Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,587,936 A→G R30R (CGT→CGC)  b1505 ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,587,9360AG100.0% 724.7 / NA 223R30R (CGT→CGC) b1505putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (102/121);  total (102/121)

Only 100 of 229 total aligned reads displayed.

GACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCACGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCAT  >  NC_000913/1587847‑1588025
                                                                                         |                                                                                         
gACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCgcgc                                                                                        >  2:795583/1‑93 (MQ=255)
gACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCgcgc                                                                                        >  1:473217/1‑93 (MQ=255)
  cGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCtt                                                                                      >  1:6114851/1‑93 (MQ=255)
   gtgtTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTa                                                                                     <  2:1961966/93‑1 (MQ=255)
    tgtTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTAc                                                                                    <  2:2068851/93‑1 (MQ=255)
    tgtTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGATCCTGACCGCGCTTAc                                                                                    >  2:6298915/1‑93 (MQ=255)
     gtTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACt                                                                                   <  2:2770183/93‑1 (MQ=255)
     gtTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACt                                                                                   <  1:2721287/93‑1 (MQ=255)
       tCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGt                                                                                 <  1:1872885/93‑1 (MQ=255)
       tCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGt                                                                                 >  1:219495/1‑93 (MQ=255)
        ccAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTa                                                                                >  2:5291699/1‑93 (MQ=255)
        ccAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTa                                                                                <  2:5102452/93‑1 (MQ=255)
        ccAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTa                                                                                <  2:3918899/93‑1 (MQ=255)
         cAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAg                                                                               <  2:1218272/93‑1 (MQ=255)
          aGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGa                                                                              <  1:39313/93‑1 (MQ=255)
          aGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGa                                                                              >  1:4318521/1‑93 (MQ=255)
          aGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGa                                                                              <  1:1152954/93‑1 (MQ=255)
            tAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAAc                                                                            >  1:6301236/1‑93 (MQ=255)
            tAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAAc                                                                            <  2:1816494/93‑1 (MQ=255)
             aaCTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACa                                                                           >  1:5680944/1‑93 (MQ=255)
             aaCTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACa                                                                           <  2:422587/93‑1 (MQ=255)
               cTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGa                                                                         <  1:617544/93‑1 (MQ=255)
               cTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGa                                                                         >  1:6190372/1‑93 (MQ=255)
                tttGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGAt                                                                        <  2:5453754/93‑1 (MQ=255)
                   ggCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTaa                                                                     <  1:4263145/93‑1 (MQ=255)
                     cGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTaaaa                                                                   <  1:247791/93‑1 (MQ=255)
                     cGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTaaaa                                                                   <  2:2366025/93‑1 (MQ=255)
                      gACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaat                                                                  <  1:1517359/93‑1 (MQ=255)
                       aCTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaata                                                                 <  2:33346/93‑1 (MQ=255)
                       aCTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaata                                                                 >  1:4451257/1‑93 (MQ=255)
                        cTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaataa                                                                <  1:808198/93‑1 (MQ=255)
                         tGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaataat                                                               <  1:6095222/93‑1 (MQ=255)
                         tGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaataat                                                               >  1:6027763/1‑93 (MQ=255)
                         tGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAaataat                                                               >  2:2879101/1‑93 (MQ=255)
                          ggCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATc                                                              <  1:2562058/93‑1 (MQ=255)
                           gCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCa                                                             >  1:4768375/1‑93 (MQ=255)
                            cACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCaa                                                            >  1:729637/1‑93 (MQ=255)
                             aCTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAAt                                                           <  1:4002190/93‑1 (MQ=255)
                             aCTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGAAGAACAGATTAAAATAATCaaa                                                           >  1:1933184/1‑92 (MQ=255)
                              cTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATa                                                          >  2:3218795/1‑93 (MQ=255)
                               tGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATaa                                                         <  1:3953613/93‑1 (MQ=255)
                                 aaaaaTGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAAt                                                       <  2:2381031/93‑1 (MQ=255)
                                 aaaaaTGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAAt                                                       <  2:1481442/93‑1 (MQ=255)
                                  aaaaTGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAAtt                                                      >  1:6009695/1‑93 (MQ=255)
                                    aaTGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGCACAGATTAAAATAATCAATAAATtgt                                                    >  1:3505709/1‑93 (MQ=255)
                                    aaTGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATtgt                                                    <  2:5229414/93‑1 (MQ=255)
                                      tGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTgtgt                                                  >  2:687966/1‑93 (MQ=255)
                                       gTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTc                                                 <  1:4191096/93‑1 (MQ=255)
                                        tCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCt                                                <  1:5786471/93‑1 (MQ=255)
                                        tCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCt                                                <  1:5103109/93‑1 (MQ=255)
                                        tCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCt                                                >  2:2269559/1‑93 (MQ=255)
                                           tACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCt                                             >  2:4462165/1‑93 (MQ=255)
                                             ccGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCa                                           <  1:1349884/93‑1 (MQ=255)
                                              cGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCAt                                          <  1:3556337/93‑1 (MQ=255)
                                              cGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCAt                                          >  2:3526382/1‑93 (MQ=255)
                                                tAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTg                                        <  2:5254558/93‑1 (MQ=255)
                                                tAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTg                                        <  1:4840719/93‑1 (MQ=255)
                                                 aaTTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTgg                                       <  2:3760107/93‑1 (MQ=255)
                                                 aaTTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTgg                                       <  1:36540/93‑1 (MQ=255)
                                                 aaTTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTgg                                       <  2:827995/93‑1 (MQ=255)
                                                   tttCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGgtg                                     <  2:5668597/93‑1 (MQ=255)
                                                   tttCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGgtg                                     <  2:4368225/93‑1 (MQ=255)
                                                   tttCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGgtg                                     >  1:5359396/1‑93 (MQ=255)
                                                    ttCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTAACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGgtgt                                    >  1:3390683/1‑93 (MQ=255)
                                                     tCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGtc                                   <  1:3343183/93‑1 (MQ=255)
                                                       cAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGtctc                                 <  2:1059951/93‑1 (MQ=255)
                                                       cAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGtctc                                 <  2:6129155/93‑1 (MQ=255)
                                                       cAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGtctc                                 >  1:5515236/1‑93 (MQ=255)
                                                        aaGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTcc                                <  1:107120/93‑1 (MQ=255)
                                                        aaGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTcc                                <  2:6095248/93‑1 (MQ=255)
                                                         aGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCg                               >  1:1737780/1‑93 (MQ=255)
                                                         aGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCg                               <  1:3808105/93‑1 (MQ=255)
                                                          gctgctGAGAGATGCTTATTTGTTCATGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCgg                              <  1:3744853/93‑1 (MQ=255)
                                                           ctgctgAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGt                             >  1:2871004/1‑93 (MQ=255)
                                                            tgctgAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTa                            >  1:2306573/1‑93 (MQ=255)
                                                             gctgAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTag                           <  1:1230345/93‑1 (MQ=255)
                                                              ctgAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTagg                          <  2:6048754/93‑1 (MQ=255)
                                                               tgAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTagga                         >  1:1612541/1‑93 (MQ=255)
                                                                gagagaTGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTaggag                        >  2:6196109/1‑93 (MQ=255)
                                                                  gagaTGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGc                      <  2:4742373/93‑1 (MQ=255)
                                                                   agaTGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCt                     <  1:2016204/93‑1 (MQ=255)
                                                                    gaTGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCtt                    >  2:5891289/1‑93 (MQ=255)
                                                                     aTGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTg                   >  1:3305570/1‑93 (MQ=255)
                                                                     aTGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTg                   >  2:5832407/1‑93 (MQ=255)
                                                                      tGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGa                  >  2:3644419/1‑93 (MQ=255)
                                                                      tGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGa                  >  1:5193902/1‑93 (MQ=255)
                                                                      tGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGa                  >  2:4077107/1‑93 (MQ=255)
                                                                       gCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGAc                 <  1:3844875/93‑1 (MQ=255)
                                                                         ttATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACgg               >  2:1927783/1‑93 (MQ=255)
                                                                         ttATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACgg               >  2:4863599/1‑93 (MQ=255)
                                                                         ttATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACgg               >  2:2731559/1‑93 (MQ=255)
                                                                           aTTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGgtg             <  1:2394833/93‑1 (MQ=255)
                                                                            tttGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGgtgt            <  2:3130199/93‑1 (MQ=255)
                                                                             ttGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTa           >  2:5583486/1‑93 (MQ=255)
                                                                               gTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAAc         <  1:1701181/93‑1 (MQ=255)
                                                                                 tCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCa       >  2:3009447/1‑93 (MQ=255)
                                                                                  ccTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCAc      >  1:5904850/1‑93 (MQ=255)
                                                                                    tGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTc    <  2:1677931/93‑1 (MQ=255)
                                                                                    tGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTc    >  1:4377156/1‑93 (MQ=255)
                                                                                     gACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCa   >  2:1644944/1‑93 (MQ=255)
                                                                                         |                                                                                         
GACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCACGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCAT  >  NC_000913/1587847‑1588025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTGACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCACGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATTCGA  >  NC_000913/1587843‑1588029
                                                                                             |                                                                                             
GCGTGACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCG                                                                                               <  SRR4302533.1065704/94‑1 (MQ=60)
  GTGACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCG                                                                                             <  SRR4302533.4043958/94‑1 (MQ=60)
   TGACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGC                                                                                            >  SRR4302533.476046/1‑94 (MQ=60)
   TGACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGC                                                                                            >  SRR4302533.5599794/1‑94 (MQ=60)
     ACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTT                                                                                          <  SRR4302533.4992176/94‑1 (MQ=60)
     ACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTT                                                                                          >  SRR4302533.6151746/1‑94 (MQ=60)
       GTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTAC                                                                                        <  SRR4302533.6101492/94‑1 (MQ=60)
        TGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACT                                                                                       <  SRR4302533.3816359/94‑1 (MQ=60)
         GTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTG                                                                                      <  SRR4302533.2737989/94‑1 (MQ=60)
          TTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGT                                                                                     >  SRR4302533.220869/1‑94 (MQ=60)
           TCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTA                                                                                    <  SRR4302533.1884302/94‑1 (MQ=60)
            CCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAG                                                                                   <  SRR4302533.379463/94‑1 (MQ=60)
            CCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAG                                                                                   >  SRR4302533.2842229/1‑94 (MQ=60)
             CAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGA                                                                                  <  SRR4302533.4322846/94‑1 (MQ=60)
             CAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGA                                                                                  >  SRR4302533.4344530/1‑94 (MQ=60)
             CAGTAACTTTGGCGACTGGCACTAAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGA                                                                                  <  SRR4302533.5158034/94‑1 (MQ=60)
              AGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAA                                                                                 <  SRR4302533.2470768/94‑1 (MQ=60)
              AGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAA                                                                                 <  SRR4302533.39574/94‑1 (MQ=60)
              AGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAA                                                                                 <  SRR4302533.1159857/94‑1 (MQ=60)
               GTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAAC                                                                                >  SRR4302533.6338872/1‑94 (MQ=60)
                TAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACA                                                                               >  SRR4302533.5715257/1‑94 (MQ=60)
                 AACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTGCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAG                                                                              <  SRR4302533.3247394/94‑1 (MQ=60)
                  ACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGA                                                                             <  SRR4302533.4375858/94‑1 (MQ=60)
                  ACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGA                                                                             >  SRR4302533.6227519/1‑94 (MQ=60)
                   CTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGAT                                                                            <  SRR4302533.621164/94‑1 (MQ=60)
                       GGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAA                                                                        <  SRR4302533.4288827/94‑1 (MQ=60)
                       GGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAA                                                                        <  SRR4302533.6186463/94‑1 (MQ=60)
                        GCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAA                                                                       <  SRR4302533.5227985/94‑1 (MQ=60)
                         CGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAAT                                                                      <  SRR4302533.3997722/94‑1 (MQ=60)
                         CGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAAT                                                                      <  SRR4302533.249322/94‑1 (MQ=60)
                          GACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATA                                                                     >  SRR4302533.4478170/1‑94 (MQ=60)
                          GACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATA                                                                     <  SRR4302533.1526493/94‑1 (MQ=60)
                           ACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAA                                                                    >  SRR4302533.2028941/1‑94 (MQ=60)
                           ACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAA                                                                    >  SRR4302533.6043008/1‑94 (MQ=60)
                            CTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAAT                                                                   <  SRR4302533.812948/94‑1 (MQ=60)
                            CTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAAT                                                                   >  SRR4302533.3566538/1‑94 (MQ=60)
                            CTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAAT                                                                   >  SRR4302533.6227101/1‑94 (MQ=60)
                            CTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAAT                                                                   >  SRR4302533.6064290/1‑94 (MQ=60)
                             TGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATC                                                                  <  SRR4302533.6132045/94‑1 (MQ=60)
                              GGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCA                                                                 <  SRR4302533.2577831/94‑1 (MQ=60)
                              GGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCA                                                                 <  SRR4302533.384026/94‑1 (MQ=60)
                              GGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCA                                                                 <  SRR4302533.4446454/94‑1 (MQ=60)
                              GGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCA                                                                 >  SRR4302533.4797278/1‑94 (MQ=60)
                              GGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCA                                                                 <  SRR4302533.552063/94‑1 (MQ=60)
                               GCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAA                                                                >  SRR4302533.733940/1‑94 (MQ=60)
                                CACTGAAAAATGTCGTACCGTAATTTTCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAAA                                                               >  SRR4302533.3667496/1‑94 (MQ=60)
                                CACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAAT                                                               <  SRR4302533.3961927/94‑1 (MQ=60)
                                CACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGAAGAACAGATTAAAATAATCAAA                                                               >  SRR4302533.1944982/1‑94 (MQ=60)
                                 ACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATA                                                              >  SRR4302533.5948837/1‑94 (MQ=60)
                                 ACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATA                                                              <  SRR4302533.4026561/94‑1 (MQ=60)
                                  CTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAA                                                             <  SRR4302533.1759458/94‑1 (MQ=60)
                                   TGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAA                                                            <  SRR4302533.3977718/94‑1 (MQ=60)
                                     AAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATT                                                          >  SRR4302533.6046105/1‑94 (MQ=60)
                                     AAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATT                                                          <  SRR4302533.2340635/94‑1 (MQ=60)
                                       AAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGCACAGATTAAAATAATCAATAAATTGT                                                        >  SRR4302533.3527319/1‑94 (MQ=60)
                                         ATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGT                                                      >  SRR4302533.4621825/1‑94 (MQ=60)
                                          TGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTC                                                     >  SRR4302533.794597/1‑94 (MQ=60)
                                           GTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCT                                                    <  SRR4302533.4769603/94‑1 (MQ=60)
                                           GTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCT                                                    <  SRR4302533.4216463/94‑1 (MQ=60)
                                            TCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTG                                                   <  SRR4302533.5133936/94‑1 (MQ=60)
                                            TCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTG                                                   <  SRR4302533.5821423/94‑1 (MQ=60)
                                             CGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGC                                                  >  SRR4302533.1741500/1‑94 (MQ=60)
                                             CGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGC                                                  >  SRR4302533.140865/1‑94 (MQ=60)
                                              GTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCT                                                 >  SRR4302533.4798367/1‑94 (MQ=60)
                                               TACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTC                                                >  SRR4302533.3761641/1‑94 (MQ=60)
                                                 CCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCAT                                              <  SRR4302533.1358003/94‑1 (MQ=60)
                                                  CGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATT                                             >  SRR4302533.4295123/1‑94 (MQ=60)
                                                  CGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATT                                             <  SRR4302533.3578255/94‑1 (MQ=60)
                                                  CGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATT                                             >  SRR4302533.13059/1‑94 (MQ=60)
                                                  CGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATT                                             >  SRR4302533.6261525/1‑94 (MQ=60)
                                                   GTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTATAATAATCAATAAATTGTGTCTGCTCATTG                                            >  SRR4302533.1649949/1‑94 (MQ=60)
                                                   GTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTG                                            >  SRR4302533.6133764/1‑94 (MQ=60)
                                                   CTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTG                                            <  SRR4302533.1541532/94‑1 (MQ=60)
                                                    TAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGG                                           >  SRR4302533.2198531/1‑94 (MQ=60)
                                                    TAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGG                                           >  SRR4302533.6057442/1‑94 (MQ=60)
                                                    TAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGG                                           <  SRR4302533.4870059/94‑1 (MQ=60)
                                                     AATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGT                                          <  SRR4302533.36780/94‑1 (MQ=60)
                                                      ATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTG                                         <  SRR4302533.5683289/94‑1 (MQ=60)
                                                      ATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTG                                         >  SRR4302533.3876835/1‑94 (MQ=60)
                                                      ATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTG                                         >  SRR4302533.5391579/1‑94 (MQ=60)
                                                       TTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGT                                        <  SRR4302533.3466301/94‑1 (MQ=60)
                                                       TTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTAACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGT                                        >  SRR4302533.3411623/1‑94 (MQ=60)
                                                        TTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTC                                       >  SRR4302533.4042552/1‑94 (MQ=60)
                                                         TCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCT                                      <  SRR4302533.3363843/94‑1 (MQ=60)
                                                         TCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCT                                      <  SRR4302533.1150424/94‑1 (MQ=60)
                                                          CCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTC                                     >  SRR4302533.2543441/1‑94 (MQ=60)
                                                          CCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTC                                     >  SRR4302533.5548532/1‑94 (MQ=60)
                                                            AAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCG                                   <  SRR4302533.107791/94‑1 (MQ=60)
                                                            AAGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCG                                   >  SRR4302533.1748322/1‑94 (MQ=60)
                                                             AGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGG                                  <  SRR4302533.3831401/94‑1 (MQ=60)
                                                             AGCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGG                                  <  SRR4302533.3468862/94‑1 (MQ=60)
                                                              GCTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGT                                 >  SRR4302533.2888670/1‑94 (MQ=60)
                                                              GCTGCTGAGAGATGCTTATTTGTTCATGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGT                                 <  SRR4302533.3767820/94‑1 (MQ=60)
                                                               CTGCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTA                                >  SRR4302533.2320863/1‑94 (MQ=60)
                                                                 GCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGG                              <  SRR4302533.1237712/94‑1 (MQ=60)
                                                                 GCTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGG                              >  SRR4302533.4231038/1‑94 (MQ=60)
                                                                  CTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGA                             >  SRR4302533.725934/1‑94 (MQ=60)
                                                                  CTGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGA                             >  SRR4302533.1622316/1‑94 (MQ=60)
                                                                   TGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAG                            >  SRR4302533.659630/1‑94 (MQ=60)
                                                                   TGAGAGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAG                            <  SRR4302533.3970650/94‑1 (MQ=60)
                                                                       AGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTT                        <  SRR4302533.1205036/94‑1 (MQ=60)
                                                                       AGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTT                        >  SRR4302533.512443/1‑94 (MQ=60)
                                                                       AGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTT                        <  SRR4302533.5834717/94‑1 (MQ=60)
                                                                       AGATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTT                        <  SRR4302533.2028626/94‑1 (MQ=60)
                                                                        GATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTG                       >  SRR4302533.3325980/1‑94 (MQ=60)
                                                                        GATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTG                       <  SRR4302533.1525346/94‑1 (MQ=60)
                                                                        GATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTG                       >  SRR4302533.2910084/1‑94 (MQ=60)
                                                                         ATGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGA                      >  SRR4302533.5225230/1‑94 (MQ=60)
                                                                          TGCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGAC                     <  SRR4302533.3347137/94‑1 (MQ=60)
                                                                           GCTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACG                    <  SRR4302533.3868356/94‑1 (MQ=60)
                                                                            CTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGG                   >  SRR4302533.1739893/1‑94 (MQ=60)
                                                                            CTTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGG                   >  SRR4302533.2303496/1‑94 (MQ=60)
                                                                             TTATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGT                  >  SRR4302533.4548643/1‑94 (MQ=60)
                                                                               ATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGT                <  SRR4302533.2487942/94‑1 (MQ=60)
                                                                               ATTTGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGT                <  SRR4302533.2409614/94‑1 (MQ=60)
                                                                                  TGTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAAC             >  SRR4302533.1972849/1‑94 (MQ=60)
                                                                                   GTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACC            <  SRR4302533.5785810/94‑1 (MQ=60)
                                                                                   GTTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACC            <  SRR4302533.1711492/94‑1 (MQ=60)
                                                                                    TTCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCA           >  SRR4302533.6172548/1‑94 (MQ=60)
                                                                                     TCCTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCAC          >  SRR4302533.5940512/1‑94 (MQ=60)
                                                                                       CTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTC        <  SRR4302533.1915426/94‑1 (MQ=60)
                                                                                       CTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTC        >  SRR4302533.4403581/1‑94 (MQ=60)
                                                                                       CTGACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATCGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTC        >  SRR4302533.3393334/1‑94 (MQ=60)
                                                                                         GACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCAT      >  SRR4302533.2697121/1‑94 (MQ=60)
                                                                                          ACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATT     >  SRR4302533.2153969/1‑94 (MQ=60)
                                                                                          ACCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGAAGGAGGCTTGACGGTGTAACCACTCATT     >  SRR4302533.6096570/1‑94 (MQ=60)
                                                                                           CCGCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATTC    <  SRR4302533.3359830/94‑1 (MQ=60)
                                                                                             GCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATTCGC  >  SRR4302533.1431780/1‑94 (MQ=60)
                                                                                             GCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATTCGA  <  SRR4302533.5375762/94‑1 (MQ=60)
                                                                                             GCGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATTCGA  >  SRR4302533.6009435/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCGTGACGTGTTCCAGTAACTTTGGCGACTGGCACTGAAAAATGTCGTACCGTAATTTCCAAGCTGCTGAGAGATGCTTATTTGTTCCTGACCACGCTTACTGTAGAACAGATTAAAATAATCAATAAATTGTGTCTGCTCATTGGTGTCTCCGGTAGGAGGCTTGACGGTGTAACCACTCATTCGA  >  NC_000913/1587843‑1588029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑