Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,599,943 G→A A144T (GCC→ACC)  b1513 → putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,599,9430GA100.0% 620.4 / NA 173A144T (GCC→ACC) b1513putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (95/78);  total (95/78)

Only 100 of 178 total aligned reads displayed.

AAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCT  >  NC_000913/1599853‑1600032
                                                                                          |                                                                                         
aaaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCAcc                                                                                         <  1:4031607/93‑1 (MQ=255)
 aaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCg                                                                                        <  2:1516302/93‑1 (MQ=255)
 aaCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCg                                                                                        <  1:4116537/93‑1 (MQ=255)
  aCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGa                                                                                       >  2:4988153/1‑93 (MQ=255)
  aCAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGa                                                                                       >  2:5193891/1‑93 (MQ=255)
   cAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGAt                                                                                      <  2:5177775/93‑1 (MQ=255)
    aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                     >  1:4039623/1‑93 (MQ=255)
    aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                     <  2:2845617/93‑1 (MQ=255)
    aGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATc                                                                                     <  2:3050916/93‑1 (MQ=255)
     gCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCg                                                                                    >  2:578253/1‑93 (MQ=255)
       tctcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGcc                                                                                  >  1:6003618/1‑93 (MQ=255)
       tctcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGcc                                                                                  >  1:6177302/1‑93 (MQ=255)
        ctcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCa                                                                                 >  1:2721667/1‑93 (MQ=255)
        ctcCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCa                                                                                 >  2:4855037/1‑93 (MQ=255)
          ccATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaa                                                                               <  1:4355329/93‑1 (MQ=255)
          ccATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaa                                                                               >  2:6020054/1‑93 (MQ=255)
          ccATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaa                                                                               >  1:3503068/1‑93 (MQ=255)
           cATGCAGAAAATGAAGAGCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCaaag                                                                              <  1:2621592/93‑2 (MQ=255)
             tGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtgg                                                                            >  2:5641592/1‑93 (MQ=255)
               cAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGTCAGGATCGCTGGATGTCACCGATCTCCAAAtggtg                                                                          <  1:5002601/93‑1 (MQ=255)
               cAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAtggtg                                                                          >  1:3155558/1‑93 (MQ=255)
                 gAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGa                                                                        <  2:3728455/93‑1 (MQ=255)
                  aaaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaa                                                                       >  2:5552638/1‑93 (MQ=255)
                  aaaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaa                                                                       >  2:4795767/1‑93 (MQ=255)
                  aaaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaa                                                                       >  2:1923494/1‑93 (MQ=255)
                   aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                      <  2:2062544/93‑1 (MQ=255)
                   aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                      <  1:3062420/93‑1 (MQ=255)
                   aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                      <  2:3437751/93‑1 (MQ=255)
                   aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                      <  2:3476520/93‑1 (MQ=255)
                   aaaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGaaa                                                                      >  1:2112309/1‑93 (MQ=255)
                    aaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAt                                                                     >  1:824193/1‑93 (MQ=255)
                    aaTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAt                                                                     >  1:74394/1‑93 (MQ=255)
                     aTGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtc                                                                    >  1:4641329/1‑93 (MQ=255)
                       gaagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcct                                                                  >  2:2173803/1‑93 (MQ=255)
                        aagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctc                                                                 <  1:3584192/93‑1 (MQ=255)
                        aagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctc                                                                 <  2:5437732/93‑1 (MQ=255)
                        aagaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAtcctc                                                                 <  2:1045702/93‑1 (MQ=255)
                          gaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                               >  2:3531975/1‑93 (MQ=255)
                          gaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                               >  2:4509094/1‑93 (MQ=255)
                          gaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                               <  1:1755312/93‑1 (MQ=255)
                          gaaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcg                                                               <  1:2704456/93‑1 (MQ=255)
                           aaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgc                                                              <  2:1036586/93‑1 (MQ=255)
                           aaCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgc                                                              >  2:2737420/1‑93 (MQ=255)
                           aaCTTGCTGGCAGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgc                                                              <  1:560238/93‑1 (MQ=255)
                            aCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCcgcg                                                             <  2:1994247/93‑1 (MQ=255)
                             cTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCgg                                                            <  2:1827071/93‑1 (MQ=255)
                              ttGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCggg                                                           >  2:2551743/1‑93 (MQ=255)
                              ttGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCggg                                                           >  2:3693660/1‑93 (MQ=255)
                               tGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGc                                                          <  2:5802895/93‑1 (MQ=255)
                               tGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGAATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGc                                                          <  2:3398860/93‑1 (MQ=255)
                                gCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCt                                                         >  2:5945858/1‑93 (MQ=255)
                                 cTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTg                                                        >  1:2897624/1‑93 (MQ=255)
                                 cTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTg                                                        <  1:1917216/93‑1 (MQ=255)
                                  tGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGa                                                       >  2:3433446/1‑93 (MQ=255)
                                   ggcggcGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGAt                                                      <  2:4477617/93‑1 (MQ=255)
                                    gcggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATg                                                     >  1:5797263/1‑93 (MQ=255)
                                    gcggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATg                                                     <  1:1149861/93‑1 (MQ=255)
                                     cggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgc                                                    <  2:2005387/93‑1 (MQ=255)
                                     cggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgc                                                    <  1:4686254/93‑1 (MQ=255)
                                     cggcgCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATgc                                                    <  1:3144635/93‑1 (MQ=255)
                                        cgcTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGcgcg                                                 <  2:3985336/93‑1 (MQ=255)
                                          cTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGAc                                               >  1:3718969/1‑93 (MQ=255)
                                           tGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACt                                              >  1:3357065/1‑93 (MQ=255)
                                           tGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACt                                              <  1:5719377/93‑1 (MQ=255)
                                           tGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACt                                              >  2:3277445/1‑93 (MQ=255)
                                             ggCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTcg                                            >  2:1003363/1‑93 (MQ=255)
                                              gCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTcgc                                           >  2:4314958/1‑93 (MQ=255)
                                                 gCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGa                                        <  2:1213313/93‑1 (MQ=255)
                                                   cAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGAtt                                      <  2:2573991/93‑1 (MQ=255)
                                                    aGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTc                                     <  1:5520940/93‑1 (MQ=255)
                                                     gTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCt                                    <  1:1690339/93‑1 (MQ=255)
                                                     gTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCt                                    <  1:6026837/93‑1 (MQ=255)
                                                      tttGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTg                                   >  2:3080789/1‑93 (MQ=255)
                                                        tGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGAt                                 <  1:2551525/93‑1 (MQ=255)
                                                         gATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATc                                >  1:2154234/1‑93 (MQ=255)
                                                         gATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATc                                >  2:2303196/1‑93 (MQ=255)
                                                          aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                               <  2:756756/93‑1 (MQ=255)
                                                          aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                               >  1:2414694/1‑93 (MQ=255)
                                                          aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                               <  2:5765324/93‑1 (MQ=255)
                                                          aTCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATcc                               >  2:1694942/1‑93 (MQ=255)
                                                            cTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTc                             <  2:3012287/93‑1 (MQ=255)
                                                             tGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCg                            >  1:3806663/1‑93 (MQ=255)
                                                              gCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGa                           >  1:1227927/1‑93 (MQ=255)
                                                                aTAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATTCTCGATg                         >  2:3899408/1‑93 (MQ=255)
                                                                 tAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                        >  1:1478128/1‑93 (MQ=255)
                                                                 tAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                        >  1:1549693/1‑93 (MQ=255)
                                                                 tAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGa                        >  1:2401306/1‑93 (MQ=255)
                                                                   gTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAAc                      <  1:3130804/93‑1 (MQ=255)
                                                                       ggCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAc                  <  2:979438/93‑1 (MQ=255)
                                                                        gCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAcc                 <  2:1134658/93‑1 (MQ=255)
                                                                         cAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCg                >  2:4060932/1‑93 (MQ=255)
                                                                           ggATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGcc              >  1:2752305/1‑93 (MQ=255)
                                                                             aTCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGACCCTGCCGCCTc            <  2:6007652/93‑1 (MQ=255)
                                                                               cGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGc          <  2:4087244/93‑1 (MQ=255)
                                                                                gctggctgTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCt         >  2:6276006/1‑93 (MQ=255)
                                                                                   ggATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAc      <  2:4038245/93‑1 (MQ=255)
                                                                                      tGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTAcccc   <  1:624916/93‑1 (MQ=255)
                                                                                       gTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCt  >  1:3682331/1‑93 (MQ=255)
                                                                                       gTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCt  >  1:6274316/1‑93 (MQ=255)
                                                                                       gTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCt  >  2:3950930/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCT  >  NC_000913/1599853‑1600032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCG  >  NC_000913/1599850‑1600035
                                                                                             |                                                                                            
AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCA                                                                                              <  SRR4302533.5740486/94‑1 (MQ=60)
  AAAACAGCTCTCCATGCAGAAAATGAAGAACTCGCTGGCGGCGCTGAGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACC                                                                                            <  SRR4302533.5427406/94‑1 (MQ=60)
   AAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCG                                                                                           <  SRR4302533.4056148/94‑1 (MQ=60)
    AACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGA                                                                                          <  SRR4302533.4141544/94‑1 (MQ=60)
    AACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGA                                                                                          >  SRR4302533.6300345/1‑94 (MQ=60)
      CAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATC                                                                                        <  SRR4302533.1627066/94‑1 (MQ=60)
      CAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATC                                                                                        >  SRR4302533.4064205/1‑94 (MQ=60)
         CTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCC                                                                                     >  SRR4302533.6039994/1‑94 (MQ=60)
         CTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCC                                                                                     >  SRR4302533.6214413/1‑94 (MQ=60)
          TCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCA                                                                                    >  SRR4302533.2738369/1‑94 (MQ=60)
            TCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAA                                                                                  >  SRR4302533.3524665/1‑94 (MQ=60)
             CCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAT                                                                                 <  SRR4302533.4381618/94‑1 (MQ=60)
              CATGCAGAAAATGAAGAGCTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAAGG                                                                                <  SRR4302533.2637682/94‑1 (MQ=60)
               ATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGG                                                                               <  SRR4302533.1904347/94‑1 (MQ=60)
                 GCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTG                                                                             >  SRR4302533.3174958/1‑94 (MQ=60)
                  CAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGTCAGGATCGCTGGATGTCACCGATCTCCAAATGGTGG                                                                            <  SRR4302533.5032924/94‑1 (MQ=60)
                   AGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGA                                                                           >  SRR4302533.6045466/1‑94 (MQ=60)
                   AGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGA                                                                           >  SRR4302533.5945965/1‑94 (MQ=60)
                     AAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAA                                                                         >  SRR4302533.2125338/1‑94 (MQ=60)
                      AAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAT                                                                        >  SRR4302533.74876/1‑94 (MQ=60)
                      AAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAT                                                                        >  SRR4302533.829030/1‑94 (MQ=60)
                      AAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAT                                                                        <  SRR4302533.3081220/94‑1 (MQ=60)
                      AAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAAT                                                                        >  SRR4302533.4536285/1‑94 (MQ=60)
                       AATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATC                                                                       >  SRR4302533.4669449/1‑94 (MQ=60)
                        ATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCC                                                                      <  SRR4302533.5565587/94‑1 (MQ=60)
                         TGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCT                                                                     <  SRR4302533.5703906/94‑1 (MQ=60)
                          GAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTC                                                                    >  SRR4302533.2748601/1‑94 (MQ=60)
                           AAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCC                                                                   <  SRR4302533.783574/94‑1 (MQ=60)
                           AAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCC                                                                   <  SRR4302533.3606265/94‑1 (MQ=60)
                            AGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCG                                                                  >  SRR4302533.2109006/1‑94 (MQ=60)
                             GAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGC                                                                 <  SRR4302533.2721046/94‑1 (MQ=60)
                             GAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGC                                                                 <  SRR4302533.1766004/94‑1 (MQ=60)
                              AACTTGCTGGCAGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCG                                                                <  SRR4302533.563563/94‑1 (MQ=60)
                                CTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGG                                                              >  SRR4302533.375228/1‑94 (MQ=60)
                                CTTGCTGGCGGCGCCGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATAGTGGAAATCGTCCGCGGG                                                              >  SRR4302533.5858065/1‑94 (MQ=60)
                                   GCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTG                                                           >  SRR4302533.2915436/1‑94 (MQ=60)
                                    CTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGA                                                          <  SRR4302533.1928921/94‑1 (MQ=60)
                                     TGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGAT                                                         <  SRR4302533.4374036/94‑1 (MQ=60)
                                      GGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATG                                                        >  SRR4302533.5832287/1‑94 (MQ=60)
                                       GCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGC                                                       <  SRR4302533.1156729/94‑1 (MQ=60)
                                        CGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCG                                                      >  SRR4302533.150495/1‑94 (MQ=60)
                                        CGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCG                                                      <  SRR4302533.4714642/94‑1 (MQ=60)
                                        CGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCG                                                      <  SRR4302533.3163970/94‑1 (MQ=60)
                                        CGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGAGGCG                                                      >  SRR4302533.3070790/1‑94 (MQ=60)
                                            GCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGAC                                                  >  SRR4302533.3741797/1‑94 (MQ=60)
                                            GCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGAC                                                  >  SRR4302533.3354530/1‑94 (MQ=60)
                                            GCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGAC                                                  >  SRR4302533.214684/1‑94 (MQ=60)
                                             CTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACT                                                 >  SRR4302533.4070817/1‑94 (MQ=60)
                                             CTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACT                                                 >  SRR4302533.3377809/1‑94 (MQ=60)
                                              TGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTC                                                <  SRR4302533.5753912/94‑1 (MQ=60)
                                                       AGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCT                                       <  SRR4302533.5554272/94‑1 (MQ=60)
                                                        GTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTG                                      <  SRR4302533.6063360/94‑1 (MQ=60)
                                                        GTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTG                                      <  SRR4302533.1700578/94‑1 (MQ=60)
                                                          TTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGAT                                    <  SRR4302533.2900552/94‑1 (MQ=60)
                                                           TGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATC                                   >  SRR4302533.2167500/1‑94 (MQ=60)
                                                           TGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATC                                   <  SRR4302533.2567228/94‑1 (MQ=60)
                                                           TGATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGAGCCGCGACTCGCGGATTCTGATC                                   >  SRR4302533.3168787/1‑94 (MQ=60)
                                                            GATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCC                                  >  SRR4302533.2429608/1‑94 (MQ=60)
                                                             ATCTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCT                                 >  SRR4302533.2578623/1‑94 (MQ=60)
                                                               CTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCG                               >  SRR4302533.4406896/1‑94 (MQ=60)
                                                               CTGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCG                               >  SRR4302533.3829954/1‑94 (MQ=60)
                                                                TGCATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGA                              >  SRR4302533.1235280/1‑94 (MQ=60)
                                                                   ATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGA                           >  SRR4302533.2416136/1‑94 (MQ=60)
                                                                   ATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGA                           <  SRR4302533.2363629/94‑1 (MQ=60)
                                                                   ATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGA                           >  SRR4302533.1559032/1‑94 (MQ=60)
                                                                   ATAGTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGA                           >  SRR4302533.1487057/1‑94 (MQ=60)
                                                                      GTCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACC                        <  SRR4302533.3150055/94‑1 (MQ=60)
                                                                       TCTGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCT                       >  SRR4302533.611176/1‑94 (MQ=60)
                                                                         TGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAC                     >  SRR4302533.3720015/1‑94 (MQ=60)
                                                                         TGGCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTAC                     <  SRR4302533.1452996/94‑1 (MQ=60)
                                                                           GCAGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCG                   <  SRR4302533.3518605/94‑1 (MQ=60)
                                                                             AGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCC                 >  SRR4302533.3898424/1‑94 (MQ=60)
                                                                             AGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCC                 >  SRR4302533.689482/1‑94 (MQ=60)
                                                                             AGGATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCC                 >  SRR4302533.2769183/1‑94 (MQ=60)
                                                                               GATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTC               >  SRR4302533.2555178/1‑94 (MQ=60)
                                                                                ATCGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCG              <  SRR4302533.4845868/94‑1 (MQ=60)
                                                                                  CGCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCT            <  SRR4302533.8410/94‑1 (MQ=60)
                                                                                   GCTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTT           <  SRR4302533.5068420/94‑1 (MQ=60)
                                                                                    CTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTA          >  SRR4302533.3149813/1‑94 (MQ=60)
                                                                                    CTGGATGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTA          >  SRR4302533.2701986/1‑94 (MQ=60)
                                                                                         TGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCT     <  SRR4302533.628585/94‑1 (MQ=60)
                                                                                         TGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCT     >  SRR4302533.6311790/1‑94 (MQ=60)
                                                                                         TGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCT     >  SRR4302533.3704963/1‑94 (MQ=60)
                                                                                         TGTCACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCT     <  SRR4302533.3656983/94‑1 (MQ=60)
                                                                                            CACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCG  >  SRR4302533.5116981/1‑94 (MQ=60)
                                                                                            CACCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCG  <  SRR4302533.3581792/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAAAAACAGCTCTCCATGCAGAAAATGAAGAACTTGCTGGCGGCGCTGGGCTGCCAGTTTGATCTGCATAGTCTGGCAGGATCGCTGGATGTCGCCGATCGCCAAATGGTGGAAATCCTCCGCGGGCTGATGCGCGACTCGCGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCG  >  NC_000913/1599850‑1600035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑