1 (MQ=10)
        TCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCAGCAGGGCATAACCAGACGGACAGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGG                                                                                     <  SRR4302540.5529042/94‑1 (MQ=8)
         CGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGA                                                                                    >  SRR4302540.10073110/1‑94 (MQ=12)
            GTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAA                                                                                 <  SRR4302540.9726073/94‑1 (MQ=20)
            GTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAA                                                                                 <  SRR4302540.9726069/94‑1 (MQ=20)
            GTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAA                                                                                 <  SRR4302540.7642766/94‑1 (MQ=20)
            GTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAA                                                                                 <  SRR4302540.4571188/94‑1 (MQ=20)
             TATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAA                                                                                >  SRR4302540.8610917/1‑94 (MQ=20)
              ATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAA                                                                               >  SRR4302540.5370750/1‑94 (MQ=20)
               TGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAAC                                                                              <  SRR4302540.8516251/94‑1 (MQ=20)
                GCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACT                                                                             >  SRR4302540.6348578/1‑94 (MQ=20)
                 CAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTC                                                                            >  SRR4302540.372026/1‑94 (MQ=23)
                  AGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCT                                                                           >  SRR4302540.3216263/1‑94 (MQ=25)
                  AGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCT                                                                           <  SRR4302540.8477543/94‑1 (MQ=25)
                   GATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTG                                                                          >  SRR4302540.7608993/1‑94 (MQ=28)
                     TTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCA                                                                        <  SRR4302540.4192881/94‑1 (MQ=33)
                     TTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCA                                                                        <  SRR4302540.7102702/94‑1 (MQ=33)
                      TTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAG                                                                       <  SRR4302540.5001498/94‑1 (MQ=35)
                       TGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGG                                                                      >  SRR4302540.2451716/1‑94 (MQ=38)
                        GTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGA                                                                     >  SRR4302540.6272487/1‑94 (MQ=40)
                        GTCAAAAGTCTGCCCCTGCACCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGA                                                                     <  SRR4302540.4573424/94‑1 (MQ=39)
                         TCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAG                                                                    <  SRR4302540.8285987/94‑1 (MQ=43)
                          CAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTTGTGCGCCGACTGGATAAAACTCTGCAGGAGG                                                                   >  SRR4302540.9162037/1‑94 (MQ=43)
                          CAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGG                                                                   <  SRR4302540.4591704/94‑1 (MQ=45)
                           AAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGA                                                                  <  SRR4302540.6260455/94‑1 (MQ=48)
                           AAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGA                                                                  >  SRR4302540.2945739/1‑94 (MQ=48)
                           AAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGA                                                                  >  SRR4302540.8304958/1‑94 (MQ=48)
                           AAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGA                                                                  >  SRR4302540.7940151/1‑94 (MQ=48)
                            AAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAT                                                                 <  SRR4302540.4999974/94‑1 (MQ=50)
                            AAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGAT                                                                 >  SRR4302540.378387/1‑94 (MQ=50)
                             AAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATG                                                                >  SRR4302540.431530/1‑94 (MQ=50)
                              AGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGA                                                               >  SRR4302540.7400442/1‑94 (MQ=50)
                               GTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAG                                                              <  SRR4302540.443196/94‑1 (MQ=50)
                                 CTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCG                                                            >  SRR4302540.7806794/1‑94 (MQ=47)
                                 CTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCC                                                            >  SRR4302540.8413773/1‑94 (MQ=50)
                                 CTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCC                                                            <  SRR4302540.2371597/94‑1 (MQ=50)
                                  TGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCG                                                           >  SRR4302540.8881053/1‑94 (MQ=53)
                                  TGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCG                                                           >  SRR4302540.1906104/1‑94 (MQ=53)
                                  TGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCG                                                           <  SRR4302540.2897577/94‑1 (MQ=53)
                                    CCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAG                                                         <  SRR4302540.9480268/94‑1 (MQ=58)
                                    CCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAG                                                         >  SRR4302540.158342/1‑94 (MQ=58)
                                     CCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGCTAAAACTCTGCAGGAGGATGAGCCGAGG                                                        >  SRR4302540.1547552/1‑94 (MQ=46)
                                     CCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGG                                                        >  SRR4302540.6824843/1‑94 (MQ=60)
                                      CCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGT                                                       >  SRR4302540.1006063/1‑94 (MQ=60)
                                      CCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGT                                                       <  SRR4302540.9003901/94‑1 (MQ=60)
                                       CTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTG                                                      >  SRR4302540.8474901/1‑94 (MQ=60)
                                       CTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTG                                                      >  SRR4302540.3989006/1‑94 (MQ=60)
                                       CTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTG                                                      >  SRR4302540.2500463/1‑94 (MQ=60)
                                       CTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTG                                                      <  SRR4302540.1036897/94‑1 (MQ=60)
                                        TGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGT                                                     >  SRR4302540.6159412/1‑94 (MQ=60)
                                        TGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGT                                                     <  SRR4302540.1894403/94‑1 (MQ=60)
                                          CATCAGGGCCTAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATCAACCTCTGCAGGAGGATGAGCCGAGGTGTAA                                                   >  SRR4302540.7173441/1‑94 (MQ=42)
                                          CATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAA                                                   <  SRR4302540.3316366/94‑1 (MQ=60)
                                          CATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAA                                                   >  SRR4302540.2604020/1‑94 (MQ=60)
                                          CATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGGGTAA                                                   >  SRR4302540.2604021/1‑94 (MQ=58)
                                           ATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAA                                                  <  SRR4302540.3275761/94‑1 (MQ=60)
                                           ATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAA                                                  >  SRR4302540.5205102/1‑94 (MQ=60)
                                            TCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAG                                                 >  SRR4302540.6255532/1‑94 (MQ=60)
                                            TCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAG                                                 <  SRR4302540.7703878/94‑1 (MQ=60)
                                             CAGGGCATAACCAGCCGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGgctcaggt                                                >  SRR4302540.6233240/1‑86 (MQ=60)
                                             CAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC                                                <  SRR4302540.2240075/94‑1 (MQ=60)
                                             CAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC                                                >  SRR4302540.6233239/1‑94 (MQ=60)
                                             CAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC                                                <  SRR4302540.4635977/94‑1 (MQ=60)
                                             CAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGC                                                <  SRR4302540.629471/94‑1 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                               >  SRR4302540.4893071/1‑94 (MQ=60)
                                              AGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCT                                               >  SRR4302540.7462916/1‑94 (MQ=60)
                                               GGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTG                                              <  SRR4302540.1917321/94‑1 (MQ=60)
                                                 GCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCG                                            >  SRR4302540.5689275/1‑94 (MQ=60)
                                                   ATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCC                                          >  SRR4302540.2948410/1‑94 (MQ=60)
                                                   ATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCC                                          <  SRR4302540.4758707/94‑1 (MQ=60)
                                                   ATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCC                                          >  SRR4302540.8951067/1‑94 (MQ=60)
                                                   ATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCC                                          >  SRR4302540.8225859/1‑94 (MQ=60)
                                                   ATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCC                                          >  SRR4302540.7016575/1‑94 (MQ=60)
                                                    TAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCC                                         <  SRR4302540.9596834/94‑1 (MQ=60)
                                                    TAACCAGACGGAACGGTATCTGATGGACACGGGATTGGTGCGCCGACTGGATAAACCTCTGCAGGAGGATGAGCCGAGTTGTAAAGCTGCGCCC                                         <  SRR4302540.7786411/94‑1 (MQ=60)
                                                     AACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCA                                        <  SRR4302540.10069382/94‑1 (MQ=60)
                                                     AACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCA                                        >  SRR4302540.5584850/1‑94 (MQ=60)
                                                      ACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCAC                                       <  SRR4302540.4054164/94‑1 (MQ=60)
                                                      ACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCCGCAGGAGGATGAGCCGAGGTGTAAAGCTTCGCCCAC                                       <  SRR4302540.4184678/94‑1 (MQ=60)
                                                       CCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACG                                      <  SRR4302540.4315573/94‑1 (MQ=60)
                                                        CAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATTAGCCGAGGTGTAAAGCTGCGCCCACGG                                     >  SRR4302540.3743989/1‑94 (MQ=60)
                                                         AGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGC                                    <  SRR4302540.5643086/94‑1 (MQ=60)
                                                         AGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGC                                    <  SRR4302540.3160837/94‑1 (MQ=60)
                                                          GACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAACCTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCG                                   <  SRR4302540.3965406/94‑1 (MQ=60)
                                                            CGGAACGGTATATGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGAC                                 <  SRR4302540.4729221/94‑1 (MQ=60)
                                                             GGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACC                                <  SRR4302540.4779485/94‑1 (MQ=60)
                                                              GAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCA                               >  SRR4302540.6633927/1‑94 (MQ=60)
                                                               AACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAG                              >  SRR4302540.1873071/1‑94 (MQ=60)
                                                               AACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCACCCACTGCGACacg                              >  SRR4302540.1794075/1‑91 (MQ=60)
                                                                ACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGT                             >  SRR4302540.5572313/1‑94 (MQ=60)
                                                                ACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGT                             >  SRR4302540.4674633/1‑94 (MQ=60)
                                                                 CGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTT                            >  SRR4302540.1771454/1‑94 (MQ=60)
                                                                 CGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTT                            >  SRR4302540.1164204/1‑94 (MQ=60)
                                                                  GGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTT                           <  SRR4302540.811573/94‑1 (MQ=60)
                                                                  GGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTT                           <  SRR4302540.551692/94‑1 (MQ=60)
                                                                   GTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTG                          >  SRR4302540.4553004/1‑94 (MQ=60)
                                                                   GTATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTG                          >  SRR4302540.8378402/1‑94 (MQ=60)
                                                                    TATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGC                         >  SRR4302540.5534569/1‑94 (MQ=60)
                                                                     ATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCG                        >  SRR4302540.950897/1‑94 (MQ=60)
                                                                     ATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCG                        <  SRR4302540.7351072/94‑1 (MQ=60)
                                                                     ATCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCG                        <  SRR4302540.3449892/94‑1 (MQ=60)
                                                                      TCTGATGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGT                       <  SRR4302540.2486047/94‑1 (MQ=60)
                                                                        ctatgggCACGGGGTTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCG                     <  SRR4302540.2582038/87‑1 (MQ=60)
                                                                           TGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTC                  <  SRR4302540.6538841/94‑1 (MQ=60)
                                                                           TGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTC                  <  SRR4302540.1110638/94‑1 (MQ=60)
                                                                           TGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTC                  <  SRR4302540.8945052/94‑1 (MQ=60)
                                                                           TGGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTC                  <  SRR4302540.1110639/94‑1 (MQ=60)
                                                                            GGCCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCG                 >  SRR4302540.2779705/1‑94 (MQ=60)
                                                                              CCACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGAAAAGCTCCGCCCACGGCGACCAGTTTGAGTCGGTCGgt               >  SRR4302540.1497902/1‑92 (MQ=60)
                                                                               CACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAT              <  SRR4302540.4979005/94‑1 (MQ=60)
                                                                               CACGGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAT              <  SRR4302540.1115041/94‑1 (MQ=60)
                                                                               CACGGAATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTAT              >  SRR4302540.3763466/1‑94 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC           >  SRR4302540.3209286/1‑94 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC           <  SRR4302540.1001644/94‑1 (MQ=60)
                                                                                  GGGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCC           <  SRR4302540.6045038/94‑1 (MQ=60)
                                                                                   GGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCG          <  SRR4302540.5236688/94‑1 (MQ=60)
                                                                                   GGATTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCG          <  SRR4302540.5280881/94‑1 (MQ=60)
                                                                                      TTGGTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCG       <  SRR4302540.483037/94‑1 (MQ=60)
                                                                                         GTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGTGACCAGTTTGCGTCGGTCGTATCCCGTCGTGA    >  SRR4302540.2051039/1‑94 (MQ=60)
                                                                                         GTGCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGA    <  SRR4302540.2047821/94‑1 (MQ=60)
                                                                                           GCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAC  <  SRR4302540.8960946/94‑1 (MQ=60)
                                                                                           GCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAC  <  SRR4302540.2446983/94‑1 (MQ=60)
                                                                                           GCGCCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAC  >  SRR4302540.9878601/1‑94 (MQ=60)
                                                                                             |                                                                                           
TGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAAC  >  NC_000913/1633497‑1633681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑