Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,599,102	T→C	H55R (CAT→CGT)	ydeW ←	putative transcriptional regulator, sorC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,599,102	0	T	C	99.6%	789.8 / NA	243	H55R (CAT→CGT)	ydeW	putative transcriptional regulator, sorC family
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (137/105); total (138/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

GCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAA  >  NC_000913/1599013‑1599192

 cAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGccc                                                                                        <  2:5336027/93‑1 (MQ=255)

  aGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCt                                                                                       >  2:2212616/1‑93 (MQ=255)

  aGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCt                                                                                       >  1:4310527/1‑93 (MQ=255)

   gCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCtt                                                                                      <  1:5738315/93‑1 (MQ=255)

   gCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCtt                                                                                      <  2:5103936/93‑1 (MQ=255)

    cGAAAACTGTCGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCttt                                                                                     <  2:5048640/93‑1 (MQ=255)

    cGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCttt                                                                                     <  2:4149274/93‑1 (MQ=255)

    cGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCttt                                                                                     <  2:4710313/93‑1 (MQ=255)

      aaaaCTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTtct                                                                                   >  2:5483880/1‑93 (MQ=255)

       aaaCTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTtctc                                                                                  <  2:227061/93‑1 (MQ=255)

        aaCTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTcc                                                                                 >  2:4317122/1‑93 (MQ=255)

         aCTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCa                                                                                <  1:674917/93‑1 (MQ=255)

         aCTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCa                                                                                <  1:1747466/93‑1 (MQ=255)

           tGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGc                                                                              >  1:2443654/1‑93 (MQ=255)

           tGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGc                                                                              <  1:5865212/93‑1 (MQ=255)

            gacgacGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCa                                                                             >  1:5592406/1‑93 (MQ=255)

             acgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCaa                                                                            >  1:2345750/1‑93 (MQ=255)

             acgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCaa                                                                            >  2:4511707/1‑93 (MQ=255)

              cgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAAt                                                                           <  1:4512018/93‑1 (MQ=255)

              cgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAAt                                                                           <  1:3697374/93‑1 (MQ=255)

              cgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAAt                                                                           <  2:2779372/93‑1 (MQ=255)

              cgacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAAt                                                                           <  2:4768335/93‑1 (MQ=255)

               gacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATc                                                                          >  2:4062184/1‑93 (MQ=255)

               gacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATc                                                                          >  2:2037572/1‑93 (MQ=255)

               gacgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATc                                                                          <  1:6230523/93‑1 (MQ=255)

                atgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATcg                                                                         >  1:4687488/3‑93 (MQ=255)

                acgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATcg                                                                         <  1:4122700/93‑1 (MQ=255)

                 cgTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATcgc                                                                        <  2:2387393/93‑1 (MQ=255)

                   tAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGa                                                                      >  1:1819333/1‑93 (MQ=255)

                    aaTTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGac                                                                     >  2:3500714/1‑93 (MQ=255)

                     aTTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGaca                                                                    >  1:414799/1‑93 (MQ=255)

                      ttGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGacac                                                                   <  2:6091019/93‑1 (MQ=255)

                        gAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACtt                                                                 <  1:3881718/93‑1 (MQ=255)

                        gAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACtt                                                                 <  2:2943275/93‑1 (MQ=255)

                        gAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACtt                                                                 <  2:5943827/93‑1 (MQ=255)

                            ttCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCaa                                                             <  1:5553312/93‑1 (MQ=255)

                                 atTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACgtg                                                        >  2:4506019/1‑93 (MQ=255)

                                 atTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACgtg                                                        <  2:5819980/93‑1 (MQ=255)

                                 atTCCAGACAGCCTTCAAAGCGACAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACgtg                                                        >  2:5609305/1‑93 (MQ=255)

                                  ttCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACgtgt                                                       <  1:4920911/93‑1 (MQ=255)

                                   tCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTc                                                      >  1:3460478/1‑93 (MQ=255)

                                     cAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCTAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAg                                                    <  1:4790932/93‑1 (MQ=255)

                                     cAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAg                                                    <  2:1575032/93‑1 (MQ=255)

                                      aGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCGAACGTGTCAgg                                                   <  1:3968542/93‑1 (MQ=255)

                                      aGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAgg                                                   >  2:2648103/1‑93 (MQ=255)

                                      aGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTAAAACGTGTCAgg                                                   >  2:5707890/1‑93 (MQ=255)

                                       gACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGc                                                  >  1:3363739/1‑93 (MQ=255)

                                       gACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGc                                                  >  1:884264/1‑93 (MQ=255)

                                       gACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGc                                                  >  1:4461004/1‑93 (MQ=255)

                                        aCAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGcc                                                 >  1:1143411/1‑93 (MQ=255)

                                        aCAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGcc                                                 <  2:815575/93‑1 (MQ=255)

                                          aGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCga                                               <  1:321638/93‑1 (MQ=255)

                                          aGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCga                                               >  2:3359341/1‑93 (MQ=255)

                                           gCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCgag                                              >  1:5175657/1‑93 (MQ=255)

                                            ccTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCgaga                                             <  1:702033/93‑1 (MQ=255)

                                            ccTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCgaga                                             <  1:3341524/93‑1 (MQ=255)

                                            ccTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCgaga                                             <  2:3625641/93‑1 (MQ=255)

                                            ccTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCgaga                                             <  1:5201417/93‑1 (MQ=255)

                                             cTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAc                                            <  2:2337659/93‑1 (MQ=255)

                                              ttCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACg                                           <  1:3554112/93‑1 (MQ=255)

                                              ttCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTATCAAACGTGTCAGGCCGAGACg                                           >  2:4984835/1‑93 (MQ=255)

                                                cAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGAt                                         >  2:3886419/1‑93 (MQ=255)

                                                cAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGAt                                         <  1:1673999/93‑1 (MQ=255)

                                                cAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGAt                                         <  2:3574001/93‑1 (MQ=255)

                                                 aaaGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATc                                        >  2:918574/1‑93 (MQ=255)

                                                   aGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGc                                      >  2:711611/1‑93 (MQ=255)

                                                    gCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCt                                     >  1:2187767/1‑93 (MQ=255)

                                                    gCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCt                                     <  1:127312/93‑1 (MQ=255)

                                                    gCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCt                                     >  1:1494459/1‑93 (MQ=255)

                                                    gCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCt                                     <  1:4941947/93‑1 (MQ=255)

                                                     cGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTg                                    <  1:5353389/93‑1 (MQ=255)

                                                     cGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTg                                    <  1:1446890/93‑1 (MQ=255)

                                                       agaATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAt                                  >  1:2403837/1‑93 (MQ=255)

                                                        gaATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAtc                                 >  1:2837901/1‑93 (MQ=255)

                                                         aaTTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAtct                                <  2:3062746/93‑1 (MQ=255)

                                                         aaTTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAtct                                >  2:3141394/1‑93 (MQ=255)

                                                         aaTTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAtct                                >  1:5278177/1‑93 (MQ=255)

                                                         aaTTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAtct                                >  1:5119243/1‑93 (MQ=255)

                                                         aaTTAATCTGTACGCGAATAATGCCGAGCTGACGCCCTTCCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAtct                                <  1:2994422/93‑1 (MQ=255)

                                                           ttAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCg                              >  1:3711024/1‑93 (MQ=255)

                                                           ttAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCg                              >  2:2985263/1‑93 (MQ=255)

                                                              aTCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGctc                           <  2:1385933/93‑1 (MQ=255)

                                                              aTCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGctc                           <  1:3640871/93‑1 (MQ=255)

                                                               tCTGTCCGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAACCGCGACACTTTCAACCGTGTCAGGCCGAGACGATCGCTGATCTCGctct                          <  1:857949/93‑1 (MQ=255)

                                                               tCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGctct                          >  1:1604140/1‑93 (MQ=255)

                                                                cTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTg                         >  2:1010137/1‑93 (MQ=255)

                                                                cTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTg                         <  1:5385422/93‑1 (MQ=255)

                                                                 tGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTgg                        <  2:1963583/93‑1 (MQ=255)

                                                                    aCGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTc                     >  2:6175083/1‑93 (MQ=255)

                                                                     cgcgAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCa                    >  1:268826/1‑93 (MQ=255)

                                                                     cgcgAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCa                    >  2:5208342/1‑93 (MQ=255)

                                                                        gAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGcc                 >  1:2042995/1‑93 (MQ=255)

                                                                          ataatGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCcg               >  1:3755075/1‑93 (MQ=255)

                                                                          ataatGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCcg               >  2:1510751/1‑93 (MQ=255)

                                                                             atGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCcgtcg            >  1:4244971/1‑93 (MQ=255)

                                                                              tGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCcgtcgt           <  2:3285094/93‑1 (MQ=255)

                                                                                  ggACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATa       <  1:2541659/93‑1 (MQ=255)

                                                                                  ggACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATa       <  2:3750160/93‑1 (MQ=255)

                                                                                  ggACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATa       <  1:1022028/93‑1 (MQ=255)

                                                                                       gATGCCCGTTCGCCAGCAATCGCGACACTTCCAAACTTGTCAGGCCGTGACGATCGCTGATCTCGCTCTGGTTCAGCCCGTCGCGATAGTaaa  >  2:6304458/1‑93 (MQ=255)

GCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAA  >  NC_000913/1599013‑1599192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACC  >  NC_000913/1599009‑1599195

TGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGAC                                                                                               <  SRR4302533.5839842/94‑1 (MQ=60)

 GTTGCAGCGAAAACTGACGACGTAATTTAGTTTGATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACG                                                                                              <  SRR4302533.2274787/94‑1 (MQ=60)

 GTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACG                                                                                              <  SRR4302533.2743578/94‑1 (MQ=60)

 GTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACG                                                                                              <  SRR4302533.3299106/94‑1 (MQ=60)

 GTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACG                                                                                              >  SRR4302533.3888722/1‑94 (MQ=60)

   TGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCC                                                                                            >  SRR4302533.2226286/1‑94 (MQ=60)

    GCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCC                                                                                           <  SRR4302533.5677426/94‑1 (MQ=60)

     CAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCT                                                                                          >  SRR4302533.4336476/1‑94 (MQ=60)

       GCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTT                                                                                        <  SRR4302533.1130496/94‑1 (MQ=60)

       GCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTT                                                                                        <  SRR4302533.5772948/94‑1 (MQ=60)

        CGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTC                                                                                       >  SRR4302533.2339199/1‑94 (MQ=60)

           AAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCC                                                                                    >  SRR4302533.4209637/1‑94 (MQ=60)

            AACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCA                                                                                   >  SRR4302533.2886192/1‑94 (MQ=60)

             ACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAG                                                                                  <  SRR4302533.4159481/94‑1 (MQ=60)

             ACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAG                                                                                  <  SRR4302533.1336878/94‑1 (MQ=60)

             ACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAG                                                                                  >  SRR4302533.6336527/1‑94 (MQ=60)

             ACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAG                                                                                  <  SRR4302533.1758103/94‑1 (MQ=60)

             ACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAG                                                                                  <  SRR4302533.678904/94‑1 (MQ=60)

              CTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGC                                                                                 >  SRR4302533.2458738/1‑94 (MQ=60)

               TGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCA                                                                                >  SRR4302533.5626188/1‑94 (MQ=60)

               TGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCA                                                                                >  SRR4302533.4414511/1‑94 (MQ=60)

               TGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCA                                                                                <  SRR4302533.5900672/94‑1 (MQ=60)

                GACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAA                                                                               >  SRR4302533.2360269/1‑94 (MQ=60)

                GACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAA                                                                               >  SRR4302533.96121/1‑94 (MQ=60)

                GACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAAATCCGGAATGACGCCCTTTCTTCAGCAA                                                                               >  SRR4302533.3962525/1‑94 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             >  SRR4302533.5357950/1‑94 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             <  SRR4302533.4821686/94‑1 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             <  SRR4302533.4539339/94‑1 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             <  SRR4302533.3720086/94‑1 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             <  SRR4302533.5166879/94‑1 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             >  SRR4302533.3877735/1‑94 (MQ=60)

                  CGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             >  SRR4302533.1647173/1‑94 (MQ=60)

                  CGACGGAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATC                                                                             <  SRR4302533.5208003/94‑1 (MQ=60)

                   GATGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCG                                                                            >  SRR4302533.4715899/1‑94 (MQ=60)

                   GACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCG                                                                            <  SRR4302533.910724/94‑1 (MQ=60)

                   GACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCG                                                                            <  SRR4302533.753941/94‑1 (MQ=60)

                   GACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCG                                                                            >  SRR4302533.3613286/1‑94 (MQ=60)

                   GACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCG                                                                            <  SRR4302533.6267837/94‑1 (MQ=60)

                    ACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGC                                                                           <  SRR4302533.4147730/94‑1 (MQ=60)

                     CGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCG                                                                          >  SRR4302533.752049/1‑94 (MQ=60)

                      GTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGA                                                                         >  SRR4302533.1830448/1‑94 (MQ=60)

                        AATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACA                                                                       >  SRR4302533.157482/1‑94 (MQ=60)

                        AATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACA                                                                       >  SRR4302533.417291/1‑94 (MQ=60)

                        AATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACA                                                                       >  SRR4302533.4249948/1‑94 (MQ=60)

                        AATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACA                                                                       >  SRR4302533.1689759/1‑94 (MQ=60)

                         ATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACAC                                                                      >  SRR4302533.2726712/1‑94 (MQ=60)

                          TTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACT                                                                     <  SRR4302533.4822549/94‑1 (MQ=60)

                           TGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTT                                                                    >  SRR4302533.2400485/1‑94 (MQ=60)

                           TGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTT                                                                    <  SRR4302533.3721662/94‑1 (MQ=60)

                           TGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTT                                                                    <  SRR4302533.5583911/94‑1 (MQ=60)

                            GAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTT                                                                   <  SRR4302533.3905407/94‑1 (MQ=60)

                             AGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTC                                                                  >  SRR4302533.60898/1‑94 (MQ=60)

                              GTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCA                                                                 <  SRR4302533.3263876/94‑1 (MQ=60)

                              GTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCA                                                                 <  SRR4302533.1278945/94‑1 (MQ=60)

                               TTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAA                                                                >  SRR4302533.4969107/1‑94 (MQ=60)

                                TTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAA                                                               <  SRR4302533.5586849/94‑1 (MQ=60)

                                  CATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACG                                                             >  SRR4302533.2458538/1‑94 (MQ=60)

                                   ATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGT                                                            >  SRR4302533.1736294/1‑94 (MQ=60)

                                      TTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTC                                                         >  SRR4302533.3481839/1‑94 (MQ=60)

                                      TTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTC                                                         <  SRR4302533.4950720/94‑1 (MQ=60)

                                      TTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTC                                                         >  SRR4302533.2569974/1‑94 (MQ=60)

                                       TCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCA                                                        >  SRR4302533.4339863/1‑94 (MQ=60)

                                        CCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAG                                                       >  SRR4302533.682693/1‑94 (MQ=60)

                                         CAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCTAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGG                                                      <  SRR4302533.4819958/94‑1 (MQ=60)

                                         CAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGG                                                      >  SRR4302533.2450261/1‑94 (MQ=60)

                                         CAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGG                                                      <  SRR4302533.4736582/94‑1 (MQ=60)

                                          AGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCGAACGTGTCAGGC                                                     <  SRR4302533.3992715/94‑1 (MQ=60)

                                          AGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGC                                                     >  SRR4302533.4487987/1‑94 (MQ=60)

                                          AGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGC                                                     >  SRR4302533.1803603/1‑94 (MQ=60)

                                          AGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGC                                                     >  SRR4302533.889457/1‑94 (MQ=60)

                                          AGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGC                                                     >  SRR4302533.3384522/1‑94 (MQ=60)

                                           GACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCC                                                    <  SRR4302533.886857/94‑1 (MQ=60)

                                           GACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCC                                                    >  SRR4302533.1150230/1‑94 (MQ=60)

                                           GACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCC                                                    >  SRR4302533.3079119/1‑94 (MQ=60)

                                           GACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCC                                                    >  SRR4302533.5620567/1‑94 (MQ=60)

                                             CAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGA                                                  <  SRR4302533.3469878/94‑1 (MQ=60)

                                              AGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAG                                                 >  SRR4302533.5206918/1‑94 (MQ=60)

                                              AGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAG                                                 >  SRR4302533.5439551/1‑94 (MQ=60)

                                              AGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAG                                                 <  SRR4302533.323604/94‑1 (MQ=60)

                                              AGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAG                                                 >  SRR4302533.2115123/1‑94 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAG                                               >  SRR4302533.5037716/1‑94 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAC                                               <  SRR4302533.3362177/94‑1 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAC                                               <  SRR4302533.706187/94‑1 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAC                                               <  SRR4302533.3517636/94‑1 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAC                                               <  SRR4302533.5232768/94‑1 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAC                                               <  SRR4302533.4097100/94‑1 (MQ=60)

                                                CCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGAC                                               >  SRR4302533.3758850/1‑94 (MQ=60)

                                                 CTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGAGACACTTTCACACGTGTCAGGCCGAGACG                                              >  SRR4302533.2231007/1‑94 (MQ=60)

                                                 CTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCACCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACG                                              >  SRR4302533.3662781/1‑94 (MQ=60)

                                                  TTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGA                                             <  SRR4302533.3576008/94‑1 (MQ=60)

                                                  TTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGA                                             >  SRR4302533.5405584/1‑94 (MQ=60)

                                                   TCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGAT                                            <  SRR4302533.5380532/94‑1 (MQ=60)

                                                    CAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATC                                           <  SRR4302533.1684117/94‑1 (MQ=60)

                                                    CAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATC                                           >  SRR4302533.1885907/1‑94 (MQ=60)

                                                     AAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCG                                          >  SRR4302533.4095748/1‑94 (MQ=60)

                                                     AAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCG                                          >  SRR4302533.5030846/1‑94 (MQ=60)

                                                      AAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGC                                         >  SRR4302533.5845472/1‑94 (MQ=60)

                                                       AGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCT                                        >  SRR4302533.2201244/1‑94 (MQ=60)

                                                       AGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCT                                        >  SRR4302533.1503465/1‑94 (MQ=60)

                                                        GCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTG                                       <  SRR4302533.128109/94‑1 (MQ=60)

                                                        GCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTG                                       <  SRR4302533.4971863/94‑1 (MQ=60)

                                                        GCGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTG                                       >  SRR4302533.335646/1‑94 (MQ=60)

                                                         CGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGA                                      <  SRR4302533.5385519/94‑1 (MQ=60)

                                                         CGAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGA                                      <  SRR4302533.1455621/94‑1 (MQ=60)

                                                          GAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAT                                     >  SRR4302533.4567882/1‑94 (MQ=60)

                                                          GAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAT                                     <  SRR4302533.5291961/94‑1 (MQ=60)

                                                          GAGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGAT                                     >  SRR4302533.2418683/1‑94 (MQ=60)

                                                           AGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATC                                    >  SRR4302533.2855367/1‑94 (MQ=60)

                                                           AGAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATC                                    >  SRR4302533.1334262/1‑94 (MQ=60)

                                                            GAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCT                                   >  SRR4302533.5150174/1‑94 (MQ=60)

                                                            GAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCT                                   <  SRR4302533.2555488/94‑1 (MQ=60)

                                                            GAATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCT                                   >  SRR4302533.5309817/1‑94 (MQ=60)

                                                             AATTAATCTGTACGCGAATAATGCCGAGCTGACGCCCTTCCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTC                                  <  SRR4302533.3012731/94‑1 (MQ=60)

                                                              ATTAATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCG                                 >  SRR4302533.3733813/1‑94 (MQ=60)

                                                                 AATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTC                              <  SRR4302533.6257969/94‑1 (MQ=60)

                                                                 AATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTC                              >  SRR4302533.4529111/1‑94 (MQ=60)

                                                                 AATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTC                              <  SRR4302533.3382493/94‑1 (MQ=60)

                                                                  ATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCT                             >  SRR4302533.1613853/1‑94 (MQ=60)

                                                                  ATCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCT                             <  SRR4302533.3663261/94‑1 (MQ=60)

                                                                   TCTGTCCGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAACCGCGACACTTTCAACCGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTG                            <  SRR4302533.862975/94‑1 (MQ=60)

                                                                   TCTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTG                            <  SRR4302533.3679529/94‑1 (MQ=60)

                                                                    CTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGG                           <  SRR4302533.1457497/94‑1 (MQ=60)

                                                                    CTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGG                           <  SRR4302533.5417779/94‑1 (MQ=60)

                                                                    CTGTACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGG                           >  SRR4302533.3392894/1‑94 (MQ=60)

                                                                        ACGCGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCA                       >  SRR4302533.270457/1‑94 (MQ=60)

                                                                         CGCGAATAGTGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAG                      <  SRR4302533.5207424/94‑1 (MQ=60)

                                                                           CGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCC                    >  SRR4302533.3727225/1‑94 (MQ=60)

                                                                           CGAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCC                    >  SRR4302533.2055590/1‑94 (MQ=60)

                                                                            GAATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGACC                   >  SRR4302533.2043770/1‑94 (MQ=60)

                                                                             AATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCG                  >  SRR4302533.3778089/1‑94 (MQ=60)

                                                                              ATAATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGT                 >  SRR4302533.3839747/1‑94 (MQ=60)

                                                                                AATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCG               >  SRR4302533.4270602/1‑94 (MQ=60)

                                                                                AATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCG               >  SRR4302533.4270603/1‑94 (MQ=60)

                                                                                AATGCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCG               <  SRR4302533.2661288/94‑1 (MQ=60)

                                                                                   GCCGGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGA            >  SRR4302533.2932953/1‑94 (MQ=60)

                                                                                      GGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAG         >  SRR4302533.5705401/1‑94 (MQ=60)

                                                                                      GGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAG         <  SRR4302533.2557305/94‑1 (MQ=60)

                                                                                      GGACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAG         <  SRR4302533.1028033/94‑1 (MQ=60)

                                                                                        ACTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTA       <  SRR4302533.4643030/94‑1 (MQ=60)

                                                                                         CTGACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAA      <  SRR4302533.1505821/94‑1 (MQ=60)

                                                                                           GACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAA    >  SRR4302533.4571223/1‑94 (MQ=60)

                                                                                            ACGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAAC   <  SRR4302533.2767297/94‑1 (MQ=60)

                                                                                             CGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACC  >  SRR4302533.1429345/1‑94 (MQ=60)

                                                                                             CGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACC  <  SRR4302533.5567818/94‑1 (MQ=60)

TGTTGCAGCGAAAACTGACGACGTAATTGAGTTTCATATTCCAGACAGCCTTCAAAGCGAGAATTAATCTGTACGCGAATAATGCCGGACTGATGCCCTTTCTCCAGCAATCGCGACACTTTCAAACGTGTCAGGCCGAGACGATCGCTGATCTCGCTCTGGGTCAGCCCGTCGTGATAGTAAAACC  >  NC_000913/1599009‑1599195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`