Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,601	T→C	A27A (GCT→GCC)	b1591 →	putative oxidoreductase component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,601	0	T	C	99.5%	597.4 / ‑4.8	187	A27A (GCT→GCC)	b1591	putative oxidoreductase component
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (80/106); total (81/106)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 194 total aligned reads displayed.

ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690

acaTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGcccc                                                                                         <  2:1850688/93‑1 (MQ=255)

 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  1:1313573/93‑1 (MQ=255)

 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        >  2:2735609/1‑93 (MQ=255)

 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        >  1:7894644/1‑93 (MQ=255)

 caTTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCa                                                                                        <  1:3412040/93‑1 (MQ=255)

     tCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCagaga                                                                                    <  1:5203233/93‑1 (MQ=255)

       aGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGt                                                                                  >  2:7681213/1‑93 (MQ=255)

        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 <  1:9129002/93‑1 (MQ=255)

        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 >  1:8885845/1‑93 (MQ=255)

        ggATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTg                                                                                 <  1:5509270/93‑1 (MQ=255)

         gATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGc                                                                                >  2:9588757/1‑93 (MQ=255)

          aTGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCg                                                                               <  1:5806557/93‑1 (MQ=255)

          aTGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCg                                                                               >  2:5367121/1‑93 (MQ=255)

           tGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCgg                                                                              >  1:6126780/1‑93 (MQ=255)

            gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGa                                                                             <  1:6648122/93‑1 (MQ=255)

            gTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGa                                                                             >  1:9001692/1‑93 (MQ=255)

             ttAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGaa                                                                            <  2:4727351/93‑1 (MQ=255)

               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          <  1:3232608/93‑1 (MQ=255)

               aGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGc                                                                          <  2:3853074/93‑1 (MQ=255)

                ggAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGcc                                                                         <  2:6807819/93‑1 (MQ=255)

                 gAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCg                                                                        >  1:8748728/1‑93 (MQ=255)

                 gAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCg                                                                        >  2:8563006/1‑93 (MQ=255)

                  aaTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGc                                                                       <  1:3071807/93‑1 (MQ=255)

                   aTGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCa                                                                      >  2:7843188/1‑93 (MQ=255)

                    tGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAc                                                                     <  1:4261462/93‑1 (MQ=255)

                     gACCCATTTTTCACAGCACGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAcc                                                                    >  2:5402769/1‑93 (MQ=255)

                       cccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctc                                                                  <  1:7259994/93‑1 (MQ=255)

                        ccATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACctct                                                                 <  1:4050864/93‑1 (MQ=255)

                          aTTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTgg                                                               >  2:8094364/1‑93 (MQ=255)

                          aTTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTgg                                                               >  1:3309283/1‑93 (MQ=255)

                           tttttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGt                                                              >  1:3361672/1‑93 (MQ=255)

                             tttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTg                                                            <  2:2593639/93‑1 (MQ=255)

                             tttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTg                                                            <  2:2123260/93‑1 (MQ=255)

                              ttCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGc                                                           <  2:7529221/93‑1 (MQ=255)

                               tCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCg                                                          >  2:3292568/1‑93 (MQ=255)

                               tCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCg                                                          >  2:5998266/1‑93 (MQ=255)

                                cacaGCAAGATAATGTTTCGGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTTCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGTTTGCgg                                                         <  1:2382495/93‑1 (MQ=255)

                                 acaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGt                                                        <  1:8518147/93‑1 (MQ=255)

                                 acaGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGGTGCGGt                                                        >  2:3769504/1‑93 (MQ=255)

                                       aGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTCCTCAc                                                  >  1:568670/1‑93 (MQ=255)

                                        gATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAcc                                                 >  1:9143443/1‑93 (MQ=255)

                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGGACTCACCa                                                >  2:3448296/1‑93 (MQ=255)

                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:3288415/93‑1 (MQ=255)

                                         aTAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCa                                                <  2:780705/93‑1 (MQ=255)

                                          tAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAg                                               <  2:8506243/93‑1 (MQ=255)

                                            aTTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTg                                             <  1:8383546/93‑1 (MQ=255)

                                              ttttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAc                                           >  1:1874355/1‑93 (MQ=255)

                                              ttttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAc                                           >  1:3491010/1‑93 (MQ=255)

                                               tttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACg                                          <  2:3931730/93‑1 (MQ=255)

                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         >  1:4911387/1‑93 (MQ=255)

                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         >  1:7533834/1‑93 (MQ=255)

                                                ttCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACgg                                         <  1:6864378/93‑1 (MQ=255)

                                                 tCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTCCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  1:3592783/93‑1 (MQ=255)

                                                 tCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  1:1157023/93‑1 (MQ=255)

                                                 tCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGc                                        <  1:7093726/93‑1 (MQ=255)

                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGGAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      >  2:9055966/1‑93 (MQ=255)

                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      >  2:8702982/1‑93 (MQ=255)

                                                   tGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTg                                      <  2:4151265/93‑1 (MQ=255)

                                                   tGTCGCGGCGCGCGTGGTGGGCGCGCTGTTTTATTACGCCCCAGATAGTGCGGAAGCCGCACCTCTGGGTGCGGTACTCATCAGTGTCGGCTg                                      >  2:8962388/1‑93 (MQ=255)

                                                    gTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAGGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTgg                                     <  1:6443634/93‑1 (MQ=255)

                                                    gTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTgg                                     >  2:4553341/1‑93 (MQ=255)

                                                     tCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTggg                                    <  1:1610062/93‑1 (MQ=255)

                                                      cgcgGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGa                                   <  1:2325896/93‑1 (MQ=255)

                                                       gcggcgCGCGTGTTGGGCGCCCTGTTTTATTACGCCCCAGAGAGTGCGGAAACCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGaa                                  <  1:5919883/93‑1 (MQ=255)

                                                          gcgcgcGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGAGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACt                               >  1:2077254/1‑93 (MQ=255)

                                                           cgcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTc                              <  1:3157531/93‑1 (MQ=255)

                                                           cgcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTc                              >  2:7370097/1‑93 (MQ=255)

                                                           cgcgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTc                              <  2:9272417/93‑1 (MQ=255)

                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            >  1:1997724/1‑93 (MQ=255)

                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            <  1:8183357/93‑1 (MQ=255)

                                                             cgcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAg                            <  1:6948381/93‑1 (MQ=255)

                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:6391116/93‑1 (MQ=255)

                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:9347607/93‑1 (MQ=255)

                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  1:6778499/93‑1 (MQ=255)

                                                              gcgTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGt                           <  2:9447180/93‑1 (MQ=255)

                                                                 tgtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGACACTCAGTTGc                        >  2:9301165/1‑93 (MQ=255)

                                                                  gtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGcc                       <  1:5025163/93‑1 (MQ=255)

                                                                  gtTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGcc                       >  1:6979606/1‑93 (MQ=255)

                                                                   ttGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCct                      >  2:8664004/1‑93 (MQ=255)

                                                                   ttGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCct                      >  1:3436196/1‑93 (MQ=255)

                                                                      ggCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTa                   <  1:2265188/93‑1 (MQ=255)

                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCCGTCGCCTCTACCa                >  2:138133/1‑93 (MQ=255)

                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                >  2:9882174/1‑93 (MQ=255)

                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                <  1:4125826/93‑1 (MQ=255)

                                                                         gcgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCa                <  2:1660516/93‑1 (MQ=255)

                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               <  2:3354477/93‑1 (MQ=255)

                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               <  2:2203276/93‑1 (MQ=255)

                                                                          cgcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAg               >  2:1599621/1‑93 (MQ=255)

                                                                           gcTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGa              <  2:2992899/93‑1 (MQ=255)

                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          <  2:8885844/93‑1 (MQ=255)

                                                                               ttttATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCg          <  2:5185013/93‑1 (MQ=255)

                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  1:9603298/93‑1 (MQ=255)

                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  1:4365240/93‑1 (MQ=255)

                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  2:9848520/93‑1 (MQ=255)

                                                                                  tattaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCa       <  2:624545/93‑1 (MQ=255)

                                                                                   attaCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAt      <  2:1229691/93‑1 (MQ=255)

                                                                                     taCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTa    <  1:2171217/93‑1 (MQ=255)

                                                                                     taCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTa    >  1:531731/1‑93 (MQ=255)

                                                                                      aCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAg   <  1:2570291/93‑1 (MQ=255)

                                                                                       cGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAgc  <  2:6437188/93‑1 (MQ=255)

ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC  >  NC_000913/1662511‑1662690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCC  >  NC_000913/1662508‑1662693

CGCACATTTCAGGATGTTTGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCC                                                                                              <  SRR4302540.6090712/94‑1 (MQ=60)

CGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCC                                                                                              <  SRR4302540.10073863/94‑1 (MQ=60)

 GCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCTAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCC                                                                                             >  SRR4302540.6137073/1‑94 (MQ=60)

 GCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCC                                                                                             >  SRR4302540.6137072/1‑94 (MQ=60)

 GCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCC                                                                                             >  SRR4302540.3054966/1‑94 (MQ=60)

   ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCA                                                                                           <  SRR4302540.1589529/94‑1 (MQ=60)

   ACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCA                                                                                           >  SRR4302540.7986764/1‑94 (MQ=60)

    CATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAG                                                                                          <  SRR4302540.1327226/94‑1 (MQ=60)

    CATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAG                                                                                          <  SRR4302540.3449198/94‑1 (MQ=60)

      TTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAATATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAG                                                                                        <  SRR4302540.7405315/94‑1 (MQ=60)

      TTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAG                                                                                        <  SRR4302540.9357684/94‑1 (MQ=60)

       TTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGA                                                                                       <  SRR4302540.5037223/94‑1 (MQ=60)

        TCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAG                                                                                      <  SRR4302540.5259910/94‑1 (MQ=60)

        TCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAG                                                                                      <  SRR4302540.7844736/94‑1 (MQ=60)

        TCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAG                                                                                      >  SRR4302540.2563720/1‑94 (MQ=60)

         CAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGT                                                                                     >  SRR4302540.6538870/1‑94 (MQ=60)

         CAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGT                                                                                     <  SRR4302540.4127910/94‑1 (MQ=60)

          AGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTG                                                                                    >  SRR4302540.8988701/1‑94 (MQ=60)

           GGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGC                                                                                   <  SRR4302540.5569945/94‑1 (MQ=60)

           GGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGC                                                                                   <  SRR4302540.9238726/94‑1 (MQ=60)

            GATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGTG                                                                                  <  SRR4302540.8118323/94‑1 (MQ=60)

            GATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCG                                                                                  <  SRR4302540.5393980/94‑1 (MQ=60)

            GATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCG                                                                                  <  SRR4302540.6774270/94‑1 (MQ=60)

             ATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGG                                                                                 <  SRR4302540.5870719/94‑1 (MQ=60)

             ATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGG                                                                                 >  SRR4302540.6195019/1‑94 (MQ=60)

              TGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGA                                                                                >  SRR4302540.9109690/1‑94 (MQ=60)

               GTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCTCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAA                                                                               <  SRR4302540.1935943/94‑1 (MQ=60)

               GTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAA                                                                               <  SRR4302540.6721665/94‑1 (MQ=60)

                TTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAG                                                                              <  SRR4302540.3251973/94‑1 (MQ=60)

                TTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAG                                                                              <  SRR4302540.2920855/94‑1 (MQ=60)

                  AGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCC                                                                            <  SRR4302540.3267759/94‑1 (MQ=60)

                   GGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCG                                                                           >  SRR4302540.8850250/1‑94 (MQ=60)

                     AATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCA                                                                         <  SRR4302540.3105511/94‑1 (MQ=60)

                      ATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCAC                                                                        <  SRR4302540.6937898/94‑1 (MQ=60)

                       TGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACC                                                                       <  SRR4302540.4307816/94‑1 (MQ=60)

                          CCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCT                                                                    <  SRR4302540.7341006/94‑1 (MQ=60)

                           CCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTG                                                                   <  SRR4302540.3983293/94‑1 (MQ=60)

                           CCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTG                                                                   <  SRR4302540.4094538/94‑1 (MQ=60)

                           CCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTG                                                                   <  SRR4302540.6256236/94‑1 (MQ=60)

                            CATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGG                                                                  >  SRR4302540.3345538/1‑94 (MQ=60)

                             ATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGT                                                                 >  SRR4302540.3398470/1‑94 (MQ=60)

                                   CACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGT                                                           >  SRR4302540.339915/1‑94 (MQ=60)

                                   CACAGCAAGATAATGTTTCGGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTTCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGTTTGCGGT                                                           <  SRR4302540.2408166/94‑1 (MQ=60)

                                    ACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTA                                                          <  SRR4302540.8617137/94‑1 (MQ=60)

                                     aagcaagagacgtgtgcattgGCGGCGCGCGTGTTGGGCGCACTGTTGTATTGCGCCCCAGAAAGTGCGGAGGCCGCACCTCTGGTTGCGGTAC                                                         <  SRR4302540.6536140/73‑1 (MQ=50)

                                     CAGCACGATAATTTTTCTGTCGCGGCGCGCGTGTTGGTCTCGCTGTTTTATTACGCCCCAGAGAGTTCGGAAGCCGCACCTCTGGTTGCGGTAC                                                         <  SRR4302540.6536139/94‑1 (MQ=60)

                                        GAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCA                                                      <  SRR4302540.2256895/94‑1 (MQ=60)

                                        CAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCA                                                      >  SRR4302540.3131576/1‑94 (MQ=60)

                                         AAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTCCTCAC                                                     >  SRR4302540.574848/1‑94 (MQ=60)

                                         AAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCAC                                                     >  SRR4302540.574849/1‑94 (MQ=60)

                                          AGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACC                                                    >  SRR4302540.9253281/1‑94 (MQ=60)

                                          AGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACC                                                    <  SRR4302540.4338736/94‑1 (MQ=60)

                                          AGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACC                                                    <  SRR4302540.5311825/94‑1 (MQ=60)

                                              AATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTG                                                <  SRR4302540.9625183/94‑1 (MQ=60)

                                               ATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGA                                               <  SRR4302540.897641/94‑1 (MQ=60)

                                               ATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGA                                               <  SRR4302540.8480816/94‑1 (MQ=60)

                                                TTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAC                                              >  SRR4302540.1894885/1‑94 (MQ=60)

                                                TTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGAC                                              >  SRR4302540.3529030/1‑94 (MQ=60)

                                                 TTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGCAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACG                                             <  SRR4302540.1803484/94‑1 (MQ=60)

                                                  TTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGG                                            >  SRR4302540.4964273/1‑94 (MQ=60)

                                                  TTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGG                                            >  SRR4302540.7618203/1‑94 (MQ=60)

                                                   TTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGC                                           <  SRR4302540.6940452/94‑1 (MQ=60)

                                                    TCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTCCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCT                                          <  SRR4302540.3632030/94‑1 (MQ=60)

                                                    TCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCT                                          <  SRR4302540.7172667/94‑1 (MQ=60)

                                                    TCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCT                                          <  SRR4302540.1169059/94‑1 (MQ=60)

                                                       GTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAGGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGG                                       <  SRR4302540.6515360/94‑1 (MQ=60)

                                                        TCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGA                                      <  SRR4302540.1627700/94‑1 (MQ=60)

                                                         CGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAA                                     <  SRR4302540.2350943/94‑1 (MQ=60)

                                                          GCGGCGCGCGTGTTGGGCGCCCTGTTTTATTACGCCCCAGAGAGTGCGGAAACCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAA                                    <  SRR4302540.5985282/94‑1 (MQ=60)

                                                            GGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGAGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACT                                  >  SRR4302540.2099575/1‑94 (MQ=60)

                                                              CGCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCA                                <  SRR4302540.3191895/94‑1 (MQ=60)

                                                               GCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAG                               >  SRR4302540.2019366/1‑94 (MQ=60)

                                                               GCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAG                               >  SRR4302540.5256941/1‑94 (MQ=60)

                                                               CCGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAG                               <  SRR4302540.3293475/94‑1 (MQ=60)

                                                                CGCGTGTTGGTCGCGCTGTTTTATTACGACCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAAT                              <  SRR4302540.9060867/94‑1 (MQ=60)

                                                                CGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGT                              <  SRR4302540.3356502/94‑1 (MQ=60)

                                                                CGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGT                              <  SRR4302540.8278411/94‑1 (MQ=60)

                                                                CGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGT                              <  SRR4302540.3527130/94‑1 (MQ=60)

                                                                CGCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGT                              <  SRR4302540.7025702/94‑1 (MQ=60)

                                                                 GCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTG                             <  SRR4302540.9458955/94‑1 (MQ=60)

                                                                 GCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTG                             >  SRR4302540.2328263/1‑94 (MQ=60)

                                                                 GCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTG                             <  SRR4302540.6853427/94‑1 (MQ=60)

                                                                 GCGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTG                             <  SRR4302540.6462260/94‑1 (MQ=60)

                                                                  CGTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGG                            <  SRR4302540.4674706/94‑1 (MQ=60)

                                                                   GTGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGC                           >  SRR4302540.6134294/1‑94 (MQ=60)

                                                                    TGTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCC                          >  SRR4302540.7057142/1‑94 (MQ=60)

                                                                     GTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCT                         >  SRR4302540.3473549/1‑94 (MQ=60)

                                                                     GTTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCT                         <  SRR4302540.5079557/94‑1 (MQ=60)

                                                                      TTGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTC                        >  SRR4302540.7150539/1‑94 (MQ=60)

                                                                      GTGGGCGCGCTGTTTTATTCCGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTGCTCACCAGTGACGGCTGGGAAACTCAGTGGCCTC                        <  SRR4302540.7226102/94‑1 (MQ=60)

                                                                       TGGGCGCGCTTTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCT                       <  SRR4302540.2113726/94‑1 (MQ=60)

                                                                       TGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCT                       >  SRR4302540.4243797/1‑94 (MQ=60)

                                                                       TGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCT                       >  SRR4302540.9137166/1‑94 (MQ=60)

                                                                       TGGGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCT                       <  SRR4302540.8494011/94‑1 (MQ=60)

                                                                         GGCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTAC                     <  SRR4302540.2289498/94‑1 (MQ=60)

                                                                          GCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACC                    >  SRR4302540.7036484/1‑94 (MQ=60)

                                                                          GCGCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACC                    >  SRR4302540.8478544/1‑94 (MQ=60)

                                                                            GCGCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAG                  <  SRR4302540.4170399/94‑1 (MQ=60)

                                                                              GCTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAA                >  SRR4302540.6943915/1‑94 (MQ=60)

                                                                               CTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAG               <  SRR4302540.2033326/94‑1 (MQ=60)

                                                                               CTGTTTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAG               >  SRR4302540.5587126/1‑94 (MQ=60)

                                                                                   TTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTC           >  SRR4302540.8797145/1‑94 (MQ=60)

                                                                                   TTTATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTC           >  SRR4302540.2401060/1‑94 (MQ=60)

                                                                                     TATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAT         <  SRR4302540.4412683/94‑1 (MQ=60)

                                                                                     TATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAT         <  SRR4302540.5940720/94‑1 (MQ=60)

                                                                                     TATTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCAT         <  SRR4302540.9716571/94‑1 (MQ=60)

                                                                                       TTACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTA       >  SRR4302540.537480/1‑94 (MQ=60)

                                                                                        TACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAG      <  SRR4302540.2194631/94‑1 (MQ=60)

                                                                                         ACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC     <  SRR4302540.2598005/94‑1 (MQ=60)

                                                                                         ACGCCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGC     <  SRR4302540.3532104/94‑1 (MQ=60)

                                                                                            CCCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCC  <  SRR4302540.9581621/94‑1 (MQ=60)

CGCACATTTCAGGATGTTAGGAATGACCCATTTTTCACAGCAAGATAATTTTTCTGTCGCGGCGCGCGTGTTGGGCGCGCTGTTTTATTACGCTCCAGAGAGTGCGGAAGCCGCACCTCTGGTTGCGGTACTCACCAGTGACGGCTGGGAAACTCAGTGGCCTCTACCAGAAGCGTCATTAGCGCC  >  NC_000913/1662508‑1662693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`