Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,656,923 T→C P277P (CCT→CCC)  b1587 → putative oxidoreductase major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,656,9230TC99.6% 813.7 / ‑4.9 252P277P (CCT→CCC) b1587putative oxidoreductase major subunit
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (150/101);  total (150/102)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 259 total aligned reads displayed.

AAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAA  >  NC_000913/1656833‑1657012
                                                                                          |                                                                                         
aaaaGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCAt                                                                                         <  2:2731755/93‑1 (MQ=255)
 aaaGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCAtt                                                                                        >  1:4161438/1‑93 (MQ=255)
 aaaGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCAtt                                                                                        <  1:1438553/93‑1 (MQ=255)
   aGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCg                                                                                      >  2:6073329/1‑93 (MQ=255)
   aGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCg                                                                                      >  2:301292/1‑93 (MQ=255)
     cgcgcgAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGcc                                                                                    >  2:156567/1‑93 (MQ=255)
       cgcgAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCg                                                                                  >  1:4634540/1‑93 (MQ=255)
       cgcgAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCg                                                                                  >  2:8088012/1‑93 (MQ=255)
           agaAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCa                                                                              >  1:1509798/1‑93 (MQ=255)
           agaAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCa                                                                              >  1:3297333/1‑93 (MQ=255)
            gaAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCAc                                                                             >  1:1632802/1‑93 (MQ=255)
            gaAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCAc                                                                             >  1:8190012/1‑93 (MQ=255)
              aaTCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCg                                                                           >  2:3595844/1‑93 (MQ=255)
              aaTCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCg                                                                           >  2:4690975/1‑93 (MQ=255)
                tCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGAt                                                                         >  1:3727325/1‑93 (MQ=255)
                acGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGAt                                                                         <  2:8131685/92‑1 (MQ=255)
                    aCGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCcg                                                                     <  1:2034714/93‑1 (MQ=255)
                    aCGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCcg                                                                     >  2:8304505/1‑93 (MQ=255)
                    aCGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCcg                                                                     >  2:6157158/1‑93 (MQ=255)
                     cGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCcgc                                                                    <  1:6417394/93‑1 (MQ=255)
                     cGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCcgc                                                                    >  2:5086107/1‑93 (MQ=255)
                       ccAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCCCCGATGCCgcgc                                                                  >  2:1671859/1‑93 (MQ=255)
                        cAAAATTATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCt                                                                 <  2:4086136/93‑1 (MQ=255)
                          aaaTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTgg                                                               <  2:227397/93‑1 (MQ=255)
                          aaaTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTgg                                                               <  2:525797/93‑1 (MQ=255)
                          aaaTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCAACGATGCCGCGCTgg                                                               >  2:8856646/1‑93 (MQ=255)
                           aaTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGt                                                              >  1:9873381/1‑93 (MQ=255)
                            aTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCTATTCGCCCGGGCACCGATGCCGCGCTGGtt                                                             <  2:2904104/93‑1 (MQ=255)
                            aTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGtt                                                             >  2:1416051/1‑93 (MQ=255)
                            aTGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGtt                                                             >  2:8562405/1‑93 (MQ=255)
                             tGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTg                                                            >  2:6672582/1‑93 (MQ=255)
                             tGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTg                                                            >  2:1164063/1‑93 (MQ=255)
                               aTTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCg                                                          <  1:10046184/93‑1 (MQ=255)
                                ttGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCgg                                                         >  1:4514280/1‑93 (MQ=255)
                                ttGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCgg                                                         >  1:8249975/1‑93 (MQ=255)
                                 tGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCggg                                                        >  2:3178981/1‑93 (MQ=255)
                                  gTTATCGCTCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGt                                                       <  2:8924391/93‑1 (MQ=255)
                                  gTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGt                                                       >  1:4318712/1‑93 (MQ=255)
                                  gTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGt                                                       >  1:9125789/1‑93 (MQ=255)
                                   ttATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTa                                                      <  1:4028678/93‑1 (MQ=255)
                                    tATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGGTGCGGGTAt                                                     >  2:387247/1‑93 (MQ=255)
                                     accGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTAtt                                                    <  2:4358873/91‑1 (MQ=255)
                                     aTCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTAtt                                                    <  1:2192163/93‑1 (MQ=255)
                                      tCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTg                                                   >  1:4097452/1‑93 (MQ=255)
                                        gATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTccc                                                 >  1:8761282/1‑93 (MQ=255)
                                        gATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGcc                                                 <  2:650780/93‑1 (MQ=255)
                                         aTCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCt                                                <  1:7399649/93‑1 (MQ=255)
                                         aTCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCt                                                <  1:8439308/93‑1 (MQ=255)
                                          tCCGCGATATACCGATACGACTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTg                                               <  1:4994952/93‑1 (MQ=255)
                                               gATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAt                                          >  1:2476551/1‑93 (MQ=255)
                                                atatACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAtt                                         >  1:6306385/1‑93 (MQ=255)
                                                atatACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAtt                                         >  1:9499944/1‑93 (MQ=255)
                                                 tatcCCGATCCGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTg                                        <  1:5459678/93‑1 (MQ=255)
                                                 tataCCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCCCCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTg                                        >  1:8478063/1‑93 (MQ=255)
                                                 tataCCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTg                                        <  1:6322824/93‑1 (MQ=255)
                                                   taCCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGAt                                      >  2:7706787/1‑93 (MQ=255)
                                                   taCCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGACGCGCTGGTTGCGGGTATTGCCTGGGTATTGAt                                      >  1:7228475/1‑93 (MQ=255)
                                                   gaCCGATACGGCTGCCGGTCGGGAAGACGAAAGGCGCCCCATTCGCCCGGGCACCGATGCCGCGCCGGTGGCGGGGATTGCCGGGGGATTGAt                                      >  1:7695146/2‑93 (MQ=255)
                                                    aCCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGAtt                                     <  2:7817200/93‑1 (MQ=255)
                                                    aCCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGGATTGCCCGGGTATTGAtt                                     >  1:4072071/1‑93 (MQ=255)
                                                     ccGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTa                                    >  1:9592535/1‑93 (MQ=255)
                                                      cGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTaa                                   <  2:4044863/93‑1 (MQ=255)
                                                      cGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTaa                                   >  1:3697236/1‑93 (MQ=255)
                                                        aTACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAAca                                 >  2:4881219/1‑92 (MQ=255)
                                                        aTACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACg                                 >  1:1043462/1‑93 (MQ=255)
                                                        aTACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACg                                 <  2:5220179/93‑1 (MQ=255)
                                                         tACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGa                                >  1:10020626/1‑93 (MQ=255)
                                                         tACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGa                                <  1:5116733/93‑1 (MQ=255)
                                                         tACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGa                                >  2:726961/1‑93 (MQ=255)
                                                          aCGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGaa                               >  1:6761420/1‑93 (MQ=255)
                                                              cTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAAtc                           <  2:2320014/93‑1 (MQ=255)
                                                                 ccGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCg                        <  2:8633976/93‑1 (MQ=255)
                                                                 ccGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCg                        >  1:1140948/1‑93 (MQ=255)
                                                                  cGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGt                       >  1:9205544/1‑93 (MQ=255)
                                                                  cGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGt                       <  2:5656358/93‑1 (MQ=255)
                                                                  cGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGt                       <  1:4772070/93‑1 (MQ=255)
                                                                   ggTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGtt                      <  2:3765287/93‑1 (MQ=255)
                                                                    gTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTg                     >  1:2945977/1‑93 (MQ=255)
                                                                     tCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGa                    <  1:9747079/93‑1 (MQ=255)
                                                                     tCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGa                    >  2:4933357/1‑93 (MQ=255)
                                                                     tCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGa                    <  2:5416915/93‑1 (MQ=255)
                                                                      cGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGAt                   >  2:7973824/1‑93 (MQ=255)
                                                                       gTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATc                  <  2:214260/93‑1 (MQ=255)
                                                                       gTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGGATTGCCTGGGGATTGATTAACGAAAATCTCGTTGCTc                  >  1:527776/1‑93 (MQ=255)
                                                                        tGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCa                 >  1:2355840/1‑93 (MQ=255)
                                                                        tGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCa                 >  2:3212971/1‑93 (MQ=255)
                                                                          aaGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAAc               <  2:1305185/93‑1 (MQ=255)
                                                                          aaGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAAc               >  2:683147/1‑93 (MQ=255)
                                                                          aaGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAAc               >  2:7495164/1‑93 (MQ=255)
                                                                          aaGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAAc               <  1:3551027/93‑1 (MQ=255)
                                                                          aaGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAAc               >  1:4872826/1‑93 (MQ=255)
                                                                            gACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCt             >  1:6068235/1‑93 (MQ=255)
                                                                              cGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCttt           <  2:6450938/93‑1 (MQ=255)
                                                                              cGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCttt           <  1:6235878/93‑1 (MQ=255)
                                                                              cGAATGGCGCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCttt           <  2:1860852/93‑1 (MQ=255)
                                                                               gAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCtttt          >  1:6874815/1‑93 (MQ=255)
                                                                                  tGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTtctc       <  2:10047020/93‑1 (MQ=255)
                                                                                  tGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGCATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTtctc       >  1:5856508/1‑93 (MQ=255)
                                                                                      tCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATa   >  1:5917220/1‑93 (MQ=255)
                                                                                       ccccATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATaa  >  2:9726677/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAA  >  NC_000913/1656833‑1657012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATAC  >  NC_000913/1656832‑1657016
                                                                                           |                                                                                             
AAAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCAT                                                                                             <  SRR4302540.6260639/94‑1 (MQ=60)
 AAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATT                                                                                            >  SRR4302540.4206544/1‑94 (MQ=60)
  AAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTC                                                                                           <  SRR4302540.1454163/94‑1 (MQ=60)
    AGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGC                                                                                         <  SRR4302540.7598425/94‑1 (MQ=60)
     GCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCC                                                                                        <  SRR4302540.5526804/94‑1 (MQ=60)
       GCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCG                                                                                      >  SRR4302540.4684161/1‑94 (MQ=60)
        CGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGG                                                                                     <  SRR4302540.7143119/94‑1 (MQ=60)
           GAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCA                                                                                  >  SRR4302540.3333422/1‑94 (MQ=60)
           GAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCA                                                                                  >  SRR4302540.1526481/1‑94 (MQ=60)
           GAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCA                                                                                  >  SRR4302540.1526480/1‑94 (MQ=60)
            AGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCAC                                                                                 >  SRR4302540.1650665/1‑94 (MQ=60)
            AGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCAC                                                                                 >  SRR4302540.8285177/1‑94 (MQ=60)
              AAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCG                                                                               <  SRR4302540.9436143/94‑1 (MQ=60)
              AAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCG                                                                               <  SRR4302540.4586/94‑1 (MQ=60)
                ATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGAT                                                                             >  SRR4302540.3767990/1‑94 (MQ=60)
                ATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGAG                                                                             >  SRR4302540.3767708/1‑94 (MQ=60)
                 TCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATG                                                                            >  SRR4302540.6714209/1‑94 (MQ=60)
                  CGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGC                                                                           >  SRR4302540.2443514/1‑94 (MQ=60)
                     ACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGC                                                                        <  SRR4302540.2056657/94‑1 (MQ=60)
                      CGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCG                                                                       <  SRR4302540.6488823/94‑1 (MQ=60)
                      CGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCG                                                                       <  SRR4302540.5081718/94‑1 (MQ=60)
                         CAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTG                                                                    >  SRR4302540.1116265/1‑94 (MQ=60)
                          AAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGG                                                                   <  SRR4302540.6097566/94‑1 (MQ=60)
                          AAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGG                                                                   >  SRR4302540.9265203/1‑94 (MQ=60)
                           AAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGT                                                                  >  SRR4302540.9988687/1‑94 (MQ=60)
                           AAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGT                                                                  >  SRR4302540.6897853/1‑94 (MQ=60)
                           AAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCACTGGT                                                                  >  SRR4302540.6473188/1‑94 (MQ=60)
                            AATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTT                                                                 <  SRR4302540.5100780/94‑1 (MQ=60)
                            AATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTT                                                                 <  SRR4302540.1018307/94‑1 (MQ=60)
                            AATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTT                                                                 <  SRR4302540.4090284/94‑1 (MQ=60)
                             ATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTG                                                                <  SRR4302540.4423356/94‑1 (MQ=60)
                               GATTTGTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCG                                                              <  SRR4302540.1176142/94‑1 (MQ=60)
                               GATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGTGTGCG                                                              >  SRR4302540.5209538/1‑94 (MQ=60)
                                ATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGG                                                             >  SRR4302540.4563189/1‑94 (MQ=60)
                                ATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGG                                                             >  SRR4302540.8345870/1‑94 (MQ=60)
                                ATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGG                                                             <  SRR4302540.10162969/94‑1 (MQ=60)
                                ATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGG                                                             <  SRR4302540.2280090/94‑1 (MQ=60)
                                 TTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGG                                                            >  SRR4302540.6356450/1‑94 (MQ=60)
                                 TTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGG                                                            >  SRR4302540.6356452/1‑94 (MQ=60)
                                  TGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGT                                                           >  SRR4302540.4365724/1‑94 (MQ=60)
                                  TGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGT                                                           >  SRR4302540.9235481/1‑94 (MQ=60)
                                  TGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGT                                                           >  SRR4302540.2233893/1‑94 (MQ=60)
                                   GTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTA                                                          >  SRR4302540.6051427/1‑94 (MQ=60)
                                    TTATCGATCCGGCATTTCCCGATACCGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTAT                                                         <  SRR4302540.4072122/94‑1 (MQ=60)
                                    TTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTAT                                                         >  SRR4302540.8146613/1‑94 (MQ=60)
                                    TTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTAT                                                         <  SRR4302540.4072121/94‑1 (MQ=60)
                                    TTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTAT                                                         <  SRR4302540.4160123/94‑1 (MQ=60)
                                     TATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATT                                                        >  SRR4302540.4245466/1‑94 (MQ=60)
                                      ATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTG                                                       >  SRR4302540.4141652/1‑94 (MQ=60)
                                      ATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTG                                                       <  SRR4302540.2215838/94‑1 (MQ=60)
                                       ACGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGC                                                      <  SRR4302540.2945720/94‑1 (MQ=60)
                                        CGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCC                                                     >  SRR4302540.8407251/1‑94 (MQ=60)
                                        CGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTCCC                                                     >  SRR4302540.8862869/1‑94 (MQ=60)
                                         GATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCT                                                    <  SRR4302540.8883269/94‑1 (MQ=60)
                                          ATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTG                                                   <  SRR4302540.7482086/94‑1 (MQ=60)
                                          ATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTG                                                   <  SRR4302540.8537274/94‑1 (MQ=60)
                                           TCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGG                                                  <  SRR4302540.9794314/94‑1 (MQ=60)
                                           TCCGCGATATACCGATACGACTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGG                                                  <  SRR4302540.5049024/94‑1 (MQ=60)
                                            CCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGG                                                 >  SRR4302540.448280/1‑94 (MQ=60)
                                              CCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTA                                               <  SRR4302540.9919864/94‑1 (MQ=60)
                                               CGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAT                                              >  SRR4302540.2503216/1‑94 (MQ=60)
                                                GATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATT                                             >  SRR4302540.6376616/1‑94 (MQ=60)
                                                GATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATT                                             >  SRR4302540.5370626/1‑94 (MQ=60)
                                                GATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATT                                             >  SRR4302540.9612518/1‑94 (MQ=60)
                                                GATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATT                                             >  SRR4302540.6376617/1‑94 (MQ=60)
                                                GATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATT                                             >  SRR4302540.851668/1‑94 (MQ=60)
                                                GATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATT                                             >  SRR4302540.414868/1‑94 (MQ=60)
                                                 ATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCCCCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTG                                            >  SRR4302540.8576535/1‑94 (MQ=60)
                                                  TATCCCGATCCGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGA                                           <  SRR4302540.5519768/94‑1 (MQ=60)
                                                  TATACCGATACGGCTGCCGGTTGTGAACACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCATGGGAATTGG                                           >  SRR4302540.8621597/1‑94 (MQ=60)
                                                  TATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGTGGGTATTGCCTGGGTATTGA                                           >  SRR4302540.1016625/1‑94 (MQ=60)
                                                  TATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGA                                           <  SRR4302540.6393236/94‑1 (MQ=60)
                                                  TATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGA                                           <  SRR4302540.6393237/94‑1 (MQ=60)
                                                   ATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGAT                                          <  SRR4302540.3697271/94‑1 (MQ=60)
                                                   ATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGACGCGCTGGTTGCGGGTATTGCCTGGGTATTGAT                                          >  SRR4302540.7309083/1‑94 (MQ=60)
                                                   AGACCGATACGGCTGCCGGTCGGGAAGACGAAAGGCGCCCCATTCGCCCGGGCACCGATGCCGCGCCGGTGGCGGGGATTGCCGGGGGATTGAT                                          >  SRR4302540.7782804/1‑94 (MQ=48)
                                                    TACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCTCTGGTTGCGGGTATTGCCTGGGTATTGATT                                         <  SRR4302540.9961244/94‑1 (MQ=60)
                                                    TACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGGATTGCCCGGGTATTGATT                                         >  SRR4302540.4115966/1‑94 (MQ=60)
                                                     ACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTA                                        >  SRR4302540.6655023/1‑94 (MQ=60)
                                                     ACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTA                                        >  SRR4302540.9705739/1‑94 (MQ=60)
                                                      CCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAA                                       >  SRR4302540.3737599/1‑94 (MQ=60)
                                                      CCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAA                                       >  SRR4302540.4808237/1‑94 (MQ=60)
                                                        TATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACG                                     >  SRR4302540.1054068/1‑94 (MQ=60)
                                                        GATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACG                                     <  SRR4302540.150871/94‑1 (MQ=60)
                                                        GATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACG                                     >  SRR4302540.3797433/1‑94 (MQ=60)
                                                         ATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGA                                    >  SRR4302540.10137246/1‑94 (MQ=60)
                                                          TACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAA                                   <  SRR4302540.5172124/94‑1 (MQ=60)
                                                          TACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAA                                   >  SRR4302540.6836145/1‑94 (MQ=60)
                                                           ACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAA                                  <  SRR4302540.1985794/94‑1 (MQ=60)
                                                           ACGGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAA                                  >  SRR4302540.5837597/1‑94 (MQ=60)
                                                           ACGGCGGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAA                                  <  SRR4302540.6155249/94‑1 (MQ=60)
                                                             GGCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGGATTGCCTGGGGATTGATTAACGAAAAT                                >  SRR4302540.5926148/1‑94 (MQ=60)
                                                              GCTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATC                               <  SRR4302540.3675583/94‑1 (MQ=60)
                                                               GTGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCT                              <  SRR4302540.3110351/94‑1 (MQ=60)
                                                                TGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTC                             <  SRR4302540.3340610/94‑1 (MQ=60)
                                                                TGCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTC                             >  SRR4302540.5399390/1‑94 (MQ=60)
                                                                 GCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCG                            >  SRR4302540.3848480/1‑94 (MQ=60)
                                                                 GCCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCG                            >  SRR4302540.1152876/1‑94 (MQ=60)
                                                                  CCGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGT                           >  SRR4302540.9316002/1‑94 (MQ=60)
                                                                   CGGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTT                          <  SRR4302540.4823050/94‑1 (MQ=60)
                                                                    GGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTG                         >  SRR4302540.8737356/1‑94 (MQ=60)
                                                                    GGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTG                         >  SRR4302540.2978376/1‑94 (MQ=60)
                                                                    GGTCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTG                         >  SRR4302540.1813710/1‑94 (MQ=60)
                                                                      TCGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGAT                       <  SRR4302540.9861465/94‑1 (MQ=60)
                                                                       CGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATC                      <  SRR4302540.7336629/94‑1 (MQ=60)
                                                                       CGTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGGATTGCCTGGGGATTGATTAACGAAAATCTCGTTGCTC                      >  SRR4302540.533483/1‑94 (MQ=60)
                                                                        GTGAAGACGAATGGCTCCCCTTTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTTATTAATGTATATCTCGTTGATCA                     >  SRR4302540.9098961/1‑94 (MQ=60)
                                                                        GTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCA                     >  SRR4302540.10151750/1‑94 (MQ=60)
                                                                        GTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCA                     >  SRR4302540.7399012/1‑94 (MQ=60)
                                                                        GTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCA                     >  SRR4302540.2381213/1‑94 (MQ=60)
                                                                        GTGAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCA                     >  SRR4302540.8314861/1‑94 (MQ=60)
                                                                          GAAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAAC                   >  SRR4302540.4925108/1‑94 (MQ=60)
                                                                           AAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACC                  <  SRR4302540.3589827/94‑1 (MQ=60)
                                                                           AAGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACC                  >  SRR4302540.7247648/1‑94 (MQ=60)
                                                                            AGACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCT                 >  SRR4302540.6135701/1‑94 (MQ=60)
                                                                             GACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTT                >  SRR4302540.9618199/1‑94 (MQ=60)
                                                                             AACGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTT                <  SRR4302540.3112378/94‑1 (MQ=60)
                                                                               CGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTT              <  SRR4302540.6305237/94‑1 (MQ=60)
                                                                               CGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTT              <  SRR4302540.1197394/94‑1 (MQ=60)
                                                                               CGAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTT              >  SRR4302540.6951007/1‑94 (MQ=60)
                                                                                GAATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGGATTGATTAACGAAAATCTCGTTGATCAACCTTTTC             >  SRR4302540.824966/1‑94 (MQ=60)
                                                                                 AATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCT            >  SRR4302540.4136081/1‑94 (MQ=60)
                                                                                 AATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCT            <  SRR4302540.10068335/94‑1 (MQ=60)
                                                                                  ATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTC           >  SRR4302540.28669/1‑94 (MQ=60)
                                                                                  ATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTC           >  SRR4302540.4721257/1‑94 (MQ=60)
                                                                                  ATGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGCATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTC           >  SRR4302540.5921276/1‑94 (MQ=60)
                                                                                   TGGCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCG          <  SRR4302540.4853806/94‑1 (MQ=60)
                                                                                     GCTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGAT        <  SRR4302540.153385/94‑1 (MQ=60)
                                                                                      CTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATA       <  SRR4302540.9717944/94‑1 (MQ=60)
                                                                                      CTCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATA       >  SRR4302540.5982586/1‑94 (MQ=60)
                                                                                       TCCCCATTCGCCCGGGCACCGATGCCGCTCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAA      <  SRR4302540.947205/94‑1 (MQ=60)
                                                                                       TCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAA      >  SRR4302540.4864106/1‑94 (MQ=60)
                                                                                       TCCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAA      <  SRR4302540.5039813/94‑1 (MQ=60)
                                                                                        CCCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAA     <  SRR4302540.8554695/94‑1 (MQ=60)
                                                                                         CCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAAT    >  SRR4302540.378911/1‑94 (MQ=60)
                                                                                         CCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAAT    <  SRR4302540.304277/94‑1 (MQ=60)
                                                                                         CCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAAT    <  SRR4302540.7904294/94‑1 (MQ=60)
                                                                                         CCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAAT    <  SRR4302540.7904284/94‑1 (MQ=60)
                                                                                         CCCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAAT    <  SRR4302540.5645439/94‑1 (MQ=60)
                                                                                          CCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATA   <  SRR4302540.3691297/94‑1 (MQ=60)
                                                                                          CCATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATA   <  SRR4302540.3115300/94‑1 (MQ=60)
                                                                                           CATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATAC  >  SRR4302540.8366826/1‑94 (MQ=60)
                                                                                           CATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATAC  >  SRR4302540.8366825/1‑94 (MQ=60)
                                                                                           CATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATAC  <  SRR4302540.8097736/94‑1 (MQ=60)
                                                                                           CATTCGCCCGGGCACCGATGCCGCGCTGGTTGCAGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATAC  <  SRR4302540.9840916/94‑1 (MQ=60)
                                                                                           |                                                                                             
AAAAAGCGCGCGAGAAATCGAACGCCAAAATGATTGTTATCGATCCGCGATATACCGATACGGCTGCCGGTCGTGAAGACGAATGGCTCCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTATTGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATAC  >  NC_000913/1656832‑1657016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑